Ticks and Tick-borne Diseases最新文献

{"title":"Repeated imidocarb treatment failure suggesting emerging resistance of Babesia canis in a new endemic area in north-eastern Germany","authors":"Christiane Weingart ,&nbsp;Jürgen Krücken ,&nbsp;Barbara Kohn","doi":"10.1016/j.ttbdis.2024.102315","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102315","url":null,"abstract":"<div><p>Canine babesiosis has been increasingly diagnosed in various regions of Germany such as north-eastern Germany in recent years. A dog with several relapses of <em>Babesia canis</em> infection after treatment with imidocarb is described. A 9-year-old male Magyar Viszla with <em>B. canis</em> infection was referred after two treatments with imidocarb (dosage 2.1 mg/kg SC) because of lethargy, fever and pancytopenia (additional treatments with prednisolone and doxycycline). Merozoites were detected in the blood smear and imidocarb treatment was repeated. Clinical signs, pancytopenia and a positive <em>B. canis</em> PCR occurred after the 3rd (6 mg/kg SC), 4th (7.7 mg/kg SC) and 5th (7.5 mg/kg SC and doxycycline for 4 weeks in addition) imidocarb injection and thorough tick prevention with isoxazoline and permethrin products. 12 days after the 5th injection, the PCR was negative for the first time. The dog was again presented with fever 35 days after the 5th injection. The <em>B. canis</em> PCR was positive and laboratory examination revealed pancytopenia. Treatment with atovaquone/azithromycin for 18 days was performed and no further relapse occurred for 32 weeks. In the case of suspected imidocarb resistance in <em>B. canis</em> infection, treatment with atovaquone/azithromycin can be an alternative.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 3","pages":"Article 102315"},"PeriodicalIF":3.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000086/pdfft?md5=1c00246819b60a849fd98936e2feb5e6&pid=1-s2.0-S1877959X24000086-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139654096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A brief tale of two pioneering moments: Europe's first discovery of Tick-Borne Encephalitis (TBE) virus beyond the Soviet Union and the largest alimentary TBE outbreak in history","authors":"Daniel Ruzek ,&nbsp;Kristyna Kaucka","doi":"10.1016/j.ttbdis.2024.102314","DOIUrl":"10.1016/j.ttbdis.2024.102314","url":null,"abstract":"<div><p>The emergence of tick-borne encephalitis (TBE) in Europe marked several significant milestones. The discovery of TBE in Czechoslovakia in 1948, with Gallia and Krejčí simultaneously isolating the TBE virus (TBEV) from human samples for the first time in Europe outside the Soviet Union, was pivotal. Subsequent TBEV isolation from ticks suggested the viral transmission via this vector. In 1951, the outbreak in Rožňava in Slovakia (Czechoslovakia) revealed an unexpected mode of transmission, unpasteurized milk from a local dairy, challenging existing understanding. Investigations exposed illicit practices of mixing cow's milk with goat's milk for economic gains. Laboratory research confirmed the outbreak was caused by TBEV, which was substantiated by serological analyses. This was the first and largest documented alimentary TBE outbreak in history. In this review, we delve into both published sources and unpublished archival data, offering a comprehensive understanding of these historic accomplishments and shedding light on these pivotal moments.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 3","pages":"Article 102314"},"PeriodicalIF":3.2,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000074/pdfft?md5=682a352a98738030020a6a5243d0925e&pid=1-s2.0-S1877959X24000074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139582177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks","authors":"Andressa Aparecida de Lima Reis ,&nbsp;Barbara Rauta de Avelar ,&nbsp;Marisa Beatriz da Silva Rocha ,&nbsp;Debora Azevedo Borges ,&nbsp;Diefrey Ribeiro Campos ,&nbsp;Jessica Fiorotti ,&nbsp;Patrícia Silva Golo ,&nbsp;Fabio Barbour Scott","doi":"10.1016/j.ttbdis.2024.102312","DOIUrl":"10.1016/j.ttbdis.2024.102312","url":null,"abstract":"<div><p><em>Amblyomma sculptum</em> (formerly <em>Amblyomma cajennense</em>) ticks have been implicated in the transmission of pathogens that cause diseases in animals and humans. Their wide geographic distribution and high impact on animal health and zoonotic disease transmission highlight the importance of studying and implementing effective control measures to mitigate the risks associated with this tick species. The aim of this study was to quantify and characterize the morphology and the ultrastructure of different types of hemocytes in the hemolymph in engorged <em>A. sculptum</em> females fed on rabbits. The hemolymph samples were collected by perforation of the cuticle in the dorsal region. Hemocyte types, sizes, and differential counts were determined using light microscopy, while ultrastructural analysis of hemocytes was performed using transmission electron microscopy. The average number of total hemocytes in the hemolymph was 1024 ± 597.6 cells µL⁻¹. Five morphologically distinct cell types were identified in <em>A. sculptum</em> females: prohemocytes (6 % ± 8.8), plasmatocytes (10 % ± 7.7), granulocytes (78 % ± 12.2), spherulocytes (5 % ± 4.48), and oenocytoids (1 % ± 1.6). In general, prohemocytes were the smallest hemocytes. The ultrastructural morphology of <em>A. sculptum</em> hemocytes described in the present study agrees with the findings for other hard ticks. This is the first study to investigate ultrastructural characteristics of hemocytes of female <em>A. sculptum</em> ticks.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 3","pages":"Article 102312"},"PeriodicalIF":3.2,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000050/pdfft?md5=ff423a70a90f02d70be1da87a35b3eb8&pid=1-s2.0-S1877959X24000050-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hard ticks (Acari: Ixodidae) parasitizing bushbabies (Mammalia: Galagidae) in a biodiversity hotspot of northern South Africa","authors":"Ali Halajian ,&nbsp;Frank P. Cuozzo ,&nbsp;Heloise Heyne ,&nbsp;Michelle L. Sauther ,&nbsp;Birthe Linden ,&nbsp;Jabu Linden ,&nbsp;Adrian SW. Tordiffe ,&nbsp;Kgethedi Michael Rampedi ,&nbsp;Sándor Hornok","doi":"10.1016/j.ttbdis.2024.102313","DOIUrl":"10.1016/j.ttbdis.2024.102313","url":null,"abstract":"<div><p>South Africa has six species of primates, three of which are bushbabies (family Galagidae). Very little information is available on their parasites due to the lack of longitudinal studies, although <em>Rhipicephalus appendiculatus, Amblyomma hebraeum</em> and <em>Haemaphysalis elliptica</em> were previously reported from the brown greater galago (<em>Otolemur crassicaudatus</em>) in South Africa. During 2014–2019, 83 <em>O. crassicaudatus</em> (70 live-trapped and 13 deceased animals) were checked for the presence of hard ticks, all from Limpopo Province, South Africa. Seventy-three of 83 (88 %) galagos were found to be tick-infested. Among ixodid genera, <em>Haemaphysalis</em> had the highest prevalence (46 % of the bushbabies), followed by <em>Rhipicephalus</em> (25 %) and <em>Ixodes</em> (18 %). In total, ten tick species were identified. Importantly, all infestations were monospecific. Ticks occurred on various body parts of bushbabies, thus no predilection site was noted. In conclusion, while previously only three ixodid species were known to infest bushbabies in South Africa, the present study showed that these animals can be parasitized by a much broader range of hard ticks.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102313"},"PeriodicalIF":3.2,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000062/pdfft?md5=3231d8b02b745946f4ca0f949c1a0d3b&pid=1-s2.0-S1877959X24000062-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae)","authors":"Wenqiang Tang ,&nbsp;Xin Li ,&nbsp;Bijin Ye ,&nbsp;Bin Shi ,&nbsp;Haoji Zhang ,&nbsp;Zhisheng Dang ,&nbsp;Yuexiang Sun ,&nbsp;Lamu Danqu ,&nbsp;Chenyang Xia ,&nbsp;Danzeng Quzhen ,&nbsp;Xialing Zhao ,&nbsp;Wenting Chui ,&nbsp;Fuqiang Huang","doi":"10.1016/j.ttbdis.2024.102311","DOIUrl":"10.1016/j.ttbdis.2024.102311","url":null,"abstract":"<div><p>Ticks are specialized ectoparasites that feed on blood, causing physical harm to the host and facilitating pathogen transmission. The genus <em>Haemaphysalis</em> contains vectors for numerous infectious agents. These agents cause various diseases in humans and animals. Mitochondrial genome sequences serve as reliable molecular markers, forming a crucial basis for evolutionary analyses, studying species origins, and exploring molecular phylogeny. We extracted mitochondrial genome from the enriched mitochondria of <em>Haemaphysalis tibetensis</em> and obtained a 14,714-bp sequence. The mitochondrial genome consists of 13 protein-coding genes (PCGs), two ribosomal RNA, 22 transfer RNAs (tRNAs), and two control regions. The nucleotide composition of <em>H. tibetensis</em> mitochondrial genome was 38.38 % for A, 9.61 % for G, 39.32 % for T, and 12.69 % for C. The A + T content of <em>H. tibetensis</em> mitochondrial genome was 77.7 %, significantly higher than the G + C content. The repeat units of <em>H. tibetensis</em> exhibited two identical repeat units of 33 bp in length, positioned downstream of <em>nad1</em> and <em>rrnL</em> genes. Furthermore, phylogenetic analyses based on the 13 PCGs indicated that <em>Haemaphysalis tibetensis</em> (subgenus <em>Allophysalis</em>) formed a monophyletic clade with <em>Haemaphysalis nepalensis</em> (subgenus <em>Herpetobia</em>) and <em>Haemaphysalis danieli</em> (subgenus <em>Allophysalis</em>). Although the species <em>Haemaphysalis inermis, Haemaphysalis kitaokai, Haemaphysalis kolonini</em>, and <em>Haemaphysalis colasbelcouri</em> belong to the subgenus <em>Alloceraea</em>, which were morphologically primitive hemaphysalines just like <em>H. tibetensis</em>, these four tick species cannot form a single clade with <em>H. tibetensis</em>. In this study, the whole mitochondrial genome sequence of <em>H. tibetensis</em> from Tibet was obtained, which enriched the mitochondrial genome data of ticks and provided genetic markers to study the population heredity and molecular evolution of the genus <em>Haemaphysalis</em>.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102311"},"PeriodicalIF":3.2,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000049/pdfft?md5=d0ca4ae259c5ff579f431050a3516164&pid=1-s2.0-S1877959X24000049-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of field tropical Theileriosis and associated risk factors in two bioclimatic areas of Algeria","authors":"Tahar Kernif ,&nbsp;Bachir Medrouh ,&nbsp;Zoubir Harrat ,&nbsp;Fairouz Saidi ,&nbsp;Hocine Ziam","doi":"10.1016/j.ttbdis.2024.102310","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102310","url":null,"abstract":"<div><p>Tropical theileriosis (TT) is a tick-borne disease caused by Theileria annulata and commonly infects cattle in tropical and subtropical regions, including Algeria. It is a significant obstacle to cattle breeding programs established to improve production in Algeria. The present investigation aimed to estimate the current molecular prevalence, risk factors, and genetic characterisation of T. annulata in two bioclimatic areas of Algeria. In a cross-sectional study, 679 blood samples (629 from healthy cattle selected on farms and 50 from diseased cattle identified by veterinarians) were collected from the humid (<em>n</em> = 307+50) and semi-arid (<em>n</em> = 322) areas and screened by blood smear examination followed by polymerase chain reaction targeting cytochrome oxidase subunit 3 (cox III) mitochondrial and the 18S ribosomal RNA (18S rRNA) genes for Theileria spp. Seventy-six positive samples (56 clinically healthy and 20 with clinical signs) for Theileria spp. were confirmed to be T. annulata by the merozoïtes surface antigen-1 (Tams1) gene showing a rate of 8.9 % in clinically healthy and 40.0 % in suspected cattle. Among the 307 bloods samples collected from healthy cattle in the humid area, 25 cattle (8.1 %) were positive for T. annulata. Of the 322 healthy cattle from the semi-arid site, 31 (9.6 %) were carriers of T. annulata DNA. In subclinical population, demographic and environmental parameters analysis indicated that T. annulata infection was higher in adult crossbred cattle raised in the intensive and semi-intensive system (<em>P</em>&lt;0.001). The multiple logistic regression analysis showed that age, breed, farming system, and bioclimatic area are potential risk factors for T. annulata infection in cattle (<em>P</em>&lt;0.05). Multiple alignments of cox III sequences of T. annulata showed high heterogeneity with 25 polymorphic sites (nucleotide diversity π = 0.02402), resulting in two haplotypes with a low genetic diversity index (Hd) of 0.533. The 18S rRNA sequence alignment revealed only one T. annulata genotype with 100 % identity to the strains isolated from cattle and ticks in Mediterranean and Asian countries. Our preliminary results will serve as a basis for further studies on the genetic diversity and molecular epidemiology of T. annulata.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102310"},"PeriodicalIF":3.2,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000037/pdfft?md5=fabd1673f44506906030f9411b7f50f1&pid=1-s2.0-S1877959X24000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139493390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro acaricidal activity of essential oils and their binary mixtures against ixodes scapularis (Acari: Ixodidae)","authors":"Luís Adriano Anholeto ,&nbsp;Sophia Blanchard ,&nbsp;Haozhe Vincent Wang ,&nbsp;Ana Carolina de Souza Chagas ,&nbsp;Neil Kirk Hillier ,&nbsp;Nicoletta Faraone","doi":"10.1016/j.ttbdis.2024.102309","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102309","url":null,"abstract":"<div><p><em>Ixodes scapularis</em> ticks are vectors of infectious agents that cause illness in humans, including Lyme disease. Recent years have seen a surge in tick-borne diseases (TBD) resulting in a high demand for tick management products. Plants offer a valuable source of active compounds for the development of novel, eco-friendly tick control products, reducing potential risks to human and animal health. Essential oils (EOs) have emerged as potential acaricides and repellents against ticks providing an alternative to synthetic chemicals and aiding in the prevention of TBD by lowering the risk of tick bites. We investigated the acaricidal activity of EOs from lemongrass (C<em>ymbopogon citratus</em>), geranium (<em>Pelargonium x asperum</em>), savory thyme (<em>Thymus saturejoides</em>), and white thyme (<em>Thymus zygis</em>) on <em>I. scapularis</em>. The interactions (i.e., synergistic, antagonistic, or additive) of their binary mixtures were also evaluated. EO samples were analyzed via gas chromatography-mass spectrometry to determine their chemical composition. The adult immersion test was used to determine the lethal concentration (LC₅₀) of each EO alone and in mixtures. Quantitative assessment of synergistic, additive, or antagonistic effect of the binary mixtures was performed by calculating the combination index. Strong acaricidal activity was recorded for savory thyme and white thyme EOs, with LC₅₀ values of 28.0 and 11.0 μg/μL, respectively. The LC₅₀ of lemongrass and geranium EOs were 49.0 and 39.7 μg/μL, respectively. Among the tested EOs, savory thyme and white thyme had a strong acaricidal effect on <em>I. scapularis</em>, which might be linked to the presence of carvacrol (26.05 % ± 0.38) and thymol (53.6 % ± 2.31), main components present in savory thyme and white thyme EOs, respectively. The tick killing efficacy of lemongrass and geranium EOs was lower when mixed than when used separately (LC₅₀ of 65.3 µg/µL). The same happened with savory thyme and white thyme EOs, except at 9.75 µg/µL where they had a synergistic effect (LC₅₀ of 58.3 µg/µL). Lemongrass and savory thyme EOs had a synergistic effect at low concentrations, and an antagonistic effect at higher concentrations (LC₅₀ of 95.4 µg/µL). Lemongrass and white thyme EOs had a synergistic effect against ticks from 15 to 120 µg/µL (LC₅₀ of 18.5 µg/µL) similar to white thyme EO. Geranium and savory thyme EOs had an antagonistic effect at all concentrations, with an LC₅₀ of 66.8 µg/µL. Geranium and white thyme EOs also had an antagonistic effect, except at 12.7 µg/µL where they had a synergistic effect (LC₅₀ of 66.8 µg/µL). The interaction observed when combining selected essential oils suggests promising potential for developing acaricidal formulations aimed at controlling ticks and curbing the transmission of tick-borne disease agents.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102309"},"PeriodicalIF":3.2,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000025/pdfft?md5=8ac31fe299972a7d2641f12cbe5428c9&pid=1-s2.0-S1877959X24000025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139436502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Male C57BL/6J mice have higher presence and abundance of Borrelia burgdorferi in their ventral skin compared to female mice","authors":"Cody W. Koloski ,&nbsp;Georgia Hurry ,&nbsp;Alexandra Foley-Eby ,&nbsp;Hesham Adam ,&nbsp;Savannah Goldstein ,&nbsp;Pini Zvionow ,&nbsp;Susan E. Detmer ,&nbsp;Maarten J. Voordouw","doi":"10.1016/j.ttbdis.2024.102308","DOIUrl":"10.1016/j.ttbdis.2024.102308","url":null,"abstract":"<div><p><em>Borrelia burgdorferi</em> is a tick-borne spirochete that causes Lyme disease in humans. The host immune system controls the abundance of the spirochete in the host tissues. Recent work with immunocompetent <em>Mus musculus</em> mice strain C3H/HeJ found that males had a higher tissue infection prevalence and spirochete load compared to females. The purpose of this study was to determine whether host sex and acquired immunity interact to influence the prevalence and abundance of spirochetes in the tissues of the commonly used mouse strain C57BL/6. Wildtype (WT) mice and their SCID counterparts (C57BL/6) were experimentally infected with <em>B. burgdorferi</em> via tick bite. Ear biopsies were sampled at weeks 4, 8, and 12 post-infection (PI) and five tissues (left ear, ventral skin, heart, tibiotarsal joint of left hind leg, and liver) were collected at necropsy (16 weeks PI). The mean spirochete load in the tissues of the SCID mice was 260.4x higher compared to the WT mice. In WT mice, the infection prevalence in the ventral skin was significantly higher in males (40.0 %) compared to females (0.0 %), and the spirochete load in the rear tibiotarsal joint was significantly higher (4.3x) in males compared to females. In SCID mice, the spirochete load in the ventral skin was 200.0x higher in males compared to females, but there were no significant sex-specific difference in spirochete load in the other tissues (left ear, heart, tibiotarsal joint, or liver). Thus, the absence of acquired immunity greatly amplified the spirochete load in the ventral skin of male mice. It is important to note that the observed sex-specific differences in laboratory mice cannot be extrapolated to humans. Future studies should investigate the mechanisms underlying the male bias in the abundance of <em>B. burgdorferi</em> in the mouse skin.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102308"},"PeriodicalIF":3.2,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000013/pdfft?md5=06c8660c26389d5a1a73e954ae1e6a44&pid=1-s2.0-S1877959X24000013-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of transcription 1 hinders the replication of Dabie bandavirus by promoting the expression of TLR7, TLR8, and TLR9 signaling pathway","authors":"Hao An,&nbsp;Xiaoli Yu,&nbsp;Yumei Liu,&nbsp;Lei Fang,&nbsp;Ming Shu,&nbsp;Qingfeng Zhai,&nbsp;Junhao Chen","doi":"10.1016/j.ttbdis.2023.102307","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2023.102307","url":null,"abstract":"<div><p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is a bunyavirus that causes SFTS, with a case fatality rate of up to 30 %. The innate immune system plays a crucial role in the defense against SFTSV; however, the impact of viral propagation of STFSV on the innate immune system remains unclear. Although proteomics analysis revealed that the expression of the downregulator of transcription 1 (DR1) increased after SFTSV infection, the specific change trend and the functional role of DR1 during viral infection remain unelucidated. In this study, we demonstrate that DR1 was highly expressed in response to SFTSV infection in HEK 293T cells using qRT-PCR and Western blot analysis. Furthermore, viral replication significantly increased the expression of various TLRs, especially TLR9. Our data indicated that DR1 positively regulated the expression of TLRs in HEK 293T cells, DR1 overexpression highly increased the expression of numerous TLRs, whereas RNAi-mediated DR1 silencing decreased TLR expression. Additionally, the myeloid differentiation primary response gene 88 (MyD88)-dependent or TIR-domain-containing adaptor inducing interferon-β (TRIF)-dependent signaling pathways were highly up- and downregulated by the overexpression and silencing of DR1, respectively. Finally, we report that DR1 stimulates the expression of TLR7, TLR8, and TLR9, thereby upregulating the TRIF-dependent and MyD88-dependent signaling pathways during the SFTSV infection, attenuating viral replication, and enhancing the production of type I interferon and various inflammatory factors, including IL-1β, IL-6, and IL-8. These results imply that DR1 defends against SFTSV replication by inducing the expression of TLR7, TLR8, and TLR9. Collectively, our findings revealed a novel role and mechanism of DR1 in mediating antiviral responses and innate immunity.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102307"},"PeriodicalIF":3.2,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X23001887/pdfft?md5=5151809683775a58b23269b9ff159af8&pid=1-s2.0-S1877959X23001887-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139398773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tick-borne diseases in the North Sea region–A comprehensive overview and recommendations for diagnostics and treatment","authors":"Randi Eikeland ,&nbsp;Anna J. Henningsson ,&nbsp;Anne-Mette Lebech ,&nbsp;Yvonne Kerlefsen ,&nbsp;Sally Mavin ,&nbsp;Amber Vrijlandt ,&nbsp;Joppe W. Hovius ,&nbsp;Tinne Lernout ,&nbsp;Chin Lim ,&nbsp;Gerhard Dobler ,&nbsp;Volker Fingerle ,&nbsp;Rosa M. Gynthersen ,&nbsp;Per-Eric Lindgren ,&nbsp;Harald Reiso","doi":"10.1016/j.ttbdis.2023.102306","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2023.102306","url":null,"abstract":"<div><p>As part of the NorthTick project, co-funded by the European Union through the European Regional Development Fund and the North Sea Region Programme, specialists in the field of tick-borne diseases from seven North Sea countries co-operated with patient organisations and governmental health care institutions to provide this comprehensive overview of diagnostics and treatment recommendations in the region for Lyme borreliosis, <em>Borrelia miyamotoi</em> infection, tick-borne encephalitis, human granulocytic anaplasmosis, rickettsiosis, neoehrlichiosis and babesiosis. The main conclusion is that the recommendations in these northern countries are essentially the same, with very few differences. This overview presents the current diagnostics and provides useful clinical guidance.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 2","pages":"Article 102306"},"PeriodicalIF":3.2,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X23001875/pdfft?md5=34a87ae61f09ac47b46e73a5378499a1&pid=1-s2.0-S1877959X23001875-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139108429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}