miR-155-5p和局部基质Gla蛋白在脑膜瘤中的表达

IF 0.5 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Simona R. Gheorghe, C. Marian, L. Tătăranu, A. Dricu, C. Vermeer, C. Silaghi, A. Crăciun
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引用次数: 0

摘要

世界卫生组织(WHO)根据脑膜瘤的形态学特征将其分为三个等级。与分级无关,脑膜瘤中钙化的存在影响其生长速度。基质Gla蛋白(MGP)的信使RNA是一种具有不同构象的肝外蛋白,参与异位钙化的稳态,已在脑膜瘤中发现,并被证明在乳腺癌中受miR-155-5p(一种特异性微RNA)的调节。因此,我们研究了不同级别脑膜瘤中miR-155-5p的表达及其与局部MGP构象的关系。根据WHO的分类,我们的41例脑膜瘤样本被分为WHO I组和WHO II组。通过实时聚合酶链反应,我们观察到WHO I组miR-155-5p的表达高于WHO II组[fold change (FC)为3.83,p=0.027]。此外,在所有样本中,miR-155-5p在钙化肿瘤中的表达高于非钙化肿瘤(FC=3.01, p=0.047),在WHO I组中(FC=3.65, p=0.048)。利用免疫组织化学,我们确定了钙化脑膜瘤中所有MGP构象的同时存在。本研究首次发现miR-155-5p在WHO I级及钙化脑膜瘤中表达较高,可提高分子分类和靶向治疗水平,且钙化脑膜瘤中存在所有MGP构象,证实脑膜瘤中存在抗钙化机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas
Abstract Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence of calcification in meningiomas influences their growth rate. The messenger RNA of matrix Gla protein (MGP), an extra-hepatic protein with different conformations involved in the homeostasis of ectopic calcification has been found in meningiomas and was shown to be regulated in breast cancer by miR-155-5p, a specific micro RNA. Therefore, we investigated the expression of miR-155-5p and its relationship with local MGP conformations in different grade meningiomas. According to the WHO classification, our 41 samples of meningiomas were stratified in groups WHO I and WHO II. Using real time polymerase chain reaction, we observed a higher miR-155-5p expression in group WHO I versus group WHO II [with a fold change (FC) of 3.83, p=0.027)]. Moreover, the expression of miR-155-5p was higher in calcified tumors compared to non-calcified tumors in all samples (FC=3.01, p=0.047) and in group WHO I (FC=3.65, p=0.048). Utilizing immunohistochemistry, we determined the concurrent presence of all MGP conformations in calcified meningiomas. This study was the first to establish higher miR-155-5p expression in grade WHO I and calcified meningiomas, which could improve molecular classification and targeted therapy and also the presence of all MGP conformations in calcified meningiomas, confirming the existence of an anti-calcification mechanism in meningiomas..
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来源期刊
Revista Romana De Medicina De Laborator
Revista Romana De Medicina De Laborator MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
0.31
自引率
20.00%
发文量
43
审稿时长
>12 weeks
期刊介绍: The aim of the journal is to publish new information that would lead to a better understanding of biological mechanisms of production of human diseases, their prevention and diagnosis as early as possible and to monitor therapy and the development of the health of patients
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