Hong Lin, Song Zhao, Yuying Ye, Lei Shao, Nizhen Jiang, Kun Yang
{"title":"A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti.","authors":"Hong Lin, Song Zhao, Yuying Ye, Lei Shao, Nizhen Jiang, Kun Yang","doi":"10.3347/kjp.2022.60.3.201","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.201","url":null,"abstract":"<p><p>Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/µl B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"201-205"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/80/d9/kjp-60-3-201.PMC9256288.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park
{"title":"Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia.","authors":"Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park","doi":"10.3347/kjp.2022.60.3.163","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.163","url":null,"abstract":"<p><p>Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-32P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"163-172"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/88/kjp-60-3-163.PMC9256289.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40466674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seropositivity Rates of Strongyloides stercoralis Antibody in the Southeastern Region of Republic of Korea: A Single-Center Retrospective Study.","authors":"Taehwa Kim, Seungjin Lim","doi":"10.3347/kjp.2022.60.3.181","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.181","url":null,"abstract":"<p><p>Strongyloides stercoralis infection is not endemic in the Republic of Korea (Korea) with a positivity rate of <1% in stool examination. However, there is a risk of hyperinfection in immunosuppressed individuals. It is necessary to determine the seropositivity of S. stercoralis antibodies in Korea. This study investigated the seropositivity of S. stercoralis antibodies in the southeastern area of Korea. From January 2019 to June 2021, serum samples were collected from participants who visited the study center in the southeastern region of Korea for routine health check-ups. We determined serum levels of specific anti-Strongyloides IgG antibodies in 834 samples by enzyme-linked immunosorbent assay. We observed that 92 samples (11.0%) tested showed a positive response. The age of the participants was 51±10.7 years, and 43.4% of them were men. The antibody positivity rate based on the location of the participants' residence were 12.3% (Gyoungsangnam-do), 10.2% (Busan), and 10.1% (Ulsan), respectively. Total eosinophil count was associated with positive test results (154.8±152.0 per mm3 versus 202.1±178.9 per mm3, P=0.006). Logistic regression analysis revealed that blood eosinophil count, age above 50 years, and residence in Sacheon were factors associated with the positive status of S. stercoralis antibody. Our finding suggests that it is necessary to test for S. stercoralis in actual clinical settings in Korea.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"181-185"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/82/kjp-60-3-181.PMC9256283.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Status of Helminthic Infections in Residents around River Basins in the Republic of Korea for 10 Years (2011-2020).","authors":"Myoung-Ro Lee, Hee-Eun Shin, Seon-Ok Back, Young-Ju Lee, Hee-Il Lee, Jung-Won Ju","doi":"10.3347/kjp.2022.60.3.187","DOIUrl":"10.3347/kjp.2022.60.3.187","url":null,"abstract":"<p><p>The positive rate of Clonorchis sinensis is the highest among intestinal parasites in the Republic of Korea (Korea). More than 1.2 million people were at risk of C. sinensis infection in Korea in 2012. An intensive control program is being implemented for residents of the 5 major river basins to reduce helminthic infections, including C. sinensis infection. This study evaluated the continuous intensive control program for parasitic diseases including clonorchiasis in areas near the 5 major river basins in Korea over the past 10 years (2011-2020). A total of 335,020 fecal samples (one sample per resident) prepared by the modified sedimentation technic were microscopically examined. Those who expelled helminth eggs were treated with anthelmintics through local health centers and re-examined 3 months later. The overall positive rate of helminths egg was 7.1%. The annual positive rates were dramatically decreased from 14.4% (2011) to 5.9% (2020). The egg positive rate was highest in C. sinensis (5.3%), followed by heterophyid flukes (1.5%) and Trichuris trichiura (0.2%). The prevalence of C. sinensis was significantly higher in males (7.6%) than in females (3.7%), and the highest in the 50-59 years (7.0%) age group. Our results are beneficial to establish prevention and control policies against helminthiases including clonorchiasis in endemic areas in this country.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"187-193"},"PeriodicalIF":1.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d4/43/kjp-60-3-187.PMC9256286.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Distribution of Gastrointestinal Parasitic Infection in Domestic Pigs in the Republic of Korea: Nationwide Survey from 2020-2021.","authors":"Subin Lee, Badriah Alkathiri, Dongmi Kwak, Sang-Myeong Lee, Wan-Kyu Lee, Jae-Won Byun, Seung-Hun Lee","doi":"10.3347/kjp.2022.60.3.207","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.207","url":null,"abstract":"<p><p>This study aimed to examine the distribution of gastrointestinal parasitic infections in domestic pigs in the Republic of Korea. From May 2020 to October 2021, 364 pig fecal samples were collected from 75 farms in 7 Provinces and microscopically examined. A total of 170 (46.7%) pigs were infected with at least one of the following parasites: Balantioides coli, strongyles, Ascaris suum, Trichuris suis, and coccidia. By parasite species, B. coli, strongyles, A. suum, T. suis, and coccidia oocysts or eggs were detected in 144 (39.6%), 24 (6.6%), 14 (3.8%), 4 (1.1%), and 1 (0.3%) samples, respectively. One hundred fifty-four, 15, and 1 cases showed single, double, and triple infections, respectively. Of the swine fecal samples from 75 farms, 69 specimens (92.0%) were infected with 1 or more parasites. All surveyed farms across the country exhibited a positive rate of over 30%, among which the highest positive rate was 65.0% in Chungcheongnam-do, and Jeollabuk-do was followed by 61.9%. Winter showed a statistically lower prevalence than other seasons. This study showed that gastrointestinal parasites are prevalent in pigs in Korea, although the diversity of parasites is low.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"207-211"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/a5/kjp-60-3-207.PMC9256285.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gustavo Ponce-Garcia, Olga K Villanueva-Segura, Karina Garza-Elizondo, Heriberto M Villegas-Ramirez, Ildefonso Fernandez-Salas, Iram P Rodriguez-Sanchez, Felipe Dzul-Manzanilla, Adriana E Flores-Suarez
{"title":"Determination of Knockdown Resistance (kdr) Allele Frequencies (T929I mutation) in Head Louse Populations from Mexico, Canada, and Peru.","authors":"Gustavo Ponce-Garcia, Olga K Villanueva-Segura, Karina Garza-Elizondo, Heriberto M Villegas-Ramirez, Ildefonso Fernandez-Salas, Iram P Rodriguez-Sanchez, Felipe Dzul-Manzanilla, Adriana E Flores-Suarez","doi":"10.3347/kjp.2022.60.3.217","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.217","url":null,"abstract":"<p><p>The head louse Pediculus humanus capitis (De Geer) is a hematophagous ectoparasite that inhabits the human scalp. The infestations are asymptomatic; however, skin irritation from scratching occasionally may cause secondary bacterial infections. The present study determined the presence and frequency of the knockdown resistance (kdr) mutation T929I in 245 head lice collected from Mexico, Peru, and Canada. Head lice were collected manually using a comb in the private head lice control clinic. Allele mutation at T9291 was present in 100% of the total sampled populations (245 lice) examined. In addition, 4.89% of the lice were homozygous susceptible, whereas 6.93% heterozygous and 88.16% homozygous were resistant, respectively. This represents the second report in Mexico and Quebec and fist in Lima.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"217-221"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/93/kjp-60-3-217.PMC9256284.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
{"title":"Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica.","authors":"Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin","doi":"10.3347/kjp.2022.60.3.155","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.155","url":null,"abstract":"<p><p>All living organisms are destined to die. Cells, the core of those living creatures, move toward the irresistible direction of death. The question of how to die is critical and is very interesting. There are various types of death in life, including natural death, accidental death, questionable death, suicide, and homicide. The mechanisms and molecules involved in cell death also differ depending on the type of death. The dysenteric amoeba, E. histolytica, designated by the German zoologist Fritz Schaudinn in 1903, has the meaning of tissue lysis; i.e., tissue destroying, in its name. It was initially thought that the amoebae lyse tissue very quickly leading to cell death called necrosis. However, advances in measuring cell death have allowed us to more clearly investigate the various forms of cell death induced by amoeba. Increasing evidence has shown that E. histolytica can cause host cell death through induction of various intracellular signaling pathways. Understanding of the mechanisms and signaling molecules involved in host cell death induced by amoeba can provide new insights on the tissue pathology and parasitism in human amoebiasis. In this review, we emphasized on the signaling role of NADPH oxidases in reactive oxygen species (ROS)-dependent cell death by pathogenic E. histolytica.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"155-161"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/56/56/kjp-60-3-155.PMC9256287.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40466253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guiehdani Villalobos, Fabiola Sanchez-Aguillon, Marcia Veronica Carmona-Maldonado, Nelly Raquel Gonzalez-Arenas, Eduardo Lopez-Escamilla, Rigoberto Hernandez-Castro, Mirza Romero-Valdovinos, Williams Arony Martinez-Flores, Juan Pablo Ramirez-Hinojosa, Pablo Maravilla, Fernando Martinez-Hernandez
{"title":"Unexpected Presence of Blastocystis Subtype 1-3 DNA in Human Vaginal and Sperm Samples Coinfected with Trichomonas vaginalis.","authors":"Guiehdani Villalobos, Fabiola Sanchez-Aguillon, Marcia Veronica Carmona-Maldonado, Nelly Raquel Gonzalez-Arenas, Eduardo Lopez-Escamilla, Rigoberto Hernandez-Castro, Mirza Romero-Valdovinos, Williams Arony Martinez-Flores, Juan Pablo Ramirez-Hinojosa, Pablo Maravilla, Fernando Martinez-Hernandez","doi":"10.3347/kjp.2022.60.3.195","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.195","url":null,"abstract":"<p><p>There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis. Blastocystis STs were identified in 6 of 28 vaginal swabs (21.4%) and in 3 of 7 sperm samples (42.8%). In both biological samples, STs 1-3 were found; one vaginal sample showed subtype co-infection with ST1 and ST3. High genetic variation was observed in the sequences obtained and no specific clustering in the phylogenetic trees was detected. Most of the haplotypes identified were placed far from the main dispersal centers. Our finding suggested that incorrect cleaning of the genital area or a contamination by combination of anal and vaginal intercourse.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"60 3","pages":"195-200"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/23/kjp-60-3-195.PMC9256282.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young Yil Bahk, Shin-Hyeong Cho, Sookkyung Park, Jeongran Kwon, Hyesu Kan, Miyoung Kim, Byoung-Kuk Na, Sung Jong Hong, Hyung Wook Kwon, Tong-Soo Kim
{"title":"Knowledge, Attitudes and Perceptions Regarding Endemic Vivax Malaria in Inhabitants and Patients in Two Cities of Northern Gyeonggi-do, Korea, 2020.","authors":"Young Yil Bahk, Shin-Hyeong Cho, Sookkyung Park, Jeongran Kwon, Hyesu Kan, Miyoung Kim, Byoung-Kuk Na, Sung Jong Hong, Hyung Wook Kwon, Tong-Soo Kim","doi":"10.3347/kjp.2021.59.6.595","DOIUrl":"https://doi.org/10.3347/kjp.2021.59.6.595","url":null,"abstract":"<p><p>An understanding of the knowledges, attitudes and perceptions of different populations is key for public health policy makers. Here, a survey was performed on knowledge, attitudes, and perceptions about malaria diagnosis, prevention, control, and treatment. The 407 survey participants included both uninfected inhabitants and patients from 2 cities (Gimpo- and Paju-si) of Northern Gyeonggi-do, known as high-risk areas for vivax malaria. We used community-based study design and non-probability sampling method using the primary data. Association between variables were tested using χ2-tests. In general, the information on malaria reported by the participants in this study was unsystematic and included inaccurate details. The knowledge of malaria symptoms, identified as headache, chills and fever, was high, but the surveyed community lacks knowledge of the specific medications used for malaria treatment, with a large number of respondents having no knowledge of any form of medication. Survey questions with high correct answer rates included questions about easy treatment of malaria in Korea, the high daytime activity of malaria-borne mosquitoes, and the infection risk posed by outdoor activities. However, a large portion of the respondents was unable to provide simple medical and biological information about the disease. This study aimed to comprehensively evaluate the knowledge, attitude, and practical behavior of the surveyed community with respect to malaria and the implications reported here could be applicable to other malaria endemic areas in Korea.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"59 6","pages":"595-605"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ec/7d/kjp-59-6-595.PMC8721300.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Lu, Jia-Hui Sun, Li-Li Lu, Jia-Xu Chen, Peng Song, Lin Ai, Yu-Chun Cai, Lan-Hua Li, Shao-Hong Chen
{"title":"Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid.","authors":"Yan Lu, Jia-Hui Sun, Li-Li Lu, Jia-Xu Chen, Peng Song, Lin Ai, Yu-Chun Cai, Lan-Hua Li, Shao-Hong Chen","doi":"10.3347/kjp.2021.59.6.615","DOIUrl":"https://doi.org/10.3347/kjp.2021.59.6.615","url":null,"abstract":"<p><p>Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.</p>","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":"59 6","pages":"615-623"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/07/kjp-59-6-615.PMC8721309.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}