Transfusion and Apheresis Science最新文献

{"title":"The development and evanescence of red blood cell antibodies in a 51-year-old pregnant ovum recipient and her pregnancy outcome","authors":"Wenhao Xu ,&nbsp;Chunkang Chang","doi":"10.1016/j.transci.2025.104204","DOIUrl":"10.1016/j.transci.2025.104204","url":null,"abstract":"<div><div>Management of complications, including hemolytic disease of the fetus and newborn (HDFN) and identifying compatible red blood cell (RBC) for future transfusions, related to multiple RBC alloantibodies in extremely advanced age pregnant donor ovum (DO) recipient is a challenge for transfusion medicine, especially during emergencies when alloantibodies become evanescent. Here we reported a 51-year-old woman underwent three cycles of IVF using embryos from her husband sperm and anonymously DO. She was detected with anti-Ce and anti-Jk^a alloantibodies during the pregnancy, with anti-Jk^a becoming evanescent in the third trimester and reinduced after delivery. She had a postpartum hemorrhage of 1200 ml blood loss and transfused 2 units of 800 ml Rh and Kidd compatible RBC screened by Shanghai Blood Center. The newborn experienced moderate HDFN and was treated with phototherapy and intravenous immune globulin.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104204"},"PeriodicalIF":1.4,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of iron levels and hematological indices among blood donors at the Southern Zonal Blood Center, Accra, Ghana","authors":"Samuel Antwi-Baffour ,&nbsp;Benjamin Tetteh Mensah ,&nbsp;Dorinda Naa Okailey Armah ,&nbsp;Hannah Nana Amene Asiedu ,&nbsp;Lawrence Annison","doi":"10.1016/j.transci.2025.104201","DOIUrl":"10.1016/j.transci.2025.104201","url":null,"abstract":"<div><h3>Background</h3><div>Blood donation is essential for maintaining life-saving supplies, but screening often focuses only on hemoglobin, neglecting iron stores. While hemoglobin recovers quickly, iron takes longer to replenish, putting frequent donors at risk. Even without anemia, low iron can cause fatigue, reduced endurance, and cognitive issues, potentially leading to iron-deficiency anemia. Many programs overlook iron status, leaving regular donors vulnerable to chronic depletion.</div></div><div><h3>Objective</h3><div>The objective of the study was to assess the iron status and haematological indices of categories of blood donors.</div></div><div><h3>Methods</h3><div>This cross-sectional study recruited 350 blood donors of which 146 were first-time donors, 146 repeat donors, and 58 hemoglobin-deferred donors from the Southern Zonal Blood Center, Accra, Ghana. For each participant, about 3 mL of blood sample was obtained to estimate FBC for haematological parameters and serum ferritin concentration for iron status.</div></div><div><h3>Results</h3><div>The study involved 350 blood donors and found a median Hb of 12.4 g/dL and ferritin levels ranging from 11.8 to 500 ng/mL. First-time donors showed a positively skewed Hb distribution, while repeat donors had greater variation with outliers. Significant differences (p &lt; 0.001) in Hb, MCV, MCHC, RDW, and serum ferritin were observed across the groups, though MCH showed no significant difference (p = 0.062). Iron depletion was present in 16.7 % of participants, whilst 8.3 % had iron-deficient erythropoiesis, and 75 % had normal ferritin levels.</div></div><div><h3>Conclusion</h3><div>The findings of the study highlight the differences in iron reserves and hematological parameters among donor categories, emphasizing the impact of donation frequency on iron status. This study provides new evidence from Ghana on how blood donation frequency affects iron reserves and haematological indices.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104201"},"PeriodicalIF":1.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype pattern of factor V and XIII abnormalities in the Iranian population: A meta-analysis","authors":"Sadegh Abbasian ,&nbsp;Mojtaba Pouresmaeili Ravari ,&nbsp;Ali Sahebi ,&nbsp;Fatemeh Mezginejad","doi":"10.1016/j.transci.2025.104200","DOIUrl":"10.1016/j.transci.2025.104200","url":null,"abstract":"<div><h3>Background</h3><div>Hemostatic disorders are significantly associated with morbidity and mortality. Hence, the examination of coagulation abnormalities is a cornerstone of prevention and therapy. This meta-analysis aimed to determine the genotype pattern of Factor V (FV) and XIII (FXIII) abnormalities in the Iranian population. Methods The search strategy was conducted in electronic databases including Web of Science, PubMed, Scopus, SID, ProQuest, and Magiran, covering the period from May 10,1990, to May 10, 2019, following the PRISMA principles. Studies of various designs,including cross-sectional, cohort, experimental, and case-control studies, were included based on specific criteria, without any limitations on gender or language.Results Out of 10,449 research entries, 10,432 were screened, with 132 abstracts and titles analyzed, 23 full articles reviewed, and ultimately 11 studies included in the metaanalysis. The mutation c.1691 G&gt;A: GG (95 % CI: 0.98) had the greatest occurrence rate in FV deficient patients, while the 34Val/Leu mutation (95 % CI: 1.00) was detected as the most prevalent mutation in instances of FXIII insufficiency. Conclusions Specific FV and XIII gene mutations are associated with increased risk for thrombotic conditions. FV deficiency with the c.1691 G&gt;A mutation was prevalent in stroke patients, while FXIII deficiency with the 34Val/Leu mutation was linked to recurrent miscarriages.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104200"},"PeriodicalIF":1.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming ABO IgG titration: Real-world comparison of automated SPRCA vs. CAT with dithiothreitol (DTT) inactivation of IgM in 1600 ABO-incompatible solid organ transplant patient samples","authors":"Amena Ebadur Rahman ,&nbsp;Rasika Dhawan Setia ,&nbsp;Mitu Dogra ,&nbsp;Abhideep Chaudhary ,&nbsp;Ashwini Goel ,&nbsp;Amit Kumar Singhal ,&nbsp;Vadad ,&nbsp;Sonia Joseph ,&nbsp;Priyasha Prajapat","doi":"10.1016/j.transci.2025.104198","DOIUrl":"10.1016/j.transci.2025.104198","url":null,"abstract":"<div><div>Anti-ABO IgG isohemagglutinin(IHA) titration plays a vital role in guiding ABO-incompatible solid organ transplantation(ABOi-SOT). Column agglutination technology (CAT), though widely used, requires DTT pre-treatment to eliminate IgM interference. This poses significant challenges, including subjectivity, inconsistent IgM inactivation, frequent sample gelling, and prolonged turnaround times(TAT), all of which compromise accuracy and standardization. These limitations have prompted global interest in DTT-free, IgG-specific methods that enhance analytical precision and workflow efficiency.</div></div><div><h3>Study Design and Methods</h3><div>This prospective single-centre study (August 2019–October 2024) evaluated 1622 samples from 300 ABOi-SOT candidates; baseline titres done for all; 139 underwent transplantation. IgG titers were assessed via DTT-treated CAT [CAT(DTT)] and SPRCA during desensitization phase and post-transplant monitoring. IHA IgG titers were compared between CAT(DTT) and fully automated solid-phase red cell adherence assay (SPRCA). Concordance &amp; reproducibility was assessed using Pearson’s correlation and Cohen’s kappa and TATs compared.</div></div><div><h3>Results</h3><div>SPRCA showed strong correlation with CAT (DTT) for IgG titers (r = 0.949; κ = 0.71), with 91.4 % of values within ±1 dilution. Mean IgG TAT was significantly lower for SPRCA (65.26 ± 14.41 min) compared to CAT (DTT) (111.43 ± 19.5 min; p &lt; 0.0001). SPRCA demonstrated minimal inter-technologist variability and elimination of manual pre-DTT treatment steps translating to faster, more consistent results, enabling timely therapeutic decisions.</div></div><div><h3>Conclusion</h3><div>SPRCA is a robust, automated, and IgG-specific platform that overcomes key limitations of CAT. Its implementation in transplant workflows can enhance standardization, reduce delays, and support better outcomes, particularly critical in high-throughput ABOi transplant settings.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104198"},"PeriodicalIF":1.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating recurrent immune-mediated thrombotic thrombocytopenic purpura (iTTP) in pregnancy: A case report and literature review","authors":"Mohadese Golsorkhi ,&nbsp;Niloufar Ebrahimi ,&nbsp;Shahram Kordasti ,&nbsp;Sayna Norouzi ,&nbsp;Amir Abdipour","doi":"10.1016/j.transci.2025.104199","DOIUrl":"10.1016/j.transci.2025.104199","url":null,"abstract":"<div><div>Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a rare and potentially fatal thrombotic microangiopathy characterized by thrombocytopenia and microangiopathic hemolytic anemia. It primarily affects women of childbearing age, with pregnancy being a significant precipitating factor. TTP overlaps with other pregnancy-related conditions, such as HELLP syndrome, complicating diagnosis and necessitating urgent clinical evaluation and intervention. Plasma-based therapies have significantly improved the prognosis for these patients. Additionally, serial follow-up of ADAMTS-13 activity is recommended at the preconception stage and throughout pregnancy for women with a history of TTP. In this report, we present a case of a 32-year-old patient with a recurrent iTTP on her 11-week gestation of the second pregnancy, which was successfully managed with therapeutic plasma exchange (TPE) and corticosteroids.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104199"},"PeriodicalIF":1.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of age and gravidity on CD34+ yields in female peripheral blood stem cell donors","authors":"Hong Lin ,&nbsp;Danning Li ,&nbsp;Jamie Thompson ,&nbsp;David Winstone ,&nbsp;Alan Lankester ,&nbsp;Ying Li","doi":"10.1016/j.transci.2025.104196","DOIUrl":"10.1016/j.transci.2025.104196","url":null,"abstract":"<div><div>Donor age has long been recognized as negatively affecting CD34 + yield following Peripheral Blood Stem Cell (PBSC) mobilization. However, its impact on male versus female donors has not been separately examined. In addition, the potential influence of pregnancy history on female donors remains underexplored. The retrospective study extracted donation data from 267 female donors with known pregnancy history, grouped by age and number of gravidities, and CD34 + yields post-PBSC mobilization were compared. The study revealed a decline in CD34 + yields with increasing donor age, but an elevation in yields among female donors aged 41–50, a pattern not seen with male donors. There was no difference in yields between nulligravida donors and those with 2 or 3 gravidities, and the latter cohort showed significantly higher yields than donors with 1 or 4 + gravidities. For donors in their 40 s, CD34 + yields were either higher than or similar to those of younger donors with the same number of gravidities, suggesting an additional effect of this age group on stem cell yields. Nevertheless, recipients’ engraftment outcomes were comparable among donors with varying numbers of gravidities and nulligravida donors. This study demonstrates that female donors aged 41–50 have CD34 + yields similar to male donors, and those with 2–3 previous pregnancies exhibit CD34 + yields comparable to those of nulligravida donors and significantly higher than those of other gravidity groups. These findings suggest that age and pregnancy history influence stem cell mobilisation, emphasizing the need for further research into their underlying biological mechanisms and clinical significance.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104196"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of storage temperature and time on growth factor concentration in single-harvest platelet-rich plasma","authors":"Yulin Wang ,&nbsp;Xiaona Fu ,&nbsp;Dongdong Cao","doi":"10.1016/j.transci.2025.104197","DOIUrl":"10.1016/j.transci.2025.104197","url":null,"abstract":"<div><h3>Introduction</h3><div>Based on its therapeutic potential, the Platelet-rich plasma (PRP) therapy doubled in interest. Storage of this blood product in appropriate conditions allows for many interventions and increased clinical use. This research was intended to determine how the change in the storage temperature and time affects the concentrations of the essential growth factors of the PRP, which determines its biological activity.</div></div><div><h3>Methods</h3><div>PRP was prepared from 10 healthy donors and stored at room temperature (RT; 22°C), 4°C, and −65°C. Aliquots were analyzed at baseline (0 days), 1, 3, and 6 days post-preparation. Growth factors platelet-derived growth factor-AA (PDGF-AA), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), and fibroblast growth factor-basic (b-FGF) were quantified after activation using Triton-X (PDGF-AA, VEGF, b-FGF) or sonication (TGF-β1), with sandwich immunoassays.</div></div><div><h3>Results</h3><div>PDGF-AA concentrations consistently declined over time across all storage conditions. In contrast, TGF-β1 levels progressively increased, reaching peak concentrations by day 6. VEGF exhibited a reduction in samples stored at RT and −65 °C, while b-FGF concentrations dropped sharply after 24 h in these same groups. Refrigeration (4 °C) showed intermediate effects on growth factor stability compared to other temperatures.</div></div><div><h3>Conclusion</h3><div>Growth factor concentrations in PRP varied significantly depending on the activation technique, with no difference in activators for the same growth factor. These findings underscore the importance of optimizing storage protocols to preserve PRP’s bioactive properties. For clinical efficacy, PRP should be activated and stored under conditions that maintain growth factor integrity, ensuring maximal therapeutic benefit.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104197"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of peripheral stem cell mobilization research progress (2000–2023) based on bibliometric data","authors":"Semih Basci,&nbsp;Sinem Namdaroglu,&nbsp;Hikmetullah Batgi","doi":"10.1016/j.transci.2025.104189","DOIUrl":"10.1016/j.transci.2025.104189","url":null,"abstract":"<div><h3>Background</h3><div>Peripheral stem cell mobilization is a key process in hematopoietic stem cell transplantation (HSCT), enabling efficient stem cell collection from peripheral blood. This bibliometric study provides a comprehensive analysis of research trends, major publications, and emerging themes in this field.</div></div><div><h3>Material and methods</h3><div>In this study, bibliographic research on peripheral stem cell mobilization was carried out on peripheral stem cell mobilization using the Web of Science Core Collection (WoSCC) database. Data was gathered from the Science Citation Index Expanded and Emerging Sources Citation Index databases. Bibliometrics was used to evaluate trends in the scientific literature. The study used the Bibliometrix R package for quantitative sociometric research, enabling seamless data extraction and analysis of scholarly literature interconnectedness and trends.</div></div><div><h3>Results</h3><div>The analysis of 293 publications from 2000 to 2023 shows a declining growth trend. There were 19.63 citations per article. Collaborative efforts involve 2176 authors, with an average of 8.74 co-authors per document and a 10.58 % international co-authorship rate. The United States leads in annual article output, while Europe and Asia contribute prominently. Notable journals include \"Bone Marrow Transplantation\" and \"Transfusion and Apheresis Science.\" Keyword analysis highlights key themes in hematopoietic stem cell transplantation research. Top cited articles and detailed citation impact analysis provide insights into global scientific production, trends, and collaborative dynamics.</div></div><div><h3>Discussion</h3><div>This bibliometric article provides a comprehensive overview of peripheral stem cell mobilization research, offering valuable insights for researchers and clinicians in advancing hematopoietic stem cell transplantation and related therapies.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104189"},"PeriodicalIF":1.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting the development of hypokalemia during apheresis in healthy donors","authors":"Wataru Kitamura ,&nbsp;Keiko Fujii ,&nbsp;Hiroki Kobayashi ,&nbsp;Masaya Abe ,&nbsp;Takuya Fukumi ,&nbsp;Kazuhiro Ikeuchi ,&nbsp;Fumio Otsuka ,&nbsp;Yoshinobu Maeda ,&nbsp;Nobuharu Fujii","doi":"10.1016/j.transci.2025.104195","DOIUrl":"10.1016/j.transci.2025.104195","url":null,"abstract":"<div><div>Despite being generally safe, apheresis for peripheral blood stem cell collection potentially disrupts electrolyte balance owing to the use of citric acid as an anticoagulant. As prior research has primarily studied hypocalcemia, information on the kinetics of potassium levels during apheresis in healthy donors is scarce. We investigated the fluctuation in potassium levels during apheresis and the risk factors for hypokalemia. This subanalysis used data from an open-label, randomized controlled trial of “oral calcium supplementation versus placebo in mitigating citrate toxicity” conducted between January 2021 and July 2022, at Okayama University Hospital. Potassium levels were significantly reduced after 5-day granulocyte colony-stimulating factor (G-CSF) administration (<em>p</em> &lt; 0.0001), with seven patients (16.7 %) given oral potassium administration before apheresis because the treating physician deemed potassium levels potentially unsafe and three (7.1 %) presenting with hypokalemia at apheresis. Potassium levels after apheresis were significantly lower than those before apheresis (baseline; <em>p</em> &lt; 0.0001), and 28 of 42 donors (66.7 %) experienced biochemical, clinically unapparent hypokalemia immediately after the completion of apheresis. A &gt; 15 % reduction in potassium levels from baseline was associated with age and the acid citrate dextrose solution A (ACD-A) volume in univariate analysis. In the multivariable analysis, both factors were associated (hazard ratio [HR], 11.60; 95 % confidence interval [CI], 1.60–83.70; <em>p</em> = 0.02 and HR, 17.50; 95 % CI, 1.07–136.00; <em>p</em> = 0.04). In conclusion, G-CSF administration and apheresis ultimately induced hypokalemia in two-thirds of the donors. Older age and higher ACD-A volume may affect potassium levels during apheresis in healthy donors.</div><div><strong>Clinical Trial registration:</strong> jRCTs061200035.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104195"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mastering quality in transfusion medicine: Establishing best practices through global expert collaboration – September 2025","authors":"Jerard Seghatchian","doi":"10.1016/j.transci.2025.104193","DOIUrl":"10.1016/j.transci.2025.104193","url":null,"abstract":"","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 4","pages":"Article 104193"},"PeriodicalIF":1.4,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}