Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen.

IF 1.4 4区医学 Q3 PARASITOLOGY

Korean Journal of Parasitology Pub Date : 2021-12-01 Epub Date: 2021-12-22 DOI:10.3347/kjp.2021.59.6.565

Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan

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引用次数: 1

Abstract

Toxoplasma gondii ME49 infections are typically diagnosed by serological tests. However, serological diagnosis of RH strain-induced toxoplasmosis remains unknown. In order to develop seradiagnosis of above 2 kinds of infections, we generated recombinant virus-like particles (VLPs) displaying the T. gondii rhoptry protein 4 (ROP4) and evaluated their potential in T. gondii ME49 or RH strain infection diagnostics. Mice were orally infected with either the tachyzoites of T. gondii (RH) or cysts of T. gondii (ME49) at various dosages, and sera were collected at regular intervals. ELISA-based serological tests were performed to assess IgG, IgM, and IgA antibody responses against ROP4 VLP antigen and tissue lysate antigen (TLA). Compared to TLA, IgG, IgM, and IgA levels to ROP4 VLP antigen were significantly higher in the sera of T. gondii RH-infected mice 1 and 2 week post-infection (PI). T. gondii-specific IgG antibody was detected at 1, 2, 4, and 8 week PI in the T. gondii ME49-infected mice with infection dose-dependent manner. These results indicated that the ROP4 VLP antigen was highly sensitive antigens detecting T. gondii RH and ME49 antibodies at an early stage.

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显示弓形虫ROP4抗原的病毒样颗粒检测弓形虫感染。

弓形虫ME49感染通常通过血清学检测来诊断。然而，RH菌株诱导的弓形虫病的血清学诊断仍然未知。为了开发上述两种感染的血清学诊断，我们产生了显示弓形虫杆状蛋白4（ROP4）的重组病毒样颗粒（VLP），并评估了它们在弓形虫ME49或RH菌株感染诊断中的潜力。用不同剂量的弓形虫速殖子（RH）或弓形虫囊肿（ME49）口服感染小鼠，并定期收集血清。进行基于ELISA的血清学测试以评估针对ROP4 VLP抗原和组织裂解物抗原（TLA）的IgG、IgM和IgA抗体反应。与TLA相比，感染后1周和2周（PI），弓形虫RH感染小鼠血清中ROP4 VLP抗原的IgG、IgM和IgA水平显著较高。在感染弓形虫ME49的小鼠中，在第1、2、4和8周PI时检测到弓形虫特异性IgG抗体，其感染呈剂量依赖性。这些结果表明，ROP4-VLP抗原是早期检测弓形虫RH和ME49抗体的高灵敏度抗原。

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来源期刊

Korean Journal of Parasitology PARASITOLOGY-

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Korean Journal of Parasitology is the official journal paperless, on-line publication after Vol. 53, 2015 of The Korean Society for Parasitology and Tropical Medicine. Abbreviated title is ‘Korean J Parasitol’. It was launched in 1963. It contains original articles, case reports, brief communications, reviews or mini-reviews, book reviews, and letters to the editor on parasites of humans and animals, vectors, host-parasite relationships, zoonoses, and tropical medicine. It is published bimonthly in February, April, June, August, October, and December each year. Supplement numbers are at times published. All of the manuscripts are peer-reviewed.