Transfusion and Apheresis Science最新文献

{"title":"Clinical application of erythrocytapheresis in the treatment of erythrocytosis","authors":"Zhongmei Yi ,&nbsp;Fang Zhou ,&nbsp;Yumeng Zhou ,&nbsp;Chunxi Wu ,&nbsp;Bin Zhang","doi":"10.1016/j.transci.2025.104159","DOIUrl":"10.1016/j.transci.2025.104159","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to clarify the clinical utility of erythrocytapheresis as a treatment for erythrocytosis, including polycythemia vera and secondary erythrocytosis.</div></div><div><h3>Material and methods</h3><div>A retrospective analysis was conducted on 83 patients (84.34 % male, mean age 49.66 years (16.23 years)) who underwent a total of 104 erythrocytapheresis sessions between September 2021 and April 2025. The key parameters measured included the depleted red blood cell (RBC) volume, the hemoglobin (HB) and hematocrit (Hct) reductions, and adverse reactions.</div></div><div><h3>Results</h3><div>Erythrocytapheresis significantly reduced the post-treatment RBC counts (25.22 %), the HB (25.19 %) level and the Hct (23.62 %), the white blood cell counts, (23.37 %) and the platelet counts (8.38 %) (all <em>p</em> &lt; 0.05). The median depleted RBC volume was 1405.5 mL (range 1011.75–1677.5 mL). The median post-treatment target Hct was 40 % (range 40–45 %). The mean post-treatment Hct was in fact 47.57 % (6.54 %), which was higher than the target, and the mean Hct error ratio was 14.23 % (12.95 %). When the replacement solution was physiological saline, an adverse reaction (hypotension) occurred during 4.81 % of the 104 erythrocytapheresis sessions, but only in patients with a depleted RBC volume/weight (D/W) ≥ 25 mL/kg. No significant differences were observed between the Spectra Optia and COM.TEC blood cell separators.</div></div><div><h3>Discussion</h3><div>Erythrocytapheresis effectively reduces the RBC mass in patients with erythrocytosis.In rough terms, the post-treatment target Hct = the post-treatment Hct × 0.88, our research team has officially designated this formula as the 'Southwest Formula'. In the future, we will continue to expand the sample size to enhance the precision of this formula and better guide the clinical application of erythrocytapheresis.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104159"},"PeriodicalIF":1.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of platelet transfusion practices in China: A nationwide survey on transfusionists’ preferences and barriers to whole blood-derived platelets adoption","authors":"Tie Cheng Sun ,&nbsp;Yu Jie Wen ,&nbsp;Ning Zhang ,&nbsp;Di Wu ,&nbsp;Wei Dong Zhang ,&nbsp;Yan Jun Jia","doi":"10.1016/j.transci.2025.104156","DOIUrl":"10.1016/j.transci.2025.104156","url":null,"abstract":"<div><h3>Background</h3><div>Despite the potential of whole blood-derived platelets (WBDP) to mitigate platelet shortages, their underutilization persists globally. This study investigates the perceptions of transfusionists and identifies barriers to the adoption of WBDP within China's blood supply system for tertiary hospitals.</div></div><div><h3>Materials and methods</h3><div>A structured questionnaire comprising 16 items was distributed through WeChat to 254 transfusionists working in tertiary hospitals across China. Data regarding demographics, platelet preferences, and operational challenges were analyzed using descriptive statistics for quantitative data and thematic analysis for qualitative responses.</div></div><div><h3>Results</h3><div>Results indicated a strong preference for AP (95.28 %), primarily attributed to perceived safety (80.99 %), efficacy (75.21 %), and familiarity with its use (76.86 %). In contrast, WBDP adoption was impeded by concerns over transfusion reactions (85.04 %), safety uncertainties (77.20 %), and operational challenges, such as increased workload (74.41 %) and higher labor costs (50.39 %). Despite this, 90.19 % of respondents expressed willingness to use WBDP during AP shortages, primarily due to critical shortages (66.81 %) and institutional mandates (33.19 %). Moreover, the key barriers included the risk of transfusion reactions (85.04 %), uncertainties regarding safety (77.20 %), doubts about efficacy (57.48 %), and increased labor costs (50.39 %).</div></div><div><h3>Conclusion</h3><div>Institutional inertia favoring AP, along with operational costs and workload for transfusionists, limit WBDP utilization. Implementing centralized compatibility testing and subsidy policies could improve contingency preparedness and address operational challenges.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104156"},"PeriodicalIF":1.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The RhCcEe phenotype incompatibility in Chinese and presence of DEL in serologically D–, C/E+ individuals","authors":"Yuqi Xiong,&nbsp;Shuying Zou,&nbsp;Xin Zou,&nbsp;Sifei Ma,&nbsp;Hongmei Yang","doi":"10.1016/j.transci.2025.104157","DOIUrl":"10.1016/j.transci.2025.104157","url":null,"abstract":"<div><h3>Background</h3><div>Red cell antibodies of a certain specificity are produced following the immunization of a corresponding antigen-negative individual through pregnancy or transfusion, such as RhCcEe allogeneic infusion. The transfusion incompatibility of RhC/c and RhE/e can be estimated based on the proportion of the RhCcEe phenotype. DEL has been positively correlated with RhC and RhE phenotypes. Elucidating the RhCcEe phenotype may facilitate the identification and screening of DEL variants.</div></div><div><h3>Objective and methods</h3><div>The risk of alloimmunization due to RhC/c and RhE/e incompatibility were estimated by analyzing the frequency of RhCcEe phenotypes in 783 RhD negative blood donors and more than 458,000 RhD positive individuals. DEL screening was performed on RhC+ and/or RhE+ samples from RhD negative population. A total of 106 RhC+ and(or) RhE+ RhD negative blood donors were selected for DEL screening using an absorption and elution test, fluorescent probe-based quantitative PCR, and <em>RHD</em> sequencing.</div></div><div><h3>Results</h3><div>Significant differences in RhCcEe phenotype distribution were observed between RhD positive and RhD negative Chinese individuals. Among RhD-positive Chinese individuals, the transfusion incompatibility rates for E/e and C/c were notably high at 31.09 % and 34.04 %, respectively. The highest alloimmunization risks were observed for anti-c (24.889 %), followed by anti-E (24.731 %). The RhD negative population had the highest alloimmunization risk of anti-C(24.257 %). Among the 106 RhC+ and(or) RhE+ D-negative samples, thirty-four DELs were observed, with a proportion of 16.27 %. Thirty-two DELs were confirmed to have the <em>RHD 1227G&gt;A</em> allele. Two DEL cases have <em>RHD-CE-D</em> hybrid alleles, including one <em>RHD-CE(3−9)-D</em> and one <em>RHD-CE(3−6)-D.</em></div></div><div><h3>Conclusion</h3><div>We have determined the prevalence of RhCcEe phenotypes in Chinese and derived the incompatibility of E/e and C/c in the same population, enabling the estimation of their potential alloimmunization risk. The RhC+ phenotype, in conjunction with the anti-D adsorption-elution test and the <em>RHD 1227A</em> allele PCR technique, can effectively differentiate true RhD-negative individuals from those with the DEL phenotype.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104157"},"PeriodicalIF":1.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allelic variations in the genes coding for human platelet antigens in four population groups in Borneo","authors":"Mat Ghani Siti Nor Assyuhada ,&nbsp;Che Ghazali Norul Hajar ,&nbsp;Siti-Azhani Amran ,&nbsp;Zefarina Zulkafli ,&nbsp;Nilie Syamila Mohammad Isa ,&nbsp;Nur Saiyidah Hanisah Ali ,&nbsp;Siti Aisyah Mohamad ,&nbsp;Fika Tri Anggraini ,&nbsp;Geoffrey Keith Chambers ,&nbsp;Hisham Atan Edinur","doi":"10.1016/j.transci.2025.104158","DOIUrl":"10.1016/j.transci.2025.104158","url":null,"abstract":"<div><h3>Introduction</h3><div>Human platelet antigens (HPAs) are clinically significant markers in immune-mediated disorders. Comprehensive understanding of HPA genetic profiles at population level is essential for the prevention and management of these medical conditions. Such data for HPA allele frequencies have been collected for many ethnic groups in Peninsular Malaysia, but none from Sabah and Sarawak of Borneo. This study aims to determine the frequencies of HPA alleles in Bajau, Kadazan-Dusun, Murut and Melanau populations of Borneo.</div></div><div><h3>Methodology</h3><div>Seven HPA loci (HPA-1 to −6 and −15) were genotyped using the polymerase chain reaction assay with sequence specific primers for 457 DNA samples representing four Borneo indigenous groups.</div></div><div><h3>Results</h3><div>Significant departures from Hardy-Weinberg equilibrium (HWE) were observed for HPA-3 in Bajau, Kadazan-Dusun, Murut and Melanau groups and for HPA-15 in Bajau and Murut. High frequencies of HPA-1a, −2a, −4a, −5a, and −6a alleles were observed across all four populations. Exact tests showed significant differentiation between Borneo and Peninsular Malaysia populations, particularly for HPA-3 and −15. Principal component (PCA) analysis showed that the Borneo indigenous groups clustered closely with other Austronesian populations, consistent with the idea that they share genetic ancestry.</div></div><div><h3>Conclusion</h3><div>Observed HPA allele frequency spectra are uniquely distributed in Bajau, Kadazan-Dusun, Murut and Melanau population groups. The HPA population datasets generated from this study can be used as guidelines for health policy decision making and as reference sources for studying diseases associated with HPAs.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104158"},"PeriodicalIF":1.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Jerard Seghatchian ,&nbsp;Paulo Pereira","doi":"10.1016/j.transci.2025.104155","DOIUrl":"10.1016/j.transci.2025.104155","url":null,"abstract":"","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104155"},"PeriodicalIF":1.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GMP in donor centres","authors":"Rainer Moog","doi":"10.1016/j.transci.2025.104148","DOIUrl":"10.1016/j.transci.2025.104148","url":null,"abstract":"<div><h3>Background</h3><div>Collection and production of blood components should be in line with current blood guidelines to ensure both donor safety and the quality of the blood product. Guidelines were developed for the whole process of blood collection and processing as well as for apheresis procedures. Implementation of such Good Manufacturing Practice (GMP) is mandatory for donor centres.</div></div><div><h3>Design</h3><div>Principles of GMP and its impact on quality management for the donor centre are reviewed in the present paper. Relevant GMP chapters and practical issues show how to manage GMP.</div></div><div><h3>Results</h3><div>The European GMP guideline helps to standardize the production of medicinal products. Requirements for quality control and management, documentation and manufacturing are described in GMP guidelines. Risk management helps to identify and quantify failures in the production process.</div></div><div><h3>Conclusion</h3><div>Implementation of GMP ensures donor and product safety.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104148"},"PeriodicalIF":1.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Findings in the Immuno-hematology workshop during the International Congress of WAA 2024 in Indonesia","authors":"A.M.E. Sundermeijer ,&nbsp;H. Vrielink","doi":"10.1016/j.transci.2025.104137","DOIUrl":"10.1016/j.transci.2025.104137","url":null,"abstract":"","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104137"},"PeriodicalIF":1.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-world evidence on effectiveness and safety of granulocyte transfusion (GT) during pre-engraftment phase in hematopoietic stem cell transplant patients: A single-centre study from north India, with mini-review of Indian studies on GT","authors":"Pragya Silwal ,&nbsp;Aseem Kumar Tiwari ,&nbsp;Geet Aggarwal ,&nbsp;Shubham Gupta ,&nbsp;S.P. Yadav ,&nbsp;Nitin Sood","doi":"10.1016/j.transci.2025.104127","DOIUrl":"10.1016/j.transci.2025.104127","url":null,"abstract":"<div><h3>Introduction</h3><div>Pre-engraftment phase of hematopoietic stem cell transplant (HSCT) (&lt;3 weeks after transplant) is a crucial period with chances of early complications like febrile neutropenia. Granulocyte transfusion (GT) is a temporary bridge therapy to recover such patients till engraftment.</div></div><div><h3>Methods</h3><div>This was a retrospective observational study conducted by the department of Transfusion Medicine at a large tertiary care hospital in North India from January 2020 to June 2023. The study included all consecutive HSCT patients who received GT(s) during the pre-engraftment phase.</div></div><div><h3>Results</h3><div>Granulocyte was transfused to all post-HSCT patients fulfilling the criteria for GT. Donors were screened as per standard guidelines and apheresis was done for accepted donors. The mean dose of the product was 1.45 × 10¹⁰ and the mean comparison of pre- and post-procedure neutrophil count was statistically significant (&lt;0.05). There was successful 30-day survival in all the patients.</div></div><div><h3>Conclusion</h3><div>Although various studies have been done to prove the efficacy of GT, further research is recommended to formulate standard Indian guidelines.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104127"},"PeriodicalIF":1.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial commentary: Spotlights on the latest trends in innovations in blood transfusion quality assurance practices","authors":"Jerard Seghatchian","doi":"10.1016/j.transci.2025.104151","DOIUrl":"10.1016/j.transci.2025.104151","url":null,"abstract":"","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104151"},"PeriodicalIF":1.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biographies of the guest editors for the theme papers on proceedings of the WAA meeting","authors":"Hans Vrielink ,&nbsp;Teguh Triyono","doi":"10.1016/j.transci.2025.104134","DOIUrl":"10.1016/j.transci.2025.104134","url":null,"abstract":"","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104134"},"PeriodicalIF":1.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}