Prevalence of tick-borne pathogens in feeding and questing Ixodes ricinus ticks from Southern Sweden

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES

Ticks and Tick-borne Diseases Pub Date : 2025-02-12 DOI:10.1016/j.ttbdis.2025.102453

Chiara Cialini , Alessandra Cafiso , Mattias Waldeck , Åsa Lundgren , Johan Fält , Bo Settergren , Phimphanit Choklikitumnuey , Giulia Chiappa , Eleonora Rosso , Laura Roveri , Elisa Fesce , Nicola Ferrari , Per-Eric Lindgren , Chiara Bazzocchi , Giulio Grandi

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引用次数: 0

Abstract

Ixodes ricinus, the most common tick species in Northern Europe, plays a significant role as a vector of several pathogens, with its geographical distribution expanding in recent years. In Southern Sweden, particularly in Region Skåne County (referred to as Skåne), the favorable climate and landscape conditions support extensive proliferation of I. ricinus. Despite Lyme borreliosis being common in this region and few annual cases of tick-borne encephalitis (TBE) being reported, data on the circulation of tick-borne pathogens (TBPs) remain limited. This study molecularly investigated the presence of Anaplasma phagocytophilum, Babesia spp., Borrelia spp., and TBE virus (TBEV) in I. ricinus ticks (n = 1000). In detail, questing ticks (82 adults and 196 nymphs) were collected from vegetation in forest and meadow areas, while 581, 80 and 8 feeding adults were collected from 39 roe deer, 6 fallow deer and 1 moose, respectively. Additionally, 53 feeding adults were removed from domestic animals (42 from four dogs and 11 from one cat).

The molecular analyses detected Anaplasma phagocytophilum, Borrelia spp., and Babesia spp. in 54 %, 24 %, 3.2 % of host-feeding ticks and in 0.40 %, 35 %, 3.6 % of questing ticks, respectively. In detail, for Borrelia and Babesia genera, the following species were detected: Borrelia miyamotoi, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi s.s., Babesia microti and Babesia venatorum. TBEV was not detected.

Moreover, the relationship between the feeding duration of the roe deer-collected ticks and their PCR-positivity for Borrelia spp. and A. phagocytophilum was also modeled. The results showed a reduction in the probability of tick infection with Borrelia spp. as attachment time increased, supporting evidence that roe deer serum exerts a borreliacidal effect. This study highlights the presence of several zoonotic TBPs in Skåne, emphasizing the need for a structured monitoring plan and preventive strategies within a One Health framework.

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.