Korean Journal of Parasitology最新文献_第2页

Antimalarial Efficacy of Aqueous Extract of Strychnos ligustrina and Its Combination with Dihydroartemisinin and Piperaquine Phosphate (DHP) against Plasmodium berghei Infection. 马钱子水提物及其与双氢青蒿素和磷酸哌喹(DHP)联合抗伯氏疟原虫感染的研究。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.339

Umi Cahyaningsih, Siti Sa'diah, Wasrin Syafii, Rita Kartika Sari, Abdul Jafar Maring, Arifin Budiman Nugraha

{"title":"Antimalarial Efficacy of Aqueous Extract of Strychnos ligustrina and Its Combination with Dihydroartemisinin and Piperaquine Phosphate (DHP) against Plasmodium berghei Infection.","authors":"Umi Cahyaningsih, Siti Sa'diah, Wasrin Syafii, Rita Kartika Sari, Abdul Jafar Maring, Arifin Budiman Nugraha","doi":"10.3347/kjp.2022.60.5.339","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.5.339","url":null,"abstract":"The development of drug resistance is one of the most severe concerns of malaria control because it increases the risk of malaria morbidity and death. A new candidate drug with antiplasmodial activity is urgently needed. This study evaluated the efficacy of different dosages of aqueous extract of Strychnos ligustrina combined with dihydroartemisinin and piperaquine phosphate (DHP) against murine Plasmodium berghei infection. The BALB/c mice aged 6-8 weeks were divided into 6 groups, each consisting of 10 mice. The growth inhibition of compounds against P. berghei was monitored by calculating the percentage of parasitemia. The results showed that the mice receiving aqueous extract and combination treatment showed growth inhibition of P. berghei in 74% and 94%, respectively. S. ligustrina extract, which consisted of brucine and strychnine, effectively inhibited the multiplication of P. berghei. The treated mice showed improved hematology profiles, body weight, and temperature, as compared to control mice. Co-treatment with S. ligustrina extract and DHP revealed significant antimalarial and antipyretic effects. Our results provide prospects for further discovery of antimalarial drugs that may show more successful chemotherapeutic treatment.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/d8/kjp-60-5-339.PMC9633154.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801. 重组节虫寡孢子几丁质酶AO-801的生物学特性

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.345

Shasha Gong, Qingling Meng, Jun Qiao, Yunfu Huang, Wenqiang Zhong, Guowu Zhang, Kai Zhang, Ningxing Li, Yunxia Shang, Zhiyuan Li, Xuepeng Cai

{"title":"Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801.","authors":"Shasha Gong, Qingling Meng, Jun Qiao, Yunfu Huang, Wenqiang Zhong, Guowu Zhang, Kai Zhang, Ningxing Li, Yunxia Shang, Zhiyuan Li, Xuepeng Cai","doi":"10.3347/kjp.2022.60.5.345","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.5.345","url":null,"abstract":"Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)8 triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40˚C and pH values between 4-7. Enzyme activity was inhibited by Zn2+, Ca2+, and Fe3+, whereas Mg2+ and K+ potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/aa/kjp-60-5-345.PMC9633153.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spatiotemporal Clusters and Trends of Pneumocystis Pneumonia in Korea. 韩国肺孢子虫肺炎的时空分布和趋势。

IF 1.4 4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.327

Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Sang Haak Lee, Yeong Hoon Kim

{"title":"Spatiotemporal Clusters and Trends of Pneumocystis Pneumonia in Korea.","authors":"Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Sang Haak Lee, Yeong Hoon Kim","doi":"10.3347/kjp.2022.60.5.327","DOIUrl":"10.3347/kjp.2022.60.5.327","url":null,"abstract":"This study determined the recent status and trend of Pneumocystis jirovecii pneumonia (PcP) in the non-human immunodeficiency virus (HIV) (non-HIV-PcP) and HIV (HIV-PcP) infected populations using data from the Health Insurance Review & Assessment Service (HIRA) and the Korea Disease Control and Prevention Agency (KDCA). SaTScan and Joinpoint were used for statistical analyses. Non-HIV-PcP cases showed an upward trend during the study period from 2010 to 2021, with the largest number in 2021 (551 cases). The upward trend was similar until 2020 after adjusting for the population. Seoul had the highest number of cases (1,597) in the non-HIV-PcP group, which was the same after adjusting for the population (162 cases/1,000,000). It was followed by Jeju-do (89 cases/1,000,000). The most likely cluster (MLC) for the non-HIV-PCP group was Seoul (Relative Risk (RR)=4.59, Log Likelihood Ratio (LLR)=825.531), followed by Jeju-do (RR=1.59, LLR=5.431). An upward trend was observed among the non-HIV-PcP group in the Jeju-do/Jeollanam-do/Jeollabuk-do/Gyeongsangnam-do/Busan/Daejeon/Daegu/Ulsan joint cluster (29.02%, LLR=11.638, P<0.001) located in the southern part of Korea. Both women and men in the non-HIV groups showed an overall upward trend of PcP during the study period. Men in the 60-69 age group had the highest annual percentage change (APC 41.8) during 2014-2019. In contrast, the HIV groups showed a falling trend of PcP recently. Men in the 60-69 age group had the most decrease (APC -17.6) during 2018-2021. This study provides an analytic basis for health measures and a nationwide epidemiological surveillance system for the management of PcP.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/10/2a/kjp-60-5-327.PMC9633162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excretory-Secretory Products of Trichomonas vaginalis Cause Apoptosis in Mouse Sperm in Vitro. 阴道毛滴虫的排泄产物引起小鼠精子体外凋亡。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.357

Jihyun Keum, Jaesook Roh, Jae-Sook Ryu, Ki-Young Ryu

引用次数: 0

Hypoxia-Inducible Factor-1 Alpha Stabilization in Human Macrophages during Leishmania major Infection Is Impaired by Parasite Virulence. 缺氧诱导因子-1 α稳定在利什曼原虫感染的人巨噬细胞被寄生虫毒力削弱。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.317

Ali Ben-Cheikh, Aymen Bali, Fatma Z Guerfali, Chiraz Atr, Hanène Attia, Dhafer Laouini

{"title":"Hypoxia-Inducible Factor-1 Alpha Stabilization in Human Macrophages during Leishmania major Infection Is Impaired by Parasite Virulence.","authors":"Ali Ben-Cheikh, Aymen Bali, Fatma Z Guerfali, Chiraz Atr, Hanène Attia, Dhafer Laouini","doi":"10.3347/kjp.2022.60.5.317","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.5.317","url":null,"abstract":"Hypoxia-inducible factor-1 alpha (HIF-1α) is one of the master regulators of immune and metabolic cellular functions. HIF-1α, a transcriptional factor whose activity is closely related to oxygen levels, is a target for understanding infectious disease control. Several studies have demonstrated that HIF-1α plays an important role during the infectious process, while its role in relation to parasite virulence has not been addressed. In this work, we studied the expression levels of HIF-1α and related angiogenic vascular endothelial growth factor A (VEGF-A) in human macrophages infected with promastigotes of hypo- or hyper-virulent Leishmania major human isolates. L. major parasites readily subverted host macrophage functions for their survival and induced local oxygen consumption at the site of infection. In contrast to hypo-virulent parasites that induce high HIF-1α expression levels, hyper-virulent L. major reduced HIF-1α expression in macrophages under normoxic or hypoxic conditions, and consequently impeded the expression of VEGF-A mRNA. HIF-1α may play a key role during control of disease chronicity, severity, or outcome.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d4/d0/kjp-60-5-317.PMC9633161.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cerebral Paragonimiasis Presenting with Dementia. 脑吸虫病表现为痴呆。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.353

Seok Woo Moon, Taeho Kim

引用次数: 0

Alaria alata (Digenea: Diplostomidae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea. 韩国貉原yonoides koreensis的Alaria alata (digeneia: diplostomae)。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.361

Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park

{"title":"Alaria alata (Digenea: Diplostomidae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea.","authors":"Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park","doi":"10.3347/kjp.2022.60.5.361","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.5.361","url":null,"abstract":"We report a species of diplostomid fluke recovered from 3 carcasses of wild Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 107 diplostomid flukes were recovered from the small intestines of Korean raccoon dogs, which were obtained from the Gangwon Wildlife Medical Rescue Center. Worms fixed with 10% neutral formalin were subjected to microscopic observation and those fixed in 70% ethanol were used for molecular genomic analysis. The worm was divided into 2 separate parts, forebody and hindbody, with a total length of 3,020-4,090 (3,855) µm and a width of 1,210-1,770 (1,562) µm. The boat-shaped forebody has a pair of characteristic tentacular appendage, 2 suckers, holdfast organ, and vitelline follicles. The oval to cylindrical hindbody has reproductive organs. The ovary was round or elliptical and located in the anterior of the testes. Two large testes were slightly segmented and tandemly arranged, occupying almost half of hindbody. The short uterus contained a relatively small number of unembryonated eggs sized 130-140×85-96 µm. The partial sequence of 18S rRNA of this fluke was consistent with Alaria alata. Based on the morphological and molecular characteristics, the diplostomid flukes recovered from the small intestine of Korean raccoon dogs were identified as A. alata (Digenea: Diplostomidae).","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/ec/kjp-60-5-361.PMC9633156.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40448257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Characteristics of Fasciola hepatica Isolated from a Korean Patient. 韩国人肝片形吸虫分离株的系统发育特征。

4区医学

Korean Journal of Parasitology Pub Date : 2022-10-01 Epub Date: 2022-10-21 DOI: 10.3347/kjp.2022.60.5.367

Mi Jin Jeong, Jae Kyun Park, Hak Sun Yu

引用次数: 1

Mapping of the Complement C9 Binding Region on Clonorchis sinensis Paramyosin. 华支睾吸虫副肌球蛋白补体C9结合区的定位。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.255

Jung-Mi Kang, Hương Giang Lê, Tuấn Cường Võ, Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na

{"title":"Mapping of the Complement C9 Binding Region on Clonorchis sinensis Paramyosin.","authors":"Jung-Mi Kang, Hương Giang Lê, Tuấn Cường Võ, Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na","doi":"10.3347/kjp.2022.60.4.255","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.255","url":null,"abstract":"Heliminthic paramyosin is a multifunctional protein that not only acts as a structural protein in muscle layers but as an immune-modulatory molecule interacting with the host immune system. Previously, we found that paramyosin from Clonorchis sinensis (CsPmy) is bound to human complement C9 protein (C9). To analyze the C9 binding region on CsPmy, overlapping recombinant fragments of CsPmy were produced and their binding activity to human C9 was investigated. The fragmental expression of CsPmy and C9 binding assays revealed that the C9 binding region was located at the C-terminus of CsPmy. Further analysis of the C-terminus of CsPmy to narrow the C9 binding region on CsPmy indicated that the region flanking731Leu-780 Leu was a potent C9 binding region. The CsPmy fragments corresponding to the region effectively inhibited human C9 polymerization. These results provide a precise molecular basis for CsPmy as a potent immunomodulator to evade host immune defenses by inhibiting complement attack.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/a7/kjp-60-4-255.PMC9441447.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Detection and Genetic Diversity of Blastocystis in Korean Dogs. 韩国犬囊虫的分子检测及遗传多样性。

4区医学

Korean Journal of Parasitology Pub Date : 2022-08-01 Epub Date: 2022-08-24 DOI: 10.3347/kjp.2022.60.4.289

Sangsu Suh, Haeseung Lee, Min-Goo Seo, Kyoo-Tae Kim, Kyung-Yeon Eo, Young-Sam Kwon, Sang-Joon Park, Oh-Deog Kwon, Tae-Hwan Kim, Dongmi Kwak

{"title":"Molecular Detection and Genetic Diversity of Blastocystis in Korean Dogs.","authors":"Sangsu Suh, Haeseung Lee, Min-Goo Seo, Kyoo-Tae Kim, Kyung-Yeon Eo, Young-Sam Kwon, Sang-Joon Park, Oh-Deog Kwon, Tae-Hwan Kim, Dongmi Kwak","doi":"10.3347/kjp.2022.60.4.289","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.4.289","url":null,"abstract":"Blastocystis is a genus of unicellular heterokont parasites belonging to a group of organisms known as Stramenopiles, which includes algae, diatoms, and water molds. Blastocystis includes several species that habitat in the gastrointestinal tracts of organisms as diverse as humans, farm animals, birds, rodents, reptiles, amphibians, fish, and cockroaches. It is important to public health and distributed globally, but its prevalence in dogs in Korea has not been reported to date. Here, we collected 787 canine fecal samples and assessed Blastocystis infection by age, sex, region, season, and diarrhea symptoms. We determined Blastocystis subtypes using phylogenetic analyses based on 18S rRNA gene sequences. We identified, 10 Blastocystis positive samples (1.3%). A higher proportion of infected dogs was asymptomatic; however, infection rates did not significantly differ according to region, age, sex, and season. Phylogenetic analysis showed that the Blastocystis sp. identified belonged to 4 subtypes (STs), ST1, ST5, ST10, and ST14, thus revealed the genetic diversity of Blastocystis sp. in dogs Korean. This is first report on the presence of Blastocystis sp. in dogs Korean. This study revealed a lower infection rate than expected and differed from previous studies in STs. Further studies are warranted to observe the national infection status of Blastocystis in dogs and the genetic characteristics of this genus.","PeriodicalId":49938,"journal":{"name":"Korean Journal of Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7f/2b/kjp-60-4-289.PMC9441454.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40332689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2