The Crop Journal最新文献_第5页

Effect of rice cultivar on greenhouse-gas emissions from rice–fish co-culture 水稻品种对稻鱼共养温室气体排放的影响

The Crop Journal Pub Date : 2024-05-29 DOI: 10.1016/j.cj.2024.04.011

Kexin Xie, Mengjie Wang, Xiaodan Wang, Fengbo Li, Chunchun Xu, Jinfei Feng, Fuping Fang

引用次数: 0

ZmCER1, a putative ECERIFERUM 1 protein in maize, functions in cuticular wax biosynthesis and bulliform cell development ZmCER1 是玉米中一种推定的 ECERIFERUM 1 蛋白，在角质蜡生物合成和牛皮状细胞发育中发挥作用

The Crop Journal Pub Date : 2024-05-27 DOI: 10.1016/j.cj.2024.05.001

Yan Zhao, Qingzhi Liu, Xueran Wang, Weixiao Zhang, Wei Xu, Yongzhong Zhang, Baoshen Liu

引用次数: 0

GSW3.1, a novel gene controlling grain size and weight in rice 控制水稻粒度和粒重的新基因 GSW3.1

The Crop Journal Pub Date : 2024-05-27 DOI: 10.1016/j.cj.2024.05.002

Yifei Jiang, Mingao Zhou, Simin Ke, Xiaoxiao Deng, Yangsheng Li

引用次数: 0

Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides 玉米基因 ZmSBR1 的自然变异赋予幼苗对疣镰刀菌的抗性

The Crop Journal Pub Date : 2024-05-27 DOI: 10.1016/j.cj.2024.05.003

Yunxia Song, Peipei Ma, Jingyang Gao, Chaopei Dong, Zhao Wang, Yifan Luan, Jiafa Chen, Doudou Sun, Pei Jing, Xuecai Zhang, Weibin Song, Zijian Zhou, Jianyu Wu

{"title":"Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides","authors":"Yunxia Song, Peipei Ma, Jingyang Gao, Chaopei Dong, Zhao Wang, Yifan Luan, Jiafa Chen, Doudou Sun, Pei Jing, Xuecai Zhang, Weibin Song, Zijian Zhou, Jianyu Wu","doi":"10.1016/j.cj.2024.05.003","DOIUrl":"https://doi.org/10.1016/j.cj.2024.05.003","url":null,"abstract":"Maize seedling blight caused by is a widely occurring maize disease, but the genetics and mechanisms of resistance are not well understood. In this study, GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs, associated with seedling blight resistance. These methods identified 49 and 36 genes, respectively. Functional verification of candidate gene identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with , compared with the WT. The length of the stem rot lesion caused by increased by 86% in mutant seedlings, and the relative length of the adult plant stalk rot increased by 35% in mutant plants compared to the wild type after inoculation with . Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type, synthesis of secondary metabolites associated with resistance was reduced, and the immune response triggered by PAMP decreased, resulting in decreased resistance of mutant maize seedlings. Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype. A functional PCR marker was developed. The results demonstrated that conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141517717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa 开发用于自交系苜蓿高效基因组编辑的单转录本 CRISPR/Cas9 工具包

The Crop Journal Pub Date : 2024-05-03 DOI: 10.1016/j.cj.2024.04.001

Haixia Zhao, Siyi Zhao, Yingping Cao, Xiping Jiang, Lijuan Zhao, Zhimeng Li, Mengqi Wang, Ruijuan Yang, Chuanen Zhou, Zhaoming Wang, Feng Yuan, Dongmei Ma, Hao Lin, Wenwen Liu, Chunxiang Fu

{"title":"Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa","authors":"Haixia Zhao, Siyi Zhao, Yingping Cao, Xiping Jiang, Lijuan Zhao, Zhimeng Li, Mengqi Wang, Ruijuan Yang, Chuanen Zhou, Zhaoming Wang, Feng Yuan, Dongmei Ma, Hao Lin, Wenwen Liu, Chunxiang Fu","doi":"10.1016/j.cj.2024.04.001","DOIUrl":"https://doi.org/10.1016/j.cj.2024.04.001","url":null,"abstract":"Alfalfa (. L.) is a globally significant autotetraploid legume forage crop. However, despite its importance, establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge. In this study, we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa, a variety widely cultivated in Northeast China. Subsequently, we created a single transcript CRISPR/Cas9 (CRISPR_2.0) toolkit, incorporating multiplex gRNAs, designed for gene editing in Gongnong 1. Both and gRNA scaffolds were under the control of the promoter, a widely employed polymerase II constitutive promoter known for strong transgene expression in dicots. To assess the toolkit’s efficiency, we targeted , a gene associated with a recognizable multifoliate phenotype. Utilizing the CRISPR_2.0 toolkit, we directed editing at two sites in the wild-type Gongnong 1. Results indicated a 35.1% occurrence of editing events all in target 2 alleles, while no mutations were detected at target 1 in the transgenic-positive lines. To explore more efficient sgRNAs, we developed a rapid, reliable screening system based on mediated hairy root transformation, incorporating the visible reporter MtLAP1. This screening system demonstrated that most purple visible hairy roots underwent gene editing. Notably, sgRNA3, with an 83.0% editing efficiency, was selected using the visible hairy root system. As anticipated, tetra-allelic homozygous mutations exhibited a clear multifoliate phenotype. These lines demonstrated an average crude protein yield increase of 21.5% compared to trifoliolate alfalfa. Our findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel semi-dominant allele of the transmembrane NAC transcription factor ZmNTL2 reduces the size of multiple maize organs 跨膜 NAC 转录因子 ZmNTL2 的新型半显性等位基因会缩小玉米多个器官的尺寸

The Crop Journal Pub Date : 2024-05-03 DOI: 10.1016/j.cj.2024.04.002

Chuan Li, Yun Bai, Yuwei Hou, Siyu Wang, Yue Xin, Tao Yu, Jing Wang, Xiaowei Liu, Hongyang Yi, Chao Xia, Hai Lan, Moju Cao

{"title":"A novel semi-dominant allele of the transmembrane NAC transcription factor ZmNTL2 reduces the size of multiple maize organs","authors":"Chuan Li, Yun Bai, Yuwei Hou, Siyu Wang, Yue Xin, Tao Yu, Jing Wang, Xiaowei Liu, Hongyang Yi, Chao Xia, Hai Lan, Moju Cao","doi":"10.1016/j.cj.2024.04.002","DOIUrl":"https://doi.org/10.1016/j.cj.2024.04.002","url":null,"abstract":"Maize plant architecture influences planting density and, in turn, grain yield. Most of the plant architecture-related traits can be described as organ size. We describe a miniature maize mutant, (), which exhibits reduced size of multiple organs and exhibits a semi-dominant monofactorial inheritance characteristic. Positional cloning confirmed that a 4-bp deletion in the NAC TF with transmembrane motif 1-Like (NTL) gene , denoted as 2, confers the mutation. qRT-PCR showed that was expressed in all tested tissues. ZmNTL2 functions as a transcriptional activator and is located in both the nucleus and biomembranes. The mutation does not affect the mRNA abundance of locus, but it does result in the loss of transmembrane domain and confines the ZmNTL2 protein to the nucleus. Knocking out has no effect on maize organ size development, indicating that the 4-bp deletion might be a gain-of-function mutation in organ size regulation. Combining transcriptome sequencing with cytokinin and auxin content determination suggests that the decreased organ size may be possibly mediated by changes in hormone homeostasis.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild soybean (Glycine soja) transcription factor GsWRKY40 plays positive roles in plant salt tolerance 野生大豆（Glycine soja）转录因子 GsWRKY40 在植物耐盐性中发挥积极作用

The Crop Journal Pub Date : 2024-05-03 DOI: 10.1016/j.cj.2024.03.011

Minglong Li, Man Xue, Huiying Ma, Peng Feng, Tong Chen, Xiaohuan Sun, Qiang Li, Xiaodong Ding, Shuzhen Zhang, Jialei Xiao

{"title":"Wild soybean (Glycine soja) transcription factor GsWRKY40 plays positive roles in plant salt tolerance","authors":"Minglong Li, Man Xue, Huiying Ma, Peng Feng, Tong Chen, Xiaohuan Sun, Qiang Li, Xiaodong Ding, Shuzhen Zhang, Jialei Xiao","doi":"10.1016/j.cj.2024.03.011","DOIUrl":"https://doi.org/10.1016/j.cj.2024.03.011","url":null,"abstract":"Wild soybean (), a relative of cultivated soybean, shows high adaptability to adverse environmental conditions. We identified and characterized a wild soybean transcription factor gene, , that promotes plant salt stress. was highly expressed in wild soybean roots and was up-regulated by salt treatment. GsWRKY40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional activation. Mutation and overexpression of altered salt tolerance of plants. To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance, we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach. The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation (co-IP), GST pull-down, and bimolecular fluorescence complementation (BiFC) techniques. Intriguingly, co-overexpression of and resulted in higher salt tolerance and lower ROS levels than overexpression of or in composite soybean plants, suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production. qRT-PCR data indicated that the expression level of gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92, which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment. Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding expression and reducing ROS levels, providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"191 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptome sequencing reveals the mechanism regulating rice plumule development 单细胞转录组测序揭示水稻胚珠发育的调控机制

The Crop Journal Pub Date : 2024-05-01 DOI: 10.1016/j.cj.2024.04.009

Mingdong Zhu, Meng Zhang, Kunyong Huang, Feifei Lu, Hong Wang, Shaolu Zhao, Yinghong Yu, Shaoqing Tang, Haining Wu, Peisong Hu, Xiangjin Wei

引用次数: 0

A rice exocyst subunit gene SEC3A is required for anther dehiscence 花药开裂需要水稻外囊亚基基因 SEC3A

The Crop Journal Pub Date : 2024-05-01 DOI: 10.1016/j.cj.2024.04.010

Ranran Tu, Hong Wang, Zhihao Sun, Qinwen Zou, Jiajun Wu, Duo Wu, Nan Wang, Guanghua He

引用次数: 0

Overexpression of the peroxidase gene ZmPRX1 increases maize seedling drought tolerance by promoting root development and lignification 过表达过氧化物酶基因 ZmPRX1 可通过促进根系发育和木质化提高玉米幼苗的耐旱性

The Crop Journal Pub Date : 2024-05-01 DOI: 10.1016/j.cj.2024.04.008

Xiuzhen Zhai, Xiaocui Yan, Tinashe Zenda, Nan Wang, Anyi Dong, Qian Yang, Yuan Zhong, Yue Xing, Huijun Duan

引用次数: 0