Biotechnology for Biofuels最新文献

{"title":"There is an “I” in team: individual improvements in supercharged cellulase cocktail facilitates cooperative cellulose degradation","authors":"Antonio DeChellis,&nbsp;Samantha Shimabukuro,&nbsp;Sumay Trivedi,&nbsp;Reena Lubowski,&nbsp;Bhargava Nemmaru,&nbsp;Shishir P. S. Chundawat","doi":"10.1186/s13068-026-02740-y","DOIUrl":"10.1186/s13068-026-02740-y","url":null,"abstract":"<div><p>Lignocellulosic biomass is an abundant renewable carbon source for biofuel production, but its conversion to fermentable sugars is hindered by poor cellulase activity on highly crystalline and insoluble cellulose. While pretreatment makes biomass more amenable to enzymatic degradation, several issues linger related to productive enzyme binding and efficient catalytic turnover. To address this bottleneck, we employed protein supercharging to rationally design a glycosyl hydrolase (GH) family-6 exocellulase (Cel6B) and its native family-2a carbohydrate binding module (CBM2a) from the thermophilic cellulolytic microbe <i>Thermobifida fusca</i>. A chimeric library of 32 supercharged constructs rationally designed across both GH/CBM domains was synthesized and expressed in <i>E. coli</i>. Screening of the entire library of supercharged enzymes on several cellulosic substrates identified one key construct, D5 CBM2a–WT Cel6B, containing a positively supercharged CBM2a that showed 2–threefold higher activity on all substrates tested at pH 5.5. Purified enzyme assays confirmed that exocellulases behave quite differently from their endocellulase counterparts when supercharged using similar protocols. Still, the purified D5 CBM2a–WT Cel6B mutant showed a 2.3-fold improvement in specific activity compared to native enzyme on crystalline cellulose. Analysis of melt curves depicts that, while all other constructs tested have one distinct melt peak near the expected CBM melting point, domain melting is decoupled for the D5 CBM2a mutant. This effect reveals an intrinsic melting temperature of the Cel6B CD nearly 18 °C higher than the coupled melting temperature of the full-length enzyme. This unexpected stabilization effect of supercharged CBM2a domain is likely the driving force for activity improvements seen for this exocellulase that is otherwise prone to stalling and denaturation on the cellulose surface during processive catalytic turnover cycles. When combining this supercharged exocellulase construct with its endocellulase counterpart, our results showed that supercharged enzymes, exhibiting the highest activity alone, produced the best synergistic partners. This study highlights another successful implementation of protein supercharging for cellulases and provides another key piece toward building an effective synergistic cellulase cocktail for lignocellulosic biomass deconstruction.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12874829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146044439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-tuning xylan side chains for enhanced production of high-yield sugars, fine-shaped nanocrystals, and stable Pickering emulsions in Arabidopsis thaliana","authors":"Sufang Li,&nbsp;Min Li,&nbsp;Hailang Wang,&nbsp;Tianqi Li,&nbsp;Zhen Hu,&nbsp;Yanting Wang,&nbsp;Peng Chen,&nbsp;Zhaosheng Kong,&nbsp;Liangcai Peng,&nbsp;Youmei Wang","doi":"10.1186/s13068-026-02739-5","DOIUrl":"10.1186/s13068-026-02739-5","url":null,"abstract":"<div><p>Arabinoxylans are complex polysaccharides found in the secondary cell walls of plants, and their substitution patterns of arabinose (Ara) and glucuronic acid (GlcA) significantly affect their properties for structural and bio-functional applications. In this study, we engineered xylan side chains by overexpressing the arabinosyltransferase <i>AtXAT2</i> in both wild-type <i>Arabidopsis</i> and a glucuronic acid-deficient mutant (<i>atdgux</i>). The genetically modified xylan substrates were subsequently utilized to prepare nanocrystals and Pickering emulsions. Notably, nanocrystals derived from lower-substituted xylan displayed more ordered arrangements and higher crystallinity, while conventional xylan substrates exhibited superior emulsifying properties. Furthermore, the modification of xylan side chains significantly influenced the alignment and crystallinity of cellulose microfibrils, enhancing biomass saccharification and reducing cellulose nanocrystal dimensions. This study thus illustrates an effective strategy for achieving diverse and valuable bioproduction through precise genetic engineering of xylan in plants and provides valuable insights into the dynamic mediation of xylan and the construction of cell wall networks.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12874745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146042364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying metabolic bottlenecks for micafungin precursor production via untargeted regulatory perturbation","authors":"Ping Men,&nbsp;Li Xie,&nbsp;Jiachen Wang,&nbsp;Yu Zhou,&nbsp;Xiaoxi Zhang,&nbsp;Yanping Li,&nbsp;Xuenian Huang,&nbsp;Xuefeng Lu","doi":"10.1186/s13068-026-02737-7","DOIUrl":"10.1186/s13068-026-02737-7","url":null,"abstract":"<div><h3>Background</h3><p>Micafungin, a clinically important echinocandin antifungal agent, is derived from the nonribosomal cyclic hexapeptide FR901379 produced by the filamentous fungus <i>Coleophoma empetri</i>. However, low fermentation efficiency remains a major constraint in its industrial production.</p><h3>Results</h3><p>In this study, we implemented an untargeted regulatory perturbation strategy to systematically identify metabolic bottlenecks affecting FR901379 biosynthesis. A mutant library was constructed by rationally engineering the key untargeted regulatory genes involved in histone modification and global regulation. The untargeted perturbation led to diverse phenotypes in both growth and secondary metabolism, ranging from enhancement (by up to 170%) to complete abolition of FR901379 production. Transcriptome profiling of high-producing strains revealed coordinated upregulation of genes in the acetyl-CoA, palmitic acid, and 3′-phosphoadenosine-5′-phosphosulfate biosynthetic pathways. Exogenous supplementation of palm oil further enhanced FR901379 titer by 87.6%, confirming the critical role of precursor supply.</p><h3>Conclusions</h3><p>This work elucidates the metabolic network governing FR901379 biosynthesis and provides key candidates for further metabolic engineering. It also demonstrates that untargeted regulatory perturbation strategy is an effective approach for deciphering the mechanisms behind specific phenotypic traits in industrial filamentous fungi.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12874660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145971671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and design of novel glucoamylases suitable for raw starch hydrolysis at moderate temperatures via an integrated bioinformatics-assisted strategy","authors":"Shuangshuang Ge,&nbsp;Yimeng Song,&nbsp;Tong Ye,&nbsp;Xueting Zhang,&nbsp;Xuecheng Zhang,&nbsp;Yinliang Zhang,&nbsp;Yazhong Xiao,&nbsp;Wei Fang","doi":"10.1186/s13068-025-02725-3","DOIUrl":"10.1186/s13068-025-02725-3","url":null,"abstract":"<div><p>Glucoamylases capable of hydrolyzing raw starch at moderate temperatures exhibit significant potential for the direct and efficient hydrolysis of raw starch. Using bioinformatics-assisted mining strategies, four candidate glucoamylases were initially identified from the GenBank database. Among these candidates, SeGA demonstrated high specific activities of 412.94 U/mg toward soluble starch and 143.51 U/mg toward raw corn starch. The half-life of SeGA was 192 h at 40 °C. Using SeGA as the initial enzyme, FuncLib design was performed to enhance its activity. The mutant SeGA-21 exhibited higher specific activity of 466.22 U/mg toward soluble starch and 173.98 U/mg toward raw corn starch, representing increases of 1.13-fold and 1.21-fold compared to SeGA, respectively. The half-life of SeGA-21 at 40 °C increased to 240 h, representing a 25% increase compared with SeGA. SeGA-21 can efficiently hydrolyze 30% raw corn starch, achieving a hydrolysis rate of approximately 31% after a 9-h reaction. When working synergistically with amylolytic enzymes, a hydrolysis rate of 71.5% was achieved for 30% raw corn starch at 40 °C within 15 h of reaction. Due to its ability to hydrolyze raw starch, moderate reaction temperature requirements, and excellent thermostability, SeGA-21 can be regarded as an efficient enzyme for the cold hydrolysis of raw starch and starch modification.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12874731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving inhibitor tolerance of xylitol-producing Saccharomyces cerevisiae by overexpressing key target genes mined through comparative transcriptomes","authors":"Xin-Yu Xiao,&nbsp;Xin-Yu Wang,&nbsp;Ya-Jing Wu,&nbsp;Ying Cheng,&nbsp;Quan Zhang,&nbsp;Cai-Yun Xie,&nbsp;Yue-Qin Tang","doi":"10.1186/s13068-026-02735-9","DOIUrl":"10.1186/s13068-026-02735-9","url":null,"abstract":"<div><h3>Background</h3><p>Xylitol, a valuable five-carbon sugar alcohol widely used in the food and pharmaceutical industries, can be biosynthesized through the reduction of xylose by engineered <i>Saccharomyces cerevisiae</i>. A major challenge in producing xylitol from lignocellulosic feedstocks is the sensitivity of yeast to multiple inhibitors generated during biomass pretreatment. Developing robust microbial cell factories with enhanced tolerance to these inhibitors is therefore essential for efficient and sustainable xylitol production. In this study, we employed comparative transcriptomic analysis to investigate the response mechanisms of two xylitol-producing <i>S. cerevisiae</i> strains, CXAU and TX2022, to vanillin and PCS-L (liquid hydrolysate from pretreated corn stover).</p><h3>Results</h3><p>Under vanillin stress, CXAU exhibited downregulation of glycolysis, the pentose phosphate pathway (PPP), and the tricarboxylic acid (TCA) cycle, accompanied by upregulation of amino acid and ergosterol biosynthesis. In contrast, TX2022 showed repression of central carbon metabolism, oxidative phosphorylation, and heme and thiamine synthesis, while enhancing amino acid synthesis and glutathione (GSH) regeneration. Under PCS-L exposure, CXAU experienced severe metabolic disruption but prioritized improving the fidelity of protein translation. Meanwhile, TX2022 upregulated amino acid and ergosterol synthesis, purine metabolism, and ribosome biogenesis, while downregulating oxidative phosphorylation and peroxisomal functions. Based on transcriptomic insights, 11 candidate genes potentially involved in stress tolerance were identified and individually overexpressed. Overexpression of <i>SIP18</i> or <i>CTT1</i> significantly enhanced tolerance to both vanillin and complex inhibitors. Additionally, overexpression of <i>AAD4</i> or <i>AAD6</i> improved vanillin tolerance, whereas <i>SPI1</i> or <i>GRE1</i> overexpression conferred increased resistance to the complex inhibitors. Notably, the engineered strain TX2022-SIP18 achieved high-level xylitol production of 43.50 g/L (yield: 0.961 g/g xylose) in concentrated hydrolysate from pretreated corn cob containing high concentrations of inhibitors.</p><h3>Conclusions</h3><p>This study provides the first experimental evidence that <i>SIP18</i>, <i>AAD4</i>, <i>AAD6</i>, <i>SPI1</i>, <i>CTT1</i>, and <i>GRE1</i> contribute to inhibitor tolerance of <i>S. cerevisiae</i>, highlighting their potential as targets for engineering robust industrial strains for sustainable lignocellulosic xylitol production.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12874835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145949533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omic and phenotypic analysis of open raceway pond production of Monoraphidium minutum 26B-AM reveals distinct stress signatures for scale-up and infection","authors":"Georgios Kepesidis,&nbsp;Jenna Schambach,&nbsp;Daniel Yang,&nbsp;Elise Wilbourn,&nbsp;Thomas Sheffield,&nbsp;Tyler Eckles,&nbsp;Olivia Watt,&nbsp;Matthew P. Hirakawa,&nbsp;Todd W. Lane,&nbsp;Raga Krishnakumar","doi":"10.1186/s13068-025-02730-6","DOIUrl":"10.1186/s13068-025-02730-6","url":null,"abstract":"<div><h3>Background</h3><p>Green microalgae, such as <i>Monoraphidium minutum</i> 26B-AM, have garnered significant commercial interest due to their high biomass production and lipid yield, providing promising candidates for various bioprocessing applications. However, the economic viability of large-scale algal cultivation in open raceway ponds is limited by biocontamination and environmental stressors, necessitating deeper understanding of the molecular mechanisms that underpin resilience and productivity in these systems. We hypothesized that the molecular signature associated with the cellular responses of <i>M. minutum</i> to environmental stressors will reveal critical information for the timely prediction of resilience and productivity in algal cultures within open pond systems.</p><h3>Results</h3><p>To test this hypothesis, we conducted a longitudinal multi-omic study, integrating transcriptomics, proteomics, metabolomics, and phenomics, to monitor the acclimation, growth dynamics, and pathogen responses of algal cultures in two 1000 L raceway ponds, before and after the introduction of a pathogen as a stressor. We identified a number of molecular patterns that correlate with changes in the algal environment, and we can track these changes within the ponds per time. Furthermore, we identify scale-up and infection-specific molecular pathways through integrated multi-omics, showing that most patterns are unique to each studied stressor/transition.</p><h3>Conclusions</h3><p>Ultimately, this study demonstrates the utility of multi-omics observations at scale, revealing unique signatures and laying the groundwork for developing molecular detection techniques and predictive models that can improve the sustainability and efficiency of large-scale algae biomass production.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13068-025-02730-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145936169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms","authors":"Lara Strehl,&nbsp;Paul Richter,&nbsp;Jathurshan Panchalingam,&nbsp;Robert Dinger,&nbsp;Franziska Höfele,&nbsp;Frank R. Bengelsdorf,&nbsp;Marcel Mann","doi":"10.1186/s13068-025-02732-4","DOIUrl":"10.1186/s13068-025-02732-4","url":null,"abstract":"<div><p>Gas fermentation offers a sustainable alternative for valorizing climate-active gases and industrial off-gases. Currently, these gases require energy-intensive purification steps before they can be used in chemical processes such as Fischer–Tropsch synthesis. In gas fermentation, anaerobic bacteria produce acetate from industrial off-gases. Compared to chemical processes, the anaerobic bacteria offer greater tolerance to varying gas concentrations and impurities. One major product of these anaerobic valorization processes is acetate, which can be used as a co-substrate in a variety of biological processes. This study evaluates <i>Corynebacterium glutamicum</i> and <i>Ustilago maydis</i> in benchtop cultivations using 10–20% (v/v) sterile-filtered acetate-rich supernatants from <i>Acetobacterium woodii</i> fermentation to produce L-lysine and triglycerides. Partial substitution of glucose with these supernatants supported robust growth and required no additional purification beyond sterile filtration. <i>C. glutamicum</i> achieved a L-lysine concentration of 3.5 ± 0.27 g∙L⁻¹ and exhibited a diauxic growth pattern on glucose and acetate. In <i>U. maydis</i>, supernatant addition shortened the lag phase by approximately 2 h but reduced triglyceride yields modestly due to higher nitrogen availability. Optimizing the nitrogen-to-carbon ratio in benchtop fermentations resulted in a triglyceride concentration of 12.75 ± 1.17 g∙L⁻¹, demonstrating the feasibility of this approach. Collectively, the results demonstrate a viable method for replacing a portion of refined glucose with acetate-rich supernatants, thereby enabling a cost-efficient integration of anaerobic gas valorization with aerobic biomanufacturing.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13068-025-02732-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145947001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of lignin and food waste into vanillin and biohydrogen by Escherichia coli MC3: a novel dual-functional bacteria","authors":"Dongye Chuancheng,&nbsp;Zhihe Li,&nbsp;Waqar Iqbal,&nbsp;Andong Zhang,&nbsp;Hesham M. Hassan,&nbsp;Ahmed Al-Emam,&nbsp;Tawaf Ali Shah","doi":"10.1186/s13068-025-02728-0","DOIUrl":"10.1186/s13068-025-02728-0","url":null,"abstract":"<div><p>This study introduces <i>Escherichia coli</i> MC3, demonstrating unique dual functionality in coupling lignin depolymerization with biohydrogen production from untreated food waste, thereby advancing sustainable biorefineries without energy-intensive pretreatments. MC3 demonstrated a lignin degradation rate of 50.3 ± 3.2% and an Azure B decolorization rate of 34.8 ± 2.1% over a 7-day period, as validated by UV–visible spectrophotometry. High-performance liquid chromatography identified ferulic acid (2.355 mg/L) and vanillin (1.018 mg/L) as primary byproducts of lignin degradation, thereby confirming its ligninolytic potential. Enzymatic assays revealed significant hydrolytic activity, with hydrolysis zones measuring 25.4 ± 1 mm (proteases), 28.1 ± 2 mm (cellulases), 20.5 ± 3 mm (xylanases), and 6.8 ± 1 mm (amylases). Whole-genome sequencing of the <i>E. coli</i> strain MC3 revealed a total of 4798 coding sequences. Among these, key genes identified include carbohydrate-active enzymes (CAZymes: AA2, AA3, CE1), hydrolytic enzymes (GH2, GH13, GH8), multicopper oxidases (CueO, CopA), and biohydrogen-related enzymes (NikA, FbpC). These findings highlight the potential of MC3 for lignin degradation and hydrogen production. In batch fermentation assays, the strain demonstrated a hydrogen yield ranging from 0.292 to 1.004 mol H2 per mol of substrate across various substrates, including xylose, glucose, carboxymethyl cellulose, starch, and food waste, with the highest yield obtained from food waste. The <i>E. coli</i> strain MC3 produced the highest cumulative hydrogen yield of 175 ± 10 mL H₂/g VS from food waste. This study confirms the dual function of <i>E. coli</i> strain MC3 for conversion of lignin into vanillin and biomass into biohydrogen, suggesting the excluding energy-intensive pretreatments, substantial potential for sustainable biorefineries.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13068-025-02728-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145947073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioenergy carbon capture storage and utilization: a critical review of market dynamics and policy implications","authors":"Dig Vijay Singh,&nbsp;Senthil Nagappan,&nbsp;Chyi-how Lay,&nbsp;Bartłomiej Igliński,&nbsp;Grzegorz Piechota,&nbsp;Gopalakrishnan Kumar,&nbsp;Roberto Parra Saldivar,&nbsp;Vinod Kumar","doi":"10.1186/s13068-025-02724-4","DOIUrl":"10.1186/s13068-025-02724-4","url":null,"abstract":"<div><p>Bioenergy with carbon capture, utilization, and storage (BECCUS) is a competent technology with the potential to address global climate change challenges. However, its deployment faces significant hurdles across technological, economic, and policy domains. The production of biofuels including ethanol, methane, butanol, and biogas is accompanied by the release of carbon dioxide (CO₂). This CO₂ can be incorporated into organic molecules through various biochemical routes as part of the metabolic mechanisms of carbon absorption. The efficiency of these carbon assimilation pathways can be improved through ongoing developments in metabolic engineering, which can increase the production of valuable bioproducts, improve carbon sequestration, and support efforts to mitigate climate change. The present review recognizes critical avenues for advancing BECCUS, emphasizing market mechanisms, technological innovations, and cross-sector integration in both developed and developing countries such as India. The review recommends policy modifications aimed at establishing a transparent framework related to carbon pricing, emission trading systems, and proper certification mechanisms for biogenic carbon utilization. These modifications, coupled with the integration of renewable energy systems, would not only stimulate BECCUS adoption, but also foster its economic feasibility and sustainability. Additionally, promising technologies such as chemical looping and microalgae-based carbon capture should be technologically scaled up to ensure industrial-level applications. The integration of BECCUS with other sectors is also critical to optimize the impact of this technology on climate change mitigation. Therefore, the present review highlights the need for a robust policy framework, technology-driven innovation, and cross-sector research collaboration to resolve the challenges associated with BECCUS, boost its adoption, and ensure its economic feasibility and environmental sustainability. Moreover, providing regulatory support, augmenting market competitiveness, and aligning research on BECCUS play a transformative role in attaining the goals of the Paris Agreement and promoting environmental sustainability.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13068-025-02724-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145936736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability","authors":"Jiaming Chen,&nbsp;Jian Hao,&nbsp;Frank Baganz","doi":"10.1186/s13068-025-02720-8","DOIUrl":"10.1186/s13068-025-02720-8","url":null,"abstract":"<div><p>Isobutanol holds significant potential as a next-generation biofuel and platform chemical, offering a viable alternative to petroleum across various industries, including pharmaceuticals, and fine chemicals. Biosynthesis has emerged as a highly advantageous approach for isobutanol production, surpassing conventional chemical synthesis methods in terms of environmental sustainability, safety, and ecological compatibility. Consequently, the field of isobutanol biosynthesis has garnered increasing interest. This review provides an overview of recent breakthroughs in isobutanol biosynthesis and explores strategies aimed at improving its production yield from both biological and engineering perspectives. On the biological front, the exploration and optimization of enzymatic pathways, metabolic engineering techniques to enhance precursor availability and flux, and cellular engineering approaches to improve strain tolerance and production efficiency are meticulously analysed. Additionally, engineering aspects such as in situ product removal methods and biomass fermentation are introduced, as they significantly contribute to enhancing yields and economy. Despite the immense promise of isobutanol biosynthesis, several challenges require attention. The modification of enzymatic and metabolic pathways is crucial for increasing production efficiency and yield. The adoption of high-throughput screening techniques becomes indispensable for identifying and selecting optimal strains and enzymes with improved characteristics. Furthermore, the cytotoxic nature and volatility of isobutanol pose additional hurdles, leading to losses during the production process. By effectively addressing these challenges and advancing our understanding of isobutanol biosynthesis, we can pave the way for more efficient and sustainable production methods. This review aims to provide valuable insights and inspiration to researchers in the field, with the ultimate objective of accelerating the development and application of isobutanol biosynthesis.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s13068-025-02720-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}