Effect of dietary protein:lipid ratio on growth and body composition in bullfrog (Lithobates catesbeianus)

- A feeding trial was performed to assess dietary protein:lipid ratios for the grow-out phase of the bullfrog ( Lithobates catesbeianus ). Nine balanced isoenergetic diets were formulated, combining three different protein levels (300, 400, and 500 g kg −1 ) with three different lipid levels (50, 100, and 200 g kg −1 ), designated as P300/50L, P300/100L, P300/200L, P400/50L, P400/100L, P400/200L, P500/50L, P500/100L, and P500/200L. Additionally, a commercial fish feed, commonly used in Mexico to feed bullfrogs, was also tested during the experiment. Growth performance, animal performance parameters, carcass composition, and fatty acid profiles in muscle and liver were evaluated. The feeding trial results showed that all the experimental diets enhanced growth, feed conversion ratio, and frog-leg weight compared with the commercial diet. Bullfrogs had higher growth with 400 and 500 g kg −1 of dietary protein regardless of dietary lipid content. It was also notable that with the P500/200L diet, frogs doubled the weight of those fed the commercial diet. DHA, EPA, and total omega-3 fatty acids were double in muscle and two to eight times higher in the liver compared with the commercial diet. In all cases, the final proximal composition of carcass reflected the diet composition. It is suggested that a diet containing 400 g kg −1 of protein and 50 g kg −1 lipids (protein/lipid ratio: 7.4; gross energy: 18.2 MJ kg −1 ) is adequate for bullfrog performance during the grow-out phase to achieve market size in a shorter period, thus, reducing farming risks and production costs