Ticks and Tick-borne Diseases最新文献

{"title":"Posterior reversible encephalopathy syndrome in early lyme neuroborreliosis","authors":"Beate Hagenkötter ,&nbsp;Souheil Zayet ,&nbsp;Samantha Poloni ,&nbsp;Vincent Gendrin ,&nbsp;Marie Zanusso ,&nbsp;Silviu Stancescu ,&nbsp;Timothée Klopfenstein","doi":"10.1016/j.ttbdis.2024.102383","DOIUrl":"10.1016/j.ttbdis.2024.102383","url":null,"abstract":"<div><p>We report a case of posterior reversible encephalopathy syndrome in an adult patient fulfilling criteria for proven early Lyme neuroborreliosis.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102383"},"PeriodicalIF":3.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000761/pdfft?md5=d2013604f73df495b68e9fd48a94d8be&pid=1-s2.0-S1877959X24000761-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Alpha-Gal glycolipids in saliva of Lone-Star Tick (Amblyomma americanum)","authors":"Surendra Raj Sharma ,&nbsp;Sabir Hussain ,&nbsp;Shailesh K. Choudhary ,&nbsp;Scott P. Commins ,&nbsp;Shahid Karim","doi":"10.1016/j.ttbdis.2024.102384","DOIUrl":"10.1016/j.ttbdis.2024.102384","url":null,"abstract":"<div><p>Alpha-Gal Syndrome (AGS) is a delayed allergic reaction triggered by IgE antibodies targeting galactose-α-1,3-galactose (α-gal), prevalent in red meat. Its global significance has increased, with over 450,000 estimated cases in the United States alone. AGS is linked to tick bites, causing sensitization and elevated α-gal specific IgE levels. However, the precise mechanisms and tick intrinsic factors contributing to AGS development post-tick bites remain unclear. This study aims to characterize the alpha-gal conjugated lipid antigens in <em>Amblyomma americanum</em> (<em>Am. americanum</em>) salivary glands and saliva. Nanospray ionization mass spectrometry (NSI-MS) analysis revealed the identification of α-gal bound lipid antigens in <em>Am. americanum</em> saliva. Additionally, the activation of basophils by extracted alpha-gal bound lipids and proteins provides evidence of their antigenic capabilities.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102384"},"PeriodicalIF":3.1,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000773/pdfft?md5=c9b760211bf8b8ecfdca8c7b46099cef&pid=1-s2.0-S1877959X24000773-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141762056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-omics approach for understanding blood digestion dynamics in Ixodes scapularis and identification of anti-tick vaccine targets","authors":"Jeremiah B. Reyes ,&nbsp;Molly McVicar ,&nbsp;Saransh Beniwal ,&nbsp;Arvind Sharma ,&nbsp;Richard Tillett ,&nbsp;Juli Petereit ,&nbsp;Andrew Nuss ,&nbsp;Monika Gulia-Nuss","doi":"10.1016/j.ttbdis.2024.102379","DOIUrl":"10.1016/j.ttbdis.2024.102379","url":null,"abstract":"<div><p><em>Ixodes scapularis,</em> the black-legged tick<em>,</em> is a major arthropod vector that transmits the causative agents of Lyme disease and several other pathogens of human significance. The tick midgut is the main tissue involved in blood acquisition and digestion and the first organ to have contact with pathogens ingested through the blood meal. Gene expression in the midgut before, during, and after a blood meal may vary in response to the physiological changes due to blood feeding. A systems biology approach based on RNA and protein sequencing was used to gain insight into the changes in tick midgut transcripts and proteins during blood ingestion (unfed and partially fed) and digestion (1-, 2-, 7-, and 14 days post detachment from the host) by the <em>Ixodes scapularis</em> female ticks. A total of 2,726 differentially expressed transcripts, and 449 proteins were identified across the time points. Genes involved in detoxification of xenobiotics, proteases, protease inhibitors, metabolism, and immunity were differentially expressed in response to blood feeding. Similarly, proteins corresponding to the same groups were also differentially expressed. Nine genes from major gene categories were chosen as potential vaccine candidates, and, using RNA interference, the effect of these gene knockdowns on tick biology was investigated. Knockdown of these genes had variable negative impacts on tick physiology, such as the inability to engorge fully and to produce eggs and increased mortality. These and additional gene targets provide opportunities to explore novel tick control strategies.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102379"},"PeriodicalIF":3.1,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000724/pdfft?md5=b3d85f71955aebabbb5fad285334949f&pid=1-s2.0-S1877959X24000724-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First data on cholesterol metabolism in Ornithodoros argasid ticks: Molecular and functional characterization of the N-terminal domain of Niemann-Pick C1 proteins","authors":"Lucía de Dios-Blázquez ,&nbsp;Ana Laura Cano-Argüelles ,&nbsp;Ricardo Pérez-Sánchez ,&nbsp;María González-Sánchez ,&nbsp;Ana Oleaga","doi":"10.1016/j.ttbdis.2024.102382","DOIUrl":"10.1016/j.ttbdis.2024.102382","url":null,"abstract":"<div><p>Cholesterol is a molecule vital for tick physiology, but ticks cannot synthesize it and rely on dietary cholesterol. Therefore, tick proteins involved in cholesterol absorption and transport, such as the Niemann-Pick type C1 domain-containing (NPC1) proteins, are promising targets for anti-tick vaccine development. The aim of this study was to assess the structure, function, and protective efficacy of the NPC1 orthologues identified previously in the midgut transcriptomes of argasid ticks <em>Ornithodoros erraticus</em> and <em>Ornithodoros moubata</em>. For this purpose, their corresponding cDNA coding sequences were cloned and sequenced, their secondary and 3D structures were predicted, and their function was evaluated through RNAi-mediated gene knockdown and <em>in vitro</em> feeding on blood supplemented with ezetimibe, which inhibits cholesterol binding by NPC1 proteins. Subsequently, the protective efficacy of a recombinant form of NPC1 from <em>O. moubata</em> (rOmNPC1) was tested in a rabbit vaccine trial. While inhibiting cholesterol absorption with ezetimibe resulted in up to 77 % mortality in adult <em>O. moubata</em>, NPC1 gene knockdown and vaccination with rOmNPC1 decreased female reproductive performance in terms of the number and fertility of laid eggs. This study presents the initial molecular and functional insights into NPC1 proteins in soft ticks and supports the hypothesis that disrupting cholesterol metabolism diminishes tick viability and reproduction, rendering Niemann-Pick type C1 domain-containing proteins promising targets for drugs or vaccines.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102382"},"PeriodicalIF":3.1,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X2400075X/pdfft?md5=a4433a0e0c8368d094ad095e5be25fbc&pid=1-s2.0-S1877959X2400075X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and phylogenetic analysis of Nairobi sheep disease virus from Haemaphysalis longicornis ticks in Shandong Province, China","authors":"Yunxiao Wang ,&nbsp;Ruiling Zhang ,&nbsp;Xiurong Wang ,&nbsp;Xudong Zhang ,&nbsp;Zhong Zhang ,&nbsp;Michael J. Carr ,&nbsp;Guangfu Yu ,&nbsp;Hong Zhou ,&nbsp;Weifeng Shi","doi":"10.1016/j.ttbdis.2024.102375","DOIUrl":"10.1016/j.ttbdis.2024.102375","url":null,"abstract":"<div><p>Nairobi Sheep Disease (NSD) is a typical tick-borne syndrome characterized by severe hemorrhagic gastroenteritis, spontaneous abortion, and a high case fatality rate in small ruminants. The pathogenic agent, Nairobi sheep disease virus (NSDV), has also been associated with human infections, indicating its possible zoonotic potential. Prior to this study, NSDV has been detected from ticks collected in Jilin, Hubei, and Liaoning provinces in China. In the present study, a total of 343 ticks (<em>Haemaphysalis longicornis</em>) were collected in Shandong province, China in 2020, and pooled into 16 libraries. Analysis of the meta-transcriptomic sequencing data identified NSDV strains SDWL07, SDWL08, and SDWL16 from three pools. The SDWL07 and SDWL16 strains were detected from unfed ticks, while SDWL08 was detected from cattle-feeding ticks. Phylogenetic analyses showed higher sequence identities between the three strains and other Chinese NSDV strains than those from India and Kenya. Phylogenetic analyses also revealed that they clustered together and fell within the China lineage, suggesting no potential genetic reassortment among them. In summary, this is the first report of the identification of NSDV in Shandong province, highlighting the continually expanding endemic regions of this pathogen. Surveillance of NSDV should be intensified in China, especially in areas where <em>H. longicornis</em> is endemic.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102375"},"PeriodicalIF":3.1,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000682/pdfft?md5=f5482294951c0b792f501a69c0ecfec3&pid=1-s2.0-S1877959X24000682-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ticks parasitizing cold-blooded animals from three different Brazilian biomes; with note for males of Amblyomma rotundatum","authors":"Hermes Ribeiro Luz ,&nbsp;Carlos Eduardo Costa de Campos ,&nbsp;Livio Martins Costa-Junior ,&nbsp;Ercileide Silva Santos ,&nbsp;Adriani Hass ,&nbsp;Dauana Mesquita Sousa ,&nbsp;Ruth Myrian de Moares e Silva ,&nbsp;Josiane Moreira Rocha ,&nbsp;George Rego Albuquerque ,&nbsp;Antonio Jorge Argolo ,&nbsp;Rogério Zacariotti ,&nbsp;Catia Dejuste de Paula ,&nbsp;Luiz Cezar Machado Pereira ,&nbsp;Patricia Avello Nicola ,&nbsp;João Luiz Horacio Faccini ,&nbsp;Mauricio Claudio Horta ,&nbsp;Rafael Michael Silva Nogueira ,&nbsp;Leonardo Teixeira DallAgnol ,&nbsp;Marcelo Bahia Labruna ,&nbsp;Thiago Fernandes Martins","doi":"10.1016/j.ttbdis.2024.102377","DOIUrl":"10.1016/j.ttbdis.2024.102377","url":null,"abstract":"<div><p>Ticks parasitize a wide variety of wild animals, including amphibians and reptiles. In addition to the possibility of microorganism transmission to these hosts, ticks can also cause severe bleeding, and high parasitism can lead to death. Therefore, knowing the diversity of ticks parasitizing amphibians and reptiles is important for conservation and preservation measures for these vertebrates. In the present study, we report parasitism by ticks in amphibians and reptiles from different Brazilian biomes (Amazon, Caatinga, Cerrado and Atlantic Forest). Ticks were collected from amphibians and reptiles deposited from the Herpetological Collection of the Federal University of Maranhão (UFMA), São Luís (Maranhão State), the State University Santa Cruz (UESC), Ilhéus (Bahia State), and the Federal University of São Francisco Valley (Univasf), Petrolina (Pernambuco State). Additionally, ticks were collected from amphibians and reptiles captured and road-killed in the Amazon biome, at Maranhão and Amapá States. Specimens of ticks were photographed under a Zeiss stereomicroscope (5.1 zoom). Map with the locations were made using the Qgis program. Overall, 1973 specimens of amphibians and reptiles were examined. A total of 927 ticks were collected: 98 larvae, 421 nymphs and 408 adults. Six species of ticks were identified: <em>Amblyomma rotundatum</em> and <em>Amblyomma dissimile</em> the most frequent, and <em>Amblyomma cajennense</em> sensu stricto, <em>Amblyomma sculptum, Amblyomma nodosum</em> and <em>Amblyomma humerale</em>, occasionally. Surprisingly, a total of twelve males of <em>A. rotundatum</em> were collected. Here we report new records of association between cold-blooded animals and ticks and reinforce the absence of <em>A. dissimile</em> in the Caatinga, Cerrado and Atlantic Forest biomes. Additionally, we report new records of <em>A. rotundatum</em> males on reptiles in the Amazon biome. This last record allows us to speculate about a possible association of <em>A. rotundatum</em> males with reptiles and the Amazon biome.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102377"},"PeriodicalIF":3.1,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000700/pdfft?md5=ec6dc23da8ff74c866ac9ca63f64487a&pid=1-s2.0-S1877959X24000700-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wide distribution of Beiji nairoviruses and related viruses in Ixodes ticks in Japan","authors":"Mai Kishimoto ,&nbsp;Yukari Itakura ,&nbsp;Koshiro Tabata ,&nbsp;Rika Komagome ,&nbsp;Hiroki Yamaguchi ,&nbsp;Kohei Ogasawara ,&nbsp;Ryo Nakao ,&nbsp;Yongjin Qiu ,&nbsp;Kozue Sato ,&nbsp;Hiroki Kawabata ,&nbsp;Masahiro Kajihara ,&nbsp;Naota Monma ,&nbsp;Junji Seto ,&nbsp;Asako Shigeno ,&nbsp;Masayuki Horie ,&nbsp;Michihito Sasaki ,&nbsp;William W. Hall ,&nbsp;Hirofumi Sawa ,&nbsp;Yasuko Orba ,&nbsp;Keita Matsuno","doi":"10.1016/j.ttbdis.2024.102380","DOIUrl":"10.1016/j.ttbdis.2024.102380","url":null,"abstract":"<div><p>Beiji nairovirus (BJNV), in the family <em>Nairoviridae</em>, the order <em>Bunyavirales</em>, was recently reported as a causative agent of an emerging tick-borne zoonotic infection in China. This study investigated the prevalence of BJNV in ticks in Japan. Screening of over 2,000 ticks from multiple regions revealed a widespread distribution of BJNV and BJNV-related viruses in Japan, particularly in the northern island, and in other high altitude areas with exclusive occurrence of <em>Ixodes</em> ticks. Phylogenetic analysis identified three distinct groups of nairoviruses in ticks in Japan: BJNV, Yichun nairovirus (YCNV) and a newly identified Mikuni nairovirus (MKNV). BJNV and YCNV variants identified in ticks in Japan exhibited high nucleotide sequence identities to those in China and Russia with evidence of non-monophyletic evolution among BJNVs, suggesting multiple cross-border transmission events of BJNV between the Eurasian continent and Japan. Whole genome sequencing of BJNV and MKNV revealed a unique GA-rich region in the S segment, the significance of which remains to be determined. In conclusion, the present study has shown a wide distribution and diversity of BJNV-related nairoviruses in <em>Ixodes</em> ticks in Japan and has identified unique genomic structures. The findings demonstrate the significance of BJNV as well as related viruses in Japan and highlight the necessity of monitoring emerging nairovirus infections and their potential risks to public health.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102380"},"PeriodicalIF":3.1,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000736/pdfft?md5=4e7ba51c65498aa470bcda45c4c01065&pid=1-s2.0-S1877959X24000736-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141602032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"A transwell assay method to evaluate Borrelia burgdorferi sensu stricto migratory chemoattraction toward tick saliva proteins\" [Ticks Tick Borne Dis. 2021 Sep; 12(5): 101782. PMCID: PMC10895706].","authors":"Taylor J Van Gundy, Amy J Ullmann, Kevin S Brandt, Robert D Gilmore","doi":"10.1016/j.ttbdis.2024.102378","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102378","url":null,"abstract":"","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":" ","pages":"102378"},"PeriodicalIF":3.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular survey of Rickettsia spp. in ticks infesting wild animals in six departments in Colombia","authors":"Johnathan Alvarez‑Londoño ,&nbsp;Daniel Felipe Giraldo-Noreña ,&nbsp;Estefani T. Martínez-Sánchez ,&nbsp;Fredy A. Rivera‑Páez ,&nbsp;Nubia E. Matta","doi":"10.1016/j.ttbdis.2024.102381","DOIUrl":"10.1016/j.ttbdis.2024.102381","url":null,"abstract":"<div><p>Ticks are a globally distributed group of hematophagous ectoparasites that parasitize terrestrial vertebrates such as amphibians, reptiles, birds, and mammals. Ticks are vectors and reservoirs of pathogens that play an important role in wildlife and human health. <em>Rickettsia</em> is one of the bacteria transmitted by ticks, which some pathogenic species can cause rickettsiosis, a zoonotic disease that can cause serious harm to humans and animals. More information is necessary on the interactions between ticks and wildlife despite the fifty-seven ticks species already identified in Colombia. The objective of the present study was to determine the associations between ticks parasitizing wildlife and bacteria of the genus <em>Rickettsia</em> in six departments of Colombia. One hundred eighty-five ticks (80 larvae, 78 nymphs, and 27 adults) were collected from 55 wildlife species (amphibians, birds, mammals, and reptiles). Nine tick species were identified, and <em>Rickettsia bellii, Rickettsia felis</em>, ‘<em>Candidatus</em> Rickettsia colombianensi’ and <em>Rickettsia parkeri</em> were detected. Our results contribute to the current knowledge of tick-associated rickettsiae and the role of wildlife in their transmission dynamics.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102381"},"PeriodicalIF":3.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000748/pdfft?md5=e74705b9d240ba9e128b36a73cca348f&pid=1-s2.0-S1877959X24000748-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemaphysalis longicornis (Acari: Ixodidae) does not transmit Babesia bovis, a causative agent of cattle fever","authors":"Karen C. Poh ,&nbsp;Mitzi Aguilar ,&nbsp;Janaína Capelli-Peixoto ,&nbsp;Sara K. Davis ,&nbsp;Massaro W. Ueti","doi":"10.1016/j.ttbdis.2024.102374","DOIUrl":"https://doi.org/10.1016/j.ttbdis.2024.102374","url":null,"abstract":"<div><p>The Asian longhorned tick (<em>Haemaphysalis longicornis</em>) was first reported in the United States in 2017 and has since been detected in at least 17 states. This tick infests cattle and can produce large populations quickly due to its parthenogenetic nature, leading to significant livestock mortalities and economic losses. While <em>H. longicornis</em> has not been detected in Texas, species distribution models have identified southern Texas as a possible hospitable region for this tick. Southern Texas is currently home to the southern cattle tick (<em>Rhipicephalus microplus</em>), which can transmit the causative agent of cattle fever (<em>Babesia bovis</em>). With the potential for <em>H. longicornis</em> and <em>B. bovis</em> to overlap in southern Texas and their potential to negatively impact the national and global livestock industry, it is imperative to identify the role <em>H. longicornis</em> may play in the cattle fever disease system. A controlled acquisition and transmission experiment tested whether <em>H. longicornis</em> is a vector for <em>B. bovis</em>, with the <em>R. microplus</em>-<em>B. bovis</em> system used as a positive control. Transstadial (nymphs to adults) and transovarial (adults to larvae) transmission and subsequent transstadial maintenance (nymphs and adults) routes were tested in this study. Acquisition-fed, splenectomized animals were used to increase the probability of tick infection. Acquisition nymphs were macerated whole and acquisition adults were dissected to remove midguts and ovaries at five time points (4, 6, 8, 10, and 12 days post-repletion), with 40 ticks processed per time point and life stage. The greatest percentage of nymphs with detectable <em>B. bovis</em> DNA occurred six days post-repletion (20.0 %). For adults, the percentage of positive midguts and ovaries increased as days post-repletion progressed, with day 12 having the highest percentage of positive samples (67.5 % and 60.0 %, respectively). When egg batches were tested in triplicate, all <em>H. longicornis</em> egg batches were negative for <em>B. bovis</em>, while all <em>R. microplus</em> egg batches were positive for <em>B. bovis</em>. During the transmission phase, the subsequent life stages for transstadial (adults) and transovarial transmission/transstadial maintenance (larvae, nymphs, and adults) were fed on naïve, splenectomized calves. All life stages of <em>H. longicornis</em> ticks tested during transmission were negative for <em>B. bovis</em>. Furthermore, the transmission fed animals were also negative for <em>B. bovis</em> and did not show signs of bovine babesiosis during the 45-day post tick transmission period. Given the lack of successful transstadial or transovarial transmission, it is unlikely that <em>H. longicornis</em> is a vector for <em>B. bovis</em>.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 6","pages":"Article 102374"},"PeriodicalIF":3.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X24000670/pdfft?md5=215732cf088db90a4fffbaf4317cbeaa&pid=1-s2.0-S1877959X24000670-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}