Ticks and Tick-borne Diseases最新文献

{"title":"Ferritin-mediated regulation of gut microbiota homeostasis promotes blood-feeding adaptation in the tick Haemaphysalis doenitzi","authors":"Zhihua Gao ,&nbsp;Xiaofeng Xu ,&nbsp;Yanqing Shi ,&nbsp;Jiayi Zhang,&nbsp;Weijia Xing,&nbsp;Xinyu Zhang,&nbsp;Weikang Zhao,&nbsp;Ruya Cao,&nbsp;Xiaolong Yang","doi":"10.1016/j.ttbdis.2025.102554","DOIUrl":"10.1016/j.ttbdis.2025.102554","url":null,"abstract":"<div><div>As obligate hematophagous parasites, ticks have evolved to cope with substantial amounts of iron and exogenous microorganisms present in host blood during feeding. In ticks, ferritin plays an important role in maintaining the oxidative balance of gut and the homeostasis of the microbial community structure, but its regulatory mechanism has not yet been clarified. This study successfully identified a ferritin gene from <em>Haemaphysalis doenitzi</em>, named <em>Hd-fer</em>, and further studied the function of <em>Hd-fer</em>. The results showed that rHd-fer had antioxidant properties and antibacterial activity. The expression of <em>Hd-fer</em> gene in the ovary and midgut was significantly higher than other organs, and the expression in adults was significantly higher than other stages. The <em>Hd-fer</em> gene knock-out significantly changed the abundance of the midgut microbial community, and the relative abundance decreased generally, while the relative abundance of <em>Achromobacter</em> increased. The knockout of <em>Hd-fer</em> gene also significantly changed the structural composition of the midgut microbial species, and pathogenic microorganisms showed a growing trend, producing their unique microbial genera, including <em>Barnesiellaceae, Carnobacterium</em> and <em>Pediococcus</em>. The RNA interference of <em>Hd-fe</em>r led to prolonging the tick's blood sucking time and reducing engorged weight. The results of this study showed that Hd-fer may affect the stability of the midgut microbial community structure by regulating iron availability, which in turn plays an important role in the survival and reproduction of ticks. These findings provide novel insights into the role of ferritin in tick biology and highlight its potential as a target for controlling tick-borne diseases.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102554"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of equine piroplasmosis agents in Guadeloupe (Caribbeans): first report of Theileria haneyi, evaluation of diagnostic tools and impact of horse movement","authors":"Mickaël Mège ,&nbsp;Claire Bonsergent ,&nbsp;Laetitia Viry ,&nbsp;Mélanie Dhune ,&nbsp;Sylvie Lecollinet ,&nbsp;Laurence Malandrin","doi":"10.1016/j.ttbdis.2025.102547","DOIUrl":"10.1016/j.ttbdis.2025.102547","url":null,"abstract":"<div><div>Equine piroplasmosis is a major tick-borne horse disease, caused by the intracellular development of piroplasms (<em>Theileria equi</em> sensu lato and <em>Babesia caballi</em>), with significant economic and sanitary consequences. In 2024, 203 blood samples were collected in Guadeloupe (Caribbean) from asymptomatic horses. Using an 18S rRNA nested PCR (nPCR) specific for each equine genus parasite, 79 samples tested positive for <em>Theileria equi</em> and 9 for <em>Babesia caballi</em>, resulting in respective prevalence of 38.9% and 4.4%. Three horses were co-infected. For <em>B. caballi</em>, 18S rRNA sequence analysis revealed the presence of the genotype A only. For <em>T. equi,</em> the genotypes A and C were evidenced as mono-infections A (16/79, 20.3% of the infected horses) or mono-infections C (15/79, 19.0% of the infected horses). Interestingly, mono-infections with <em>T. equi</em> genotype E (17/79, 21.5% of the infected horses) were also detected, but only on horses imported from Europe and especially from metropolitan France, where this genotype is dominant. Further characterization using published <em>T. equi ema-1</em> and <em>T. haneyi</em> specific nPCRs revealed two major points. First, most 18S rRNA genotype C isolates (13/15) were detected using the <em>T. haneyi</em> specific nPCR. Second, the genotype E of <em>T. equi</em> could not be detected by any of these two nPCRs. Co-infection occurrence and types were then evaluated using a combination of the three analyses: 18S rRNA sequencing, <em>T. haneyi</em> specific nPCR and <em>T. equi ema-1</em> nPCR. Horses co-infected with the genotypes A and C (<em>T. haneyi</em>) represented the main population (32/79, 40.5% of the infected horses), while the co-infections AE (5/79, 6.3% of the horses) and CE (2/79, 2.5% of the horses) were rare. One horse was detected with a triple infection ACE. Taking into account all detected genotypes (120), 45.0% of the isolates belonged to the genotype A (54/120), 38.3% to the genotype C <em>T. haneyi</em> (46/120) and 16.7% to the Eurasian genotype E (20/120). The rarity of co-infections with the genotype E and the absence of this genotype on locally born horses suggest the absence of transmission of the genotype E by locally present vector ticks.</div><div>This work represents the first molecular record of <em>Theileria haneyi</em> in South and Central America and in the Caribbeans. We also demonstrate the introduction of <em>T. equi</em> genotype E from Europe with infected horses but not its installation, as well as a diagnostic issue to detect this genotype using PCR targeting <em>ema-1</em> gene.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102547"},"PeriodicalIF":3.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145160092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Various small mammal hosts are involved in the transmission cycle of zoonotic Anaplasma phagocytophilum in Southeastern Canada","authors":"Raphaëlle Audet-Legault ,&nbsp;Cécile Aenishaenslin ,&nbsp;Geneviève Baron ,&nbsp;Heather Coatsworth ,&nbsp;Marion Desmarchelier ,&nbsp;Vanessa Gabriele-Rivet ,&nbsp;Patrick A․ Leighton ,&nbsp;Jérôme Pelletier ,&nbsp;Jean-Philippe Rocheleau ,&nbsp;Catherine Bouchard","doi":"10.1016/j.ttbdis.2025.102551","DOIUrl":"10.1016/j.ttbdis.2025.102551","url":null,"abstract":"<div><div>Human granulocytic anaplasmosis (HGA) is a tick-borne disease emerging in North America, caused by the zoonotic variant Ap-ha (human active) of the bacterium <em>Anaplasma phagocytophilum.</em> This research aimed to understand the reservoir competency of small mammals for Ap-ha in the province of Québec, Canada. To achieve this aim, wild small mammals were sampled from June to August in 2022 and 2023 and tested for Ap and its variants (Ap-ha, Ap-v1) using qPCR targeting the <em>msp2</em> and 16S genes. In total, 547 small mammals from nine different taxa were live captured across two years<em>.</em> Nearly half (48.2 %) of small mammals carried at least one <em>Ixodes scapularis</em> tick larva and 29.3 % carried at least one <em>I. scapularis</em> nymph. The odds of Ap infection were three times higher in eastern chipmunks (OR = 2.98, <em>p</em> &lt; 0.05) and five times lower in red squirrels (OR = 0.21, <em>p</em> &lt; 0.01) when compared to <em>Peromyscus</em> spp. mice. When infected, <em>Peromyscus</em> mice and eastern chipmunks transmitted the infection on average to 6.8 % (CI95: 24.9–48.7) and 52.9 % (CI95: 16.7–89.1) of feeding larvae, respectively. An Ap variant could be identified in 90 % (109/121) of infected small mammals, all of which were the Ap-ha strain. In conclusion, eastern chipmunks and <em>Peromyscus</em> mice should be considered as reservoir hosts contributing to the transmission and emergence of anaplasmosis in Canada. This study also confirms the circulation of the Ap-ha variant within host and tick communities in Southeastern Canada.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102551"},"PeriodicalIF":3.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145160093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing disease surveillance in rhinoceroses: A multiplex real-time PCR assay for detecting Theileria bicornis and Babesia bicornis","authors":"Naledi P. Sekgobela ,&nbsp;Ilse Vorster ,&nbsp;Milana Troskie ,&nbsp;Melvyn Quan ,&nbsp;David E. Zimmerman ,&nbsp;Ayesha Hassim ,&nbsp;Luis Neves ,&nbsp;Raksha V. Bhoora","doi":"10.1016/j.ttbdis.2025.102552","DOIUrl":"10.1016/j.ttbdis.2025.102552","url":null,"abstract":"<div><div>Black (<em>Diceros bicornis</em>) and white (<em>Ceratotherium simum</em>) rhinoceroses in Southern Africa face multiple threats, including poaching, habitat loss, and translocation stress. Infections with <em>Theileria bicornis</em> (in both rhino species) and <em>Babesia bicornis</em> (confirmed only in black rhinos) add further health risks, with stressors such as translocation potentially increasing susceptibility. Effective management requires sensitive molecular diagnostic assays for accurate detection and surveillance. To address this, we developed a multiplex qPCR assay (MqTbBb) using species-specific TaqMan™ minor groove binder (MGB) probes for the simultaneous detection of <em>T. bicornis</em> and <em>B. bicornis</em>. The assay targets 18S rRNA gene regions, amplifying an 87 bp fragment for <em>T. bicornis</em> and a 51 bp fragment for <em>B. bicornis</em>, with efficiencies of 100 % and 98 %, respectively. Probit analysis determined a 95 % Limit of detection of 1.00 × 10⁻⁶ % and 6.27 × 10⁻⁶ % equivalent parasitized erythrocytes for <em>T. bicornis</em> and <em>B. bicornis</em>, respectively. No cross-reactivity was observed with other related protozoa tested. A total of 223 field samples from rhinos (101 black and 122 white) in Mpumalanga province were screened using both the MqTbBb and Reverse Line Blot (RLB) hybridization assays. The MqTbBb detected <em>T. bicornis</em> in 57 % of black and 99 % of white rhinos, with co-infections in 40 % of black rhinos. RLB detected <em>T. bicornis</em> in 96 % of black and 95 % of white rhinos, with a <em>Babesia</em> catch-all probe signal in 75 % and 32 %, respectively. <em>B. bicornis</em> was not detected by RLB and was never detected as a single infection by qPCR. These findings highlight high <em>T. bicornis</em> prevalence and rare <em>B. bicornis</em> infections (co-infections). The MqTbBb assay strengthens detection, surveillance, and conservation efforts.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102552"},"PeriodicalIF":3.4,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression patterns of chemokine mediators and TLR10 in natural bovine babesiosis and anaplasmosis infections","authors":"Mayne Barboza Sarti ,&nbsp;Geovana Menegão de Souza ,&nbsp;Camila Fagionato Agostinho ,&nbsp;Gustavo Henrique Carvalho Borges ,&nbsp;Flávia Fernanda Carneiro Santana ,&nbsp;Luciana Morita Katiki ,&nbsp;Anibal Eugênio Vercesi Filho ,&nbsp;Ana Gonçalves Domingos ,&nbsp;Rodrigo Giglioti","doi":"10.1016/j.ttbdis.2025.102546","DOIUrl":"10.1016/j.ttbdis.2025.102546","url":null,"abstract":"<div><div>Cattle tick fever, caused by the protozoa <em>Babesia bovis</em> and <em>B. bigemina</em> and the rickettsial bacterium <em>Anaplasma marginale</em>, represents a significant constraint to livestock productivity worldwide. Taurine cattle are considered more susceptible to these hemoparasites than zebuine breeds. Chemokines and Toll-like Receptors (TLRs) play key roles in immune processes such as chemotaxis and inflammation, primarily through interactions with respective receptors. This study assessed the relationship between the expression of four chemokine-related genes (<em>ccr3, cxcl12, cxcl8,</em> and <em>cxcr1</em>) and one TLR (<em>tlr10</em>) with the infection levels of <em>B. bovis, B. bigemina</em>, and <em>A. marginale</em> in 24 naturally infected calves from two genetic groups (Angus [<em>n</em> = 13; 100% taurine] and Ultrablack [<em>n</em> = 11; 82% Angus, 18% zebuine]). Blood samples were collected every 30 days over six time points (Nov 2021–Apr 2022). DNA was used to quantify hemoparasite loads by qPCR (log DNA copy number, CNlog), while RNA was used for RT-qPCR-based gene expression analysis. A mixed model was used to assess associations between gene expression and infection levels, controlling for evaluation, sex, genetic group, and their interactions. All genes, except <em>cxcl12</em>, showed significant associations with at least one hemoparasite. Higher <em>B. bovis</em> and <em>B. bigemina</em> CNlog values were linked to reduced expression of most genes, whereas <em>A. marginale</em> infection was associated with increased gene expression. These findings highlight distinct immune modulation strategies by hemoparasites and underscore the need for species-specific interventions. Further studies are required to explore the mechanisms by which these mediators influence susceptibility or resistance in cattle.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102546"},"PeriodicalIF":3.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assortative mating in Ixodes scapularis Say, 1821 (Acari: Ixodidae)","authors":"Lorenza Beati ,&nbsp;Matteo Montagna ,&nbsp;Jean Tsao ,&nbsp;Thierry de Meeûs","doi":"10.1016/j.ttbdis.2025.102550","DOIUrl":"10.1016/j.ttbdis.2025.102550","url":null,"abstract":"<div><div>Mate choice influences levels of inbreeding and genetic diversity in natural populations. Positive assortative mating (PAM), for instance, has been described in <em>Ixodes ricinus</em> L., but this behavior has yet to be investigated in the closely related <em>Ixodes scapularis</em> Say, 1821<em>.</em> By analyzing nine microsatellite loci of 30 females and 30 males removed <em>in copula</em> from hunter-harvested deer specimens at the Savannah River Site (South Carolina) during the hunting season of fall 2010, we found a weak but significant assortative pairing signature in a population that, otherwise, appeared genetically homogeneous. This indicated that in spite of a low average relative relatedness between members of the same pair, the difference between observed and all other possible male-female pairs was highly significant. The results suggested that our non-coding microsatellite loci were drafted by genes coding for PAM, through genetic hitchhiking. To explain our data, such genes would need to be homogeneously distributed in the genome and span from a few genes with major effects on PAM to many genes with moderate or weak effects on PAM.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102550"},"PeriodicalIF":3.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the number of Lyme neuroborreliosis cases in European Centre for Disease Prevention and Control (ECDC) data and national public surveillance reports, 2018–2023","authors":"Alexander Davidson ,&nbsp;Frederick J. Angulo ,&nbsp;Julie Davis ,&nbsp;Kate Halsby ,&nbsp;Gordon Brestrich ,&nbsp;Jennifer C. Moïsi ,&nbsp;James H. Stark","doi":"10.1016/j.ttbdis.2025.102542","DOIUrl":"10.1016/j.ttbdis.2025.102542","url":null,"abstract":"<div><div>Lyme borreliosis (LB) is the most common vector-borne disease in Europe. At least 29 European Economic Area (EEA) countries publish online LB surveillance reports. However, countries have different criteria for what is notifiable for LB surveillance, limiting comparability. EEA countries began reporting Lyme neuroborreliosis (LNB) cases, a disseminated LB manifestation, to the European Centre for Disease Prevention and Control (ECDC) in 2018, using a uniform case definition. This analysis compared LNB data from ECDC and national surveillance reports to evaluate their utility in monitoring LB in Europe. LNB surveillance data from 2018 to 2023 were downloaded from ECDC. Nationally reported online LB surveillance data from EEA countries were identified and reviewed. Countries that reported LNB cases in national surveillance and to ECDC were included and case data were compared. Of 31 EEA countries during 2018 to 2023, 11 (35 %) differentiated LNB cases in their national published LB surveillance; eight of these also reported to ECDC. The number of LNB cases published in national reports in these eight countries did not exactly match the number of LNB cases reported to ECDC but overall disease trends were similar. Comparing the LNB cases from eight countries in ECDC and national surveillance demonstrates that both capture evolving LNB disease trends despite some differences in the number of cases reported to each. To allow for more robust comparisons of LB burden and trends across Europe, harmonizing case definitions and reporting mechanisms for LB would provide great value.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 6","pages":"Article 102542"},"PeriodicalIF":3.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145108878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rickettsial exposure in ruminants from Somalia","authors":"Aamir M. Osman ,&nbsp;Igor S. Silito ,&nbsp;Ahmed A. Hassan-Kadle ,&nbsp;Mohamed A. Shair ,&nbsp;Abdalla M. Ibrahim ,&nbsp;Maria Carolina A. Serpa ,&nbsp;Thállitha S.W.J. Vieira ,&nbsp;Marcelo B. Labruna ,&nbsp;Rafael F.C. Vieira","doi":"10.1016/j.ttbdis.2025.102543","DOIUrl":"10.1016/j.ttbdis.2025.102543","url":null,"abstract":"<div><div><em>Rickettsia</em> spp. are Gram-negative obligate intracellular bacteria, with <em>Rickettsia africae</em> being transmitted by <em>Amblyomma</em> ticks and posing a zoonotic risk. The status of diseases like rickettsiosis is largely unknown in Somalia. Our study investigates rickettsial exposure in livestock across two different regions in the country. A cross-sectional study collected 372 (190 goats, 133 cattle, 49 sheep) serum samples from the Benadir and Lower Shabelle regions of Somalia. Indirect immunofluorescence assays (IFA) were used to detect anti-<em>R. africae</em> and anti-<em>Rickettsia rhipicephali</em> antibodies, with sera diluted in two-fold increments starting at 1:64. Out of 372 samples, 188/372 (50.5 %) (endpoint titer: 64–2048) were seropositive for rickettsial antigens, with <em>R. africae</em> alone detected in 78/372 (21.0 %) and <em>R. rhipicephali</em> alone in 38/372 (10.2 %). Co-reactivity to both rickettsial antigens occurred in 72/372 (19.4 %) of samples. Cattle showed the highest seroreactivity at 90.2 %, mainly for <em>R. africae</em>, followed by sheep at 28.6 %, and goats at 28.4 %. Cattle and sheep were more likely to be seropositive than goats (OR: 24.5 and 1.1, respectively). This study provides the first serological evidence of <em>Rickettsia</em> spp. in ruminants from Somalia. Our findings suggest heightened susceptibility in cattle, posing health risks to humans, especially if cattle are considered sentinels for human exposure to <em>R. africae.</em></div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102543"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An assessment of the possible recent establishment of Hyalomma rufipes in Hungary","authors":"Gergő Keve ,&nbsp;Tekla Varga ,&nbsp;Márton Hoitsy ,&nbsp;Kata Wermer ,&nbsp;Sándor Hornok","doi":"10.1016/j.ttbdis.2025.102544","DOIUrl":"10.1016/j.ttbdis.2025.102544","url":null,"abstract":"<div><div>The aim of this study was to assess the viability of an opportunistic population of <em>Hyalomma rufipes</em>, as evidence of reproduction had been documented in the southern part of Central Europe, specifically Hungary, in 2022. To assess the current situation, tick collections targeting various mammalian species were organized with the assistance of local veterinarians between September 2022 and May 2024. Over the study period, 1502 ticks were collected; however, none belonged to the <em>Hyalomma</em> genus. <em>Dermacentor reticulatus</em> dominated in our sample population (<em>n</em> = 963), followed by <em>Ixodes ricinus</em> (<em>n</em> = 436). Other species found in the area were: <em>Haemaphysalis concinna</em> (<em>n</em> = 45)<em>, Dermacentor marginatus</em> (<em>n</em> = 38)<em>, Ixodes hexagonus</em> (<em>n</em> = 13), <em>Alloceraea inermis</em> (<em>n</em> = 6), and <em>Rhipicephalus sanguineus</em> s.s. (<em>n</em> = 1). Two <em>Hyalomma marginatum</em> males were received from veterinarians between 2022 and 2024, although these were collected far from our study area. Our results suggests that the <em>H. rufipes</em> population recorded in 2022 did not persist.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102544"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual increase in tularemia incidence in Alsace, Northeastern France in 2024","authors":"M. Martinot ,&nbsp;T. Lemmet ,&nbsp;A. Barthel ,&nbsp;P. Suntharasarma ,&nbsp;M. Mohseni-Zadeh ,&nbsp;G. Gregorowicz ,&nbsp;P. Boyer ,&nbsp;Y. Hansmann ,&nbsp;V. Gerber","doi":"10.1016/j.ttbdis.2025.102545","DOIUrl":"10.1016/j.ttbdis.2025.102545","url":null,"abstract":"<div><div>Tularemia is a bacterial zoonosis endemic to the Northern Hemisphere, transmitted through various modes and presenting with diverse clinical manifestations. In recent years, the number of notifications (tularemia is a reportable disease in France) have increased and the spectrum of clinical forms have changed. We conducted a retrospective study of tularemia incidence diagnosed in 2024 at two major hospitals in Alsace, a small region of Northeastern France. This study included 10 cases (7 men and 3 women; mean age = 52 years) reported in 2024. Clinical forms comprised six ulceroglandular or glandular cases, two oropharyngeal forms, and two pleuropulmonary forms including a fatal case in a 73-year-old immunocompromised patient.. Three patients reported recent tick bites, while two others had frequent tick exposure. In 9 of the 10 cases, tularemia was not initially suspected by primary care physicians, leading to delayed diagnosis, inappropriate treatment, and complications. Nationwide, 150 cases were reported in 2024, including 33 from the Northeastern region, of which 10 were our cases. Compared to other regions of France, cases in Alsace showed a higher proportion associated with tick-related transmission. Our findings reveal a peak in tularemia incidence in Alsace in 2024. Primary care physicians often failed to recognize tularemia, resulting in delayed treatment and complications—even in cases with typical glandular forms. Tularemia is an increasing zoonosis that may be under recognized among general practitioners. There is a need for increased awareness and education among healthcare providers and the public.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102545"},"PeriodicalIF":3.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}