Transfusion and Apheresis Science最新文献

{"title":"Precision diagnostics in Transfusion Medicine: Advancing accuracy in Bombay blood group typing through molecular methods","authors":"Shamee Shastry ,&nbsp;Akshay Chopra ,&nbsp;Yew-Wah Liew ,&nbsp;Glenda Millard ,&nbsp;Deepika Chenna ,&nbsp;Ganesh Mohan ,&nbsp;Deep Madkaiker","doi":"10.1016/j.transci.2025.104114","DOIUrl":"10.1016/j.transci.2025.104114","url":null,"abstract":"<div><h3>Background</h3><div>Integration of precision diagnosis in Immunohaematology and Transfusion Medicine has led to the development of more refined blood typing and crossmatching techniques, ensuring improved accuracy of blood group determination, thereby reducing the incidence of transfusion reactions and enhancing patient safety. It can also help resolve complex discrepancies noted in serological testing methods.</div></div><div><h3>Study design and methods</h3><div>The blood grouping of a 40 year old female patient done as a part of routine health check using column agglutination technology showed a O group phenotype with additional reaction with O cells in the serum group. Anti-H lectin study confirmed the absence of H antigen, with a Lewis Le(a+b-) phenotype, typing the patient as Classical Bombay phenotype. Further molecular workup was performed.</div></div><div><h3>Results</h3><div>An <em>in-house</em> designed panel enabling comprehensive genotyping for 45 blood group systems and transcription factors <em>KLF1</em> and <em>GATA1</em> was used<em>.</em> Sequencing covering the ABO gene predicted the presence of the <em>ABO*O.01.01</em> and <em>ABO*O.01.02</em> alleles and the group O phenotype. Combined results of the sequencing analysis identified <em>ABO*O.01.01/*O.01.02</em>, <em>FUT1*01N.09/*01N.09</em> and <em>FUT2*01/*01</em> with the predicted phenotype of H-deficient; secretor (para-Bombay). Oh-secretor shows the patient is genetically a ParaBombay since there is an active secretor gene, but phenotyped as Le(a+b-) due to the reduction in Lewis enzyme function by the FUT3 mutations. Hence the patient is classified as a paraBombay, even though the Lewis phenotype make the patient appeared to be a classical Bombay.</div></div><div><h3>Conclusion</h3><div>Integration of precision diagnostics into transfusion therapy improves transplant and transfusion safety.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104114"},"PeriodicalIF":1.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of transfusion transmitted infections by mode of donation and remuneration status among blood donors in Georgia, 2018–2023","authors":"Ketevan Shermadini ,&nbsp;Evan M. Bloch ,&nbsp;Shaun Shadaker ,&nbsp;Maia Alkhazashvili ,&nbsp;Nazibrola Chitadze ,&nbsp;Sophia Surguladze ,&nbsp;Irinka Tskhomelidze ,&nbsp;Vladimer Getia ,&nbsp;Ekaterine Adamia ,&nbsp;Rania A. Tohme ,&nbsp;Tamar Gabunia","doi":"10.1016/j.transci.2025.104110","DOIUrl":"10.1016/j.transci.2025.104110","url":null,"abstract":"<div><div>Global reform of blood transfusion services is underway in the country of Georgia. New legislation mandates exclusive collection of blood from non-remunerated blood donors in Georgia by July 2025. Retrospective data (2018–2023) from the National Blood Donor Registry were analyzed. The prevalence was calculated for human immunodeficiency virus (HIV) antigen/antibody (Ag/Ab), hepatitis C virus antibody (anti-HCV), hepatitis B surface antigen (HBsAg). Results were stratified by remuneration status and mode of donation. Descriptive analysis was performed to elucidate differences in positivity by year and donor type. During 2018–2023, there were a total of 548,530 donations from 221,492 blood donors in Georgia; 68.3 % of donors were male and the median age was 34 years (interquartile range: 26<img>44). Overall, 17.0 % were paid, 24.9 % were replacement, 47.4 % were voluntary non-remunerated blood donors (VNRBD), and 10.7 % had ≥ 2 donations of varying remuneration type. Paid donors had an average of 2.5 donations per year, compared to 1.0 for replacement, and 1.1 for VNRBDs<em><strong>.</strong></em> During 2018–2023, the proportions of paid donors decreased (38.8–22.1 %); the proportions of replacement (19.1–26.0 %) and VNRBDs (38.7–48.3 %) increased. Among first-time donors, prevalence decreased during 2018–2023 for anti-HCV (2.0–0.9 %) but were stable for HBsAg (range: 1.9 %-2.1 %) and anti-HIV (range: 0.1 %-0.2 %). Among repeat donors, prevalence of anti-HCV decreased (from 0.3 % to 0.2 %) while rates were stable for anti-HIV (0.04 %-0.1 %), and HBsAg (0.1 % in all years). The findings underscore the importance of donor retention in concert with efforts to attain exclusive VNRBD.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104110"},"PeriodicalIF":1.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"eHealth technologies for enhancing blood donor knowledge and behaviour: A PRISMA scoping review","authors":"Emmanuel Nene Dei ,&nbsp;Sharon Leitch","doi":"10.1016/j.transci.2025.104112","DOIUrl":"10.1016/j.transci.2025.104112","url":null,"abstract":"<div><h3>Background</h3><div>Blood donor information and education are pivotal in improving knowledge and encouraging positive behaviours towards blood donation. Their impact of utilizing eHealth technologies on self-efficacy presents new opportunities to improve knowledge and encourage people to donate blood.</div></div><div><h3>Objective</h3><div>A scoping review was conducted to understand the nature of the scientific evidence on improving knowledge and influencing the behaviours of blood donors with eHealth technologies.</div></div><div><h3>Methods</h3><div>Through a systematic literature search, 6261 published papers were screened, and 20 were reviewed.</div></div><div><h3>Results</h3><div>eHealth technologies commonly utilized were audio/telephone calls, text messages, emails, audio-visuals, mobile health and smartphone applications, custommade software applications and social media. They were mainly used to disseminate educational and motivational information, send reminders, and enhance the blood donation experience. They significantly impacted the knowledge and behaviours of blood donors, including increased blood donation rates and repeat blood donations, and enhanced blood donor awareness, motivation and behaviours. Facilitators for their effective utilization included personalisation, organisational support, user-centred design and interactivity, clarity and privacy, automation, and cultural acceptance. Barriers included technical, communication, usability, poor access, low awareness and operational limitations.</div></div><div><h3>Conclusions</h3><div>eHealth technologies could be integrated into traditional blood donor programs even though they are not a panacea for all blood donor recruitment challenges. Targeted research is needed to understand their long-term effectiveness and applicability. Future research should address gaps identified, particularly focusing on the impact of contextual differences and health literacy. Such research will promote the development of more effective and inclusive blood donor engagement programs and technologies.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104112"},"PeriodicalIF":1.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A serological and molecular study on a case of Rh blood group mosaicism","authors":"Mingwei Yin ,&nbsp;Xinyu Huang ,&nbsp;Jing Pian ,&nbsp;Jihua Ma ,&nbsp;Xiaozhen Hong ,&nbsp;Xuejun Chen ,&nbsp;Xianguo Xu","doi":"10.1016/j.transci.2025.104111","DOIUrl":"10.1016/j.transci.2025.104111","url":null,"abstract":"<div><h3>Background</h3><div>Mosaicism refers to the presence of two or more genetically different cell lines within a single individual, the majority of which are accidentally discovered through routine blood group identification. Here, we report one case of Rh blood group mosaicism in a 10-year-old Chinese male.</div></div><div><h3>Methods</h3><div>Serological tests for the Rh blood group were performed via gel microcolumn tests and saline methods. Mosaic red blood cells (RBCs) were quantified via flow cytometry. The G-banded karyotype was analyzed at a 550-band resolution. Short tandem repeat (STR) analysis was used to compare the allele repeats at specific loci in DNA between blood, buccal mucosal, and hair shaft samples.</div></div><div><h3>Results</h3><div>Mixed-field agglutination with anti-C and anti-e antibodies was identified in the gel microcolumn test. The proportion of those positive for the C antigen was 47.26 %, while that of the negative ones was 52.74 %, as calculated by flow cytometry, and the double populations were identified as DCcEe- and DccEE-phenotype RBCs by multiple centrifugations. Karyotype analysis revealed 46, XY in the proband. STR analysis revealed that additional paternal alleles were present at the D1S1656, CSF1PO, and Penta D loci.</div></div><div><h3>Conclusion</h3><div>An individual with natural Rh blood group mosaicism was identified.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104111"},"PeriodicalIF":1.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden in plain sight: Detecting rare naturally occurring anti-K antibody in a blood donor","authors":"Rutuja Baldota ,&nbsp;Brinda Kakkar ,&nbsp;Sanjiv Ketkar ,&nbsp;Manaswi Patil","doi":"10.1016/j.transci.2025.104109","DOIUrl":"10.1016/j.transci.2025.104109","url":null,"abstract":"<div><div>Red cell alloimmunisation is commonly reported among pregnant, transplant and multitransfused patients, whereas it is a fairly rare occurrence in the healthy general population. We report a rare case where we detected naturally occurring anti-K antibody in a healthy male whole blood donor during routine antibody screening.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104109"},"PeriodicalIF":1.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence and pattern of alloimmunization in patients with sickle cell disease in Abuja, Nigeria","authors":"Okeke Chinedu ,&nbsp;Isa Alkali Hezekiah ,&nbsp;Oyetunde Bankole Akinloye ,&nbsp;Asala Samuel ,&nbsp;Akinyemi Olugbenga Ofakunrin ,&nbsp;Oche Patrick Ogbe ,&nbsp;Rose Amaechi ,&nbsp;Obiageli Eunice Nnodu","doi":"10.1016/j.transci.2025.104107","DOIUrl":"10.1016/j.transci.2025.104107","url":null,"abstract":"<div><h3>Background</h3><div>One of the components of comprehensive care and interventions in managing patients with sickle cell disease (SCD) is red blood cell transfusion. This has helped in reducing the morbidity and mortality associated with SCD. Individuals who undergo multiple red cell transfusions are faced with alloimmunization which makes it difficult to source compatible blood for them. This often leads to haemolytic reactions and sometimes death of the patients. In this study, we determined the prevalence and pattern of alloimmunization in patients with SCD.</div></div><div><h3>Methods</h3><div>This was a cross-sectional study of 205 patients with SCD aged 1–45 years, attending the sickle cell clinic at the University of Abuja Teaching Hospital, Gwagwalada, Abuja. Antibody detection and identification were done using the tube technique (Lone Laboratories UK). The data was analyzed and reported in percentages and descriptive statistics.</div></div><div><h3>Results</h3><div>Of the 205 participants, 115 (56.1 %) were males while 90 (43.9 %) were females. 99 (48.8 %) of the patients had been transfused two or more times while 49(23.9 %) were transfused only once. Fifty-seven (27.8 %) participants have never had a transfusion. The following blood group systems were observed in 26 alloimmunized SCD participants; Rh (D/E/e/C/Cw) 46 %, Kell19.2 %, Lewis 11.5 %, MNS 3.8 %, Duffy 23.0 %. All 26 participants have had more than one episode of blood transfusion.</div></div><div><h3>Conclusion</h3><div>The prevalence rate was 17.6 % and Rh alloantibodies were common among patients with SCD who have received multiple transfusions. Proper blood detection and identification of alloantibodies should be promoted before blood transfusion to manage these patients better.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104107"},"PeriodicalIF":1.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel allele of B(A) blood group detected in a donor and a patient during a retrospective review of ABO group anomalies in a tertiary hospital","authors":"Kanyapon Suksard ,&nbsp;Glenda M. Millard ,&nbsp;Usana Teravichitchainan ,&nbsp;Parichart Permpikul ,&nbsp;Janejira Kittivorapart","doi":"10.1016/j.transci.2025.104108","DOIUrl":"10.1016/j.transci.2025.104108","url":null,"abstract":"<div><h3>Background</h3><div>ABO discrepancies, inconclusive results between forward and reverse typing, are one of the significant challenges encountered in transfusion medicine. Their frequency and etiologies can vary among ethnicities. This study aimed to characterize ABO discrepancies in a Thai population.</div></div><div><h3>Methods</h3><div>We conducted a retrospective review of 285,450 donor and 258,780 patient samples for ABO discrepancies, which were categorized into five groups, as described below. The serological ABO grouping was performed using an automated system, and further serological techniques were used in the discrepancy cases. Additionally, sequencing was used to examine the genetic background of the B(A) phenotype detected during the retrospective review.</div></div><div><h3>Results</h3><div>ABO discrepancies were identified in 396 patients (0.15 %) and 74 blood donors (0.03 %). Among the patients, the most frequent cause was ABO mismatch stem cell transplantation (198; 50 %). The remaining 198 discrepancy cases (198/258,780; 0.08 %) were categorized into five groups: weak/missing red cell reactivity, extra red cell reactivity, mixed-field, weak/missing serum reactivity, and extra serum reactivity, accounting for 17.17 %, 0.51 %, 29.29 %, 28.79 %, and 24.24 %, respectively. For the blood donors, the percentages were 48.65 %, 2.70 %, 2.70 %, 37.84 %, and 8.11 %, respectively. We also identified the B(A) phenotype in one patient and two blood donors. The sequencing study identified allele variants of c.467 C&gt;T, c.796 C&gt;A, c.803 G&gt;C, and c.930 G&gt;A in exon 7, which was a novel allele.</div></div><div><h3>Conclusion</h3><div>ABO discrepancies were distinct between donors and patients even in the same ethnicity. This finding highlighted the influence of the patient’s conditions and therapy on the anomalous ABO typing. Additionally, the B(A) individuals identified in this study carried identical genetic alterations that differed from all antecedent alleles of the B(A) phenotype.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104108"},"PeriodicalIF":1.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfusion-associated circulatory overload: A retrospective audit of risk assessment practices in a large teaching hospital","authors":"Samuel Anokye Badu","doi":"10.1016/j.transci.2025.104105","DOIUrl":"10.1016/j.transci.2025.104105","url":null,"abstract":"<div><h3>Introduction</h3><div>Transfusion-associated circulatory overload (TACO) is a serious transfusion reaction and a leading cause of transfusion-related mortality. It is recommended that all patients requiring blood transfusion should be assessed for the risk of TACO. The 2022 Serious Hazards of Transfusion (SHOT) report indicated a TACO risk assessment compliance rate of 35.6 % across the United Kingdom. This retrospective audit evaluated the TACO risk assessment compliance of a large teaching hospital in Northwest England.</div></div><div><h3>Methodology</h3><div>TACO risk assessments were reviewed from November 2022 to December 2023. Data was collected from paper and electronic patient records. The accuracy of the completed risk assessments was assessed against the 2022 SHOT guidance and patient medical records.</div></div><div><h3>Results</h3><div>44 transfusion pathways were reviewed. 59 % (26/44) had a TACO risk assessment completed, and 41 % (18/44) lacked any risk assessment. 12 % (3/26) ofthe completed risk assessments were incorrect. 50 % (9/18) of the pathways without a risk assessment were at risk of TACO. Despite the identified inaccuracies, no TACO cases were recorded. 43 % (19/26) of completed risk assessments were performed in outpatient, compared to 16 % (7/26) completed in inpatient areas; this may be</div><div>attributable to varying workloads in these clinical areas.</div></div><div><h3>Conclusion</h3><div>The proportion of patients without a TACO risk assessment pretransfusion was significant. A small percentage of the completed assessments were inaccurate, due to missed patient risks or a lack of mitigation plan. Healthcare professionals require improved training to ensure robust peri-transfusion documentation and patient safety.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104105"},"PeriodicalIF":1.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etoposide + cytarabine + pegfilgrastim versus cyclophosphamide + G-CSF for stem cell mobilization in patients with poorly mobilized multiple myeloma and lymphoma","authors":"Yixuan Cheng ,&nbsp;Sishi Xu ,&nbsp;Renzhi Pei ,&nbsp;Dong Chen ,&nbsp;Xiaohong Du ,&nbsp;Shuangyue Li ,&nbsp;Xianxu Zhuang ,&nbsp;Haihui Zhuang ,&nbsp;Ying Fang ,&nbsp;Mengjie Wu ,&nbsp;Peipei Ye ,&nbsp;Ying Lu","doi":"10.1016/j.transci.2025.104096","DOIUrl":"10.1016/j.transci.2025.104096","url":null,"abstract":"<div><h3>Background</h3><div>In multiple myeloma (MM) or lymphoma with poor mobilization, the combination of etoposide, cytarabine (Ara-C), and pegfilgrastim (EAP) appears to have higher mobilization efficacy than cyclophosphamide (Cy) plus granulocyte colony-stimulating factor (G-CSF) (CG) regimens. The purpose of this study was to examine whether there were differences in efficacy and toxicity between the two mobilization regimens.</div></div><div><h3>Methods</h3><div>All data were collected from Department of Hematology at the Affiliated People's Hospital of Ningbo University from May 2016 to March 2023. And data from EAP regimen were compared with data from the CG regimen.</div></div><div><h3>Results</h3><div>A total of 43 patients were mobilized by the EAP regimen and 44 patients by the CG regimen. The target yield of 2 × 10⁶ CD34⁺ cells/kg was achieved in 95.3 % (EAP) and 63.6 % (CG) of patients by 1.3 and 1.6 apheresis (means), respectively. In addition, 76.7 % of patients with EAP regimen and 29.5 % of patients with CG regimen achieved optimal mobilization (≥5 ×10⁶ CD34⁺ cells/kg) during the first apheresis session. The median number of cumulative collected CD34⁺ cells was 8.9 (range 2.6–41.3) × 10⁶ CD34⁺ cells/kg and 4.2 (range 0.1–18.8) × 10⁶ CD34⁺ cells/kg in the two groups, respectively. Thrombocytopenia gr. 4 was observed in 34.9 % of patients after EAP (CG, 2.3 %) and neutropenia gr. 4 was observed in 58.1 % of patients after EAP (CG, 63.6 %).</div></div><div><h3>Discussion</h3><div>EAP is an excellent mobilization regimen with acceptable toxicity and could be considered in patients with MM and lymphoma with poor mobilization.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104096"},"PeriodicalIF":1.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 2024 international survey of platelet products and practice","authors":"Hitoshi Ohto ,&nbsp;Willy A. Flegel ,&nbsp;Pieter F. van der Meer ,&nbsp;Alain M. Ngoma ,&nbsp;Kenneth E. Nollet ,&nbsp;Nabajyoti Choudhury ,&nbsp;Tobias J. Legler","doi":"10.1016/j.transci.2025.104086","DOIUrl":"10.1016/j.transci.2025.104086","url":null,"abstract":"<div><h3>Background</h3><div>Previously, two international surveys have addressed the wider application of platelet collection by apheresis technology and practical issues of platelet transfusion.</div></div><div><h3>Study design and methods</h3><div>A questionnaire was sent to persons with leadership roles related to blood banking and transfusion medicine in their countries/areas/centers, to document the implementation of modern technologies for platelet manufacturing, preservation, and transfusion risk reduction.</div></div><div><h3>Results</h3><div>Responses to the questionnaire finally came from 52 contributors in 40 countries/areas. Adult platelet doses ranged between 2.0×10¹¹ and 4.0×10¹¹ (median 2.5×10¹¹). In approximately 10 % of centers, apheresis platelets comprised more than 90 % of the platelet inventory. More than 70 % of centers adopted universal or near universal leukocyte-reduction by filtration, apheresis, or both. Almost 20 % of centers irradiated all platelet products. Cold-stored platelets were rarely reported; only 3 centers produce such components for 1 % to 5 % of their supply. The use of platelet additive solution was reported by 18 responders (45 %), mainly in Europe, USA, and Australasia. Bacterial detection systems were reported by 18 responders from around the globe. One fatality was reported after transfusion of a platelet product contaminated with <em>Bacillus cereus</em>, whereas no sequelae were observed after transfusion of &gt;350 platelet products contaminated by <em>Cutibacteriae</em>. Pathogen-reduction/pathogen-inactivation technology has been adopted in 15 centers, with little or no extended expiration period. Export of platelets across national borders was extremely rare.</div></div><div><h3>Conclusion</h3><div>With this addition to the literature on platelet transfusion, considerable heterogeneity in collection, processing, and transfusion can be seen across the globe, through which readers may adopt, and adapt, best practices for their unique local circumstances.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 2","pages":"Article 104086"},"PeriodicalIF":1.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}