Ticks and Tick-borne Diseases最新文献

{"title":"Evaluation of tick salivary and midgut extracellular vesicles as anti-tick vaccines in White-tailed deer (Odocoileus virginianus).","authors":"Julia Gonzalez, Cristina Harvey, Cárita de Souza Ribeiro-Silva, Brenda Leal-Galvan, Kelly A Persinger, Pia U Olafson, Tammi L Johnson, Adela Oliva Chavez","doi":"10.1016/j.ttbdis.2024.102420","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102420","url":null,"abstract":"<p><p>Current tick control measures are focused on the use of synthetic acaricides and personal protective measures. However, the emergence of acaricide resistance and the maintenance of tick populations in wildlife has precluded the efficient management of ticks. Thus, host-targeted, non-chemical control measures are needed to reliably reduce ticks parasitizing sylvatic reservoirs. This project aimed to evaluate extracellular vesicles (EVs) from Amblyomma americanum as vaccine candidates for white-tailed deer (Odocoileus virginianus; WTD). Salivary gland (SG) and midgut (MG) EVs were isolated by ultracentrifugation. Three deer were vaccinated with SG and MG EVs and received two boosters at days 28 and 50. Two control deer were injected with adjuvant and PBS only. On day 58, WTD were infested with 100 A. americanum nymphs, 50 females, and 50 males that were allowed to feed to repletion. On-host and off-host mortality, tick engorgement weight, nymph molting, time to oviposition, and egg hatchability were evaluated. Serum samples were recovered every seven days until the last day of tick drop off, and then at one year (Y1) and 1-year and 1-month (Y1M1). Vaccination resulted in seroconversion and significant increases in total IgG levels that remained significantly higher than controls and pre-vaccination levels at Y1 and Y1M1. No negative effects were observed in nymphs, but on-host mortality of female A. americanum was significantly higher in vaccinated animals. No effects were observed on reproductive parameters. These results indicate that proteins within female tick SG and MG vesicles are not good candidates for vaccine design against nymphs; however, the on-host adult mortality suggests that tick EVs harbor protective antigens against A. americanum females.</p>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 1","pages":"102420"},"PeriodicalIF":3.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board reshuffle at Ticks and Tick-borne Diseases.","authors":"Olaf Kahl, Ben Mans, Ard M Nijhof","doi":"10.1016/j.ttbdis.2024.102414","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102414","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":" ","pages":"102414"},"PeriodicalIF":3.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequence of Ehrlichia muris from Ixodes ricinus collected in Italy on a migratory bird provides epidemiological and evolutionary insights","authors":"Carlo Croci ,&nbsp;Luca Erriquez ,&nbsp;Beatrice Bisaglia ,&nbsp;Greta Bellinzona ,&nbsp;Emanuela Olivieri ,&nbsp;Davide Sassera ,&nbsp;Michele Castelli","doi":"10.1016/j.ttbdis.2024.102409","DOIUrl":"10.1016/j.ttbdis.2024.102409","url":null,"abstract":"<div><div>Ticks are prominent vectors of several zoonotic diseases. Tick-borne pathogens include the members of the genus <em>Ehrlichia</em>, which are obligate intracellular bacteria infecting immune and hematopoietic cells. <em>Ehrlichia muris</em> predominantly affects rodents, but was also reported to be a human pathogen. The known geographical distribution of this bacterium ranges from Asia, to the USA and eastern Europe. In the present work, we report the finding of <em>E. muris</em> in an <em>Ixodes ricinus</em> tick collected from a migratory bird (<em>Turdus iliacus</em>) in Italy, southern Europe. We sequenced the total DNA from this tick sample, and, thanks to a dedicated bioinformatic pipeline, selectively assembled the genome of the bacterium, which represents the first one for <em>E. muris</em> from Europe. Phylogenetic and comparative genomic analyses were then performed. Accounting for tick species distribution, bird migratory routes, and molecular phylogeny of the bacterium, it is likely that this bird transported the tick to Italy from an endemic area of <em>E. muris</em>, such as eastern Europe.</div><div>In addition, comparative genomic analyses highlighted that <em>E. muris</em> and other <em>Ehrlichia</em> spp. display copy number variations in two families of membrane proteins, likely due to recent gene duplication, deletion and recombination events. These differences are probably a source of variability for surface antigens to evade host immunity, with a potential role in host adaptation and specificity. The present results underline the impact of migratory birds on the spread of tick-borne pathogens towards non-endemic areas, highlighting the need for further epidemiological surveillance at bird ringing stations in Italy, and advocating further investigations on possible local transmission of <em>E. muris</em> in competent mammalian hosts.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102409"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Calf immunization protocols with low-virulence isolates of Anaplasma marginale: analysis of post-inoculation effects and protection against natural challenge\" [Ticks and Tick-borne Diseases 16 (2025) 102394].","authors":"Filipe Lucas de Melo Mendonça, Matheus Figueiredo Coelho, Camila Valgas Bastos, Júlia Angélica Gonçalves da Silveira, Rafael Romero Nicolino, Jose Azael Zambrano Uribe, Múcio Flávio Barbosa Ribeiro, Bruna Torres Silvestre, Rodrigo Melo Meneses, Antônio Último de Carvalho, Tiago Facury Moreira, Elias Jorge Facury-Filho","doi":"10.1016/j.ttbdis.2024.102412","DOIUrl":"10.1016/j.ttbdis.2024.102412","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":" ","pages":"102412"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and host factors underlying tick-borne virus infection in wild animals: Investigation of the emerging Yezo virus in Hokkaido, Japan","authors":"Mebuki Ito ,&nbsp;Miku Minamikawa ,&nbsp;Anastasiia Kovba ,&nbsp;Hideka Numata ,&nbsp;Tetsuji Itoh ,&nbsp;Takuma Ariizumi ,&nbsp;Asako Shigeno ,&nbsp;Yuki Katada ,&nbsp;Shiho Niwa ,&nbsp;Yurie Taya ,&nbsp;Yuto Shiraki ,&nbsp;Gita Sadaula Pandey ,&nbsp;Nariaki Nonaka ,&nbsp;Ryo Nakao ,&nbsp;Ryosuke Omori ,&nbsp;Yuma Ohari ,&nbsp;Norikazu Isoda ,&nbsp;Michito Shimozuru ,&nbsp;Toshio Tsubota ,&nbsp;Keita Matsuno ,&nbsp;Mariko Sashika","doi":"10.1016/j.ttbdis.2024.102419","DOIUrl":"10.1016/j.ttbdis.2024.102419","url":null,"abstract":"<div><div>Yezo virus (YEZV) is an emerging tick-borne virus that causes acute febrile illness. It has been continuously reported in patients and ticks in Japan and China since its first identification in Hokkaido, Japan. While serological tests have demonstrated that YEZV infections are prevalent in wild animals, such as raccoons (<em>Procyon lotor</em>), the determinants of infection in wild animals remain largely unknown. We examined the prevalence of YEZV in invasive raccoons, native tanukis (raccoon dogs, <em>Nyctereutes procyonoides albus</em>), and ticks in six study areas in Hokkaido between 2018 and 2023 to identify ecological factors underlying YEZV infection in wild animals. YEZV RNA fragments were detected in 0.22% of the 1,857 questing ticks. Anti-YEZV antibodies were detected in 32 of the 514 (6.2%) raccoon serum samples and in 5 of the 40 (12.5%) tanuki serum samples. Notably, the seroprevalence in raccoons varied significantly in one of the study areas over the years, that is, 0.0%, 60.0%, and 28.6% in 2021, 2022, and 2023, respectively, implying the temporary emergence of YEZV microfoci. By analyzing the tick load and YEZV seropositivity in raccoons in a field-based setting, we found a positive correlation between adult <em>Ixodes ovatus</em> load and YEZV-antibody positivity, highlighting the importance of <em>I. ovatus</em> in YEZV infection in wild animals. We also explored the environmental and host factors influencing YEZV seropositivity in raccoons and tanukis and found that landscape factors, such as the size of forest area around the trap site, were crucial for YEZV seropositivity in these animals. The significant variables for YEZV seropositivity in raccoons were partially different from those affecting tick infestation intensity in raccoons. The present results extend our understanding of tick-borne virus circulation in the field, emphasizing the unique ecology of the emerging YEZV.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102419"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular investigation of tick-borne pathogens from different regions of Morocco","authors":"Ana Cláudia Norte ,&nbsp;El-Mustapha Laghzaoui ,&nbsp;Andreia Guerreiro-Nunes ,&nbsp;El Hassan El Mouden ,&nbsp;Maria Sofia Núncio ,&nbsp;Rita de Sousa ,&nbsp;Isabel Lopes de Carvalho","doi":"10.1016/j.ttbdis.2024.102418","DOIUrl":"10.1016/j.ttbdis.2024.102418","url":null,"abstract":"<div><div>Tick-borne pathogens are a worldwide threat to public health that can only be mitigated by knowledge on tick-host associations coupled with surveillance of their infection by pathogenic microorganisms. This information is not equally available throughout tick vector distribution range and is deficient in some geographical areas. In this study we did a molecular survey of tick-borne pathogens associated with different tick species in Morocco. We analyzed four different species of <em>Hyalomma</em> (<em>Hyalomma aegyptium, Hyalomma anatolicum, Hyalomma dromedarii</em> and <em>Hyalomma impeltatum</em>) and <em>Rhipicephalus sanguineus</em> sensu lato ticks from four vertebrate hosts and from the vegetation. The most common tick species collected was <em>H. aegyptium</em> feeding on <em>Testudo graeca</em> tortoises, followed by <em>H. anatolicum</em> from the vegetation. <em>Hyalomma aegyptium</em> feeding on <em>T. graeca</em> was found infected with <em>Borrelia turcica</em>, representing the first detection for Western North Africa, and <em>Rickettsia sibirica</em> mongolitimonae, also detected for the first time in Morocco. <em>Rickettsia aeschlimannii</em> was also detected in <em>H. aegyptium</em> feeding on <em>T. graeca</em>. Additionally, <em>R. aeschlimannii</em> was detected in <em>H. anatolicum</em> from the vegetation, and <em>Rickettsia massiliae</em> in <em>R. sanguineus</em> s.l. from an Algerian hedgehog <em>Atelix algirus</em>. Because <em>H. aegyptium</em> are common parasites of <em>T. graeca</em> tortoises, and these animals are subjected to pet trade, it is important to evaluate the associated human health risks through regular surveillance and perform awareness campaigns for prevention of the general public.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102418"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks.","authors":"Anna Kloc, Angelina Wójcik-Fatla, Piotr Paprzycki, Lech Panasiuk","doi":"10.1016/j.ttbdis.2024.102421","DOIUrl":"10.1016/j.ttbdis.2024.102421","url":null,"abstract":"<p><p>Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens. In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts. DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp. In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic.</p>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"102421"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limited evidence of infection with other tick-borne pathogens in patients tested for Lyme neuroborreliosis in the Netherlands","authors":"Joris Koetsveld ,&nbsp;Alex Wagemakers ,&nbsp;Matthijs Brouwer ,&nbsp;Bob de Wever ,&nbsp;Ankje de Vries ,&nbsp;Steven van Gucht ,&nbsp;Anita Buskermolen ,&nbsp;Diederik van Beek ,&nbsp;Hein Sprong ,&nbsp;Joppe W. Hovius","doi":"10.1016/j.ttbdis.2024.102415","DOIUrl":"10.1016/j.ttbdis.2024.102415","url":null,"abstract":"<div><div><em>Ixodes ricinus</em> is the main vector of the causative agents of Lyme neuroborreliosis. This tick species can also transmit tick-borne encephalitis virus (TBEV), spotted fever group (SFG) <em>Rickettsia</em> and <em>Borrelia miyamotoi</em> to humans<em>.</em> These tick-borne pathogens are present in Dutch ticks and have also been associated with human neurological infections, but well characterized disease cases are seldom reported. We therefore assessed the evidence for TBEV, SFG <em>Rickettsia</em> or <em>B. miyamotoi</em> infection in clinically well-described patients suspected of Lyme neuroborreliosis.</div><div>We retrospectively included patients with specific predefined clinical criteria from patients that were tested for Lyme neuroborreliosis between 2010 and 2014 at an academic Lyme borreliosis Center. Serology was performed on available serum samples, and cerebrospinal fluid (CSF) was tested by molecular methods.</div><div>Out of 514 potentially eligible patients, 176 individual patients were included. None of CSF samples was positive for the tested tick-borne pathogens, except for one previously described patient with <em>Borrelia miyamotoi</em> disease (BMD). Serology revealed 27, 14 and three patients with antibodies against SFG <em>Rickettsia, B. miyamotoi</em> and TBEV, respectively. No distinctive clinical symptoms or signs could be associated with seropositivity against any of these tick-borne pathogens.</div><div>Apart from the previously published BMD case, we were unable to find convincing evidence of new cases of tick-borne encephalitis, spotted fever rickettsiosis or BMD in a cohort of patients suspected of Lyme neuroborreliosis. While antibodies against these tick-borne pathogens were detected, we could not associate these findings to clinical symptoms or signs. Therefore, prospective studies on humans with tick exposure are necessary to describe the prevalence, etiology and clinical symptoms of these tick-borne diseases other than Lyme borreliosis and tick-borne encephalitis.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102415"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of tick bites and associated care-seeking behaviors in the United States","authors":"Austin R. Earley ,&nbsp;Kiersten J. Kugeler ,&nbsp;Paul S. Mead ,&nbsp;Alison F. Hinckley","doi":"10.1016/j.ttbdis.2024.102416","DOIUrl":"10.1016/j.ttbdis.2024.102416","url":null,"abstract":"<div><div>Tick-borne diseases are commonly reported in the United States, but frequency of tick bites and care-seeking behaviors following tick bites are poorly understood. We used nationally representative survey data to describe the frequency of tick bites among people living in the United States and how often, where, and why care-seeking associated with tick bites occurs. We found that over 31 million people (nearly 1 in 10) living in the United States might experience a tick bite each year and highlight regional trends in associated care-seeking behaviors. These findings emphasize the need for effective tick bite prevention education and regionally tailored healthcare provider recommendations for management of tick-borne diseases.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102416"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New rules for the submission of review articles to Ticks and Tick-borne Diseases.","authors":"Olaf Kahl, Ben Mans, Ard Nijhof","doi":"10.1016/j.ttbdis.2024.102393","DOIUrl":"10.1016/j.ttbdis.2024.102393","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":" ","pages":"102393"},"PeriodicalIF":3.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}