Libyan Journal of Medicine最新文献

{"title":"Whole-genome sequencing of SARS-COV-2 showed wide spread of B.1.525 in February 2021 in Libya.","authors":"Inas M Alhudiri,&nbsp;Ahmad M Ramadan,&nbsp;Khaled M Ibrahim,&nbsp;Adel Abdalla,&nbsp;Mouna Eljilani,&nbsp;Mohamed Ali Salem,&nbsp;Hajer Mohamed Elgheriani,&nbsp;Salah Edin El Meshri,&nbsp;Adam Elzagheid","doi":"10.1080/19932820.2021.2001210","DOIUrl":"https://doi.org/10.1080/19932820.2021.2001210","url":null,"abstract":"<p><p>Alpha (B.1.1.7) SARS-COV-2 variant was detected in September 2020 in minks and humans in Denmark and UK. This variant has several mutations in the spike region (S) which could increase the transmissibility of the virus 43-90% over previously circulating variants. The National Center for Disease Control (NCDC) announced on 24 February 2021 a 25% frequency of B.1.1.7 strain in Libya using a reverse-transcriptase quantitative PCR assay. This assay relies on the specific identification of the H69-V70 deletion in S gene which causes its failure of amplification (SGTF). This deletion is not specific for B.1.1.7, but is also characteristic of two other SARS-COV-2 variants. This study aimed to estimate the frequency of B.1.1.7 and identify other variants circulating in Libya in February 2021. We performed whole genome sequencing of 67 positive SARS-COV-2 samples collected on 25 February 2021 in Libya which were also tested by RT-qPCR for SGTF. Our results showed that 55% of samples had mutations specific to B.1.525 strain and only ~3% of samples belonged to B.1.1.7. These findings suggested that B.1.525 was spreading widely in Libya. The use of such RT-qPCR assay, although useful to track some variants, cannot discriminate between variants with H69-V70 deletion. RT-qPCR assays could be multiplexed to identify multiple variants and screen samples prior to sequencing. We emphasize on the need for providing whole-genome sequencing to the main COVID-19 diagnostic laboratories in Libya as well as establishing international collaboration for building capacity and advancing research in this time of the pandemic.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1c/23/ZLJM_16_2001210.PMC8583741.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39874247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of direct-acting antiviral drugs against hepatitis C virus: predictive factors of response to the treatment.","authors":"María E Cárdaba-García, Encarnación Abad-Lecha, Miguel Á Calleja-Hernández","doi":"10.1080/19932820.2021.1949797","DOIUrl":"10.1080/19932820.2021.1949797","url":null,"abstract":"<p><p><b>Background/Aims.</b> Despite the high efficacy and safety of direct-acting antivirals against hepatitis C virus shown in clinical trials, treatment failures continue to occur. Our aim was to establish the effectiveness of these drugs in routine clinical practice, as well as to determine factors that could influence the response to the treatment.<b>Matherials/methods.</b> Single-center, observational, retrospective study. Clinical, virological and pharmacotherapeutic variables were registered at baseline. Adverse drug reactions that occurred were recorded until week 24 of follow-up. Achievement of sustained virologic response was also recorded. Univariate and multivariate analysis were done to determine factors of response.<b>Results.</b> A total of 333 treatment regimens corresponding to 330 different patients were evaluated. Sustained virologic response rate was 94.6% [95%CI: 91.6-96.6%]. 67.9% of the patients experienced adverse drugs reactions (92.2% were grade 1). The univariate analysis identified a higher baseline of platelets, albumin and total cholesterol as predictive factors of sustained virologic response (p < 0.05). Presence of diabetes and complications related to liver disease (splenomegaly, portal hypertension, portal hypertensive gastropathy), body mass index ≥30, greater liver fibrosis, receiving simeprevir and higher baseline levels of glucose, aspartate-aminotransferase, alanine-aminotransferase and alkaline-phosphatase, have been identified as predictive factors of non-response (p < 0.05). The multivariate analysis detected the following independent factors of non-response: body mass index ≥30 and presence of complications related to liver disease.<b>Conclusion.</b> The effectiveness and safety of direct-acting antivirals against hepatitis C virus have been maintained in routine clinical practice. Further research on predictive factors of response is required in order to develop more reliable and reproducible predictive models.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/fb/ZLJM_16_1949797.PMC8317931.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39221899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin with lycopene modulates enzymic antioxidant genes pathway in isoproterenol cardiotoxicity in rats.","authors":"Lijuan Chen, Xiaoli Wu, Weiwei Wang, Xia Wang, Jianhua Ma","doi":"10.1080/19932820.2021.1943924","DOIUrl":"10.1080/19932820.2021.1943924","url":null,"abstract":"<p><p>Quercetin (QN) is a naturally occurring phenolic compound found largely in vegetables and fruits. Lycopene (LY) is yet another natural phytocompound, found abundantly in red-colored fruits and vegetables. Both have been reported to have beneficial activities in humans. In this study, we document <i>in vivo</i> experimental model for isoproterenol (ISO) cardiac injury toxicity in Sprague-Dawley (SD) rats and treatment with a combined optimized concentration of quercetin and lycopene (QL). Male SD rats of different groups were treated with QL (80 mg/kg QN and 3 mg/kg LY together <i>p.o</i>.) for 10 days with ISO administration (100 mg/kg <i>i.p</i>.) on days 7 and 8. After experimental period, CK-MB, TROP, AST, ALT, LDH, GST, GPx, CAT, SOD, Vit.E, Vit. C, GSH, GSSG and MDA were estimated. SD rats administered with ISO showed an obvious rise in the serum marker enzyme levels and tissue oxidative stress markers (MDA and GSSG). Furthermore, marked reductions in the body weight and increases enzymic and non-enzymic antioxidant levels were noticed. Histological features of the heart also indicated a disruption in the cardiac myofibrils structure of ISO-intoxicated rats. Also, quantitative PCR analysis revealed an involvement of antioxidant and related pathway genes such as Nrf2, HO-1, NQO1, GSTµ, SOD1, SOD2, CAT and BCl-2 genes. QL pretreatment prevented all these adverse effects of ISO cardiotoxicity and significantly reduced the myocardial damage. Decrease in oxidative stress was observed, possibly through alterations in the expression levels of enzymic antioxidant genes (GSTµ, SOD1, SOD2 and CAT). In general, QL exert a strong protective effect through the modulations in enzymic antioxidant activity and associated molecular pathways-regulating effect in cardiovascular disease.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/01/f5/ZLJM_16_1943924.PMC8218693.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39251272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficiency of distress thermometer in the determination of supporting needs for cancer inpatients.","authors":"Abdullah Al-Shaaobi,&nbsp;Murad Alahdal,&nbsp;Shiying Yu,&nbsp;Hongming Pan","doi":"10.1080/19932820.2021.1957199","DOIUrl":"https://doi.org/10.1080/19932820.2021.1957199","url":null,"abstract":"<p><p>Psychological distress scale is highly recommended for cancer patients' care. Several psychological scales have been implemented in cancer outpatient clinics. However, the use of the psychological distress scale, particularly distress thermometer (DT), in the inpatient has not been reported. In this study, we report the efficacy of DT in the determination of cancer inpatients' supporting needs.A total of 170 inpatients diagnosed with cancer have been enrolled in this study. Only 132 patients matched the inclusion criteria, while other cases were excluded because of other diseases associated with cancer. The standardized problem list (PL) and Hospital Anxiety and Depression Scale (HADS) were implemented in comparison with DT. Then, the cut-off score of DT was performed to identify clinically significant differences.The analysis of the receiver operating characteristic (ROC) curve revealed that a DT cut-off score of 4 displayed 0.76 under the ROC curve. Sensitivity showed 0.86 sensitivity for cut-off score 4 and a specificity of 0.56 relative to the HADS cut-off score (≥15). DT scores were found independent of medical variables such as cancer type and stage, recurrence, or metastasis. Clinical ECOG-SP showed a significant association with the DT cut-off score (P ≤ 0.05). Regarding PL, patients with scores above DT cut-off were suffering 21 of 40 problems in all categories. Furthermore, patients that scored above the DT cut-off significantly showed an association with high support needs.DT scale showed significant performance in the evaluation of psychological distress among cancer inpatients through the efficient determination of their support needs.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/e1/ZLJM_16_1957199.PMC8344234.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39276579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Associated with Mental Health Outcomes: Results from a Tertiary Referral Hospital in Lebanon during the COVID-19 Pandemic.","authors":"Fatima Msheik El Khoury,&nbsp;Farid Talih,&nbsp;Mohamad F El Khatib,&nbsp;Nadine Abi Younes,&nbsp;Midhat Siddik,&nbsp;Sahar Siddik-Sayyid","doi":"10.1080/19932820.2021.1901438","DOIUrl":"https://doi.org/10.1080/19932820.2021.1901438","url":null,"abstract":"<p><p>Coronavirus Disease (COVID-19) has caused global mental health impacts, and healthcare workers (HCWs) face an increased risk of exposure to the disease when compared to the general population. This study aimed to assess factors associated with mental health among Lebanese HCWs six months after the start of the COVID-19 pandemic. A cross-sectional study was conducted among HCWs at a tertiary hospital, in Lebanon between June and July 2020. The survey included data on demographics, exposure to COVID-19, preparedness to COVID-19 outbreak, risk perceptions of COVID-19, and mental health dimensions. Chi-squared and Fisher's exact tests were used to understand the association among these variables. One hundred and ninety-three of 1,600 Lebanese HCWs participated. More than 80% reported high preparedness levels towards the COVID-19 outbreak, 69% believed that their job was putting them at risk, and 70% altruistically accepted these risks. Anxiety and depression symptomatology were present in 24% and 23% of HCWs; who were more likely to feel more stress at work (83% vs 60%; <i>p</i> = 0.004; 82% vs 61%; <i>p</i> = 0.01, respectively), feel afraid of falling ill (72% vs 55%; <i>p</i> = 0.03; 77% vs 54%; <i>p</i> = 0.01, respectively), fear death (21% vs 7%; <i>p</i> = 0.01; 25% vs 6%; <i>p</i> ≤ 0.001, respectively), and believed that people avoided their families (39% vs 21%; <i>p</i> = 0.01; 35% vs 65%; <i>p</i> = 0.02, respectively). HCWs who reported signs of depression were less likely to altruistically accept the risks of caring for COVID-19 patients, compared to those who did not (57% vs 74%; <i>p = </i>0.03). This study aimed to detect factors associated with mental health among Lebanese HCWs during the COVID-19 pandemic. Findings suggested that altruistic acceptance of COVID-19 risks is higher among HCWs with positive exposure history to COVID-19 and those with less depressive symptomatology.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19932820.2021.1901438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25562141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corylin suppresses metastasis of breast cancer cells by modulating miR-34c/LINC00963 target.","authors":"Shourong Liu,&nbsp;Li Wang,&nbsp;Run Zhang","doi":"10.1080/19932820.2021.1883224","DOIUrl":"https://doi.org/10.1080/19932820.2021.1883224","url":null,"abstract":"<p><p>Breast cancer is one of the cancers leading to most death cases among women and metastasis is the major cause of breast cancer mortality. In this study, Corylin, the flavonoid compound which is extracted and purified from <i>Psoralea corylifolia</i> L., the effect on breast cancer metastasis was investigated. Corylin showed inhibitory effect on migration and invasion abilities of breast cancer cells. Meanwhile, the epithelial-mesenchymal transition was also regulated by corylin. The long non-coding RNA LINC00963 was found to have a significantly high expression level in breast cancer while it can be down-regulated by corylin. In addition, both wound-healing assay and transwell assay showed that LINC00963 induced breast cancer cells metastasis. MiR-34c was increased by corylin treatment depending on p53, and it was firstly identified that the LINC00963 was a direct target of miR-34c. Corylin was verified here that it prohibited MCF-7 migration and invasion depending on miR-34c/LINC00963 target. In conclusion, corylin suppresses metastasis of breast cancer cells via increasing miR-34c expression, which was dependent on p53. LINC00963 was a direct target of miR-34c and the target axis was necessary for corylin function. Therefore, corylin is a promising drug candidate and LINC00963 can be seen as a promising target in breast cancer treatment.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19932820.2021.1883224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25342157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumour activity of Annona muricata L. leaf methanol extracts against Ehrlich Ascites Carcinoma and Dalton's Lymphoma Ascites mediated tumours in Swiss albino mice.","authors":"Aditi Venkatesh Naik,&nbsp;Shanti N Dessai,&nbsp;Krishnan Sellappan","doi":"10.1080/19932820.2020.1846862","DOIUrl":"https://doi.org/10.1080/19932820.2020.1846862","url":null,"abstract":"<p><p>The use of plants as a source of sedative or treatment for cancer is reasonably widespread worldwide. <i>Annona muricata</i> Linn exhibits a vast array of medicinal and ethno-pharmaceutical benefits, attributed by different plant parts. The activity of this plant is regarded to the bio-production of secondary metabolites like alkaloids, phenols, flavonoids, and most unique group of compounds, namely, annonaceous acetogenins. Whilst this plant is gaining popularity as an anticancer treating plant, this study was undertaken to verify the plausible anticancer effect of leaf methanol extracts of <i>A. muricata</i> (LEAM). Acute toxicity study was carried to obtain safe dose in mice models using haematological, biochemical, and histological evaluations in Swiss albino mice. <i>In-vitro</i> cytotoxicity towards Dalton's Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) cell lines were determined by trypan blue exclusion method. <i>In-vivo</i> antitumour activity of LEAM (100, 200, and 500mg/kg b.wt.) was evaluated using DLA induced solid carcinoma and EAC induced ascites carcinoma models and its comparison with standard drug Cisplatin. Acute toxicity studies did not exhibit significant variations in treated mice suggesting diminutive side effects of LEAM. Statistical analysis revealed the IC₅₀ values for DLA and EAC cell lines as 85.56 ± 5.28 and 68.07 ± 7.39 µg/mL, respectively, indicating better cytotoxic activity against EAC than DLA cells. LEAM decreased the tumour burden in dose-dependent manner. In comparison, with different concentrations tested, treatment with LEAM (200 mg/kg b.wt. and 500 mg/kg b.wt.) significantly reduced the solid tumour volume development by 58.11% and 65.70%, respectively. While lifespan was prolonged up to 51.43% in 500 mg/kg b.wt. LEAM treated ascites tumour-induced mice. This study thus indicates that LEAM possesses potent cytotoxic and antineoplastic activity and calls for more methodical safety assessments and other end-points of anti-tumourigenesis. <b>Abbreviations</b>: <b>LEAM</b>: Leaf methanol extract of <i>Annona</i> muricata; <b>DLA</b>: Dalton's Lymphoma Ascites; <b>EAC</b>: Ehrlich Ascites Carcinoma; <b>IC₅₀</b> : Half maximal inhibitory concentration; <b>CPCSEA</b>: Committee for the Purpose of Control Supervision of Experiments on Animal; <b>IAEC</b>: Institutional Animal Ethics Committee; <b>ARRIVE</b>: Animal Research: Reporting <i>In-vivo</i> Experiments; <b>DMSO</b>: Dimethyl sulphoxide; <b>LD₅₀</b> : Lethal Dose, 50%; <b>SD</b>: Standard Deviation; <b>Hb</b>: Haemoglobin; <b>RBC</b>: Red blood cells; <b>WBC</b>: White blood cells; <b>HCT</b>: Hematocrit; <b>MCV</b>: Mean cell volume; <b>MCH</b>: Mean cell haemoglobin; <b>MCHC</b>: Mean cell haemoglobin concentration; <b>SALP</b>: Serum alkaline phosphatase; <b>SGPT</b>: Serum glutamic pyruvic transaminase; <b>SGOT</b>: Serum glutamic oxaloacetic transaminase; <b>ATP</b>: Adenosine triphosphate; <b>EGFR</b","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19932820.2020.1846862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38765694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Travel during COVID-19 pandemic in Libya: reasons of travel, disease importation and travel regulations.","authors":"Ahmad M Ramadan,&nbsp;Khaled M Ibrahim,&nbsp;Saadeddin Mohammed Belaid,&nbsp;Mohamed Salem Abusanina,&nbsp;Mohammed Ben Elfghi,&nbsp;Elmundr Abughnia,&nbsp;Ahmed Elkikkli,&nbsp;Inas M Alhudiri,&nbsp;Adam Elzagheid","doi":"10.1080/19932820.2021.1994740","DOIUrl":"10.1080/19932820.2021.1994740","url":null,"abstract":"<p><p>Restriction of mobility between countries is an important regulatory measure to combat pandemics such as the coronavirus disease 2019 (COVID-19). Currently, PCR testing is required to enter the Libyan borders. However, no post-travel quarantine is employed. In this report, we briefly discuss travel regulations in Libya during the COVID-19 pandemic and disease importation by travelers. The results showed that almost half of the sample travel because of health care and therapy reasons. Tunisia was the most visited destination mainly for trading and business and receiving healthcare. Importantly, 13% of asymptomatic travelers were SARS-CoV-2 positive. Issues regarding repeated testing among very frequent travelers and variant importation needs to be addressed in a more efficient manner.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/da/ZLJM_16_1994740.PMC8547827.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of levosimendan combined with recombinant human brain natriuretic peptide on diuretic resistance.","authors":"Shen Xiangli,&nbsp;Li Lan,&nbsp;Zu Libiya,&nbsp;Ma Jun,&nbsp;Jiang Shubin","doi":"10.1080/19932820.2021.1973762","DOIUrl":"https://doi.org/10.1080/19932820.2021.1973762","url":null,"abstract":"<p><p>Levosimendan is a calcium sensitizer used for managing heart failure (HF) because of its inotropic and vasodilatory effects. As many patients do not respond to levosimendan as a monotherapy, it may be necessary to combine it with other diuretic agents such as recombinant human brain natriuretic peptide (rhBNc P). The aim of this study was to investigate efficacy of levosimendan when combined with rhBNP in patients with diuretic resistance and low ejection fraction (EF) rate.The study included HF patients with diuretic resistance and low EF. Before grouping, patients with a 24-hour urine volume of <0.5 mL/kg/h were administered with furosemide injection. Treatment group was administered levosimendan injection based on the original diuretic and rhBNP.One hundred twenty-eight patients were included, with 64 patients each in the control and treatment arms. 24-hour urine volume of the treatment group was significantly higher than that of the control group. Moreover, dyspnea score of the treatment group significantly improved compared with control group. In the treatment group, 12.5% of patients had no significant changes in the urine volume, weight, and dyspnea score before and after the treatment, indicating poor curative effect of the treatment, whereas in the control group, 23.4% of patients had poor curative effect (<i>P</i> < .05). No significant change was observed in the systolic blood pressure, heart rate, and serum creatinine level before and after treatment in both groups.Levosimendan in combination with rhBNP can effectively relieve diuretic resistance, reduce body weight, improve dyspnea, and ensure safety in the treatment process.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/15/ZLJM_16_1973762.PMC8439246.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39393047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistome and microbial profiling of pediatric patient's gut infected with multidrug-resistant diarrhoeagenic <i>Enterobacteriaceae</i> using next-generation sequencing; the first study from Pakistan.","authors":"Ome Kalsoom Afridi, Johar Ali, Jeong Ho Chang","doi":"10.1080/19932820.2021.1915615","DOIUrl":"10.1080/19932820.2021.1915615","url":null,"abstract":"<p><p>A high prevalence of multidrug-resistant (MDR) pathogens has been reported in adult and pediatric populations of Pakistan. However, data describing the effect of MDR microbes on the gut microbiota is scarce. We designed a cross-sectional pediatric study to investigate the effect of MDR microbes' infection on the gut microbiome and its resistome of children using high-throughput next-generation sequencing (NGS). A cross-sectional study was conducted at a tertiary health care hospital in Peshawar Pakistan, between 5 September 2019 to 15 February 2020. Pediatric patients with acute gastroenteritis (n = 200) were enrolled. All the enrolled pediatric patients underwent initial antimicrobial resistance (AMR) screening using the disk diffusion method. Children with MDR infections were identified and selected for gut microbiome and its resistome profiling using NGS. Out of 200 enrolled pediatric patients, 80 (40%) were found infected with MDR diarrheagenic <i>Enterobacteriaceae</i> consisting of 50 (62.5%) infections caused by extended-spectrum beta-lactamase (ESBL) producing <i>E. coli</i> while 30 (37.5%) by MDR <i>Enterobacter</i> specie. A total of 63 and 17 antibiotic-resistant genes (ARGs) conferring resistance to 7 and 5 classes of antibiotics were identified in the resistomes of MDR diarrheagenic <i>Enterobacteriaceae</i> infected and healthy children, respectively. NGS-based gut microbial profiling of MDR <i>Enterobacter spp</i>., ESBL producing <i>E. coli</i> infected pediatric patients and healthy controls revealed the predominance of <i>Proteobacteria</i> and <i>Actinobacteria</i>, respectively. An increased abundance of several pathogenic gram-negative bacteria namely <i>E. coli, Enterobacter cloacae</i>, and <i>Salmonella enterica</i> was observed in the gut microbiota of children infected with MDR bacterial infections than that of the healthy controls. This work indicates that children with MDR infections have reduced microbial diversity and enriched ARGs than healthy controls. The emergence of MDR bacterial strains and their association with gut dysbiosis needs immediate attention to regulate antibiotics usage in Pakistani children.</p>","PeriodicalId":49910,"journal":{"name":"Libyan Journal of Medicine","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19932820.2021.1915615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38890657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}