The Crop JournalPub Date : 2024-07-01DOI: 10.1016/j.cj.2024.06.010
Chi Li, Yingqi Huang, W. Shang, Jieyin Chen, S. Klosterman, Krishna V. Subbarao, Jun Qin, Xiaoping Hu
{"title":"A glycine-rich nuclear effector VdCE51 of Verticillium dahliae suppresses plant immune responses by inhibiting the accumulation of GhTRXH2","authors":"Chi Li, Yingqi Huang, W. Shang, Jieyin Chen, S. Klosterman, Krishna V. Subbarao, Jun Qin, Xiaoping Hu","doi":"10.1016/j.cj.2024.06.010","DOIUrl":"https://doi.org/10.1016/j.cj.2024.06.010","url":null,"abstract":"","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"61 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fine mapping of two recessive genes TaFLA1 and TaSPL8 controlling flag leaf angle in bread wheat","authors":"Qiushi Wang, Jiaxing Bai, Hongchun Xiong, Yongdun Xie, Chaojie Wang, Jiayu Gu, Linshu Zhao, Huiyuan Li, Jinfeng Zhang, Shirong Zhao, Yuping Ding, Zhengwu Fang, Huijun Guo, Luxiang Liu","doi":"10.1016/j.cj.2024.06.001","DOIUrl":"https://doi.org/10.1016/j.cj.2024.06.001","url":null,"abstract":"Flag leaf angle is one of the key target traits in high yield wheat breeding. A smaller flag leaf angle reduces shading and enables plants to grow at a higher density, which increases yield. Here we identified a mutant, , with an 84.34%–89.35% smaller flag leaf angle compared with the wild type. The mutant also had an abnormal lamina joint and no ligule or auricle. Genetic analysis indicated that the ligule was controlled by two recessive genes, which were mapped to chromosomes 2AS and 2DL. The mutant allele on chromosome 2AS was named , and it was fine mapped to a 1 Mb physical interval. The mutant allele on chr. 2DL was identified as , a novel allele of with a missense mutation in the second exon, which was used to develop a cleaved amplified polymorphic sequence marker. F and F lines derived from crosses between Jing411 and were genotyped to investigate interactions between the and alleles. Plants with the genotype had 58.41%–82.76% smaller flag leaf angles, 6.4%–24.9% shorter spikes, and a greater spikelet density (0.382 more spikelets per cm) compared with the wild type. Plants with the genotype had 52.62%–82.24% smaller flag leaf angles and no differences in plant height or spikelet density compared with the wild type. plants produced erect leaves with an abnormal lamina joint. The two alleles had dosage effects on ligule formation and flag leaf angle, but no significant effect on thousand-grain weight. The mutant alleles provide novel resources for improvement of wheat plant architecture.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-06-22DOI: 10.1016/j.cj.2024.05.014
Bogui Pan, Yixia Cai, Kunzheng Cai, Jihui Tian, Wei Wang
{"title":"Optimized silicon fertilization regime weakens cadmium translocation and increases its biotransformation in rice tissues","authors":"Bogui Pan, Yixia Cai, Kunzheng Cai, Jihui Tian, Wei Wang","doi":"10.1016/j.cj.2024.05.014","DOIUrl":"https://doi.org/10.1016/j.cj.2024.05.014","url":null,"abstract":"In acidic paddy fields of South China, rice ( L.) faces the dual challenges of cadmium (Cd) toxicity and silicon (Si) deficiency. Although previous studies have highlighted the functions of Si application timing and strategies in mitigating Cd-stressed rice, the precise mechanisms underlying the health restoration of Cd-toxic rice and the assurance of grain safety remain elusive. This study explored Cd translocation and detoxification in the shoots of rice regulated by various Si fertilization regimes: Si(T) (all Si added before transplanting), Si(J) (all Si added at jointing), and Si(TJ) (half Si added both before transplanting and at jointing). The findings revealed that the regime of Si(TJ) was more beneficial to rice health and grain safety than Si(T) and Si(J). The osmotic regulators such as proline, soluble sugars, and soluble proteins were significantly boosted by Si(TJ) compared to other Si treatments, and which enhanced membrane integrity, balanced intracellular pH, and increased Cd tolerance of rice. Furthermore, Si(TJ) was more effective than Si(T) and Si(J) on the Cd sequestration in the cell wall, Cd bio-passivation, and the down-regulated expression of the Cd transport genes. The concentrations of Cd in the xylem and phloem treated with Si(TJ) were reduced significantly. Additionally, Si(TJ) facilitated much more Cd bound with the outer layer proteins of grains, and promoted Cd chelation and complexation by phytic acid, phenolics, and flavonoids. Overall, Si (TJ) outperformed Si(T) and Si(J) in harmonizing the phycological processes, inhibiting Cd translocation, and enhancing Cd detoxification in rice plant. Thereby the split Si application strategy offers potential for reducing Cd toxicity in rice grain.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141506959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-06-22DOI: 10.1016/j.cj.2024.05.015
Ping Li, Xia Zhang, Wuzhong Yin, Yang Shui, Jie Zhang, Nana Xu, Dasong Bai, Qingxiong Huang, Yuanyuan Li, Pan Qi, Xing Li, Qianlong Li, Shihong Yang, Guotao Yang, Hong Chen, Peng Qin, Yungao Hu, Youlin Peng
{"title":"OsFK1 encodes C-14 sterol reductase, which is involved in sterol biosynthesis and affects premature aging of leaves in rice","authors":"Ping Li, Xia Zhang, Wuzhong Yin, Yang Shui, Jie Zhang, Nana Xu, Dasong Bai, Qingxiong Huang, Yuanyuan Li, Pan Qi, Xing Li, Qianlong Li, Shihong Yang, Guotao Yang, Hong Chen, Peng Qin, Yungao Hu, Youlin Peng","doi":"10.1016/j.cj.2024.05.015","DOIUrl":"https://doi.org/10.1016/j.cj.2024.05.015","url":null,"abstract":"The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids (BR) and sterols, as well as plant development. , a member of the sterol biosynthesis pathway located in the endoplasmic reticulum (ER), encodes C-14 sterol reductase. However, there is little research on the function of C-14 sterol reductase in rice. Compared with the wild type, an mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage, and abnormal development of leaf veins during the tillering stage. The mutant showed signs of aberrant PCD, as evidenced by TUNEL staining. This suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the mutant. The mutant also showed decreased chlorophyll content and aberrant chloroplast structure. Sequencing of the mutant allele revealed a non-synonymous G to A mutation in the final intron, leading to early termination. Here, we identified the allele, cloned it by Mutmap sequencing, and verified it by complementation. HPLC-MS/MS assays demonstrated that the mutation caused lower phytosterol levels. These findings showed that the allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-05-31DOI: 10.1016/j.cj.2024.05.005
Chengke Pang, Jun Yu, Liang Zhang, Min Tang, Hongfang Liu, Ying Cai, Feng Chen, Jiefu Zhang, Wei Hua, Xiaodong Wang, Ming Zheng
{"title":"BnaC03.BIN2 regulates plant height by affecting the main inflorescence length and first effective branch height in Brassica napus L.","authors":"Chengke Pang, Jun Yu, Liang Zhang, Min Tang, Hongfang Liu, Ying Cai, Feng Chen, Jiefu Zhang, Wei Hua, Xiaodong Wang, Ming Zheng","doi":"10.1016/j.cj.2024.05.005","DOIUrl":"https://doi.org/10.1016/j.cj.2024.05.005","url":null,"abstract":"Rapeseed ( L.) is one of the main oil crops in the world, and increasing its yield is of great significance for ensuring the safety of edible oil. Presently, improving rapeseed plant architecture is an effective way to increase rapeseed yield with higher planting density. However, the regulatory mechanism of rapeseed plant architecture is poorly understood. In this study, a dwarf rapeseed mutant () is obtained by ethyl methane sulfonate (EMS)-mutagenesis. The decrease in plant height of is mainly caused by the reduction in main inflorescence length and first effective branch height and controlled by a single semi-dominant gene. The hybrid plants (F) show a semi-dwarf phenotype. Through map-based cloning and transgenic assay, we confirm that the nonsynonymous single nucleotide variant (SNV) (C to T) in , which is homologous with () , is responsible for the dwarfism of . BnaC03.BIN2 interacts with BnaBZR1/BES1 and involves in brassinosteroids (BRs) signal transduction. Proline to Leucine substitution in 284 (P284L) enhances the protein stability of BnaC03.bin2-D, disrupts BRs signal transduction and affects the expression of genes regulating cell division, leading to dwarfism of . This study provides a new insight for the mechanism of rapeseed plant height regulation and creates an elite germplasm that can be used for genetic improvement of rapeseed architecture.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141517719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-05-31DOI: 10.1016/j.cj.2024.04.012
Zimeng Liang, Jingyi Feng, Jiayu Li, Yangyang Tang, Tiankang He, Vinay Nangia, Yang Liu
{"title":"Grain-filling strategies of wheat of contrasting grain sizes under various planting patterns and irrigation levels","authors":"Zimeng Liang, Jingyi Feng, Jiayu Li, Yangyang Tang, Tiankang He, Vinay Nangia, Yang Liu","doi":"10.1016/j.cj.2024.04.012","DOIUrl":"https://doi.org/10.1016/j.cj.2024.04.012","url":null,"abstract":"In a study comparing grain filling and yield in a large- and a small-grain-size wheat cultivar under two planting patterns and two irrigation regimes, plastic-covered ridge and furrow planting with sprinkler irrigation increased grain filling and yield in both cultivars. The largest contributors to grain yield were an extended active grain-filling period in Shuangda 1 and an increased mean grain-filling rate in XN538.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"130 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"OsPPG encodes a pseudouridine-5′-phosphate glycosidase and regulates rice spikelet development","authors":"Rui Chen, Dagang Tian, Yarong Lin, Jiaqing Huang, Qinqin Yang, Yupei Li, Huaqing Liu, Shaohua Yang, Jingyang Chen, Shufen Zhou, Feng Wang, Shubiao Zhang","doi":"10.1016/j.cj.2024.05.004","DOIUrl":"https://doi.org/10.1016/j.cj.2024.05.004","url":null,"abstract":"Florets are the basic structural units of spikelets, and their morphogenesis determines the yield and quality of rice grains. However, whether and how pseudouridine-5′-phosphate glycosidase participates in rice spikelet development remains an open question. In this study, we identified a novel gene, , which encodes a peroxisome-localized pseudouridine-5′-phosphate glycosidase and regulates the development of rice spikelets. mutants exhibited abnormal sterile lemma, lemma, palea, lodicule, stamens, and pistils; male sterility; shorter panicles; and reduced plant height. was found to regulate several genes, thereby affecting the morphogenesis of rice spikelets. Furthermore, metabolomics revealed that the gene was involved in the decomposition of pseudouridine via the pyrimidine metabolism pathway and may affect the jasmonic acid signaling pathway. These results suggest that OsPPG is a key regulator of rice spikelet development.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}