通过将异源鼠李糖基转移酶基因导入弹尾星菌,生产新型/嵌合型低聚糖-鼠李糖生物表面活性剂并确定其特性。

IF 6.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mingxin Liu, Tianshuang Tu, Hui Li, Xin Song
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引用次数: 0

摘要

糖脂类生物表面活性剂、槐糖脂(SLs)和鼠李糖脂(RLs)可广泛应用于农业、食品和化学工业。SLs和RLs不同的理化性质,如亲水亲脂值(HLB)和临界胶束浓度(CMC),决定了它们有不同的应用重点。研究人员仍希望获得具有独特表面活性的新型糖脂表面活性剂。在这项研究中,我们成功地将铜绿假单胞菌中的两个鼠李糖基转移酶基因 rhlA 和 rhlB 转化到能产生槐脂的星形菌 CGMGG 1576 中,得到了重组菌株 SbrhlAB。用 ASB C18 色谱柱从 SbrhlAB 的发酵液中鉴定出两种分子量分别为 554(C26H50O12)和 536(C26H48O11)的新成分,前者是含有一个葡萄糖和一个鼠李糖的非乙酰化酸性 C14:0 糖脂,后者是含有两个鼠李糖的酸性 C14:1 糖脂。用 Venusil MP C18 色谱柱鉴定出了一种新的糖脂成分,即含有一个鼠李糖(C24H40O7)的酸性 C18:3 糖脂,这在以前从未报道过。本研究表明,新型糖脂可通过合理的基因工程在体内合成。这些研究结果将有助于以更合理、更可控的方式改造生产糖脂的酵母,以生产一些特定的糖脂或其衍生物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production and characterization of novel/chimeric sophorose–rhamnose biosurfactants by introducing heterologous rhamnosyltransferase genes into Starmerella bombicola

Glycolipid biosurfactant, sophorolipids (SLs) and rhamnolipids (RLs) can be widely used in agriculture, food and chemical industries. The different physicochemical properties of SLs and RLs, such as hydrophilic lipophilic value (HLB) and critical micelle concentration (CMC), determine they have different application focus. Researchers are still hoping to obtain new glycolipid surfactants with unique surface activities. In this study, we successfully transformed two rhamnosyltransferase genes rhlA and rhlB from Pseudomonas aeruginosa to the sophorolipid-producing Starmerella bombicola CGMGG 1576 to obtain a recombinant strain was SbrhlAB. Two novel components with molecular weight of 554 (C26H50O12) and 536 (C26H48O11) were identified with the ASB C18 column from the fermentation broth of SbrhlAB, the former was a non-acetylated acidic C14:0 glycolipid containing one glucose and one rhamnose, and the latter was an acidic C14:1 glycolipid containing two rhamnoses. With the Venusil MP C18 column, one new glycolipid component was identified as an acidic C18:3 glycolipid with one rhamnose (C24H40O7), which has not been reported before. Our present study demonstrated that novel glycolipids can be synthesized in vivo by reasonable genetic engineering. The results will be helpful to engineer sophorolipid-producing yeast to produce some specific SLs or their derivatives in more rational and controllable way.

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来源期刊
Biotechnology for Biofuels
Biotechnology for Biofuels 工程技术-生物工程与应用微生物
自引率
0.00%
发文量
0
审稿时长
2.7 months
期刊介绍: Biotechnology for Biofuels is an open access peer-reviewed journal featuring high-quality studies describing technological and operational advances in the production of biofuels, chemicals and other bioproducts. The journal emphasizes understanding and advancing the application of biotechnology and synergistic operations to improve plants and biological conversion systems for the biological production of these products from biomass, intermediates derived from biomass, or CO2, as well as upstream or downstream operations that are integral to biological conversion of biomass. Biotechnology for Biofuels focuses on the following areas: • Development of terrestrial plant feedstocks • Development of algal feedstocks • Biomass pretreatment, fractionation and extraction for biological conversion • Enzyme engineering, production and analysis • Bacterial genetics, physiology and metabolic engineering • Fungal/yeast genetics, physiology and metabolic engineering • Fermentation, biocatalytic conversion and reaction dynamics • Biological production of chemicals and bioproducts from biomass • Anaerobic digestion, biohydrogen and bioelectricity • Bioprocess integration, techno-economic analysis, modelling and policy • Life cycle assessment and environmental impact analysis
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