{"title":"六线草的线粒体全基因组。","authors":"Ping-Sheng Qin, De-Long Zeng, Li-Xia Hou, Xiao-Wen Yang, Xin-Min Qin","doi":"10.3109/19401736.2013.830299","DOIUrl":null,"url":null,"abstract":"<p><p>The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region. </p>","PeriodicalId":49805,"journal":{"name":"Mitochondrial Dna","volume":"26 3","pages":"465-6"},"PeriodicalIF":0.0000,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/19401736.2013.830299","citationCount":"5","resultStr":"{\"title\":\"Complete mitochondrial genome of Takydromus sexlineatus (Squamata, Lacertidae).\",\"authors\":\"Ping-Sheng Qin, De-Long Zeng, Li-Xia Hou, Xiao-Wen Yang, Xin-Min Qin\",\"doi\":\"10.3109/19401736.2013.830299\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region. </p>\",\"PeriodicalId\":49805,\"journal\":{\"name\":\"Mitochondrial Dna\",\"volume\":\"26 3\",\"pages\":\"465-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/19401736.2013.830299\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mitochondrial Dna\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/19401736.2013.830299\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2013/9/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mitochondrial Dna","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/19401736.2013.830299","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2013/9/19 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
采用长链PCR和保守引物步进法测定了雌雄鲤线粒体基因组全序列。基因组全长18943 bp，包含13个蛋白编码基因、2个rRNA基因、22个tRNA基因和1个控制区(CR)。其基因组成和序列与大多数鳞片类爬行动物相似。除COI以GTG为起始密码子外，其他蛋白编码基因均以ATG为起始密码子。七个基因(ATP8)。ND4L。ND5。Cytb。ND1。COI和ND6)以TAA、TAG、AGGA和AGA终止密码子结束，其余6个基因为不完全终止密码子T/TA。基因组总体碱基组成由大到小依次为A 31.48%、C 24.67%、T 30.79%和G 13.05%， A + T偏倚为62.27%。CR位于tRNA-Pro和tRNA-Phe基因之间，长度为3562 bp，在控制区发现了一些串联重复序列、保守元件(CSB1-3)和终止相关序列(TAS1-3)。
Complete mitochondrial genome of Takydromus sexlineatus (Squamata, Lacertidae).
The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region.
期刊介绍：
Previously published under the title DNA Sequence (Vols 1-19.3), Mitochondrial DNA accepts original high-quality reports based on mapping, sequencing and analysis of mitochondrial DNA and RNA. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, medical genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The editorial board will also consider manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences.
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