Revista Romana De Medicina De Laborator最新文献_第9页

Essentials in the diagnosis of postoperative myocardial lesions similar to or unrelated to rejection in heart transplant 与心脏移植排斥反应相似或无关的术后心肌病变诊断要点

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0018

Costel Dumitru, A. Zazgyva, A. Habor, O. Cotoi, H. Suciu, C. Cotrutz, B. Grecu, I. Sin

{"title":"Essentials in the diagnosis of postoperative myocardial lesions similar to or unrelated to rejection in heart transplant","authors":"Costel Dumitru, A. Zazgyva, A. Habor, O. Cotoi, H. Suciu, C. Cotrutz, B. Grecu, I. Sin","doi":"10.2478/rrlm-2021-0018","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0018","url":null,"abstract":"Abstract Background, objectives: Histological diagnosis of control biopsies in patients with heart transplant represents a significant step of monitoring, with a great influence on adjusting immunosuppressive treatment. Histological lesions are usually related to ischemia and reperfusion, with varying degrees of intensity. This study aimed to highlight the most important aspects of the histological diagnosis and differential diagnosis of postoperative myocardial lesions associated or unrelated to rejection in heart transplant. Materials and Methods: This retrospective study involved 53 patients who received cardiac transplant between 2000 and 2017. Patients were monitored by lesion quantification of endomyocardial biopsies, with diagnoses established based on biopsy material in the early, medium and late post-transplant periods. Hematoxylin eosin, Masson’s trichrome, and Van Gieson stains were used; immunohistochemical determinations used CD4, CD20, CD45, CD68, HLA-DR, VEGF and CD31. Results: Ischemia and reperfusion lesions were diagnosed on all biopsies in the first 6 weeks post-transplant. Nine cases of the Quilty effect were identified, and in 12 cases, the biopsies were performed on the same spot as previous biopsies. A significant number of transplanted patients presented cytomegalovirus that was difficult to diagnose on endomyocardial biopsies. Conclusions: The detailed study of ischemia and reperfusion lesions, as well as of changes un-related to rejection becomes a major objective in the short, medium and late post-transplant period. Overdiagnosis of rejection induces changes of the immunosuppressive therapeutic protocol, with alarming repercussions on cytomegalovirus reactivation, and risks of potentiating inflammation, myocyte destruction and the recurrence of disorders related to both inducing and aggravating heart failure.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"9 1","pages":"307 - 318"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75480774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lupus anticoagulant in children – a confounding factor in diagnosis and targeted therapy 儿童狼疮抗凝剂-诊断和靶向治疗的混淆因素

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0021

C. Jinca, M. Serban, E. Ursu, N. Paşcalău, O. Belei, D. Săvescu, M. Lelik, A. Munteanu, A. Tiede, S. Arghirescu

{"title":"Lupus anticoagulant in children – a confounding factor in diagnosis and targeted therapy","authors":"C. Jinca, M. Serban, E. Ursu, N. Paşcalău, O. Belei, D. Săvescu, M. Lelik, A. Munteanu, A. Tiede, S. Arghirescu","doi":"10.2478/rrlm-2021-0021","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0021","url":null,"abstract":"Abstract Introduction: Lupus anticoagulant (LAC) belongs to a heterogeneous group of antibodies directed against negatively charged phospholipid-binding proteins, inhibiting phospholipid-dependent reactions. We assessed the frequency, etiological background, clinical and biological expression as well as the appropriate management and outcome of LAC in a pediatric population. Patients and methods: We included 19 pediatric patients diagnosed with a positive LAC test during a period of 1 year. LAC detection was carried out according to the ISTH/SCC guidelines 2012. Coagulometric assays were performed in order to check for coagulation factor activities. The presence of specific inhibitors has been tested by the Bethesda assay. Results: LAC was positive in 0.4% of the patients monitored for increased activated partial thromboplastin time (aPTT) during the study period of 1 year. In contrast to the asymptomatic clinical image presented by the majority of our patients, the biological picture was dominated by an elevated aPTT (79 %), the aPTT ratio correlating significantly with the strength of LAC. In 58 % of the patients LAC became negative within 12 weeks, in another 4 (21%) patients within 20 weeks, whereas in 4 (21%) patients LAC remained positive throughout the follow-up. Conclusion: Albeit usually innocent in asymptomatic patients, LAC needs to be seriously taken into consideration from the diagnostic point of view in order to avoid dangerous therapeutic attitudes..","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"35 1","pages":"287 - 298"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79783897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction factor between two calcitonin assays: DiaSorin LiaisonXL and Cobas E601 两种降钙素测定法之间的校正因子:DiaSorin LiaisonXL和Cobas E601

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0024

M. Băetu, A. Drăghici, A. Pădure, Ș. Bucur, A. Caragheorgheopol, C. Badiu

{"title":"Correction factor between two calcitonin assays: DiaSorin LiaisonXL and Cobas E601","authors":"M. Băetu, A. Drăghici, A. Pădure, Ș. Bucur, A. Caragheorgheopol, C. Badiu","doi":"10.2478/rrlm-2021-0024","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0024","url":null,"abstract":"Abstract Introduction: Calcitonin measurement is important for the diagnosis and monitoring of medullary thyroid carcinoma. Unfortunately, in clinical practice, different detection systems assays are used in the follow-up of the patients, which can be misleading. Objective: To identify the correction factor for calcitonin measurement on two different immunoanalysers: DiaSorin-LiaisonXL (immunochemiluminescence) and CobasE601 (electro-immunochemiluminescence). Methods: We selected 89 registered CT samples (28-from men; 61-from women), that were analysed on CobasE601 with reported values between 0.5 pg/ml and 2812 pg/ml (128.5±513.98). These CT samples were selected randomly to cover as wide a range of values as possible, and represented either basal CT (n=38) or selected from CT stimulation tests (n=51). Samples were evaluated subsequently on DiaSorin-LiaisonXL. All patients gave their informed consent. Results: Between the two assays a segmented linear correlation was noted. We identified the following general linear regression equation: 1.108x+19.337 (p<0.05). The bias increased at high calcitonin values. Therefore, for a better accuracy we analysed the regression equation segmentally. A statistic difference (p<0.05) was noted for CT values ranged between 350-2600 pg/ml (n=31) on DiaSorin-LiaisonXL, for which the linear regression equation for CobasE601 becomes 1.009x+169.796. Conclusions: Calcitonin correction factors are highly important in the dynamic follow up of a patient suffering from medullary thyroid carcinoma when different detection systems assays are used to determine calcitonin. We identified the correction factors for calcitonin determination between two different frequently used chemiluminescence immunoanalysers: DiaSorin-LiaisonXL and CobasE601. However, it is strongly advisable to use the same analyser in order to establish biochemical evolution of calcitonin.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"77 1","pages":"271 - 276"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85660927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas miR-155-5p和局部基质Gla蛋白在脑膜瘤中的表达

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0020

Simona R. Gheorghe, C. Marian, L. Tătăranu, A. Dricu, C. Vermeer, C. Silaghi, A. Crăciun

{"title":"The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas","authors":"Simona R. Gheorghe, C. Marian, L. Tătăranu, A. Dricu, C. Vermeer, C. Silaghi, A. Crăciun","doi":"10.2478/rrlm-2021-0020","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0020","url":null,"abstract":"Abstract Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence of calcification in meningiomas influences their growth rate. The messenger RNA of matrix Gla protein (MGP), an extra-hepatic protein with different conformations involved in the homeostasis of ectopic calcification has been found in meningiomas and was shown to be regulated in breast cancer by miR-155-5p, a specific micro RNA. Therefore, we investigated the expression of miR-155-5p and its relationship with local MGP conformations in different grade meningiomas. According to the WHO classification, our 41 samples of meningiomas were stratified in groups WHO I and WHO II. Using real time polymerase chain reaction, we observed a higher miR-155-5p expression in group WHO I versus group WHO II [with a fold change (FC) of 3.83, p=0.027)]. Moreover, the expression of miR-155-5p was higher in calcified tumors compared to non-calcified tumors in all samples (FC=3.01, p=0.047) and in group WHO I (FC=3.65, p=0.048). Utilizing immunohistochemistry, we determined the concurrent presence of all MGP conformations in calcified meningiomas. This study was the first to establish higher miR-155-5p expression in grade WHO I and calcified meningiomas, which could improve molecular classification and targeted therapy and also the presence of all MGP conformations in calcified meningiomas, confirming the existence of an anti-calcification mechanism in meningiomas..","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"21 1","pages":"299 - 306"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74814837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deity, love, punishment, rage, and mythonyms from head to toes. A brief history of some medical terms 神，爱，惩罚，愤怒，从头到脚都是神话。一些医学术语的简史

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0023

Adrian Naznean

引用次数: 0

Genetic Diagnostic Approaches in Familial Hypercholesterolemia Evaluation 家族性高胆固醇血症评估的遗传诊断方法

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0025

V. Moldovan, C. Bănescu, M. Dobreanu

{"title":"Genetic Diagnostic Approaches in Familial Hypercholesterolemia Evaluation","authors":"V. Moldovan, C. Bănescu, M. Dobreanu","doi":"10.2478/rrlm-2021-0025","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0025","url":null,"abstract":"Abstract Familial hypercholesterolemia (FH) manifested as atherosclerosis is a major cause of coronary heart disease. Different scoring systems based on clinical and paraclinical data are currently used, but the FH diagnosis should be made only in the presence of the causative genetic defect. In the present study, 12 symptomatic (previously diagnosed with atherosclerosis) and asymptomatic family members were investigated. Serum lipids were measured using commercial reagents. A genetic investigation was performed by Sanger sequencing using commercial reagents and custom primers, while copy number variations and a selected set of 40 point mutations were evaluated using in vitro diagnostic medical devices. For the investigated patients, serum lipids were within the reference range, due to the fact that the subjects were following lipid-lowering therapy, and smoking was the only identifiable additional risk factor. Four benign exon variants and three intron variants situated within the low-density lipoprotein cholesterol receptor gene were identified by Sanger sequencing. No copy number variations and none of the 40 investigated point mutations were determined. Although independently considered benign, the combined effect of the identified genetic conditions could be pathogenic under the influence of additional risk factors. Even in the presence of a diagnosis made using clinical scores, the molecular diagnosis is often challenging, attesting to the complexity of FH genetic etiology.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"181 1","pages":"319 - 325"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80199199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Innate Immune Response Against SARS-CoV-2 Infection 对SARS-CoV-2感染的先天免疫反应的见解

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0022

A. Huțanu, A. Georgescu, Ákos Vince Andrejkovits, W. Au, M. Dobreanu

{"title":"Insights into Innate Immune Response Against SARS-CoV-2 Infection","authors":"A. Huțanu, A. Georgescu, Ákos Vince Andrejkovits, W. Au, M. Dobreanu","doi":"10.2478/rrlm-2021-0022","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0022","url":null,"abstract":"Abstract The innate immune system is mandatory for the activation of antiviral host defense and eradication of the infection. In this regard, dendritic cells, natural killer cells, macrophages, neutrophils representing the cellular component, and cytokines, interferons, complement or Toll-Like Receptors, representing the mediators of unspecific response act together for both activation of the adaptive immune response and viral clearance. Of great importance is the proper functioning of the innate immune response from the very beginning. For instance, in the early stages of viral infection, the defective interferon response leads to uncontrolled viral replication and pathogen evasion, while hypersecretion during the later stages of infection generates hyperinflammation. This cascade activation of systemic inflammation culminates with cytokine storm syndrome and hypercoagulability state, due to a close interconnection between them. Thus an unbalanced reaction, either under- or over- stimulation of the innate immune system will lead to an uncoordinated response and unfavorable disease outcomes. Since both cellular and humoral factors are involved in the time-course of the innate immune response, in this review we aimed to address their gradual involvement in the antiviral response with emphasis on key steps in SARS-CoV-2 infection.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"22 1","pages":"255 - 269"},"PeriodicalIF":0.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72502890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The effects of microwave radiation generated by mobile phones on the tear film 手机产生的微波辐射对泪膜的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-07-01 DOI: 10.2478/rrlm-2021-0019

Aurora Gajta, A. Jurca, A. Stănilă, D. Stănilă

引用次数: 1

Frequency, Distribution and Genotyping of Malassezia Species in Patients with Psoriasis vulgaris 寻常型银屑病患者马拉色菌的频率、分布及基因分型分析

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-04-01 DOI: 10.2478/rrlm-2021-0011

E. Çelik, N. Duran, D. Balcı, A. Doğramacı, O. Pasa

{"title":"Frequency, Distribution and Genotyping of Malassezia Species in Patients with Psoriasis vulgaris","authors":"E. Çelik, N. Duran, D. Balcı, A. Doğramacı, O. Pasa","doi":"10.2478/rrlm-2021-0011","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0011","url":null,"abstract":"Abstract Background: Malassezia species are reported to play a role in the etiology of Psoriasis vulgaris. Objective: The aim of this study was to determine the presence, frequency, distribution, and genotyping of skin colonization of Malassezia species in Psoriasis vulgaris and to compare with healthy individuals and to investigate its relationship with the severity of the disease. Methods: Skin samples were taken from scalp, arm, body, and leg of 34 psoriasis patients (lesional/non-lesional skin) and 30 healthy volunteers. Overall, 392 skin scraping samples were taken for the isolation of Malassezia species, which were incubated on the modified-Dixon agar. Conventional culture methods were used for Malassezia species identification. In isolates, genotyping was carried out by PCR-RFLP method. Results: In the samples from psoriatic lesions, most frequently isolated Malassezia species were M.globosa and M.furfur. Similarly, the most frequently isolated species in healthy volunteers was M. globosa; followed by M.restricta and M.sympodialis. The M.furfur isolation rate in psoriatic scalp and leg lesions of the patients was significantly higher than in healthy volunteers. There was no relationship between the severity of the disease and the isolated species. Conclusion: It was found that there was a difference between patients with psoriasis and healthy controls regarding presence and frequency of Malassezia species. Therefore, our study results support the view that Malessezia species may be associated with the etiopathogenesis of psoriasis. In addition, we surmise that the treatment applications for the regulation of skin microbiota of psoriasis patients will contribute positively to the treatment of psoriasis.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"26 6 1","pages":"217 - 227"},"PeriodicalIF":0.5,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89837563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of ∆F508 cystic fibrosis carriers in a Romanian population group 罗马尼亚人群中∆F508囊性纤维化携带者的患病率

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-04-01 DOI: 10.2478/rrlm-2021-0009

Sergiu Oșan, Iona Hrapșa, C. Coroama, D. Miclea, C. Al-Khzouz, C. Lazar, M. Farcas

引用次数: 0