Ticks and Tick-borne Diseases最新文献

{"title":"Various small mammal hosts are involved in the transmission cycle of zoonotic Anaplasma phagocytophilum in Southeastern Canada","authors":"Raphaëlle Audet-Legault ,&nbsp;Cécile Aenishaenslin ,&nbsp;Geneviève Baron ,&nbsp;Heather Coatsworth ,&nbsp;Marion Desmarchelier ,&nbsp;Vanessa Gabriele-Rivet ,&nbsp;Patrick A․ Leighton ,&nbsp;Jérôme Pelletier ,&nbsp;Jean-Philippe Rocheleau ,&nbsp;Catherine Bouchard","doi":"10.1016/j.ttbdis.2025.102551","DOIUrl":"10.1016/j.ttbdis.2025.102551","url":null,"abstract":"<div><div>Human granulocytic anaplasmosis (HGA) is a tick-borne disease emerging in North America, caused by the zoonotic variant Ap-ha (human active) of the bacterium <em>Anaplasma phagocytophilum.</em> This research aimed to understand the reservoir competency of small mammals for Ap-ha in the province of Québec, Canada. To achieve this aim, wild small mammals were sampled from June to August in 2022 and 2023 and tested for Ap and its variants (Ap-ha, Ap-v1) using qPCR targeting the <em>msp2</em> and 16S genes. In total, 547 small mammals from nine different taxa were live captured across two years<em>.</em> Nearly half (48.2 %) of small mammals carried at least one <em>Ixodes scapularis</em> tick larva and 29.3 % carried at least one <em>I. scapularis</em> nymph. The odds of Ap infection were three times higher in eastern chipmunks (OR = 2.98, <em>p</em> &lt; 0.05) and five times lower in red squirrels (OR = 0.21, <em>p</em> &lt; 0.01) when compared to <em>Peromyscus</em> spp. mice. When infected, <em>Peromyscus</em> mice and eastern chipmunks transmitted the infection on average to 6.8 % (CI95: 24.9–48.7) and 52.9 % (CI95: 16.7–89.1) of feeding larvae, respectively. An Ap variant could be identified in 90 % (109/121) of infected small mammals, all of which were the Ap-ha strain. In conclusion, eastern chipmunks and <em>Peromyscus</em> mice should be considered as reservoir hosts contributing to the transmission and emergence of anaplasmosis in Canada. This study also confirms the circulation of the Ap-ha variant within host and tick communities in Southeastern Canada.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102551"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145160093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Spatial distribution and clustering of medically important tick species in Illinois: Implications for tick-borne disease risk” [Ticks and Tick-borne Diseases 16 (2025) 102533]","authors":"Abrar Hussain ,&nbsp;Csaba Varga ,&nbsp;Brian F. Allan ,&nbsp;Nohra Mateus-Pinilla ,&nbsp;Rebecca L. Smith","doi":"10.1016/j.ttbdis.2025.102558","DOIUrl":"10.1016/j.ttbdis.2025.102558","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102558"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145330651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing disease surveillance in rhinoceroses: A multiplex real-time PCR assay for detecting Theileria bicornis and Babesia bicornis","authors":"Naledi P. Sekgobela ,&nbsp;Ilse Vorster ,&nbsp;Milana Troskie ,&nbsp;Melvyn Quan ,&nbsp;David E. Zimmerman ,&nbsp;Ayesha Hassim ,&nbsp;Luis Neves ,&nbsp;Raksha V. Bhoora","doi":"10.1016/j.ttbdis.2025.102552","DOIUrl":"10.1016/j.ttbdis.2025.102552","url":null,"abstract":"<div><div>Black (<em>Diceros bicornis</em>) and white (<em>Ceratotherium simum</em>) rhinoceroses in Southern Africa face multiple threats, including poaching, habitat loss, and translocation stress. Infections with <em>Theileria bicornis</em> (in both rhino species) and <em>Babesia bicornis</em> (confirmed only in black rhinos) add further health risks, with stressors such as translocation potentially increasing susceptibility. Effective management requires sensitive molecular diagnostic assays for accurate detection and surveillance. To address this, we developed a multiplex qPCR assay (MqTbBb) using species-specific TaqMan™ minor groove binder (MGB) probes for the simultaneous detection of <em>T. bicornis</em> and <em>B. bicornis</em>. The assay targets 18S rRNA gene regions, amplifying an 87 bp fragment for <em>T. bicornis</em> and a 51 bp fragment for <em>B. bicornis</em>, with efficiencies of 100 % and 98 %, respectively. Probit analysis determined a 95 % Limit of detection of 1.00 × 10⁻⁶ % and 6.27 × 10⁻⁶ % equivalent parasitized erythrocytes for <em>T. bicornis</em> and <em>B. bicornis</em>, respectively. No cross-reactivity was observed with other related protozoa tested. A total of 223 field samples from rhinos (101 black and 122 white) in Mpumalanga province were screened using both the MqTbBb and Reverse Line Blot (RLB) hybridization assays. The MqTbBb detected <em>T. bicornis</em> in 57 % of black and 99 % of white rhinos, with co-infections in 40 % of black rhinos. RLB detected <em>T. bicornis</em> in 96 % of black and 95 % of white rhinos, with a <em>Babesia</em> catch-all probe signal in 75 % and 32 %, respectively. <em>B. bicornis</em> was not detected by RLB and was never detected as a single infection by qPCR. These findings highlight high <em>T. bicornis</em> prevalence and rare <em>B. bicornis</em> infections (co-infections). The MqTbBb assay strengthens detection, surveillance, and conservation efforts.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102552"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen screening in ticks impacted by microbial reference genomes","authors":"Koray Ergunay ,&nbsp;Hein Sprong ,&nbsp;Yvonne-Marie Linton","doi":"10.1016/j.ttbdis.2025.102572","DOIUrl":"10.1016/j.ttbdis.2025.102572","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102572"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145574798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective control of severe fever with thrombocytopenia syndrome in Daishan County: Correspondence","authors":"Hinpetch Daungsupawong ,&nbsp;Viroj Wiwanitkit","doi":"10.1016/j.ttbdis.2025.102574","DOIUrl":"10.1016/j.ttbdis.2025.102574","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102574"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From import to establishment? Experimental evidence for seasonal outdoor survival of two Rhipicephalus species in Germany","authors":"K. Fachet-Lehmann,&nbsp;A. Lindau,&nbsp;U. Mackenstedt","doi":"10.1016/j.ttbdis.2025.102560","DOIUrl":"10.1016/j.ttbdis.2025.102560","url":null,"abstract":"<div><div>The brown dog tick (<em>Rhipicephalus sanguineus</em> s.l.), though not endemic in Germany, is regularly introduced via travelers with dogs and imported rescue dogs. Due to its relevance in veterinary and human medicine, its potential to establish in Germany’s climate is of interest. Although previous studies confirm indoor survival and reproduction of <em>R. sanguineus</em> s.s. in Germany, climate change and milder winters may also allow outdoor survival. This study assessed the survival of <em>R. sanguineus</em> s.s. and <em>R. innaei</em> from February 2023 to May 2024 using laboratory-bred ticks placed at indoor and outdoor sites. Tick survival (adults, nymphs, larvae) was monitored weekly, along with temperature and humidity. Reproductive success was evaluated via oviposition and larval hatching.</div><div><em>R. sanguineus</em> s.s. adults survived up to 44 weeks, nymphs up to 20 weeks, and larvae up to 5 weeks. <em>R. innaei</em> showed shorter survival (37, 10, and 4 weeks, respectively)<em>.</em> Successful oviposition and larval hatching occurred outdoors between May´23 and September´23 for both species. However, winter survival was not observed; all ticks died following sub-zero temperatures in December´23.</div><div>Despite the inability to overwinter outdoors, both species can survive for extended periods in spring and summer and may enter homes via dogs, where conditions favor year-round survival. Their ability to transition indoors via dogs, where conditions favor year-round survival, suggests a potential for establishment in Germany through combined indoor and seasonal outdoor persistence.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102560"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “First molecular detection of Anaplasma marginale, Babesia bovis, and hemotropic Mycoplasma spp. in bovine colostrum” [Ticks and Tick-borne Diseases 16(6) (2025) 102553]","authors":"Mayara Campos Lombardi ,&nbsp;Nicolas Colácio ,&nbsp;Camila de Valgas e Bastos ,&nbsp;Sandra Gesteira Coelho ,&nbsp;Tiago Facury Moreira ,&nbsp;Rodrigo Melo Meneses ,&nbsp;Antônio Último de Carvalho ,&nbsp;Elias Jorge Facury-Filho ,&nbsp;Júlia Angélica Gonçalves Silveira","doi":"10.1016/j.ttbdis.2025.102559","DOIUrl":"10.1016/j.ttbdis.2025.102559","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102559"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145373215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Borrelia miyamotoi and Neoehrlichia mikurensis in a cohort of tick-exposed individuals and population controls in Denmark 2002–2021","authors":"Helene Mens ,&nbsp;Rosa Gynthersen ,&nbsp;Nanna Skaarup Andersen ,&nbsp;Mathilde Ørbæk ,&nbsp;Fredrikke C. Knudtzen ,&nbsp;Sanne Løkkegaard Larsen ,&nbsp;Sigurdur Skarphedinsson ,&nbsp;Olga A. Stukolova ,&nbsp;Dieuwertje Hoornstra ,&nbsp;Joppe W. Hovius ,&nbsp;Anne-Mette Lebech","doi":"10.1016/j.ttbdis.2025.102557","DOIUrl":"10.1016/j.ttbdis.2025.102557","url":null,"abstract":"<div><div><em>Borrelia miyamotoi</em> and <em>Neoehrlichia mikurensis</em> are recently discovered tick-borne pathogens. The aim of this study was to ascertain the extent of transmission among high-risk groups and healthy controls.</div><div>The study design is retrospective cohort study. Blood from a total of 1180 individuals, collected between 2002–2021: (i) 180 tick-exposed individuals, (ii) 400 immunocompromised individuals and (iii) 600 blood donors was included. Infection was examined by a protein array (<em>B. miyamotoi</em>) and PCR.</div><div><em>Neoehrlichia mikurensis</em> DNA was detected in only one individual with Lyme neuroborreliosis (LNB) with symptoms compatible with both infections. <em>Borrelia miyamotoi</em> seroprevalence (being either IgM or IgG positive) among tick-exposed individuals (8.3 %, 95%CI 5.1–13.3) was significantly higher compared to healthy blood donors (1.5 %, 95 % CI 0.8–2.8) and immunocompromised individuals (3.3 %, 95 %CI 1.9–5.5), <em>p</em> &lt; 0.0001. Altogether, 37 of 1180 individuals (3.1 %, 95 %CI 2.2–4.3) were found to be <em>B. miyamotoi</em> seropositive.</div><div>Neoehrlichiosis and <em>Borrelia miyamotoi</em> disease should be considered in patients with febrile illness and relevant exposure and in non-characteristic presentations of Lyme borreliosis in Denmark.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102557"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tickborne microorganisms in Dermacentor andersoni (Acari: Ixodidae) in the Bitterroot Mountains of Western Montana","authors":"K.L. Clark ,&nbsp;J. Villegas Nunez ,&nbsp;C. Gentry ,&nbsp;A. Gibson ,&nbsp;H. Husein ,&nbsp;R. Burstein ,&nbsp;K. Hamm","doi":"10.1016/j.ttbdis.2025.102555","DOIUrl":"10.1016/j.ttbdis.2025.102555","url":null,"abstract":"<div><div>The Rocky Mountain wood tick, <em>Dermacentor andersoni</em> Stiles, is the most common human biting tick in the Rocky Mountain region of the USA. Although <em>Rickettsia</em> spp. and Colorado tick fever virus have been studied in <em>D. andersoni</em> in certain areas of their distribution, investigations of most other tickborne pathogens are lacking. Despite the history of Rocky Mountain spotted fever in the Bitterroot Mountains of western Montana, no comprehensive surveys of large numbers of ticks for <em>Rickettsia</em> spp. have been conducted there for &gt;40 years. For the present study, host-seeking adult <em>D. andersoni</em> were collected at multiple sites in canyons on the west side of the Bitterroot Valley in western Montana in spring 2021. Following DNA extraction, ticks were screened by polymerase chain reaction assays for several tick-borne microorganisms. DNA sequence data analysis was conducted to confirm identity and conduct phylogenetic comparisons with reference strain sequences of different organisms. No ticks were found to contain <em>Anaplasma</em> spp., <em>Borrelia</em> spp., or <em>Ehrlichia</em> spp. DNA, and a <em>Babesia</em> sp. was detected in only one tick. However, <em>Bartonella</em> spp. DNA was detected in 2 % of tested ticks. Additionally, five species of <em>Rickettsia</em>, including <em>R. peacockii, R. bellii, R. rhipicephali, R. montanensis</em>, and <em>R. rickettsii,</em> were identified in ticks from different sites. The most prevalent <em>Rickettsia</em> species was <em>R. peacockii</em> and the least common was <em>R. rickettsii</em> (found in only two ticks). The relevance of these findings is discussed in relation to historical and contemporary data on <em>Bartonella</em> spp. and <em>Rickettsia</em> spp.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102555"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reply: Response to “Effective control of severe fever with thrombocytopenia syndrome in Daishan County”","authors":"Jiangping Ren","doi":"10.1016/j.ttbdis.2025.102576","DOIUrl":"10.1016/j.ttbdis.2025.102576","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102576"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145702757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}