Mechanisms of Development最新文献_第4页

The gene regulatory control of sea urchin gastrulation 海胆原肠形成的基因调控

IF 2.6

Mechanisms of Development Pub Date : 2020-06-01 DOI: 10.1016/j.mod.2020.103599

Charles A. Ettensohn

引用次数: 13

Simulations of sea urchin early development delineate the role of oriented cell division in the morula-to-blastula transition 海胆早期发育的模拟描述了定向细胞分裂在桑葚胚向囊胚过渡中的作用

IF 2.6

Mechanisms of Development Pub Date : 2020-06-01 DOI: 10.1016/j.mod.2020.103606

Lawrence Bodenstein

引用次数: 6

MicroRNA-1-3p enhances osteoblast differentiation of MC3T3-E1 cells by interacting with hypoxia-inducible factor 1 α inhibitor (HIF1AN) MicroRNA-1-3p通过与缺氧诱导因子1 α抑制剂(HIF1AN)相互作用促进MC3T3-E1细胞成骨分化

IF 2.6

Mechanisms of Development Pub Date : 2020-06-01 DOI: 10.1016/j.mod.2020.103613

Long Zhou , Min Qiu , Lei Yang , Liyu Yang , Yiqi Zhang , Shuai Mu , Hanyi Song

{"title":"MicroRNA-1-3p enhances osteoblast differentiation of MC3T3-E1 cells by interacting with hypoxia-inducible factor 1 α inhibitor (HIF1AN)","authors":"Long Zhou , Min Qiu , Lei Yang , Liyu Yang , Yiqi Zhang , Shuai Mu , Hanyi Song","doi":"10.1016/j.mod.2020.103613","DOIUrl":"10.1016/j.mod.2020.103613","url":null,"abstract":"<div>Studies have proved that miRNAs participate in the regulation of osteoblast differentiation (OD), and abnormal expression of miRNAs is related with various states of OD. In this study, we investigated the role of miRNA-1-3p in OD using MC3T3-E1 cells. BMP2 is used to induce OD of MC3T3-E1 cells. MiRNA-1-3p mimics or miRNA-1-3p inhibitor was transfected to MC3T3-E1 cells with BMP2. The expression levels of miRNA-1-3p were determined by qRT-PCR. The expression of Runx2, OSX, OPN, and OCN was detected by Western blotting. ALP assay was performed to measure alkaline phosphatase activity. Calcium nodules were evaluated by alizarin red staining. Over-expression of hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) was performed and miRNA-1-3p rescue experiments were carried out. Over-expression of miRNA-1-3p promoted osteogenic differentiations and calcifications, as demonstrated by increased ALP, calcification and osteogenic markers. Knock-down of miRNA-1-3p generated the opposite results. HIF1AN was identified to be directly targeted by miRNA-1-3p. Over-expression of HIF1AN suppressed OD and calcifications, and miRNA-1-3p reversed the effect. Our results demonstrated that miRNA-1-3p could enhance OD of MC3T3-E1 cells through interacting with HIF1AN, which might be employed as therapeutic applications for bone formation and regeneration.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"162 ","pages":"Article 103613"},"PeriodicalIF":2.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2020.103613","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37917184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Binary fission in Trichoplax is orthogonal to the subsequent division plane 盘虫的二元裂变与随后的分裂平面是正交的

IF 2.6

Mechanisms of Development Pub Date : 2020-06-01 DOI: 10.1016/j.mod.2020.103608

Jorge Zuccolotto-Arellano, Rodrigo Cuervo-González

{"title":"Binary fission in Trichoplax is orthogonal to the subsequent division plane","authors":"Jorge Zuccolotto-Arellano, Rodrigo Cuervo-González","doi":"10.1016/j.mod.2020.103608","DOIUrl":"10.1016/j.mod.2020.103608","url":null,"abstract":"<div>Asexual reproduction in Trichoplax occurs mainly by binary fission and occasionally by the budding of epithelial spheres called “swarmers”. The process that leads to binary fission and the mechanisms involved in this segregation are practically unknown. Trichoplax lacks a defined shape, presenting a constantly changing outline due to its continuous movements and body contractions. For this reason, and due to the absence of anatomical references, it has been classified as an asymmetric organism. Here, we report that a transient wound is formed in the marginal epithelium of the two new individuals produced by binary fission. By tracking the location of this epithelial wound, we can determine that successive dichotomous divisions are orthogonal to the previous division. We also found that LiCl paralyzes the cilia beating movement and body contractions and causes the placozoans to become circular in shape. This effect, as well as a stereotypic body folding behavior observed in detached placozoans and cell labeling experiments of the upper epithelium, indicate a cylindrical body symmetry for Placozoa.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"162 ","pages":"Article 103608"},"PeriodicalIF":2.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2020.103608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37917185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Protein kinases: master regulators of neuritogenesis and therapeutic targets for axon regeneration. 蛋白激酶：神经发生的主调节器和轴突再生的治疗靶点。

IF 8

Mechanisms of Development Pub Date : 2020-04-01 Epub Date: 2019-10-28 DOI: 10.1007/s00018-019-03336-6

Sarah A Bennison, Sara M Blazejewski, Trevor H Smith, Kazuhito Toyo-Oka

{"title":"Protein kinases: master regulators of neuritogenesis and therapeutic targets for axon regeneration.","authors":"Sarah A Bennison, Sara M Blazejewski, Trevor H Smith, Kazuhito Toyo-Oka","doi":"10.1007/s00018-019-03336-6","DOIUrl":"10.1007/s00018-019-03336-6","url":null,"abstract":"Proper neurite formation is essential for appropriate neuronal morphology to develop and defects at this early foundational stage have serious implications for overall neuronal function. Neuritogenesis is tightly regulated by various signaling mechanisms that control the timing and placement of neurite initiation, as well as the various processes necessary for neurite elongation to occur. Kinases are integral components of these regulatory pathways that control the activation and inactivation of their targets. This review provides a comprehensive summary of the kinases that are notably involved in regulating neurite formation, which is a complex process that involves cytoskeletal rearrangements, addition of plasma membrane to increase neuronal surface area, coupling of cytoskeleton/plasma membrane, metabolic regulation, and regulation of neuronal differentiation. Since kinases are key regulators of these functions during neuromorphogenesis, they have high potential for use as therapeutic targets for axon regeneration after injury or disease where neurite formation is disrupted.","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"12 1","pages":"1511-1530"},"PeriodicalIF":8.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75498044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disconnect between the developing eye and craniofacial prominences in the avian embryo 在禽类胚胎中，发育中的眼睛和颅面突起之间的脱节

IF 2.6

Mechanisms of Development Pub Date : 2020-03-01 DOI: 10.1016/j.mod.2020.103596

Jamil Jomaa , Jessica Martínez-Vargas , Shadya Essaili , Nida Haider , John Abramyan

{"title":"Disconnect between the developing eye and craniofacial prominences in the avian embryo","authors":"Jamil Jomaa , Jessica Martínez-Vargas , Shadya Essaili , Nida Haider , John Abramyan","doi":"10.1016/j.mod.2020.103596","DOIUrl":"10.1016/j.mod.2020.103596","url":null,"abstract":"<div>In the amniote embryo, the upper jaw and nasal cavities form through coordinated outgrowth and fusion of craniofacial prominences. Adjacent to the embryonic prominences are the developing eyes, which abut the maxillary and lateral nasal prominences. The embryos of extant sauropsids (birds and nonavian reptiles) develop particularly large eyes in comparison to mammals, leading researchers to propose that the developing eye may facilitate outgrowth of prominences towards the midline in order to aid prominence fusion. To test this hypothesis, we performed unilateral and bilateral ablation of the developing eyes in chicken embryos, with the aim of evaluating subsequent prominence formation and fusion. Our analyses revealed minor interaction between the developing craniofacial prominences and the eyes, inconsequential to the fusion of the upper beak. At later developmental stages, the skull exhibited only localized effects from missing eyes, while geometric morphometrics revealed minimal effect on overall shape of the upper jaw when it develops without eyes. Our results indicate that the substantial size of the developing eyes in the chicken embryo exert little influence over the fusion of the craniofacial prominences, despite their effect on the size and shape of maxillary prominences and components of the skull.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"161 ","pages":"Article 103596"},"PeriodicalIF":2.6,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2020.103596","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37631525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis of axolotl oropharyngeal explants during taste bud differentiation stages 美西螈味蕾分化阶段口咽外植体转录组分析

IF 2.6

Mechanisms of Development Pub Date : 2020-03-01 DOI: 10.1016/j.mod.2020.103597

Priya Kohli , Lauren Marazzi , Deborah Eastman

{"title":"Transcriptome analysis of axolotl oropharyngeal explants during taste bud differentiation stages","authors":"Priya Kohli , Lauren Marazzi , Deborah Eastman","doi":"10.1016/j.mod.2020.103597","DOIUrl":"10.1016/j.mod.2020.103597","url":null,"abstract":"<div>The Mexican salamander, Ambystoma mexicanum (Axolotl), is an excellent vertebrate model system to understand development and regeneration. Studies in axolotl embryos have provided important insights into taste bud development. Taste bud specification and determination occur in the oropharyngeal endoderm of axolotl embryos during gastrulation and neurulation, respectively, whereas taste bud innervation and taste cell differentiation occur later in development. Axolotl embryos are amenable to microsurgery, and tissue explants develop readily in vitro. We performed RNA-seq analysis to investigate the differential expression of genes in oropharyngeal explants at several stages of taste cell differentiation. Since the axolotl genome has only recently been sequenced, we used a Trinity pipeline to perform de novo assembly of sequencing reads. Linear models for RNA-seq data were used to identify differentially expressed genes. We found 1234 unique genes differentially expressed during taste cell differentiation stages. We validated four of these genes using RTqPCR and performed GO functional analysis. The differential expression of these genes suggests that they may play a role in taste cell differentiation in axolotls.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"161 ","pages":"Article 103597"},"PeriodicalIF":2.6,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2020.103597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37631523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development 斑马鱼咽袋的形态形成和角鳃软骨的发育需要Pax1a和Pax1b

IF 2.6

Mechanisms of Development Pub Date : 2020-03-01 DOI: 10.1016/j.mod.2020.103598

Yu-Hsiu Liu , Tz-Chi Lin , Sheng-Ping L. Hwang

{"title":"Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development","authors":"Yu-Hsiu Liu , Tz-Chi Lin , Sheng-Ping L. Hwang","doi":"10.1016/j.mod.2020.103598","DOIUrl":"10.1016/j.mod.2020.103598","url":null,"abstract":"<div>Pharyngeal arches are derived from all three germ layers and molecular interactions among the tissue types are required for proper development of subsequent pharyngeal cartilages; however, the mechanisms underlying this process are not fully described. Here we report that in zebrafish, Pax1a and Pax1b have overlapping and essential functions in pharyngeal pouch morphogenesis and subsequent ceratobranchial cartilage development. Both pax1a and pax1b are co-expressed in pharyngeal pouches, and time-lapse imaging of a novel Tg(pax1b:eGFP) enhancer trap line further revealed the sequential segmental development of pharyngeal pouches. Zebrafish pax1a−/−; pax1b−/− double mutant embryos generated by CRISPR-Cas9 mutagenesis exhibit unsegmented pharyngeal pouches 2–5 with small outpocketings. Endodermal expression of fgf3, tbx1 and edn1 is also absent in pharyngeal pouches 2–5 at 36 h post fertilization (hpf). Loss of ceratobranchial cartilage 1–4 and reduced or absent expression of dlx2a and hand2 in the pharyngeal arches 3–6 are observed in CRISPR mutant and morphant embryos that are deficient in both zebrafish pax1a and pax1b at 96 or 36 hpf. These results suggest that zebrafish Pax1a and Pax1b both regulate pharyngeal pouch morphogenesis by modulating expression of fgf3 and tbx1. Furthermore, our data support a model wherein endodermal Pax1a and Pax1b act through Fgf3 and Tbx-Edn1 signaling to non-autonomously regulate the development of ceratobranchial cartilage via expression of dlx2a and hand2.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"161 ","pages":"Article 103598"},"PeriodicalIF":2.6,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2020.103598","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37647742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Claudin-7b and Claudin-h are required for controlling cilia morphogenesis in the zebrafish kidney Claudin-7b和Claudin-h是控制斑马鱼肾脏纤毛形态发生所必需的

IF 2.6

Mechanisms of Development Pub Date : 2020-03-01 DOI: 10.1016/j.mod.2019.103595

Miaomiao Jin , Donglian Wang , Wenyan Xu , Hong Wang , Ying Cao

{"title":"Claudin-7b and Claudin-h are required for controlling cilia morphogenesis in the zebrafish kidney","authors":"Miaomiao Jin , Donglian Wang , Wenyan Xu , Hong Wang , Ying Cao","doi":"10.1016/j.mod.2019.103595","DOIUrl":"10.1016/j.mod.2019.103595","url":null,"abstract":"<div>Claudins are a family of proteins which are the most important components of the tight junctions. The location of Claudins on the renal tubule epithelial determines its paracellular transport characteristics, but whether Claudins have other functions in kidneys remains still unclear. Here, we showed that the transcripts encoding two Claudin family proteins, claudin-7b (cldn-7b) and claudin-h (cldn-h), were expressed in the transporting cells in the zebrafish pronephros. By knocking down of cldn-7b and cldn-h in zebrafish, we showed that these claudins morphants exhibited cystic kidneys accompanied with body curvature. Further analysis showed that down regulation of cldn-7b or cldn-h led to multiple defects in apico-basolateral polarity, cilia morphology and ciliary function in kidney. Moreover, the ciliary defect was confirmed by depletion of Cldn-7b or Cldn-h using CRISPR/Cas9 system. We also showed that both cldn-7b and cldn-h were genetically interacted with a well-known ciliary gene, arl13b. Deletion of arl13b led to curly cilia in the pronephros that phenocopied with cldn-7b and cldn-h morphants. Taken together, our data suggested that the tight junction protein, Cldn-7b and Cldn-h, regulate kidney development and function by affecting cilia morphology.</div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"161 ","pages":"Article 103595"},"PeriodicalIF":2.6,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2019.103595","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37500199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Editorial “MOD” 社论“MOD”

IF 2.6

Mechanisms of Development Pub Date : 2020-03-01 DOI: 10.1016/j.mod.2019.103576

Roberto Mayor

引用次数: 0