Korean Journal of Parasitology最新文献_第3页

Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells. 刚地弓形虫中国ⅰ基因型Wh6株在猫肠上皮细胞中的培养

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.241

Guihua Zhao, Lixin Zhang, Lisha Dai, Haozhi Xu, Chao Xu, Ting Xiao, Jun Li, Hui Sun, Beibei Zhou, Kun Yin

{"title":"Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells.","authors":"Guihua Zhao, Lixin Zhang, Lisha Dai, Haozhi Xu, Chao Xu, Ting Xiao, Jun Li, Hui Sun, Beibei Zhou, Kun Yin","doi":"10.3347/kjp.2022.60.4.241","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.241","url":null,"abstract":"Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8f/96/kjp-60-4-241.PMC9441452.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An Evaluation of a New Quantitative Point-of Care Diagnostic to Measure Glucose-6-phosphate Dehydrogenase Activity. 葡萄糖-6-磷酸脱氢酶活性的一种新的定量护理点诊断评价。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.281

Young Yil Bahk, Seong Kyu Ahn, Heung Jin Jeon, Byoung-Kuk Na, Sung-Keun Lee, Ho-Joon Shin

{"title":"An Evaluation of a New Quantitative Point-of Care Diagnostic to Measure Glucose-6-phosphate Dehydrogenase Activity.","authors":"Young Yil Bahk, Seong Kyu Ahn, Heung Jin Jeon, Byoung-Kuk Na, Sung-Keun Lee, Ho-Joon Shin","doi":"10.3347/kjp.2022.60.4.281","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.281","url":null,"abstract":"Malaria continues to be one of the most crucial infectious burdens in endemic areas worldwide, as well as for travelers visiting malaria transmission regions. It has been reported that 8-aminoquinolines are effective against the Plasmodium species, particularly primaquine, for anti-hypnozoite therapy in P. vivax malaria. However, primaquine causes acute hemolytic anemia in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Therefore, G6PD deficiency testing should precede hypnozoite elimination with 8-aminoquinoline. Several point-of-care devices have been developed to detect G6PD deficiency. The aim of the present study was to evaluate the performance of a novel, quantitative G6PD diagnostics based on a metagenomic blue fluorescent protein (mBFP). We comparatively evaluated the sensitivity and specificity of the G6PD diagnostic modality with standard methods using 120 human whole blood samples. The G6PD deficiency was spectrophotometrically confirmed. The performance of the G6PD quantitative test kit was compared with that of a licensed control medical device, the G6PD strip. The G6PD quantitative test kit had a sensitivity of 95% (95% confidence interval (CI): 89.3-100%) and a specificity of 100% (95% CI: 94.3-100%). This study shows that the novel diagnostic G6PD quantitative test kit could be a cost-effective and time-efficient, and universally mandated screening tool for G6PD deficiency.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c2/f4/kjp-60-4-281.PMC9441449.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR. 细胞色素氧化酶多重PCR与18S rRNA巢式PCR诊断性能的比较评价。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.295

Preeti Kumari, Swati Sinha, Renuka Gahtori, Afshana Quadiri, Paras Mahale, Deepali Savargaonkar, Veena Pande, Bina Srivastava, Himmat Singh, Anupkumar R Anvikar

{"title":"Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR.","authors":"Preeti Kumari, Swati Sinha, Renuka Gahtori, Afshana Quadiri, Paras Mahale, Deepali Savargaonkar, Veena Pande, Bina Srivastava, Himmat Singh, Anupkumar R Anvikar","doi":"10.3347/kjp.2022.60.4.295","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.295","url":null,"abstract":"Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic tools are required for accurate treatment. Molecular diagnosis of malaria is commonly carried out by nested polymerase chain reaction (PCR) targeting 18S rRNA gene, while this technique involves long turnaround time and multiple steps leading to false positive results. To overcome these drawbacks, we compared highly sensitive cytochrome oxidase gene-based single-step multiplex reaction with 18S rRNA nested PCR. Cytochrome oxidase (cox) genes of P. falciparum (cox-III) and P. vivax (cox-I) were compared with 18S rRNA gene nested PCR and microscopy. Cox gene multiplex PCR was found to be highly specific and sensitive, enhancing the detection limit of mixed infections. Cox gene multiplex PCR showed a sensitivity of 100% and a specificity of 97%. This approach can be used as an alternative diagnostic method as it offers higher diagnostic performance and is amenable to high throughput scaling up for a larger sample size at low cost.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/b0/kjp-60-4-295.PMC9441448.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation. 自由生活的蠕形阿米巴诱发过敏性气道炎症。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.229

Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu

{"title":"Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation.","authors":"Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu","doi":"10.3347/kjp.2022.60.4.229","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.229","url":null,"abstract":"The high percentage of Vermamoeba was found in tap water in Korea. This study investigated whether Vermamoeba induced allergic airway inflammation in mice. We selected 2 free-living amoebas (FLAs) isolated from tap water, which included Korean FLA 5 (KFA5; Vermamoeba vermiformis) and 21 (an homolog of Acanthamoeba lugdunensis KA/E2). We axenically cultured KFA5 and KFA21. We applied approximately 1×106 to mice’s nasal passages 6 times and investigated their pathogenicity. The airway resistance value was significantly increased after KFA5 and KFA21 treatments. The eosinophil recruitment and goblet cell hyperplasia were concomitantly observed in bronchial alveolar lavage (BAL) fluid and lung tissue in mice infected with KFA5 and KFA21. These infections also activated the Th2-related interleukin 25, thymic stromal lymphopoietin, and thymus and activation-regulated chemokines gene expression in mouse lung epithelial cells. The CD4+ interleukin 4+ cell population was increased in the lung, and the secretion of Th2-, Th17-, and Th1-associated cytokines were upregulated during KFA5 and KFA21 infection in the spleen, lung-draining lymph nodes, and BAL fluid. The pathogenicity (allergenicity) of KFA5 and KFA21 might not have drastically changed during the long-term in vitro culture. Our results suggested that Vermamoeba could elicit allergic airway inflammation and may be an airway allergen.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/26/a3/kjp-60-4-229.PMC9441446.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40331759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trichinella Infection Ameliorated Vincristine-Induced Neuroinflammation in Mice. 旋毛虫感染可改善长春新碱诱导的小鼠神经炎症。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.247

Young Rae Jo, Hwan Tae Park, Hak Sun Yu, Hyun-Hee Kong

{"title":"Trichinella Infection Ameliorated Vincristine-Induced Neuroinflammation in Mice.","authors":"Young Rae Jo, Hwan Tae Park, Hak Sun Yu, Hyun-Hee Kong","doi":"10.3347/kjp.2022.60.4.247","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.247","url":null,"abstract":"Vincristine (VCR) is a chemotherapeutic agent widely used in treatment of malignancies. However, VCR has a limitation in use since it commonly causes a painful neuropathy (VCR-induced peripheral neuropathy, VIPN). Inflammatory cytokines secreted by immune cells such as macrophages can exacerbate allodynia and hyperalgesia, because inhibiting the inflammatory response is a treatment target for VIPN. In this study, we investigated whether Trichinella spiralis, a widely studied helminth for its immunomodulatory abilities, can alleviate VCR-induced allodynia. Von Frey test showed that T. spiralis infection improved mechanical allodynia at 10 days after VCR injection. We further observed whether the difference was due to mitigated axon degeneration, but no significant difference between the groups in axonal degeneration in sciatic nerves and intra-epidermal nerve fibers was found. Conversely, we observed that number of infiltrated macrophages was decreased in the sciatic nerves of the T. spiralis infected mice. Moreover, treatment of T. spiralis excretory-secretory products caused peritoneal macrophages to secrete decreased level of IL-1β. This study suggests that T. spiralis can relieve VCR-induced mechanical allodynia by suppressing neuroinflammation and that application of controllable degree of helminth may prove beneficial for VIPN treatment.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/76/20/kjp-60-4-247.PMC9441445.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between Clonorchis sinensis Infection and Cholangiocarcinoma in Korea. 中国支支睾吸虫感染与韩国胆管癌的关系。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.261

Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Dongjae Kim, Yeong Hoon Kim

{"title":"Relationship between Clonorchis sinensis Infection and Cholangiocarcinoma in Korea.","authors":"Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Dongjae Kim, Yeong Hoon Kim","doi":"10.3347/kjp.2022.60.4.261","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.261","url":null,"abstract":"This study provides an overview of the current status of clonorchiasis and cholangiocarcinoma (CCA), and their relationship in Korea during 2012-2020. Data were obtained from the Health Insurance Review & Assessment Service of Korea. Cluster, trend, and correlation analyses were performed. Gyeongsangnam-do and Seoul had the highest average number of cases (1,026 and 4,208) and adjusted rate (306 and 424) for clonorchiasis and CCA, respectively. The most likely clusters (MLC) for clonorchiasis and CCA were Busan/Gyeongsangnam-do/Ulsan/Daegu/Gyeongsangbuk-do (Relative Risk; RR = 4.55, Likelihood Ratio; LLR = 9,131.115) joint cluster and Seoul (RR = 2.29, LLR = 7,602.472), respectively. The MLC for clonorchiasis was in the southeastern part of Korea, while that for CCA was in the southern part. Clonorchiasis showed a decreasing trend in the southeastern districts, while increased in the southwestern districts. Cities in the central region had a decreasing trend, while the western districts had an increasing trend. In most adults (30-59), infection rate of clonorchiasis showed a significant decrease until 2018, while thereafter increased, although not significant. CCA showed a sharply decreasing tendency. The incidence of clonorchiasis and CCA were positively correlated. In general, the correlation was weak (r = 0.39, P < 0.001), but it was strongly positive around the 4 river basins (r = 0.74, P < 0.001). This study might provide an analytic basis for developing an effective system against clonorchiasis and CCA.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/6d/kjp-60-4-261.PMC9441450.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Identification and Phylogenetic Analysis of Laelapin Mite Species (Acari: Mesostigmata: Laelapidae) from China. 中国毛螨的形态鉴定与系统发育分析(蜱螨亚纲:中鞭毛目:毛螨科)。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.273

Huijuan Yang, Zhihua Yang, Wenge Dong

{"title":"Morphological Identification and Phylogenetic Analysis of Laelapin Mite Species (Acari: Mesostigmata: Laelapidae) from China.","authors":"Huijuan Yang, Zhihua Yang, Wenge Dong","doi":"10.3347/kjp.2022.60.4.273","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.273","url":null,"abstract":"Laelapinae mites are involved in transmission of microbial diseases between wildlife and humans, with an impact on public health. In this study, 5 mite members in the subfamily Laelapinae (laelapin mites; LM) were morphologically identified by light microscopy, and the phylogenetic relationship of LM was analyzed in combination with the sequence information of part of the LM cytochrome oxidase subunit I (cox1) gene. The morphological identification revealed that 5 mites belonged to the genera Laelaps and Haemolaelaps, respectively. Sequence analysis showed that the ratio of non-synonymous mutation rate to synonymous mutation rate of LM was less than 1, indicating that the LM cox1 gene had undergone purifying selection. Phylogenetic analysis showed that the Laelapinae is a monophyletic group. The genera Haemolaelaps and Hyperlaelaps did not separated into distinct clades but clustered together with species of the genus Laelaps. Our morphological and molecular analyses to describe the phylogenetic relationships among different genera and species of Laelapinae provide a reference for the improvement and revision of the LM taxonomy system.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/eb/kjp-60-4-273.PMC9441453.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Erratum: Spatiotemporal Clusters and Trend of Trichomonas vaginalis Infection in Korea. 勘误:韩国阴道毛滴虫感染的时空聚集和趋势。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.301

引用次数: 0

Ozobranchus jantseanus (Clitellata: Ozobranchidae) from Reeve's Turtle, Mauremys reevesii: New Annelid Fauna in Korea. 来自Reeve’s Turtle的Ozobranchus jantseanus（Clitellata:Ozobranchidae），Mauremys reevesii：韩国的新安氏动物群。

4区医学

Korean Journal of Parasitology Pub Date : 2022-06-01 Epub Date: 2022-06-30 DOI: 10.3347/kjp.2022.60.3.213

Kyo Soung Koo, Kayoung Yun, Yikweon Jang

引用次数: 1

A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti. 荧光重组酶辅助扩增法检测微小巴贝斯虫。

4区医学

Korean Journal of Parasitology Pub Date : 2022-06-01 Epub Date: 2022-06-30 DOI: 10.3347/kjp.2022.60.3.201

Hong Lin, Song Zhao, Yuying Ye, Lei Shao, Nizhen Jiang, Kun Yang

{"title":"A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti.","authors":"Hong Lin, Song Zhao, Yuying Ye, Lei Shao, Nizhen Jiang, Kun Yang","doi":"10.3347/kjp.2022.60.3.201","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.3.201","url":null,"abstract":"Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/µl B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/80/d9/kjp-60-3-201.PMC9256288.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40560656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2