Transfusion and Apheresis Science最新文献

{"title":"Evaluating strategic blood donor recruitment initiatives: Insights from King Fahd armed forces hospital experience","authors":"Waleed M. Bawazir ,&nbsp;Osama A. Alzahrani ,&nbsp;Ahmed G. Bukhari ,&nbsp;Raed I. Felimban ,&nbsp;Ahmed M. Alaqari ,&nbsp;Ahmed Y. Jubran ,&nbsp;Abdullah A. Almalki ,&nbsp;Wael A. Khallaf ,&nbsp;Ryan M. Alshehri ,&nbsp;Junaid H. Faqih ,&nbsp;Hadeel Al Sadoun ,&nbsp;Wajnat A. Tounsi ,&nbsp;Nora Y. Hakami ,&nbsp;Malik A. Altayar ,&nbsp;Mohammed M. Jalal","doi":"10.1016/j.transci.2025.104119","DOIUrl":"10.1016/j.transci.2025.104119","url":null,"abstract":"<div><h3>Background &amp; aims</h3><div>Ensuring a safe and sufficient blood supply requires effective recruitment strategies, particularly in post-pandemic contexts. This study examines the transition at King Fahd Armed Forces Hospital (KFAFH) from a predominantly patient-related donor base to a focus on volunteer-based mobile and app-facilitated donations. It evaluates key factors influencing this shift and explores the potential for applying this model nationally.</div></div><div><h3>Methods</h3><div>A quasi-experimental retrospective analysis was conducted using KFAFH donation records from 2014 to 2024. Donors were categorized by recruitment type (patient-related, military, mobile, app-based, staff, and other) and compared based on age, gender, first-time vs. repeat status, donation frequency, and infectious disease testing (IDT) outcomes. Donor eligibility criteria and donation intervals were also analyzed. A SWOT analysis evaluated the operational aspects of mobile campaigns, including recruitment strategies, collection sites, and incentive usage.</div></div><div><h3>Results</h3><div>From 2021–2024, mobile and app-based campaigns significantly increased voluntary donations, while patient-related donations decreased by over 50 %. Mobile donors were predominantly repeat donors aged 25–40, with a higher male participation rate. IDT reactivity rates were lower in mobile donors compared to patient-related donors. Mobile drives—held at businesses, schools, and community centers—were supported by targeted outreach, recurring events, and non-monetary incentives. The program demonstrated operational strengths but encountered logistical and resource challenges.</div></div><div><h3>Conclusions</h3><div>Mobile and technology-driven recruitment significantly increased voluntary donations and reduced dependence on patient-related donations at KFAFH. This model shows promise as a replicable strategy for building a more resilient and sustainable national blood supply system.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104119"},"PeriodicalIF":1.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refractory delayed-onset heparin induced thrombocytopenia (HIT) without thrombosis, treated with intravenous immunoglobulin","authors":"Gordana Tomac ,&nbsp;Ivona Horvat ,&nbsp;Jakša Babel ,&nbsp;Koraljka Gojčeta ,&nbsp;Milica Liker ,&nbsp;Ana Boban ,&nbsp;Ines Bojanić","doi":"10.1016/j.transci.2025.104118","DOIUrl":"10.1016/j.transci.2025.104118","url":null,"abstract":"<div><div>Heparin-induced thrombocytopenia (HIT) is a platelet activation syndrome characterized by the sudden onset of thrombocytopenia and/or thrombosis, induced by anti-platelet factor 4 (PF4)/heparin antibodies that cause platelet activation and destruction. Delayed-onset heparin-induced thrombocytopenia is a variant of HIT that occurs in less than 5 % of cases and is characterized by the onset of thrombocytopenia and/or thrombosis several days after heparin discontinuation. With treatment, the platelet count usually recovers within a week, but in up to 1 % of patients, thrombocytopenia persists for several weeks or months, which is referred to as refractory (persisting) HIT. Refractory delayed-onset HIT represents an important clinical entity because of its potential complications as well as the challenges and complexities in diagnostics and treatment. In the following case report, we present a case of a 69-year-old man diagnosed with refractory delayed-onset HIT, confirmed by a functional assay for platelet-activating anti-PF4/heparin antibodies, who prolonged platelet count recovery without evident signs of thrombosis and was successfully treated with intravenous immunoglobulin (IVIG). This case report provides important clinical and laboratory data necessary for the timely recognition, diagnosis, and treatment of refractory delayed-onset HIT. It also emphasizes the need to consider HIT variants and highlights the importance of early recognition and treatment to minimize complications such as thrombosis and bleeding.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104118"},"PeriodicalIF":1.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T cell subgroup analysis and T cell exhaustion after autologous stem cell transplantation in lymphoma patients","authors":"Kayra Somay ,&nbsp;Özgür Albayrak ,&nbsp;Ali Burak Kızılırmak ,&nbsp;Tuba Akan ,&nbsp;Ümit Barbaros Üre ,&nbsp;Olga Meltem Akay ,&nbsp;Burhan Ferhanoğlu ,&nbsp;Elif Birtaş Ateşoğlu","doi":"10.1016/j.transci.2025.104117","DOIUrl":"10.1016/j.transci.2025.104117","url":null,"abstract":"<div><h3>Background</h3><div>Autologous stem cell transplantation (ASCT) is a common treatment option for relapsed/refractory (R/R) lymphomas and it is considered standard of care as primary consolidation therapy for some types of Non-Hodgkin Lymphomas (NHL). Although ASCT benefits patients by allowing cytoreduction with intensive chemotherapy and reconstituting with stem cells, the effects of immunological changes in T cell subgroups after ASCT are still poorly understood.</div></div><div><h3>Objectives</h3><div>We evaluated changes in frequencies of T cell subsets and T cells expressing some of the exhaustion markers (such as LAG-3 and PD-1) from peripheral blood samples before and after ASCT to investigate bone marrow reconstruction and whether exhaustion predicts relapse.</div></div><div><h3>Study design</h3><div>Blood samples were collected on the day before conditioning and at the 1st, 3rd, and 6th months post-ASCT. Flow cytometry analysis was conducted to examine T cell subgroup composition and exhaustion markers, including PD-1 and LAG-3. Additionally, functional analysis was performed using assays for IFN-g and TNF-a production. Furthermore, a CSFE proliferation assay was utilized to assess proliferation capacity.</div></div><div><h3>Results</h3><div>In our data set, dominant cells post-transplantation were memory cells, as the naïve cell population did not recover for 6 months. Both single and combined expressions of LAG-3 and PD-1 were found to be high before transplantation, and decreased after transplantation. However, LAG-3 and PD-1 expression increased in the 3rd and 6th month after transplantation respectively. These changes were more evident for the relapsed patients when compared to non-relapsed patients within 3 months follow-up time. Notably, the expression of inhibitory receptors in the relapsed patients was significantly higher at the first month post-transplantation. CD107a⁺ cytotoxic T lymphocytes (CTL), IFN-g⁺, TNF-a^.+ CTL and T helper lymphocyte (THL) populations significantly decreased in relapsed patients 3rd month after transplantation. Decreased proliferation capacities of CTLs and THLs were also observed in these patients.</div></div><div><h3>Conclusion</h3><div>These results suggest that increased surface PD-1 and LAG-3 expressions along with functional decline after 3 months of ASCT can be used as prognostic data about the relapse status of transplant patients.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104117"},"PeriodicalIF":1.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weak D testing might be discontinued for RhD-negative blood donors with C and E negative phenotypes in the Indian population","authors":"Ayesha Sinha ,&nbsp;Sabita Basu ,&nbsp;Debapriya Basu ,&nbsp;Mahua Reddy ,&nbsp;Mercy Rophina ,&nbsp;Vinod Scaria ,&nbsp;Suvro Sankha Datta","doi":"10.1016/j.transci.2025.104116","DOIUrl":"10.1016/j.transci.2025.104116","url":null,"abstract":"<div><h3>Background</h3><div>Although serological testing to determine weak D status using the antihuman globulin reagent has been phased out for RhD-negative donors in many countries after the availability of <em>RHD</em> genotyping, it is routinely performed in India. However, weak D testing is a resource-intensive and time-consuming process. We devised a new algorithm for weak D testing in RhD-negative blood donors by performing CcEe phenotyping followed by weak D testing in only C+ and/or E + samples and compared it with the existing protocol in terms of time and cost-effectiveness.</div></div><div><h3>Method</h3><div>In this observational study, we tested 500 samples of RhD-negative blood donors over one year in India. The existing and new algorithms were compared, and the agreement was calculated in terms of the test results, time required for testing, and total cost involved. The RhD type, weak D status, and CcEe phenotypes were determined using the conventional tube technique. An adsorption-elution test was performed to check for the Del phenotype. We conducted <em>RHD</em> genotyping for all samples negative for weak D testing.</div></div><div><h3>Results</h3><div>The proposed algorithm showed perfect agreement with the existing protocol (agreement = 100 %; κ= 1.00). By applying the new algorithm, we could reduce 71.63 % of testing time and 24 % of total cost without missing any weak D positive samples in blood donors.</div></div><div><h3>Conclusions</h3><div>Weak D testing might be discontinued for RhD-negative blood donors with C and E negative phenotypes in the Indian population and could be restricted to only C+ and/or E + phenotypes, which seems to be a time- and cost-effective testing strategy.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104116"},"PeriodicalIF":1.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty years of the four-factor prothrombin complex concentrate Octaplex/Balfaxar: A narrative review","authors":"Keyvan Karkouti ,&nbsp;Michelle Gareis ,&nbsp;Chenxi Li ,&nbsp;Hubert Brandstätter ,&nbsp;Alexander Pichotta ,&nbsp;Trupti Mehta Shah ,&nbsp;Joshua N. Goldstein","doi":"10.1016/j.transci.2025.104115","DOIUrl":"10.1016/j.transci.2025.104115","url":null,"abstract":"<div><div>Prothrombin complex concentrate (PCC) is used to boost thrombin potential, support clot formation, and aid in the treatment and prophylaxis of bleeding. The two main forms of PCC are three-factor (3 F-PCC; comprising coagulation factors II, IX, and X) and four-factor (4F-PCC; factors II, VII, IX, X), which contain 25 times the clotting factors found in human plasma. This narrative review summarizes published efficacy and safety data on one 4F-PCC (Octaplex/Balfaxar, Octapharma) within its recognized uses and explores potential applications across different clinical contexts. Clinically available for &gt; 20 years, Octaplex/Balfaxar is supplied as a freeze-dried powder for reconstitution and intravenous infusion. This 4F-PCC contains non-activated forms of coagulation factors as well as anticoagulant proteins C and S, potentially affording a balanced hemostatic effect and mitigating thrombosis risk. Production involves two virus inactivation/removal steps: solvent/detergent treatment and nanofiltration. 4F-PCC is approved for acquired deficiency of vitamin K-dependent clotting factors, such as those induced by vitamin K antagonists (VKAs, e.g., warfarin), and for congenital deficiency of factors II and X. Five published trials in 444 adult patients demonstrated the efficacy of 4F-PCC in VKA reversal, reducing the international normalized ratio (INR) with only two potentially treatment-related thrombotic events reported. While 4F-PCC dosing is currently indicated to be INR-guided, emerging evidence supports fixed dosing as an alternative to conventional weight-based dosing for VKA reversal. Recent guidelines support 4 F-PCC use for direct oral anticoagulant-associated bleeding, cardiac surgery and trauma/emergencies. Ongoing studies will further clarify the efficacy and safety of 4 F-PCC beyond its approved indications.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104115"},"PeriodicalIF":1.4,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision diagnostics in Transfusion Medicine: Advancing accuracy in Bombay blood group typing through molecular methods","authors":"Shamee Shastry ,&nbsp;Akshay Chopra ,&nbsp;Yew-Wah Liew ,&nbsp;Glenda Millard ,&nbsp;Deepika Chenna ,&nbsp;Ganesh Mohan ,&nbsp;Deep Madkaiker","doi":"10.1016/j.transci.2025.104114","DOIUrl":"10.1016/j.transci.2025.104114","url":null,"abstract":"<div><h3>Background</h3><div>Integration of precision diagnosis in Immunohaematology and Transfusion Medicine has led to the development of more refined blood typing and crossmatching techniques, ensuring improved accuracy of blood group determination, thereby reducing the incidence of transfusion reactions and enhancing patient safety. It can also help resolve complex discrepancies noted in serological testing methods.</div></div><div><h3>Study design and methods</h3><div>The blood grouping of a 40 year old female patient done as a part of routine health check using column agglutination technology showed a O group phenotype with additional reaction with O cells in the serum group. Anti-H lectin study confirmed the absence of H antigen, with a Lewis Le(a+b-) phenotype, typing the patient as Classical Bombay phenotype. Further molecular workup was performed.</div></div><div><h3>Results</h3><div>An <em>in-house</em> designed panel enabling comprehensive genotyping for 45 blood group systems and transcription factors <em>KLF1</em> and <em>GATA1</em> was used<em>.</em> Sequencing covering the ABO gene predicted the presence of the <em>ABO*O.01.01</em> and <em>ABO*O.01.02</em> alleles and the group O phenotype. Combined results of the sequencing analysis identified <em>ABO*O.01.01/*O.01.02</em>, <em>FUT1*01N.09/*01N.09</em> and <em>FUT2*01/*01</em> with the predicted phenotype of H-deficient; secretor (para-Bombay). Oh-secretor shows the patient is genetically a ParaBombay since there is an active secretor gene, but phenotyped as Le(a+b-) due to the reduction in Lewis enzyme function by the FUT3 mutations. Hence the patient is classified as a paraBombay, even though the Lewis phenotype make the patient appeared to be a classical Bombay.</div></div><div><h3>Conclusion</h3><div>Integration of precision diagnostics into transfusion therapy improves transplant and transfusion safety.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104114"},"PeriodicalIF":1.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of transfusion transmitted infections by mode of donation and remuneration status among blood donors in Georgia, 2018–2023","authors":"Ketevan Shermadini ,&nbsp;Evan M. Bloch ,&nbsp;Shaun Shadaker ,&nbsp;Maia Alkhazashvili ,&nbsp;Nazibrola Chitadze ,&nbsp;Sophia Surguladze ,&nbsp;Irinka Tskhomelidze ,&nbsp;Vladimer Getia ,&nbsp;Ekaterine Adamia ,&nbsp;Rania A. Tohme ,&nbsp;Tamar Gabunia","doi":"10.1016/j.transci.2025.104110","DOIUrl":"10.1016/j.transci.2025.104110","url":null,"abstract":"<div><div>Global reform of blood transfusion services is underway in the country of Georgia. New legislation mandates exclusive collection of blood from non-remunerated blood donors in Georgia by July 2025. Retrospective data (2018–2023) from the National Blood Donor Registry were analyzed. The prevalence was calculated for human immunodeficiency virus (HIV) antigen/antibody (Ag/Ab), hepatitis C virus antibody (anti-HCV), hepatitis B surface antigen (HBsAg). Results were stratified by remuneration status and mode of donation. Descriptive analysis was performed to elucidate differences in positivity by year and donor type. During 2018–2023, there were a total of 548,530 donations from 221,492 blood donors in Georgia; 68.3 % of donors were male and the median age was 34 years (interquartile range: 26<img>44). Overall, 17.0 % were paid, 24.9 % were replacement, 47.4 % were voluntary non-remunerated blood donors (VNRBD), and 10.7 % had ≥ 2 donations of varying remuneration type. Paid donors had an average of 2.5 donations per year, compared to 1.0 for replacement, and 1.1 for VNRBDs<em><strong>.</strong></em> During 2018–2023, the proportions of paid donors decreased (38.8–22.1 %); the proportions of replacement (19.1–26.0 %) and VNRBDs (38.7–48.3 %) increased. Among first-time donors, prevalence decreased during 2018–2023 for anti-HCV (2.0–0.9 %) but were stable for HBsAg (range: 1.9 %-2.1 %) and anti-HIV (range: 0.1 %-0.2 %). Among repeat donors, prevalence of anti-HCV decreased (from 0.3 % to 0.2 %) while rates were stable for anti-HIV (0.04 %-0.1 %), and HBsAg (0.1 % in all years). The findings underscore the importance of donor retention in concert with efforts to attain exclusive VNRBD.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104110"},"PeriodicalIF":1.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"eHealth technologies for enhancing blood donor knowledge and behaviour: A PRISMA scoping review","authors":"Emmanuel Nene Dei ,&nbsp;Sharon Leitch","doi":"10.1016/j.transci.2025.104112","DOIUrl":"10.1016/j.transci.2025.104112","url":null,"abstract":"<div><h3>Background</h3><div>Blood donor information and education are pivotal in improving knowledge and encouraging positive behaviours towards blood donation. Their impact of utilizing eHealth technologies on self-efficacy presents new opportunities to improve knowledge and encourage people to donate blood.</div></div><div><h3>Objective</h3><div>A scoping review was conducted to understand the nature of the scientific evidence on improving knowledge and influencing the behaviours of blood donors with eHealth technologies.</div></div><div><h3>Methods</h3><div>Through a systematic literature search, 6261 published papers were screened, and 20 were reviewed.</div></div><div><h3>Results</h3><div>eHealth technologies commonly utilized were audio/telephone calls, text messages, emails, audio-visuals, mobile health and smartphone applications, custommade software applications and social media. They were mainly used to disseminate educational and motivational information, send reminders, and enhance the blood donation experience. They significantly impacted the knowledge and behaviours of blood donors, including increased blood donation rates and repeat blood donations, and enhanced blood donor awareness, motivation and behaviours. Facilitators for their effective utilization included personalisation, organisational support, user-centred design and interactivity, clarity and privacy, automation, and cultural acceptance. Barriers included technical, communication, usability, poor access, low awareness and operational limitations.</div></div><div><h3>Conclusions</h3><div>eHealth technologies could be integrated into traditional blood donor programs even though they are not a panacea for all blood donor recruitment challenges. Targeted research is needed to understand their long-term effectiveness and applicability. Future research should address gaps identified, particularly focusing on the impact of contextual differences and health literacy. Such research will promote the development of more effective and inclusive blood donor engagement programs and technologies.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104112"},"PeriodicalIF":1.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A serological and molecular study on a case of Rh blood group mosaicism","authors":"Mingwei Yin ,&nbsp;Xinyu Huang ,&nbsp;Jing Pian ,&nbsp;Jihua Ma ,&nbsp;Xiaozhen Hong ,&nbsp;Xuejun Chen ,&nbsp;Xianguo Xu","doi":"10.1016/j.transci.2025.104111","DOIUrl":"10.1016/j.transci.2025.104111","url":null,"abstract":"<div><h3>Background</h3><div>Mosaicism refers to the presence of two or more genetically different cell lines within a single individual, the majority of which are accidentally discovered through routine blood group identification. Here, we report one case of Rh blood group mosaicism in a 10-year-old Chinese male.</div></div><div><h3>Methods</h3><div>Serological tests for the Rh blood group were performed via gel microcolumn tests and saline methods. Mosaic red blood cells (RBCs) were quantified via flow cytometry. The G-banded karyotype was analyzed at a 550-band resolution. Short tandem repeat (STR) analysis was used to compare the allele repeats at specific loci in DNA between blood, buccal mucosal, and hair shaft samples.</div></div><div><h3>Results</h3><div>Mixed-field agglutination with anti-C and anti-e antibodies was identified in the gel microcolumn test. The proportion of those positive for the C antigen was 47.26 %, while that of the negative ones was 52.74 %, as calculated by flow cytometry, and the double populations were identified as DCcEe- and DccEE-phenotype RBCs by multiple centrifugations. Karyotype analysis revealed 46, XY in the proband. STR analysis revealed that additional paternal alleles were present at the D1S1656, CSF1PO, and Penta D loci.</div></div><div><h3>Conclusion</h3><div>An individual with natural Rh blood group mosaicism was identified.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104111"},"PeriodicalIF":1.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden in plain sight: Detecting rare naturally occurring anti-K antibody in a blood donor","authors":"Rutuja Baldota ,&nbsp;Brinda Kakkar ,&nbsp;Sanjiv Ketkar ,&nbsp;Manaswi Patil","doi":"10.1016/j.transci.2025.104109","DOIUrl":"10.1016/j.transci.2025.104109","url":null,"abstract":"<div><div>Red cell alloimmunisation is commonly reported among pregnant, transplant and multitransfused patients, whereas it is a fairly rare occurrence in the healthy general population. We report a rare case where we detected naturally occurring anti-K antibody in a healthy male whole blood donor during routine antibody screening.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 3","pages":"Article 104109"},"PeriodicalIF":1.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}