Korean Journal of Parasitology最新文献_第5页

Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line. 刚地弓形虫通过内质网应激源线粒体途径诱导人小肠上皮细胞凋亡

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.573

Hao Wang, Chunchao Li, Wei Ye, Zhaobin Pan, Jinhui Sun, Mingzhu Deng, Weiqiang Zhan, Jiaqi Chu

{"title":"Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line.","authors":"Hao Wang, Chunchao Li, Wei Ye, Zhaobin Pan, Jinhui Sun, Mingzhu Deng, Weiqiang Zhan, Jiaqi Chu","doi":"10.3347/kjp.2021.59.6.573","DOIUrl":"https://doi.org/10.3347/kjp.2021.59.6.573","url":null,"abstract":"Toxoplasma gondii, an intracellular protozoan parasite that infects one-third of the world's population, has been reported to hijack host cell apoptotic machinery and promote either an anti- or proapoptotic program depending on the parasite virulence and load and the host cell type. However, little is known about the regulation of human FHs 74 small intestinal epithelial cell viability in response to T. gondii infection. Here we show that T. gondii RH strain tachyzoite infection or ESP treatment of FHs 74 Int cells induced apoptosis, mitochondrial dysfunction and ER stress in host cells. Pretreatment with 4-PBA inhibited the expression or activation of key molecules involved in ER stress. In addition, both T. gondii and ESP challenge-induced mitochondrial dysfunction and cell death were dramatically suppressed in 4-PBA pretreated cells. Our study indicates that T. gondii infection induced ER stress in FHs 74 Int cells, which induced mitochondrial dysfunction followed by apoptosis. This may constitute a potential molecular mechanism responsible for the foodborne parasitic disease caused by T. gondii.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8b/f5/kjp-59-6-573.PMC8721304.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Report of Chigger Mites on the Striped Field Mouse, Apodemus agrarius, in Southwest China. 西南地区黑线姬鼠恙螨调查报告。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.625

Yan-Ling Chen, Xian-Guo Guo, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Xiao-Bin Huang, Ti-Jun Qian

{"title":"A Report of Chigger Mites on the Striped Field Mouse, Apodemus agrarius, in Southwest China.","authors":"Yan-Ling Chen, Xian-Guo Guo, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Xiao-Bin Huang, Ti-Jun Qian","doi":"10.3347/kjp.2021.59.6.625","DOIUrl":"https://doi.org/10.3347/kjp.2021.59.6.625","url":null,"abstract":"Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (PM=3.4%), overall mean abundance (MA=0.36 mites/host) and overall mean intensity (MI=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with CSS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/6c/kjp-59-6-625.PMC8721306.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Failure of Repeated MDA with Albendazole for Trichuriasis Control in Schoolchildren of the Yangon Region, Myanmar. 在缅甸仰光地区的学龄儿童中使用阿苯达唑反复 MDA 控制毛滴虫病的失败案例。

IF 1.4 4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.607

Sooji Hong, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Woon-Mok Sohn, Sung-Jong Hong, Jin-Youp Ryu, Hyun-Seung Kim, Mun-Hyoo Jeong, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai

{"title":"Failure of Repeated MDA with Albendazole for Trichuriasis Control in Schoolchildren of the Yangon Region, Myanmar.","authors":"Sooji Hong, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Woon-Mok Sohn, Sung-Jong Hong, Jin-Youp Ryu, Hyun-Seung Kim, Mun-Hyoo Jeong, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai","doi":"10.3347/kjp.2021.59.6.607","DOIUrl":"10.3347/kjp.2021.59.6.607","url":null,"abstract":"Soil-transmitted helminth (STH) infections are still a considerable challenge in Myanmar. We undertook a control program for STH infections (especially Trichuris trichiura) among schoolchildren in Myanmar using mass drug administration (MDA) and health education. Around 1,700 schoolchildren from 15 primary schools in 3 suburban districts (Shwe Pyi Thar, Twantay, and Kyauktan) of the Yangon Region were subjected in this study during 2017-2019. All of the schoolchildren in each school were orally administered albendazole (400 mg in a single dose) 2, 3, and 4 times a year in 2017, 2018, and 2019, respectively. The results revealed that the egg positive rate of any intestinal helminths (including STH) was reduced from 37.6% (649/1,724) in 2017 to 22.8% (352/1,542) in 2019. The egg positive rate of Ascaris lumbricoides was decreased remarkably from 23.3% (402/1,724) in 2017 to 3.6% (56/1,542) in 2019. However, that of T. trichiura was only slightly reduced from 26.9% (464/1,724) in 2017 to 20.2% (312/1,542) in 2019. The intensity of infection with A. lumbricoides and T. trichiura was both more or less reduced, and the proportion of light infection cases with A. lumbricoides and T. trichiura increased from 35.6% in 2017 to 64.3% in 2019 and from 70.3% in 2017 to 81.7% in 2019, respectively. The results indicated that repeated MDAs (2-4 times a year for 3 years) using albendazole on schoolchildren in Myanmar failed to control T. trichiura infection. For a successful control of trichuriasis in Myanmar, new MDA strategies, using a modified albendazole regimen (multiple daily doses for 2 or 3 days) or an alternative anthelmintic drug, such as oxantel pamoate, is strongly recommended.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/13/kjp-59-6-607.PMC8721305.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Human Case of Lumbosacral Canal Sparganosis in China. 人腰骶管缩窄症1例。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.635

Jian-Feng Fan, Sheng Huang, Jing Li, Ren-Jun Peng, He Huang, Xi-Ping Ding, Li-Ping Jiang, Jian Xi

{"title":"A Human Case of Lumbosacral Canal Sparganosis in China.","authors":"Jian-Feng Fan, Sheng Huang, Jing Li, Ren-Jun Peng, He Huang, Xi-Ping Ding, Li-Ping Jiang, Jian Xi","doi":"10.3347/kjp.2021.59.6.635","DOIUrl":"https://doi.org/10.3347/kjp.2021.59.6.635","url":null,"abstract":"In this study, we intended to describe a human case of lumbosacral canal sparganosis in People's Republic of China (China). A 56-year-old man was admitted to Xiangya Hospital Central South University in Changsha, Hunan province, China after having an experience of perianal pain for a week. An enhancing mass, a tumor clinically suggested, was showed at the S1-S2 level of the lumbosacral spine by the examination of magnetic resonance imaging (MRI) with gadolinium contrast. The patient was received the laminectomy from S1 to S2, and an ivory-white living worm was detected in inferior margin of L5. In ELISA-test with cerebrospinal fluid (CSF) and serum samples, anti-sparganum antibodies were detected. He had a ingesting history of undercooked frog meat in his youth. By the present study, a human case of spinal sparganosis invaded in lumbosacral canal at the S1-S2 level was diagnosed in China. Although the surgical removal of larvae is known to be the best way of treatment for sparganosis, we administered the high-dosage of praziquantel, albendazole and dexamethasone to prevent the occurrence of another remain worms in this study.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/47/kjp-59-6-635.PMC8721303.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Enterocytozoon bieneusi Genotypes and Infections in the Horses in Korea. 韩国马的双胞虫基因型和感染。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.639

Haeseung Lee, Seung-Hun Lee, Yu-Ran Lee, Ha-Young Kim, Bo-Youn Moon, Jee Eun Han, Man Hee Rhee, Oh-Deog Kwon, Dongmi Kwak

引用次数: 2

Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis. 巨噬细胞参与阴道毛滴虫感染的前列腺癌细胞增殖

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.557

Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu

{"title":"Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis.","authors":"Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu","doi":"10.3347/kjp.2021.59.6.557","DOIUrl":"10.3347/kjp.2021.59.6.557","url":null,"abstract":"Macrophages play a key role in chronic inflammation, and are the most abundant immune cells in the tumor microenvironment. We investigated whether an interaction between inflamed prostate cancer cells stimulated with Trichomonas vaginalis and macrophages stimulates the proliferation of the cancer cells. Conditioned medium was prepared from T. vaginalis-infected (TCM) and uninfected (CM) mouse prostate cancer (PCa) cell line (TRAMP-C2 cells). Thereafter conditioned medium was prepared from macrophages (J774A.1 cell line) after incubation with CM (MCM) or TCM (MTCM). When TRAMP-C2 cells were stimulated with T. vaginalis, protein and mRNA levels of CXCL1 and CCL2 increased, and migration of macrophages toward TCM was more extensive than towards CM. Macrophages stimulated with TCM produced higher levels of CCL2, IL-6, TNF-α, their mRNAs than macrophages stimulated with CM. MTCM stimulated the proliferation and invasiveness of TRAMP-C2 cells as well as the expression of cytokine receptors (CCR2, GP130, CXCR2). Importantly, blocking of each cytokine receptors with anti-cytokine receptor antibody significantly reduced the proliferation and invasiveness of TRAMP-C2 cells. We conclude that inflammatory mediators released by TRAMP-C2 cells in response to infection by T. vaginalis stimulate the migration and activation of macrophages and the activated macrophages stimulate the proliferation and invasiveness of the TRAMP-C2 cells via cytokine-cytokine receptor binding. Our results therefore suggested that macrophages contribute to the exacerbation of PCa due to inflammation of prostate cancer cells reacted with T. vaginalis.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/7a/kjp-59-6-557.PMC8721302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39639446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis. 阴道毛滴虫感染小鼠前列腺癌细胞的增殖研究。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.547

Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu

{"title":"Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis.","authors":"Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu","doi":"10.3347/kjp.2021.59.6.547","DOIUrl":"10.3347/kjp.2021.59.6.547","url":null,"abstract":"Our objective was to investigate whether inflammatory microenvironment induced by Trichomonas vaginalis infection can stimulate proliferation of prostate cancer (PCa) cells in vitro and in vivo mouse experiments. The production of CXCL1 and CCL2 increased when cells of the mouse PCa cells (TRAMP-C2 cell line) were infected with live T. vaginalis. T. vaginalis-conditioned medium (TCM) prepared from co-culture of PCa cells and T. vaginalis increased PCa cells migration, proliferation and invasion. The cytokine receptors (CXCR2, CCR2, gp130) were expressed higher on the PCa cells treated with TCM. Pretreatment of PCa cells with antibodies to these cytokine receptors significantly reduced the proliferation, mobility and invasiveness of PCa cells, indicating that TCM has its effect through cytokine-cytokine receptor signaling. In C57BL/6 mice, the prostates injected with T. vaginalis mixed PCa cells were larger than those injected with PCa cells alone after 4 weeks. Expression of epithelial-mesenchymal transition markers and cyclin D1 in the prostate tissue injected with T. vaginalis mixed PCa cells increased than those of PCa cells alone. Collectively, it was suggested that inflammatory reactions by T. vaginalis-stimulated PCa cells increase the proliferation and invasion of PCa cells through cytokine-cytokine receptor signaling pathways.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4d/13/kjp-59-6-547.PMC8721307.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39639084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen. 显示弓形虫ROP4抗原的病毒样颗粒检测弓形虫感染。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.565

Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan

{"title":"Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen.","authors":"Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan","doi":"10.3347/kjp.2021.59.6.565","DOIUrl":"10.3347/kjp.2021.59.6.565","url":null,"abstract":"Toxoplasma gondii ME49 infections are typically diagnosed by serological tests. However, serological diagnosis of RH strain-induced toxoplasmosis remains unknown. In order to develop seradiagnosis of above 2 kinds of infections, we generated recombinant virus-like particles (VLPs) displaying the T. gondii rhoptry protein 4 (ROP4) and evaluated their potential in T. gondii ME49 or RH strain infection diagnostics. Mice were orally infected with either the tachyzoites of T. gondii (RH) or cysts of T. gondii (ME49) at various dosages, and sera were collected at regular intervals. ELISA-based serological tests were performed to assess IgG, IgM, and IgA antibody responses against ROP4 VLP antigen and tissue lysate antigen (TLA). Compared to TLA, IgG, IgM, and IgA levels to ROP4 VLP antigen were significantly higher in the sera of T. gondii RH-infected mice 1 and 2 week post-infection (PI). T. gondii-specific IgG antibody was detected at 1, 2, 4, and 8 week PI in the T. gondii ME49-infected mice with infection dose-dependent manner. These results indicated that the ROP4 VLP antigen was highly sensitive antigens detecting T. gondii RH and ME49 antibodies at an early stage.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d1/f5/kjp-59-6-565.PMC8721301.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent Spatial and Temporal Trends of Malaria in Korea. 最近韩国疟疾的时空变化趋势。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.585

Yeong Hoon Kim, Hye-Jin Ahn, Dongjae Kim, Sung-Jong Hong, Tong-Soo Kim, Ho-Woo Nam

引用次数: 0

Cryptocotyle lata (Trematoda: Heterophyidae) Adult from a Korean Raccoon Dog, Nyctereutes procyonoides koreensis. 韩国貉成虫隐子叶(吸虫目:异虫科)。

4区医学

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI: 10.3347/kjp.2021.59.6.645

Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park

引用次数: 0