Zoological Studies最新文献

{"title":"Phylogeography and Genetic Structure of the Bush Cricket <i>Decma fissa</i> (Orthoptera, Tettigoniidae) in Southern China.","authors":"Qi Guo,&nbsp;Qi-Di Zhu,&nbsp;Zhi-Jun Zhou,&nbsp;Fu-Ming Shi","doi":"10.6620/ZS.2023.62-32","DOIUrl":"https://doi.org/10.6620/ZS.2023.62-32","url":null,"abstract":"<p><p><i>Decma fissa</i> is the most widely distributed species of the genus <i>Decma</i> occuring in southern China. This study presents the first phylogeographic work of <i>D. fissa</i> based on <i>COI</i>, <i>Cytb</i> and <i>ITS</i> sequence. We examined genetic diversity with <i>ITS</i> and mitochondrial sequence respectively, and phylogenetic work was based on the mitochondrial data. A high-level genetic diversity was revealed based on mitochondrial data but a low-level diversity was shown with <i>ITS</i> sequence. For the mitochondrial data, divergence time analysis displayed five lineages. Based on the Mantel test, geographic and genetic distances among <i>D. fissa</i> populations revealed a significant positive correlation. Bayesian skyline plot (BSP) analyses implied that none of three major lineages of <i>D. fissa</i> was seemingly affected by the last glacial maximum (LGM, 0.015-0.025 Mya). Ecological niche modeling was used to predict the distribution of <i>D. fissa</i> in four periods (LGM, Mid-Holocene, current and 2070) in China. Analysis of the ancestral area reconstruction indicated that <i>D. fissa</i> occurred in the South China area.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"62 ","pages":"e32"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475508/pdf/zoolstud-62-032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels (<i>Anguilla japonica</i>).","authors":"Hsiang-Yi Hsu,&nbsp;Chia-Hsien Chuang,&nbsp;I-Hsuan Lu,&nbsp;Chung-Yen Lin,&nbsp;Yu-San Han","doi":"10.6620/ZS.2023.62-02","DOIUrl":"https://doi.org/10.6620/ZS.2023.62-02","url":null,"abstract":"<p><p>The Japanese eel (<i>Anguilla japonica</i>) is an important species in East Asian aquaculture. However, the production of seedlings for this purpose still depends on natural resources, as the commercial production of glass eels is not yet possible. Confusion about the sex of silver eels is one of the factors affecting the success rate of artificial maturation. This study sought to devise a harmless method to precisely assess the sex of silver eels. Partial pectoral fins were collected from females and males and the total RNA was extracted for transcriptomic analysis to identify sexually dimorphic genes as molecular markers for sex typing. An online database was constructed to integrate the annotations of transcripts and perform comparative transcriptome analysis. This analysis identified a total of 29 candidate sexually dimorphic genes. Ten were selected for a real-time quantitative polymerase chain reaction (RT-qPCR) to validate the transcriptomic data and evaluate their feasibility as markers. The transcriptomic analysis and RT-qPCR data implicated three potential markers (<i>LOC111853410</i>, <i>kera</i>, and <i>dcn</i>) in sex typing. The expression of <i>LOC111853410</i> was higher in females than in males. In contrast, the expression of <i>kera</i> and <i>dcn</i> was higher in males than in females. The ΔC_T values of three markers were analyzed to determine their inferred thresholds, which can be used to determine the sex of Japanese eels. The results suggested that if a silver eel had a pectoral fin with the pectoral fin having the ΔC_T of <i>LOC111853410</i> < 11.3, the ΔC_T of <i>kera</i> > 11.4, or the ΔC_T of <i>dcn</i> > 6.5 can be assessed it could be assessed as female. Males could be assessed by the ΔC_T of <i>LOC111853410</i> > 11.3, the ΔC_T of <i>kera</i> < 11.4, or the ΔC_T of <i>dcn</i> < 6.5 in their pectoral fins. The molecular functions of these markers and the biological significance of their differential expression require further exploration.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"62 ","pages":"e2"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131072/pdf/zoolstud-62-002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9386608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tuerkayana latens</i>, a New Species of Land Crab from French Polynesia, with a Discussion on the Phylogeny of the Genus (Crustacea: Decapoda: Brachyura: Gecarcinidae).","authors":"Peter K L Ng,&nbsp;Hsi-Te Shih","doi":"10.6620/ZS.2023.62-10","DOIUrl":"https://doi.org/10.6620/ZS.2023.62-10","url":null,"abstract":"<p><p>The taxonomy of the gecarcinid land crab <i>Tuerkayana rotundum</i> (Quoy & Gaimard, 1824) is revised. The taxon, the type species of the genus, occurs from the western Indian Ocean to the western Pacific, and varies substantially in colour and various morphological features, but the male first gonopod structure is constant and the extensive genetic data from mitochondrial 16S rDNA, cytochrome <i>c</i> oxidase subunit 1 and control region markers confirms the recognition of one wide-ranging species. Specimens from Tuamotu in French Polynesia and Pitcairn Island, however, differ in having a smoother and slightly more swollen carapace, and significantly, the male first gonopod structure is distinctly different. The genetic data also supports their differentiation. As such, this material is here recognised as a new species, <i>Tuerkayana latens</i> n. sp.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"62 ","pages":"e10"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201346/pdf/zoolstud-62-010.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9521987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Distribution of Wedge Clams (Donacidae: Bivalvia) in Thailand by Geometric Morphometric and Molecular Analysis.","authors":"Cheewarat Printrakoon, Sasimanas Unajak, Javier H Signorelli","doi":"10.6620/ZS.2022.61-82","DOIUrl":"10.6620/ZS.2022.61-82","url":null,"abstract":"<p><p>The phylogenetic relationship of living Thai Donacidae was herein studied. Two methodologies, geometric morphometrics (GM) and genetic analysis of <i>COI</i> sequences, were combined and applied to identify the valid taxa and explain biodiversity and the distribution pattern in this family. A total of 587 living specimens were tested to analyze the shape and size patterns by Elliptic Fourier Analysis (EFA). Shell identification and GenBank sequences were added to construct the phylogenetic relationship and haplotype network. Centroid size was used to identify the specimens to the subgenus level. <i>Donax</i> (<i>Hecuba</i>) <i>scortum</i>, was easily distinguished from other species by Principal Component analysis (PCA) of shell size and shape. <i>Donax</i> (<i>Dentilatona</i>) <i>incarnatus</i> and <i>Donax</i> (<i>Deltachion</i>) <i>semisulcatus semisulcatus</i> were identified using Canonical Variates Analysis (CVA). Pairwise comparison of EFA was used for species level recognition, particularly shape overlap was observed for medium and small shell size. Based on genetic distance and haplotype network of <i>COI</i> sequences, <i>Donax</i> (<i>Latona</i>) <i>faba</i> and <i>D.</i> (<i>Latona</i>) <i>solidus</i> could be grouped in the same clade. Intraspecific and interspecific genetic data variation of some common species in different geographical localities of Thailand was observed. Three distribution patterns of <i>Donax species</i> were observed along the two-marine system of Thailand.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e82"},"PeriodicalIF":1.5,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076522/pdf/zoolstud-61-082.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Stolephorus lotus</i>, a New Anchovy (Teleostei: Clupeiformes: Engraulidae) from the Northern Territory, Australia.","authors":"Harutaka Hata, Hiroyuki Motomura","doi":"10.6620/ZS.2022.61-87","DOIUrl":"10.6620/ZS.2022.61-87","url":null,"abstract":"<p><p>A new anchovy <i>Stolephorus lotus</i> sp. nov., is described based on 30 specimens collected from Van Diemen Gulf, Northern Territory, Australia. The species closely resembles <i>Stolephorus acinaces</i> Hata, Lavoué and Motomura, 2020 and <i>Stolephorus andhraensis</i> Babu Rao, 1966, in having a long maxilla (posterior tip just reaching or extending slightly beyond the posterior margin of the opercle), indented posterior preopercular margin, anal fin with 16-18 branched fin rays, and 21-23 lower gill rakers, and lacking a predorsal scute and pelvic scute spine. However, the new species differs from the other two species in having higher counts of longitudinal series of scale rows and predorsal scales (37-39 and 20 or 21, respectively vs. 35-38 and 17-19 in the other two) and a more anteriorly located anal-fin origin (below bases of sixth to eighth dorsal-fin rays vs. eighth to tenth dorsal-fin rays).</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e87"},"PeriodicalIF":1.5,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061296/pdf/zoolstud-61-087.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9241225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrative Description of Two New <i>Mesobiotus</i> Species (Tardigrada: Eutardigrada: Macrobiotidae) with Updated Genus Phylogeny.","authors":"Daniel Stec","doi":"10.6620/ZS.2022.61-85","DOIUrl":"10.6620/ZS.2022.61-85","url":null,"abstract":"<p><p>This work presents two new <i>Mesobiotus</i> species from the Republic of South Africa, formally described using integrative analyses. Specimens of the new species are examined in terms of morphology and morphometry under a contrast phase light microscope (PCM) and scanning electron microscope (SEM). For both new species, genetic data in the form of DNA sequences of commonly used molecular markers are also provided (18S rRNA, 28S rRNA, <i>COI</i>, ITS-2). Furthermore, such genetic data are also provided for the first time for <i>Mesobiotus peterseni</i> (Maucci, 1991) from Greenland. The study also presents a multilocus molecular phylogeny of the genus and an elaborated discussion on the taxa groupings and species composition. This results in the ratification of three informal morpho-groups in order to ease and improve communication in further taxonomic studies on the genus. Finally, an updated key to all valid nominal <i>Mesobiotus</i> taxa (71 species) is provided to enhance species identification in this morphologically diverse group of limno-terrestrial tardigrades.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e85"},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061300/pdf/zoolstud-61-085.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9241226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematics and Biogeography of Fiddler Crabs -A Special Issue in <i>Zoological Studies</i>.","authors":"Hsi-Te Shih, Benny K K Chan","doi":"10.6620/ZS.2022.61-64","DOIUrl":"10.6620/ZS.2022.61-64","url":null,"abstract":"<p><p>Fiddler crabs are a fantastic group of intertidal brachyuran crabs, and the research fields focused on their biodiversity, phylogeography, phylogenomics, and larval biology are still in developing stages. In this special issue, seven articles are included focusing on the diversity, phylogeography, mitogenome phylogeny and larval morphology of fiddler crabs, covering the regions of the Indo-West Pacific and Americas. Results from this special issue open up further opportunities to study new species identification based on an integrative taxonomy approach, genomic-level phylogeny and larval morphology, especially in regards to the mitogenomes in the genera <i>Cranuca</i>, <i>Gelasimus</i>, <i>Paraleptuca</i>, and <i>Uca</i> for filling up the knowledge gap of fiddler crabs in the world.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e64"},"PeriodicalIF":1.5,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755984/pdf/zoolstud-61-064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10443687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Zoeal Stage of 15 Species of Fiddler Crabs (Crustacea: Brachyura: Ocypodidae) from Taiwan.","authors":"Ying-Chen Zhang, Hsi-Te Shih","doi":"10.6620/ZS.2022.61-71","DOIUrl":"10.6620/ZS.2022.61-71","url":null,"abstract":"<p><p>Fiddler crabs (Brachyura: Ocypodidae: Gelasiminae) in Taiwan consist of 5 genera and 15 species, but knowledge of their larval development is limited to just 3 species, namely <i>Austruca lactea</i>, <i>Tubuca arcuata</i>, and <i>Xeruca formosensis</i>. In our study, the morphology of the first zoeal stage (zoea I) of the 15 species is described and compared to previous studies. The results show that the characters of zoea I can be used to distinguish the five studied genera and most species (except three groups, \"<i>Gelasimus borealis</i>, <i>G. jocelynae</i> and <i>G. vocans</i>\", \"<i>Paraleptuca crassipes</i> and <i>P. splendida</i>\" and \"<i>Tubuca arcuata</i>, <i>T. coarctata</i> and <i>T. paradussumieri</i>\"). The lateral spines on the carapace in zoea I are suggested to be a unique character in the Ocypodinae because they are absent in the Gelasiminae and Ucidinae, which supports the current systematics of the Ocypodidae.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e71"},"PeriodicalIF":1.5,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9810989/pdf/zoolstud-61-071.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9085617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larval Development of the Mangrove Fiddler Crab <i>Austruca albimana</i> (Kossmann, 1877) (Crustacea: Brachyura: Ocypodidae) Under Laboratory Conditions.","authors":"Austin A J Kumar, Ali M Al-Aidaroos","doi":"10.6620/ZS.2022.61-70","DOIUrl":"10.6620/ZS.2022.61-70","url":null,"abstract":"<p><p>Larvae of the mangrove fiddler crab <i>Austruca albimana</i> (Kossmann, 1877), hatched from an ovigerous female collected from the mangroves of Sumariat, Jazan Province, Saudi Arabia in the southern Red Sea, were reared in the laboratory. Four zoeal and a megalopal stages were recorded, and their morphological features are described herein for the first time. The setations of the cephalothoracic appendages of the zoeas of <i>A. albimana</i> and their congeners exhibit several variations that help differentiate larvae of this genus easily from other meroplankton. However, a character of phylogenetic significance -minute spines on the forks of the telson of pleon -is common to larvae of this genus. These minute spines were studied with the aid of scanning electron microscope images. There were five common morphological features between <i>A. albimana</i> and other fiddler crab megalope, including <i>Minuca burgersi</i>, <i>Leptuca uruguayensis</i> and <i>Leptuca thayeri</i>. These features were a deflexed front, rounded to obtuse frontal margin, seven-segmented antennal flagellum, unsegmented endopod of maxilla and three cincinnuli on the endopods of pleopods. Two zoeal morphological features described in this study and other studies (<i>i.e.</i>, the absence of lateral spines on carapace [vs. their presence in species of <i>Uca</i>, <i>Afruca</i> and <i>Ocypode</i> in the Ocypodinae] and the presence of a maximum of four pairs of inner setae on the telson of pleon [vs. presence of more than four pairs of setae in species of <i>Uca</i>, <i>Afruca</i> and <i>Ocypode</i>]) support the taxonomic amendment of transferring <i>Uca</i> spp. and <i>Afruca</i> spp. crabs from Gelasiminae to Ocypodinae.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e70"},"PeriodicalIF":1.5,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9810992/pdf/zoolstud-61-070.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeography of the Fiddler Crab <i>Tubuca arcuata</i> (Crustacea: Brachyura: Ocypodidae) in East Asia and Northern Vietnam.","authors":"Hsi-Te Shih, Min-Yun Liu, Misuzu Aoki, Hiroshi Suzuki","doi":"10.6620/ZS.2022.61-68","DOIUrl":"10.6620/ZS.2022.61-68","url":null,"abstract":"<p><p>The fiddler crab <i>Tubuca arcuata</i> (Crustacea: Ocypodidae: Gelasiminae) is widely distributed across East Asia and northern Vietnam. Particularly, this species inhabits estuarine mangroves and mudflats of the East Asian continent, as well as the main islands of Japan and the Ryukyus. By comparing sequences of the mitochondrial 16S, cytochrome oxidase subunit I (<i>COI</i>), and control region genes, two main clades of this species were elucidated. The northern (N) clade was mostly restricted to the region north of the Taiwan Strait, whereas the wide (W) clade could be found throughout the entire range inhabited by this species. Based on the distribution of the ancestral haplotypes of <i>COI</i> and the divergence time of the two clades, our findings suggest that the land bridge barrier present in the Taiwan Strait during glaciations might have played an important role in their cladogenesis, approximately 0.93 million years ago. Here, we propose that the distribution of the ancestral haplotype of the W clade in the Ryukyus and the main islands of Japan was determined by the Paleo-Kuroshio Current, whereas the modern distribution pattern is shaped by the modern Kuroshio Current and other warm currents during the crab reproduction season in the summer.</p>","PeriodicalId":49331,"journal":{"name":"Zoological Studies","volume":"61 ","pages":"e68"},"PeriodicalIF":1.5,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755987/pdf/zoolstud-61-068.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10443688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}