Biotechnology for Biofuels最新文献

{"title":"Involvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes’ synthesis","authors":"Zhen Zhang,&nbsp;Hua Li,&nbsp;Feiyu Dong,&nbsp;Hui Lin,&nbsp;Yanan Li,&nbsp;Kun Cheng,&nbsp;Hongge Chen","doi":"10.1186/s13068-025-02610-z","DOIUrl":"10.1186/s13068-025-02610-z","url":null,"abstract":"<div><h3>Background</h3><p><i>Aspergillus niger</i> is an important lignocellulose-degrading enzyme-producing strain. Multiple regulatory factors regulate the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>. We previously found that <i>A. niger</i> possessed an intracellular β-glucosidase BGL1B, and the intracellular localization of BGL1B and its active transglycosylation action prompted us to explore whether BGL1B was involved in the regulation of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>.</p><h3>Results</h3><p>In this study, by investigating the production of lignocellulose-degrading enzymes of <i>bgl1B</i> knockout strain (Δ<i>bgl1B</i>) and overexpression strain (OE::<i>bgl1B</i>), it was found that BGL1B exhibited a repressive role on the expression of lignocellulose-degrading enzyme genes through carbon catabolite repression (CCR) way. On the other hand, BGL1B’s transglycosylation products sophorose and laminaribiose were proved to be able to induce the expression of lignocellulose-degrading enzyme genes, which explained why OE::<i>bgl1B</i> showed the same enhanced enzyme activity and gene expression as Δ<i>bgl1B</i> strain compared to the starting strain (WT).</p><h3>Conclusions</h3><p>The present study demonstrates that BGL1B plays dual regulatory roles in the regulation of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>: the repressive role caused by BGL1B’s hydrolysis product glucose and the induction role caused by BGL1B’s transglycosylation products sophorose and laminaribiose. This study broadens the understanding of the regulatory network of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>. Also, it provides a strategy to create an engineered strain with high production of lignocellulose-degrading enzymes.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shewanella oneidensis and Methanosarcina barkerii augmentation and conductive material effects on long-term anaerobic digestion performance","authors":"Perego Camilla,&nbsp;König Roger,&nbsp;Cuomo Maurizio,&nbsp;Pianta Elisa,&nbsp;Sunny Maye,&nbsp;Loredana Di Maggio,&nbsp;Michel Moser,&nbsp;Fischer Fabian,&nbsp;Principi Pamela","doi":"10.1186/s13068-025-02609-6","DOIUrl":"10.1186/s13068-025-02609-6","url":null,"abstract":"<div><p>This study explores the use of conductive material in scaling up anaerobic digestion for enhanced biogas production. Focusing on Direct Interspecies Electron Transfer (DIET), the research employs a syntrophic DIET-able consortium formed by <i>Shewanella oneidensis</i> and <i>Methanosarcina barkerii</i> in 3.8-L experiments utilizing reticulated vitreous carbon (RVC) as conductive material. In short-term tests with acetate the syntrophic co-culture with RVC resulted in 86% higher maximum velocity of methane production, while in long term with real feed 13% increased rate was observed: the addition of 1.77 (S/m)*m² RVC resulted in a faster methane production of 2.39 mL/gVS*h compared to 2.08 mL/gVS*h of the reference. The experimental conditions of syntrophic inoculum and RVC as conductive material gave a benefit in terms of process rate compared to the reference, considering the inoculum fate, <i>Methanosarcina barkerii</i> was among the dominant taxa at the end of the experiment, while <i>Shewanella oneidensis</i> was outcompeted. Among the methanogenesis production pathways, an increase of hydrogenotrophic methanogenesis has been observed in presence of conductive material. Further research is needed to understand the role of RVC in sulfur compounds production. Utilization of RVC to augment methane production yielded interesting results for real-scale application. As an added carrier, RVC remains unaltered and can be readily recuperated and reused multiple times.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02609-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metagenomics reveals the metabolic flexibility of coastal prokaryotic microbiomes contributing to lignin degradation","authors":"Qiannan Peng,&nbsp;Lu Lin","doi":"10.1186/s13068-025-02605-w","DOIUrl":"10.1186/s13068-025-02605-w","url":null,"abstract":"<div><p>Coastal wetlands are rich in terrestrial organic carbon. Recent studies suggest that microbial consortia play a role in lignin degradation in coastal wetlands, where lignin turnover rates are likely underestimated. However, the metabolic potentials of these consortia remain elusive. This greatly hinders our understanding of the global carbon cycle and the “bottom-up” design of synthetic consortia to enhance lignin conversion. Here, we developed two groups of lignin degrading consortia, L6 and L18, through the 6- and 18-month in situ lignin enrichments in the coastal East China Sea, respectively. Lignin degradation by L18 was 3.6-fold higher than L6. Using read-based analysis, 16S rRNA amplicon and metagenomic sequencing suggested that these consortia possessed varied taxonomic compositions, yet similar functional traits. Further comparative metagenomic analysis, based on metagenomic assembly, revealed that L18 harbored abundant metagenome-assembled genomes (MAGs) that encoded diverse and unique lignin degradation gene clusters (LDGCs). Importantly, anaerobic MAGs were significantly enriched in L18, highlighting the role of anaerobic lignin degradation. Furthermore, the generalist taxa, which possess metabolic flexibility, increased during the extended enrichment period, indicating the advantage of generalists in adapting to heterogenous resources. This study advances our understanding of the metabolic strategies of coastal prokaryotic consortia and lays a foundation for the design of synthetic communities for sustainable lignocellulose biorefining.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02605-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing biomass and lipid productivities of Haematococcus pluvialis for industrial raw materials products","authors":"Övgü Gencer,&nbsp;Gamze Turan","doi":"10.1186/s13068-025-02604-x","DOIUrl":"10.1186/s13068-025-02604-x","url":null,"abstract":"<div><p>For biofuels and nutraceuticals, the green microalga Haematococcus pluvialis (Chlorophyceae) is a prospective source of biomass and lipids. This study examined how biomass production and lipid accumulation were affected by temperature (10 °C, 20 °C, and 30 °C) and potassium nitrate (KNO₃) concentrations (0.41 g/L, 0.31 g/L, 0.21 g/L, 0.10 g/L, and 0). The findings showed that the largest biomass (0.665 ± 0.200 g/L) was produced at a potassium nitrate concentration of 0.21 g/L at 20 °C, whereas the highest lipid content (46.31 ± 0.026% dry weight) was produced at a temperature without nitrate. Notably, a balanced result was obtained with a modest nitrate content (0.10 g/L) at 20 °C, yielding significant biomass (0.560 ± 0.136 g/L) and lipids (40.30 ± 0.012% dry weight). These results highlight how crucial it is to optimize cultivation settings in order to increase H. pluvialis's dual productivity, offering important new information for its industrial-scale use. By adjusting growing conditions, this research helps meet the need for renewable resources worldwide by promoting the production of high-value bioproducts and sustainable, commercially viable algae-based biofuels.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02604-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production","authors":"Mostafa E. Elshobary,&nbsp;Walaa A. Abo-Shanab,&nbsp;Stephan S. W. Ende,&nbsp;Mohammed Alquraishi,&nbsp;Rania A. El-Shenody","doi":"10.1186/s13068-024-02602-5","DOIUrl":"10.1186/s13068-024-02602-5","url":null,"abstract":"<div><h3>Background</h3><p><i>Phaeodactylum tricornutum</i> is a versatile marine microalga renowned for its high-value metabolite production, including omega-3 fatty acids and fucoxanthin, with emerging potential for integrated biorefinery approaches that encompass biofuel and bioproduct generation. Therefore, in this study we aimed to optimize the cultivation conditions for boosting biomass, lipid, and fucoxanthin production in <i>P. tricornutum</i>, focusing on the impacts of different nutrient ratios (nitrogen, phosphorus, silicate), glycerol supplementation, and light regimes.</p><h3>Results</h3><p>Optimized medium (− 50%N%, + 50% P, Zero-Si, 2 g glycerol) under low-intensity blue light (100 μmol m⁻² s⁻¹) improved biomass to 1.6 g L⁻¹, with lipid productivity reaching 539.25 mg g⁻¹, while fucoxanthin increased to 20.44 mg g⁻¹. Total saturated fatty acid (ΣSFA) content in the optimized culture increased approximately 2.4-fold compared to the control F/2 medium. This change in fatty acid composition led to improved biodiesel properties, including a higher cetane number (59.18 vs. 56.04) and lower iodine value (53.96 vs 88.99 g I₂/100 g oil). The optimized conditions also altered the biodiesel characteristics, such as kinematic viscosity, cloud point, and higher heating value.</p><h3>Conclusion</h3><p>Our optimization approach reveals the significant potential of <i>P. tricornutum</i> as a versatile microbial platform for biomass, lipid, and fucoxanthin production. The tailored cultivation strategy successfully enhanced biomass and lipid accumulation, with notable improvements in biodiesel properties through strategic nutrient and light regime manipulation. These findings demonstrate the critical role of precise cultivation conditions in optimizing microalgal metabolic performance for biotechnological applications.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02602-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A parallel bioreactor strategy to rapidly determine growth-coupling relationships for bioproduction: a mevalonate case study","authors":"Alec Banner,&nbsp;Joseph Webb,&nbsp;Nigel Scrutton","doi":"10.1186/s13068-024-02599-x","DOIUrl":"10.1186/s13068-024-02599-x","url":null,"abstract":"<div><h3>Background</h3><p>The climate crisis and depleting fossil fuel reserves have led to a drive for ‘green’ alternatives to the way we manufacture chemicals, and the formation of a bioeconomy that reduces our reliance on petrochemical-based feedstocks. Advances in Synthetic biology have provided the opportunity to engineer micro-organisms to produce compounds from renewable feedstocks, which could play a role in replacing traditional, petrochemical based, manufacturing routes. However, there are few examples of bio-manufactured products achieving commercialisation. This may be partially due to a disparity between academic and industrial focus, and a greater emphasis needs to be placed on economic feasibility at an earlier stage. Terpenoids are a class of compounds with diverse use across fuel, materials and pharmaceutical industries and can be manufactured biologically from the key intermediate mevalonate.</p><h3>Results</h3><p>Here, we report on a method of utilising parallel bioreactors to rapidly map the growth-coupling relationship between the specific product formation rate, specific substrate utilisation rate and specific growth rate. Using mevalonate as an example product, a maximum product yield coefficient of 0.18 g_p/g_s was achieved at a growth rate (<span>(mu)</span>) of 0.34 h⁻¹. However, this process also led to the formation of the toxic byproduct acetate, which can slow growth and cause problems during downstream processing. By using gene editing to knock out the <i>ackA-pta</i> operon and <i>poxB</i> from <i>E. coli</i> BW25113, we were able to achieve the same optimum production rate, without the formation of acetate.</p><h3>Conclusions</h3><p>We demonstrated the power of using parallel bioreactors to assess productivity and the growth-coupling relationship between growth rate and product yield coefficient of mevalonate production. Using genetic engineering, our resultant strain demonstrated rapid mevalonate formation without the unwanted byproduct acetate. Mevalonate production is quantified and reported in industrially relevant units, including key parameters like conversion efficiency that are often omitted in early-stage publications reporting only titre in g/L.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02599-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-level biosynthesis and purification of the antimicrobial peptide Kiadin based on non-chromatographic purification and acid cleavage methods","authors":"Liangjun Zheng,&nbsp;Fengyi Yang,&nbsp;Chen Wang,&nbsp;Muhammad Zafir,&nbsp;Zishuo Gao,&nbsp;Pilong Liu,&nbsp;Fatma A. El-Gohary,&nbsp;Xin Zhao,&nbsp;Huping Xue","doi":"10.1186/s13068-025-02607-8","DOIUrl":"10.1186/s13068-025-02607-8","url":null,"abstract":"<div><p>Antimicrobial peptides (AMPs) are renowned for their potent bacteriostatic activity and safety, rendering them invaluable in animal husbandry, food safety, and medicine. Despite their potential, the physiological toxicity of AMPs to host cells significantly hampers their biosynthetic production. This study presents a novel approach for the biosynthesis of the antimicrobial peptide Kiadin by engineering a DAMP4–DPS–Kiadin fusion protein to mitigate host cell toxicity and achieve high-level expression. Leveraging the unique properties of the DAMP4 protein, we developed a non-chromatographic purification method to isolate the DAMP4–DPS–Kiadin fusion protein with high purity. The instability of the D–P peptide bond under acidic conditions, combined with the thermal and saline stability of DAMP4, enabled efficient separation of Kiadin through acid cleavage and isoelectric precipitation, yielding Kiadin with 96% purity and a production yield of 29.3 mg/L. Our optimization of acid cleavage temperature, duration, and isoelectric precipitation conditions proved critical for maximizing the purification efficiency and expression levels of Kiadin. The biosynthesized Kiadin exhibited robust bacteriostatic activity against <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, <i>Bacillus cereus</i> and <i>Staphylococcus aureus</i>. Notably, Kiadin demonstrated significant post-antibiotic effects by disrupting bacterial membrane integrity, inducing cytoplasmic leakage, and inhibiting biofilm formation in <i>E. coli</i> K88 and <i>S. aureus</i> Mu50, without cytotoxicity towards mouse macrophages. In vivo studies further confirmed Kiadin's exceptional therapeutic efficacy against abdominal infections caused by <i>E. coli</i> K88. The acid cleavage and non-chromatographic purification techniques developed in this study offer a cost-effective and efficient strategy for the high-purity production of AMPs.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02607-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Bubbling insights: unveiling the true sophorolipid biosynthetic pathway by Starmerella bombicola","authors":"Sophie L. K. W. Roelants,&nbsp;Stijn Bovijn,&nbsp;Elvira Bytyqi,&nbsp;Nicolas de Fooz,&nbsp;Goedele Luyten,&nbsp;Martijn Castelein,&nbsp;Thibo Van de Craen,&nbsp;Zhoujian Diao,&nbsp;Karolien Maes,&nbsp;Tom Delmulle,&nbsp;Maarten De Mol,&nbsp;Sofie L. De Maeseneire,&nbsp;Bart Devreese,&nbsp;Wim K. Soetaert","doi":"10.1186/s13068-024-02600-7","DOIUrl":"10.1186/s13068-024-02600-7","url":null,"abstract":"","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02600-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Batch and semi-continuous fermentation with Parageobacillus thermoglucosidasius DSM 6285 for H2 production","authors":"Magda S. Ardila,&nbsp;Habibu Aliyu,&nbsp;Pieter de Maayer,&nbsp;Anke Neumann","doi":"10.1186/s13068-024-02597-z","DOIUrl":"10.1186/s13068-024-02597-z","url":null,"abstract":"<div><h3>Background</h3><p><i>Parageobacillus thermoglucosidasius</i> is a facultatively anaerobic thermophile that is able to produce hydrogen (H₂) gas from the oxidation of carbon monoxide through the water–gas shift reaction when grown under anaerobic conditions. The water–gas shift (WGS) reaction is driven by a carbon monoxide dehydrogenase–hydrogenase enzyme complex. Previous experiments exploring hydrogenogenesis with <i>P. thermoglucosidasius</i> have relied on batch fermentations comprising defined media compositions and gas atmospheres. This study evaluated the effects of a semi-continuous feeding strategy on hydrogenogenesis.</p><h3>Results</h3><p>A batch and two semi-continuous fermentations, with feeding of the latter fresh media (with glucose) in either 24 h or 48 h intervals were undertaken and H₂ production, carbon monoxide dehydrogenase (CODH) activity, and metabolite consumption/production were monitored throughout. Maximum H₂ production rates (HPR) of 0.14 and 0.3 mmol min⁻¹, were observed for the batch and the semi-continuous fermentations, respectively. Daily feeding attained stable H₂ production for 7 days, while feeding every 48 h resulted in high variations in H₂ production. CODH enzyme activity correlated with H₂ production, with a maximum of 1651 U mL⁻¹ on day 14 with the 48 h feeding strategy, while CODH activity remained relatively constant throughout the fermentation process with the 24 h feeding strategy.</p><h3>Conclusions</h3><p>The results emphasize the significance of a semi-continuous glucose-containing feed for attaining stable hydrogen production with <i>P. thermoglucosidasius</i>. The semi-continuous fermentations achieved a 46% higher HPR than the batch fermentation. The higher HPRs achieved with both semi-continuous fermentations imply that this approach could enhance the biohydrogen platform. However, optimizing the feeding interval is pivotal to ensuring stable hydrogen production.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02597-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbohydrate conversion in spent coffee grounds: pretreatment strategies and novel enzymatic cocktail to produce value-added saccharides and prebiotic mannooligosaccharides","authors":"Ali Shaikh-Ibrahim,&nbsp;Nicola Curci,&nbsp;Federica De Lise,&nbsp;Oriana Sacco,&nbsp;Mauro Di Fenza,&nbsp;Stefany Castaldi,&nbsp;Rachele Isticato,&nbsp;André Oliveira,&nbsp;José P. S. Aniceto,&nbsp;Carlos M. Silva,&nbsp;Luísa Seuanes Serafim,&nbsp;Kristian B. R. M. Krogh,&nbsp;Marco Moracci,&nbsp;Beatrice Cobucci-Ponzano","doi":"10.1186/s13068-024-02601-6","DOIUrl":"10.1186/s13068-024-02601-6","url":null,"abstract":"<div><h3>Background</h3><p>Spent coffee grounds (SCG) are the most abundant waste byproducts generated from coffee beverage production worldwide. Typically, these grounds are seen as waste and end up in landfills. However, SCG contain valuable compounds that can be valorized and used in different applications. Notably, they are rich in carbohydrates, primarily galactomannan, arabinogalactan type II, and cellulose. Within the framework of a circular bioeconomy, the targeted degradation of these polysaccharides via a tailored cocktail of carbohydrate-active enzymes offers a promising strategy for producing high-value saccharides from coffee waste.</p><h3>Results</h3><p>In this study, various mild pretreatments were evaluated to increase the enzyme accessibility of SCG-derived biomass, reduce lignin content, and minimize hemicellulose loss. Thermostable enzymes were selected to construct an enzymatic cocktail specifically targeting cellulose and hemicelluloses in pretreated SCGs. The approach used achieved a conversion of 52% of the polysaccharide content to oligo- and monosaccharides, producing 17.4 mg of reducing sugars and 5.1 mg of monosaccharides from 50 mg of SCG. Additionally, microwave pretreatment followed by the application of a thermostable endo β-mannanase resulted in the production of 62.3 mg of mannooligosaccharides from 500 mg of SCG. In vitro experiments demonstrated that the produced mannooligosaccharides exhibited prebiotic activity, promoting the growth and biofilm formation of five probiotic bacterial strains.</p><h3>Conclusions</h3><p>This study highlights an effective strategy for the valorization of SCG polysaccharides through mild pretreatment and customized enzymatic cocktails in a circular bioeconomic context. The production of both monosaccharides and oligosaccharides with prebiotic activity illustrates the versatility and commercial potential of SCG as a substrate for high-value saccharides. Furthermore, the use of mild pretreatment methods and thermostable enzymes minimizes chemical inputs and energy demands, aligning with sustainable processing practices. The ability to selectively target and degrade specific polysaccharides within SCG not only enhances the yield of desirable products, but also preserves key structural components, reducing waste and promoting resource efficiency.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02601-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}