Mitochondrial DnaPub Date : 2015-06-01Epub Date: 2014-01-17DOI: 10.3109/19401736.2013.873932
Jie Fan, Chun-Hong Li, Wei Shi
{"title":"The complete mitochondrial genome of Java warty pig (Sus verrucosus).","authors":"Jie Fan, Chun-Hong Li, Wei Shi","doi":"10.3109/19401736.2013.873932","DOIUrl":"https://doi.org/10.3109/19401736.2013.873932","url":null,"abstract":"<p><p>In the present study, the complete mitochondrial genome sequence of the Java warty pig was reported for the first time. The total length of the mitogenome was 16,479 bp. It contained the typical structure, including 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and 1 non-coding control region (D-loop region) as that of most other pigs. The overall composition of the mitogenome was estimated to be 34.9% for A, 26.1% for T, 26.0% for C and 13.0% for G showing an A-T (61.0%)-rich feature. The mitochondrial genome analyzed here will provide new genetic resource to uncover pigs' evolution. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"481-2"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.873932","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32041741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete mitochondrial genome of the serotine bat (Eptesicus serotinus) in Korea.","authors":"Taek Woo Nam, Kwang Bae Yoon, Jae Youl Cho, Yung Chul Park","doi":"10.3109/19401736.2013.819503","DOIUrl":"https://doi.org/10.3109/19401736.2013.819503","url":null,"abstract":"<p><p>Serotine bats of Eptesicus serotinus are uncommon species in South Korea due to habit loss and destruction. We describe the characteristics of complete mitochondrial genome and gene organization of E. serotinus in this article. The complete mitogenome of E. serotinus is 16,665 bp long with a total base composition of 32.8% A, 29.8% T, 23.5% C and 13.8% G. The mitogenome consists of 13 protein-coding genes (11,410 bp), 2 rRNA (12S and 16S RNA) genes (2524 bp), 22 tRNA genes (1504 bp) and 1 control region (1225 bp). The results will contribute to understand the genetic structure of Korean E. serotinus. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"459-60"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.819503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31262063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-06-01Epub Date: 2013-09-05DOI: 10.3109/19401736.2013.823166
X H Bai, X W Guo, X J Zhang, W Song, Y H Li, W Luo, X J Cao, W M Wang
{"title":"Species identification and evolutionary inference of the genera Megalobrama and Parabramis (Cyprinidae: Cultrinae) in China.","authors":"X H Bai, X W Guo, X J Zhang, W Song, Y H Li, W Luo, X J Cao, W M Wang","doi":"10.3109/19401736.2013.823166","DOIUrl":"https://doi.org/10.3109/19401736.2013.823166","url":null,"abstract":"<p><p>The species boundaries and evolutionary relationships of two closely related genera, Megalobrama and Parabramis, were inferred from the partial mitochondrial cytochrome oxidase subunit I (COI) gene, NADH dehydrogenase subunit 2 (ND2) gene and their concatenated segment. Phylogenetic reconstructions showed that among the three breams, Megalobrama amblycephala and Megalobrama skolkovii are more closely related to each other than either is to Megalobrama terminalis. The taxonomy of M. pellegrini should be reconsidered. The divergence time estimation based on the assumption of a global molecular clock indicated that speciation and dispersal of the two genera might have occurred at approximately Pliocene to Late Pleistocene, due to major paleo-environmental events associated with monsoon evolution and the formation of the Three Gorges of the Yangtze River. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"357-66"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.823166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31706710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete mitogenome of Stripe-Backed Shrew, Sorex cylindricauda (Soricidae).","authors":"Shunde Chen, Feiyun Tu, Xiuyue Zhang, Wei Li, Guiying Chen, Hao Zong, Qiong Wang","doi":"10.3109/19401736.2013.855756","DOIUrl":"https://doi.org/10.3109/19401736.2013.855756","url":null,"abstract":"<p><p>The Stripe-Backed Shrew, Sorex cylindricauda belongs to the family Soricidae, and distributes in northwestern Yunnan, central Sichuan, southern Gansu and Shaanxi. In this study, the complete mitochondrial genome sequence of S. cylindricauda was determined. The mitogenome is 17,191 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 control region, with a base composition of 33.2% A, 30.2% T, 23.8% C and 12.8% G. The study contributes to illuminating taxonomic status of Stripe-Backed Shrew Sorex cylindricauda. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"477-8"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.855756","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32017419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-06-01Epub Date: 2013-10-14DOI: 10.3109/19401736.2013.840588
F Keith Barker, Sara Oyler-McCance, Diana F Tomback
{"title":"Blood from a turnip: tissue origin of low-coverage shotgun sequencing libraries affects recovery of mitogenome sequences.","authors":"F Keith Barker, Sara Oyler-McCance, Diana F Tomback","doi":"10.3109/19401736.2013.840588","DOIUrl":"https://doi.org/10.3109/19401736.2013.840588","url":null,"abstract":"<p><p>Next generation sequencing methods allow rapid, economical accumulation of data that have many applications, even at relatively low levels of genome coverage. However, the utility of shotgun sequencing data sets for specific goals may vary depending on the biological nature of the samples sequenced. We show that the ability to assemble mitogenomes from three avian samples of two different tissue types varies widely. In particular, data with coverage typical of microsatellite development efforts (∼1×) from DNA extracted from avian blood failed to cover even 50% of the mitogenome, relative to at least 500-fold coverage from muscle-derived data. Researchers should consider possible applications of their data and select the tissue source for their work accordingly. Practitioners analyzing low-coverage shotgun sequencing data (including for microsatellite locus development) should consider the potential benefits of mitogenome assembly, including internal barcode verification of species identity, mitochondrial primer development, and phylogenetics. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"384-8"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.840588","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31800231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete mitochondrial genome of Takydromus sexlineatus (Squamata, Lacertidae).","authors":"Ping-Sheng Qin, De-Long Zeng, Li-Xia Hou, Xiao-Wen Yang, Xin-Min Qin","doi":"10.3109/19401736.2013.830299","DOIUrl":"https://doi.org/10.3109/19401736.2013.830299","url":null,"abstract":"<p><p>The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"465-6"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.830299","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31742601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-06-01Epub Date: 2014-04-14DOI: 10.3109/19401736.2014.908355
Aiguo Zhou, Xiaolei Zhuo, Qing Zou, Jintao Chen, Jixing Zou
{"title":"Population genetic diversity of the northern snakehead (Channa argus) in China based on the mitochondrial DNA control region and adjacent regions sequences.","authors":"Aiguo Zhou, Xiaolei Zhuo, Qing Zou, Jintao Chen, Jixing Zou","doi":"10.3109/19401736.2014.908355","DOIUrl":"https://doi.org/10.3109/19401736.2014.908355","url":null,"abstract":"<p><p>Genetic variation and population structure of northern snakehead (Channa argus) from eight locations in China were investigated using mitochondrial DNA control region and adjacent regions sequences. Sequence analysis showed that there were 105 haplotypes in 260 individuals, 48 unique haplotypes and 57 shared haplotypes, but no common haplotype shared by all populations. As a whole, the haplotype diversity was high (h=0.989), while the nucleotide diversity was low (π=0.00482). AMOVA analysis detected significant genetic differentiation among all eight populations (FST=0.328, p<0.01) and 66.17% of the total variance was resulted from intra-population differentiation. UPGMA analysis indicated that the eight populations could be divided into four major clusters, which was consistent with that the eight sampled locations were belonged to four isolated river systems. The neutrality and mismatch distribution tests suggested that the eight populations of C. argus in the sampling locations underwent recent population expansion. Among the eight populations, the Erhai Lake population may represent a unique genetic resource and therefore needs to be conserved.</p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"341-9"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2014.908355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32256013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-04-01Epub Date: 2013-08-28DOI: 10.3109/19401736.2013.825773
Cristina Gutiérrez Povedano, Josefa Salgado, Carmen Gil, Maitane Robles, Ana Patiño-García, Jesús García-Foncillas
{"title":"Analysis of BRCA1 and mtDNA haplotypes and mtDNA polymorphism in familial breast cancer.","authors":"Cristina Gutiérrez Povedano, Josefa Salgado, Carmen Gil, Maitane Robles, Ana Patiño-García, Jesús García-Foncillas","doi":"10.3109/19401736.2013.825773","DOIUrl":"https://doi.org/10.3109/19401736.2013.825773","url":null,"abstract":"<p><p>Mitochondrial DNA (mtDNA) defects have been postulated to play an important role in the modulation and/or progression of cancer. In the past decade, a wide spectrum of mtDNA variations have been suggested as potentially sensitive and specific biomarkers for several human cancer types. In this context, single nucleotide polymorphisms (SNPs) described as protective or risk variants have been published, in particular in breast cancer, though not without controversy. Moreover, many mtDNA haplogroups have been associated with different phenotypes and diseases. We genotyped 18 SNPs, 15 of them defining European mtDNA haplogroups, including SNPs described as protective or risk variants, 7 SNPs that determine BRCA1 haplotypes and a BRCA1 intron 7 polymorphism. We included in this study 90 Caucasian unrelated women with breast cancer with familial criteria and 96 controls. Our aim was to clarify the importance of any of these SNPs, mitochondrial haplogroups and BRCA1 haplotypes in the modulation of breast cancer. We detected no significant differences in the distribution of BRCA1 haplotypes between patients and controls. Haplogroup U and the 12308G variant of mtDNA were overrepresented within the control group (p = 0.005 and p = 0.036, respectively) compared to breast cancer. Finally, we identified a significant association between the BRCA1 intron 7 polymorphism and BRCA1 haplotypes. Specifically, (TTC)6/6 and (TTC)6/7 genotypes with the seven polymorphic site cassette of \"H2-like\" haplotypes, and the (TTC)7/7 genotype associated with the \"H1-like\" haplotypes (p < 0.001).</p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 2","pages":"227-31"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.825773","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31689702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-04-01Epub Date: 2013-09-11DOI: 10.3109/19401736.2013.823176
Marianna Soroka, Artur Burzyński
{"title":"Complete female mitochondrial genome of Anodonta anatina (Mollusca: Unionidae): confirmation of a novel protein-coding gene (F ORF).","authors":"Marianna Soroka, Artur Burzyński","doi":"10.3109/19401736.2013.823176","DOIUrl":"https://doi.org/10.3109/19401736.2013.823176","url":null,"abstract":"<p><p>Freshwater mussels are among animals having two different, gender-specific mitochondrial genomes. We sequenced complete female mitochondrial genomes from five individuals of Anodonta anatina, a bivalve species common in palearctic ecozone. The length of the genome was variable: 15,637-15,653 bp. This variation was almost entirely confined to the non-coding parts, which constituted approximately 5% of the genome. Nucleotide diversity was moderate, at 0.3%. Nucleotide composition was typically biased towards AT (66.0%). All genes normally seen in animal mtDNA were identified, as well as the ORF characteristic for unionid mitochondrial genomes, bringing the total number of genes present to 38. If this additional ORF does encode a protein, it must evolve under a very relaxed selection since all substitutions within this gene were non-synonymous. The gene order and structure of the genome were identical to those of all female mitochondrial genomes described in unionid bivalves except the Gonideini.</p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 2","pages":"267-9"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.823176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31719616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial DnaPub Date : 2015-04-01Epub Date: 2013-09-11DOI: 10.3109/19401736.2013.823184
Anhui Chen, Rong Xia, Guangchun Lei, Cuizhang Fu
{"title":"Complete mitochondrial genome of Pseudorasbora elongata (Cypriniformes: Cyprinidae).","authors":"Anhui Chen, Rong Xia, Guangchun Lei, Cuizhang Fu","doi":"10.3109/19401736.2013.823184","DOIUrl":"https://doi.org/10.3109/19401736.2013.823184","url":null,"abstract":"<p><p>Phylogenetic placement of Pseudorasbora elongate remains unresolved. We determined the first complete mitochondrial genome of P. elongate that its mitogenome data should contribute to clarify the systematics of Pseudorasbora fishes. The mitogenome was 16,607 bp in length, including 13 typical protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region. The overall base composition of the heavy strain was 31.1% for A, 24.8% for C, 28.2% for T and 15.9% for G, with the A + T bias of 59.3%.</p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 2","pages":"250-1"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.823184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31720047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}