Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science最新文献_第2页

Broiler behavior: Influence of thermal stress, age, and period of the day 肉鸡行为:热应激、日龄和日龄的影响

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-06-05 DOI: 10.37496/rbz5220200239

S. Sgavioli, E. Santos, C. Domingues, D. Castiblanco, P. H. Rodrigues, C. Zeferino, A. R. Almeida, I. C. Boleli

{"title":"Broiler behavior: Influence of thermal stress, age, and period of the day","authors":"S. Sgavioli, E. Santos, C. Domingues, D. Castiblanco, P. H. Rodrigues, C. Zeferino, A. R. Almeida, I. C. Boleli","doi":"10.37496/rbz5220200239","DOIUrl":"https://doi.org/10.37496/rbz5220200239","url":null,"abstract":"- This study evaluated the behavior of broiler chickens subjected to thermal stress during different periods of the day and age of birds. A total of 180 one-day-old male broiler chicks were allocated to three chambers. Each chamber contained five boxes, with 12 chicks/box. Birds were assigned to a completely randomized design. The birds were filmed, and the images recorded every minute during the two periods: morning (from 08:00 to 09:00 h) and afternoon (from 17:00 to 18:00 h), at 7, 14, and 35 d of age. The frequency of the behavioral category (water intake, feed intake, resting, exploration, and comfort) was determined. Data were subjected to variance analysis for mixed models with rearing temperatures (cold, usual, and hot) and age of birds (7, 14, and 35 d) as subdivided plot, and periods of the day (morning and afternoon) as sub-subdivided plot. Birds at cold and usual temperatures showed higher feed intake than those at hot temperature in the morning. Birds exposed to cold and usual temperatures explored the environment less frequently at 35 d when compared with 14 d. However, chickens at hot temperature showed decreased exploration according to the increase of age. Birds spent more time feeding during the morning, regardless of age and rearing temperature. Also, during this period of the day, chickens spent most of the time exploring the environment. The frequency of feed intake and exploration decreases with the increase of age. The similarity of behavior between chickens reared at cold and usual temperatures may be due to a change in the thermal comfort zone of the birds. It suggests that the real thermal comfort temperature is between the two temperature ranges studied (cold and usual).","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"101 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in the typology of dairy systems producing artisanal cheese and those producing only raw milk in Paraná State, Brazil 巴西帕拉纳<e:1>州生产手工奶酪和仅生产原料牛奶的乳制品系统类型的差异

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-05-29 DOI: 10.37496/rbz5220220154

F. I. Bánkuti, Gabriella Oliveira, J. C. Damasceno, P. Lima, M. A. Zambom, M. D. M. Bouroullec

{"title":"Differences in the typology of dairy systems producing artisanal cheese and those producing only raw milk in Paraná State, Brazil","authors":"F. I. Bánkuti, Gabriella Oliveira, J. C. Damasceno, P. Lima, M. A. Zambom, M. D. M. Bouroullec","doi":"10.37496/rbz5220220154","DOIUrl":"https://doi.org/10.37496/rbz5220220154","url":null,"abstract":"- We sought to develop a typology describing structural, production, and socioeconomic characteristics of dairy systems that produce artisanal cheese and compare it with that of systems that produce only raw milk. Data on 204 raw milk producers and 58 artisanal cheese producers in Paraná State, Brazil, were collected through on-site surveys and subjected to descriptive analysis, factor analysis, and means tests. Descriptive analyses were applied to characterize the sample and artisanal cheese production processes. Factor analysis identified the following three typological components: system production capacity, herd breed and milking characteristics, and farmer social characteristics. Farmers were divided into two groups, as follows: non-cheese producers (NCP) and artisanal cheese producers (ACP). Groups of farmers were compared in terms of typological components. It was found that ACP have smaller structure and production scale and focus less on herd breed and practices for improving milk quality than NCP. These results suggest that artisanal cheese production is a strategy to add value to milk that does not meet institutional or market requirements for transactions with the dairy industry, providing a foothold for producers to remain in the dairy business. Groups of farmers (ACP and NCP) do not differ in social indicators of typology.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of dietary protein:lipid ratio on growth and body composition in bullfrog (Lithobates catesbeianus) 饲粮蛋白脂比对牛蛙生长和体成分的影响

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-05-29 DOI: 10.37496/rbz5220220104

J. Fonseca-Madrigal, Themis Sofía Andrade-López, C. Martínez-Palacios, M. Chávez-Sánchez, M. A. Olvera‐Novoa, P. Navarrete-Ramírez, L. Raggi, C. C. Martínez-Chávez, Sibila Concha-Santos, M. G. Ríos-Durán

{"title":"Effect of dietary protein:lipid ratio on growth and body composition in bullfrog (Lithobates catesbeianus)","authors":"J. Fonseca-Madrigal, Themis Sofía Andrade-López, C. Martínez-Palacios, M. Chávez-Sánchez, M. A. Olvera‐Novoa, P. Navarrete-Ramírez, L. Raggi, C. C. Martínez-Chávez, Sibila Concha-Santos, M. G. Ríos-Durán","doi":"10.37496/rbz5220220104","DOIUrl":"https://doi.org/10.37496/rbz5220220104","url":null,"abstract":"- A feeding trial was performed to assess dietary protein:lipid ratios for the grow-out phase of the bullfrog ( Lithobates catesbeianus ). Nine balanced isoenergetic diets were formulated, combining three different protein levels (300, 400, and 500 g kg −1 ) with three different lipid levels (50, 100, and 200 g kg −1 ), designated as P300/50L, P300/100L, P300/200L, P400/50L, P400/100L, P400/200L, P500/50L, P500/100L, and P500/200L. Additionally, a commercial fish feed, commonly used in Mexico to feed bullfrogs, was also tested during the experiment. Growth performance, animal performance parameters, carcass composition, and fatty acid profiles in muscle and liver were evaluated. The feeding trial results showed that all the experimental diets enhanced growth, feed conversion ratio, and frog-leg weight compared with the commercial diet. Bullfrogs had higher growth with 400 and 500 g kg −1 of dietary protein regardless of dietary lipid content. It was also notable that with the P500/200L diet, frogs doubled the weight of those fed the commercial diet. DHA, EPA, and total omega-3 fatty acids were double in muscle and two to eight times higher in the liver compared with the commercial diet. In all cases, the final proximal composition of carcass reflected the diet composition. It is suggested that a diet containing 400 g kg −1 of protein and 50 g kg −1 lipids (protein/lipid ratio: 7.4; gross energy: 18.2 MJ kg −1 ) is adequate for bullfrog performance during the grow-out phase to achieve market size in a shorter period, thus, reducing farming risks and production costs","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of milking hygiene, herd size, water hardness and temperature-humidity index on milk quality of dairy farms 挤奶卫生、畜群规模、水硬度和温湿指数对奶牛场牛奶品质的影响

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-05-12 DOI: 10.37496/rbz5220210189

M. López-Carlos, P. Hernández-Briano, J. I. Aguilera-Soto, O. Carrillo-Muro, C. A. Medina-Flores, F. Méndez-Llorente, C. F. Aréchiga-Flores

{"title":"Effect of milking hygiene, herd size, water hardness and temperature-humidity index on milk quality of dairy farms","authors":"M. López-Carlos, P. Hernández-Briano, J. I. Aguilera-Soto, O. Carrillo-Muro, C. A. Medina-Flores, F. Méndez-Llorente, C. F. Aréchiga-Flores","doi":"10.37496/rbz5220210189","DOIUrl":"https://doi.org/10.37496/rbz5220210189","url":null,"abstract":"- The objective of this study was to evaluate the effect of milking hygiene practices, herd size, water hardness, and temperature-humidity index (THI) on the physicochemical and microbiological characteristics of raw milk, and standard plate count (SPC) in milking machines of dairy farms in the central region of Mexico. Data were collected from fifty-three dairy farms during one year. The evaluated effects included milking hygiene conditions (good, medium, poor), herd size (1-50, 51-100, 101-150, ≥151 heads), water hardness (soft or moderately hard), and THI (comfortable or stressful). The increase in milking hygiene produced greater milk yield (MY) and energy corrected milk (ECM) but lower protein content, and decreased the individual bacterial count (IBC) and somatic cell count (SCC). The MY, ECM, protein content, IBC, and SCC were higher on bigger farms. The use of soft water reduced MY, IBC, and SCC, but improved fat, lactose, total solids (TS), and non-fat solids (NFS). Heat stress negatively affected fat, protein, TS, NFS, acidity, freezing point (FP), SCC, and methylene blue dye reduction test. Poor milking hygiene contributes to higher SPC in milking machine parts. Water hardness and THI did not affect SPC in all milking machine parts. Proper milking hygiene practices, larger herd size, softer water, lower THI, and adequate cleaning and disinfection of the milking machine parts benefits the physicochemical and microbiological quality of the milk.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deoxynivalenol concentrations in feed ingredients and swine diets measured by enzyme-linked immunosorbent assay and high-performance liquid chromatography 用酶联免疫吸附法和高效液相色谱法测定饲料原料和猪日粮中脱氧雪腐烯醇的浓度

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-05-12 DOI: 10.37496/rbz5220220155

Jong Young Ahn, D. Cheong, J. Jeong, B. Kim

{"title":"Deoxynivalenol concentrations in feed ingredients and swine diets measured by enzyme-linked immunosorbent assay and high-performance liquid chromatography","authors":"Jong Young Ahn, D. Cheong, J. Jeong, B. Kim","doi":"10.37496/rbz5220220155","DOIUrl":"https://doi.org/10.37496/rbz5220220155","url":null,"abstract":"- The objective was to compare deoxynivalenol (DON) concentrations in feed ingredients and commercial swine diets measured by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Seventy feed ingredient samples consisted of corn, corn dried distillers grains with solubles, corn gluten feed, corn gluten meal, palm kernel expellers, rice bran, soy hulls, soybean meal, and wheat. Commercial swine diet samples (n = 92) were collected from 23 swine farms of varying regions in Korea and different growth stages of pigs. The DON concentration of all samples was determined in duplicate. Statistical comparisons were performed to compare the analytical methods (ELISA vs. HPLC), diet phases, and regions. The DON concentrations in most ingredients and all diets determined by ELISA method were greater than those determined by HPLC. The DON concentrations determined by the ELISA method were less than 1 mg/kg in all ingredients except corn dried distillers grains with solubles and corn gluten feed, and those determined by the HPLC were less than 0.5 mg/kg in all ingredients. The DON concentrations in complete diets did not vary by region or growth stages of pigs. The DON concentrations in most feed ingredients and commercial swine diets determined by ELISA method are greater than those determined by HPLC, but does not vary by regions in Republic of Korea or pig growth stages.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Expression analysis of m6A-related genes in various tissues of Meishan pigs at different developmental stages 梅山猪不同发育阶段各组织中m6a相关基因的表达分析

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-04-17 DOI: 10.37496/rbz5220210149

Yanan Cao, Shuoshuo Zhang, Guangzheng Wang, Shuai Zhang, Wen-hua Bao, Shenglong Wu

引用次数: 0

Effects of amylin on egg quality traits of hens during the early laying period 胰淀素对蛋鸡产蛋前期蛋品质性状的影响

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-04-17 DOI: 10.37496/rbz5220210207

S. Guzel

{"title":"Effects of amylin on egg quality traits of hens during the early laying period","authors":"S. Guzel","doi":"10.37496/rbz5220210207","DOIUrl":"https://doi.org/10.37496/rbz5220210207","url":null,"abstract":"- This study was conducted to investigate the effects of amylin, a pancreatic hormone polypeptide, on egg quality traits in laying hens. A total of 40 birds (14 wk of age) were randomly divided into two treatments with two replicates, 10 birds in each replicate. Twenty birds were subcutaneously injected with amylin at 75 µg/kg every other day (treatment 1), and the remaining animals (20 hens) were given only water as the control group (treatment 2). Eggs collected from the two groups (140 eggs per group) were examined for several quality performance traits such as egg weight, specific gravity, shape index, eggshell strength, eggshell thickness, eggshell ash, eggshell weight, Haugh unit (HU), albumen index, yolk index, yolk color, and albumen height. The results indicated that amylin had a positive effect on some egg quality traits in laying hens. Specific gravity, eggshell thickness, eggshell weight, HU, albumen index, yolk index, and albumen height, which are all considered as important quality parameters from the consumer point of view, especially HU, were relatively improved in the amylin treatment group. These results showed that the injection of 75 µg/kg amylin has a significant effect on some egg quality traits. Considering that egg quality characteristics are crucial for the egg industry, this study can be a reference for the detailed investigation of the use of amylin in the different stages of egg production.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolizable energy equivalence of guanidinoacetic acid in corn soybean meal-based broiler diets 玉米豆粕型肉鸡饲粮中胍基乙酸代谢能当量的研究

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-04-17 DOI: 10.37496/rbz5220220071

Hallef Rieger Salgado, G. C. Rocha, T. Petrolli, Marlene Schmidt, J. A. Rivera, R. Nunes, S. Borges, A. A. Calderano

{"title":"Metabolizable energy equivalence of guanidinoacetic acid in corn soybean meal-based broiler diets","authors":"Hallef Rieger Salgado, G. C. Rocha, T. Petrolli, Marlene Schmidt, J. A. Rivera, R. Nunes, S. Borges, A. A. Calderano","doi":"10.37496/rbz5220220071","DOIUrl":"https://doi.org/10.37496/rbz5220220071","url":null,"abstract":"- In this study, we evaluated how guanidinoacetic acid (GAA) addition in diets with various metabolizable energy (ME) contents affects the performance of broiler chickens. We also estimated the equivalence of GAA in ME. We distributed 1,280 one-day-old broilers in a completely randomized design with eight treatments, eight replicates, and twenty birds per experimental unit. Treatments were based on ME levels (2,775-2,875-2,975 kcal/kg; 2,850-2,950-3,050 kcal/kg; 2,925-3,025-3,125 kcal/kg; or 3,000-3,100-3,200 kcal/kg, from 1 to 7, 8 to 21, and 22 to 42 days of age) and the inclusion of GAA (0 or 600 mg/kg). Supplementation of GAA increased weight gain in broilers at an energy level of 2,908 kcal/kg and improved feed conversion ratio (FCR) at energy levels of 2,908 and 2,983 kcal/kg. There was a linear reduction in feed intake and an improvement in FCR of broilers with increasing levels of energy in diets, with and without GAA addition. Solving the equivalence equation, by applying each of the weighted average energy levels studied. indicates the GAA equivalence of 133, 103, 74, and 44 kcal/kg of diet. In conclusion, GAA supplementation improves broilers’ efficiency of energy use; the average ME equivalence of 600 mg/kg of GAA is 88.5 kcal/kg","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time polymerase chain reaction for detection and enumeration of Staphylococcus aureus and Streptococccus agalactiae using different milk samplings 实时聚合酶链反应检测和枚举不同牛奶样品的金黄色葡萄球菌和无乳链球菌

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-04-07 DOI: 10.37496/rbz5220210086

A. G. Dibbern, J. L. Gonçalves, B. G. Alves, J. R. Barreiro, Kevin Anderson, Marcos Veiga dos Santos

{"title":"Real-time polymerase chain reaction for detection and enumeration of Staphylococcus aureus and Streptococccus agalactiae using different milk samplings","authors":"A. G. Dibbern, J. L. Gonçalves, B. G. Alves, J. R. Barreiro, Kevin Anderson, Marcos Veiga dos Santos","doi":"10.37496/rbz5220210086","DOIUrl":"https://doi.org/10.37496/rbz5220210086","url":null,"abstract":"- The objective of the present study was to evaluate the qPCR for detection and enumeration of Staphylococcus aureus and Streptococcus agalactiae using different milk samplings in comparison to the conventional microbiology. Four dairy herds with a history of subclinical mastitis caused by S . aureus and S . agalactiae were selected. Sampling approach included milk samples from bulk tank (BT), cow level (composite samples, CO), and mammary quarter level (MQ) from 785 lactating cows. Three consecutive monthly milk samplings were carried out, totaling 3347 MQ milk samples, 912 CO, and 12 from BT. All collected milk samples were subjected to conventional microbiology and qPCR for detection and enumeration of S . aureus and S . agalactiae. The qPCR showed 71.5% of diagnostic sensitivity for S . aureus isolated from MQ milk samples, 71.8% for CO, and 50% for BT milk samples compared with conventional microbiology methodology. Taken together, the diagnostic sensitivity for S . agalactiae isolated from MQ milk samples was 90.2, 87.7 for CO, and 90.9% for BT milk samples. In general, the qPCR methodology enabled the detection of S . aureus and S . agalactiae , regardless of the type of milk sampling. The direct use of milk samples to estimate the counting of S . aureus by qPCR demonstrated lower sensitivity than the counting of S . agalactiae , which can be explained by the","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key genes in bovine mammary epithelial cells challenged with Escherichia coli and Staphylococcus aureus by integrated bioinformatics analysis 应用综合生物信息学分析鉴定大肠杆菌和金黄色葡萄球菌侵染牛乳腺上皮细胞的关键基因

IF 1 4区农林科学

Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science Pub Date : 2023-04-07 DOI: 10.37496/rbz5220220064

Zeshi Chen, Zhengyu Bian, Xiuli Chen, Boling Li, Lianbin Li

{"title":"Identification of key genes in bovine mammary epithelial cells challenged with Escherichia coli and Staphylococcus aureus by integrated bioinformatics analysis","authors":"Zeshi Chen, Zhengyu Bian, Xiuli Chen, Boling Li, Lianbin Li","doi":"10.37496/rbz5220220064","DOIUrl":"https://doi.org/10.37496/rbz5220220064","url":null,"abstract":"- This study integrated four microarray datasets by Robust Rank Aggregation (RRA) method to identify the differentially expressed genes (DEG) in bovine mammary epithelial (BME) cells in response to Escherichia coli and Staphylococcus aureus infection. Furthermore, the GO function and KEGG pathway enrichment analysis of the integrated DEG were performed. Finally, the protein-protein interaction (PPI) network was constructed. A total of 72 integrated DEG were identified from the four datasets. The most significantly enriched terms within the integrated DEG were mainly involved in the immune response. The PPI network of DEG was constructed with 53 nodes. Seventeen genes, which constitute a significant module, were identified as hub genes. Among them, CD40, CXCL6 , and NFKBIZ were further screened as the key genes and have the potential to become biomarkers of E. coli and S. aureus mastitis, considering the specificity of biomarkers for diseases. The identified key genes and pathways in this study can assist in the search for biomarkers for mastitis diagnosis and disease resistance breeding.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0