Biomolecular NMR Assignments最新文献_第9页

NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b 成纤维细胞生长因子8剪接异构体b的核磁共振分配

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-04-29 DOI: 10.1007/s12104-023-10132-8

Bruno Hargittay, Konstantin S. Mineev, Christian Richter, Sridhar Sreeramulu, Hendrik R.A. Jonker, Krishna Saxena, Harald Schwalbe

引用次数: 0

Resonance assignments of the microtubule-binding domain of the microtubule-associated protein 7 (MAP7) 微管相关蛋白7 (MAP7)微管结合域的共振配位

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-04-26 DOI: 10.1007/s12104-023-10124-8

Agnes Adler, Lenette F. Kjaer, J. Wouter Beugelink, Marc Baldus, Hugo van Ingen

引用次数: 1

Chemical shift assignments of calmodulin bound to a cytosolic domain of GluN2A (residues 1004–1024) from the NMDA receptor NMDA受体结合GluN2A胞质结构域(残基1004-1024)的钙调蛋白的化学移位配位

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-04-07 DOI: 10.1007/s12104-023-10125-7

Aritra Bej, James B. Ames

引用次数: 0

1H, 15N, 13C backbone and Cβ resonance assignments for UBQLN1 UBA and UBAA domains UBQLN1 UBA和UBAA结构域的1H, 15N, 13C骨架和Cβ共振分配

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-04-06 DOI: 10.1007/s12104-023-10127-5

Gwen R. Buel, Xiang Chen, Olumide Kayode, Anthony Cruz, Kylie J. Walters

{"title":"1H, 15N, 13C backbone and Cβ resonance assignments for UBQLN1 UBA and UBAA domains","authors":"Gwen R. Buel, Xiang Chen, Olumide Kayode, Anthony Cruz, Kylie J. Walters","doi":"10.1007/s12104-023-10127-5","DOIUrl":"10.1007/s12104-023-10127-5","url":null,"abstract":"<div><p>UBQLN1 functions in autophagy and proteasome-mediated protein degradation. It contains an N-terminal ubiquitin-like domain (UBL), a C-terminal ubiquitin-associated domain (UBA), and a flexible central region which functions as a chaperone to prevent protein aggregation. Here, we report the <sup>1</sup>H, <sup>15</sup>N, and <sup>13</sup>C resonance assignments for the backbone (<sup>N</sup>H, N, C’, Cα, and Hα) and sidechain Cβ atoms of the UBQLN1 UBA and an N-terminally adjacent segment called the UBA-adjacent domain (UBAA). We find a subset of the resonances corresponding to the UBAA to have concentration-dependent chemical shifts, likely due to self-association. We also find the backbone amide nitrogen of T572 to be shifted upfield relative to the average value for a threonine amide nitrogen, a phenomenon likely caused by T572 Hγ1 engagement in a hydrogen bond with adjacent backbone carbonyl atoms. The assignments described in this manuscript can be used to study the protein dynamics of the UBQLN1 UBA and UBAA as well as the interaction of these domains with other proteins.</p></div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"17 1","pages":"101 - 106"},"PeriodicalIF":0.9,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12104-023-10127-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4238043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1H, 15N, and 13C chemical shift backbone resonance NMR assignment of the accumulation-associated protein (Aap) lectin domain from Staphylococcus epidermidis 表皮葡萄球菌积累相关蛋白(Aap)凝集素结构域的1H, 15N和13C化学位移骨干核磁共振分配

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-04-06 DOI: 10.1007/s12104-023-10126-6

Rahul Yadav, Tanveer Shaikh, Suhas Tikole, Andrew B. Herr, Nicholas C. Fitzkee

引用次数: 0

Backbone NMR assignment of the yeast expressed Fab fragment of the NISTmAb reference antibody 对表达NISTmAb参比抗体Fab片段的酵母进行骨架核磁共振鉴定

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-03-01 DOI: 10.1007/s12104-023-10123-9

Tsega L. Solomon, Kinlin Chao, Genevieve Gingras, Yves Aubin, William B. O’Dell, John P. Marino, Robert G. Brinson

引用次数: 2

Backbone and side chain chemical shift assignment of diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris, an organophosphorus-degrading enzyme 有机磷降解酶Loligo vulgaris二异丙基氟磷酸酶(DFPase)的主链和侧链化学移位配位

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-02-10 DOI: 10.1007/s12104-023-10120-y

Julian C.-H. Chen, Marco Tonelli, Penelope Anderson, Ryszard Michalczyk, Marc-Michael Blum, Robert F. Williams

引用次数: 0

1H, 13C, 15N Backbone and sidechain chemical shift assignments of the C-terminal domain of human UDP-glucuronosyltransferase 2B17 (UGT2B17-C) 人udp -葡萄糖醛酸基转移酶2B17 (UGT2B17-C) c端结构域的1H, 13C, 15N主链和侧链化学位移定位

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-02-09 DOI: 10.1007/s12104-023-10122-w

Anamika Sulekha, Michael J. Osborne, Jadwiga Gasiorek, Katherine L. B. Borden

{"title":"1H, 13C, 15N Backbone and sidechain chemical shift assignments of the C-terminal domain of human UDP-glucuronosyltransferase 2B17 (UGT2B17-C)","authors":"Anamika Sulekha, Michael J. Osborne, Jadwiga Gasiorek, Katherine L. B. Borden","doi":"10.1007/s12104-023-10122-w","DOIUrl":"10.1007/s12104-023-10122-w","url":null,"abstract":"<div><p>UDP-glucuronosyltransferases are the principal enzymes involved in the glucuronidation of metabolites and xenobiotics for physiological clearance in humans. Though glucuronidation is an indispensable process in the phase II metabolic pathway, UGT-mediated glucuronidation of most prescribed drugs (> 55%) and clinical evidence of UGT-associated drug resistance are major concerns for therapeutic development. While UGTs are highly conserved enzymes, they manifest unique substrate and inhibitor specificity which is poorly understood given the dearth of experimentally determined full-length structures. Such information is important not only to conceptualize their specificity but is central to the design of inhibitors specific to a given UGT in order to avoid toxicity associated with pan-UGT inhibitors. Here, we provide the <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N backbone (~ 90%) and sidechain (~ 62%) assignments for the C-terminal domain of UGT2B17, which can be used to determine the molecular binding sites of inhibitor and substrate, and to understand the atomic basis for inhibitor selectivity between UGT2B17 and other members of the UGT2B subfamily. Given the physiological relevance of UGT2B17 in the elimination of hormone-based cancer drugs, these assignments will contribute towards dissecting the structural basis for substrate specificity, selective inhibitor recognition and other aspects of enzyme activity with the goal of selectively overcoming glucuronidation-based drug resistance.</p></div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"17 1","pages":"67 - 73"},"PeriodicalIF":0.9,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4382835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Backbone NMR resonance assignment of the apo human Tsg101-UEV domain 载子人Tsg101-UEV结构域的主核磁共振分配

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-02-06 DOI: 10.1007/s12104-023-10119-5

Danai Moschidi, François-Xavier Cantrelle, Emmanuelle Boll, Xavier Hanoulle

{"title":"Backbone NMR resonance assignment of the apo human Tsg101-UEV domain","authors":"Danai Moschidi, François-Xavier Cantrelle, Emmanuelle Boll, Xavier Hanoulle","doi":"10.1007/s12104-023-10119-5","DOIUrl":"10.1007/s12104-023-10119-5","url":null,"abstract":"<div><p>The Endosomal Sorting Complex Required for Transport (ESCRT) pathway, through inverse topology membrane remodeling, is involved in many biological functions, such as ubiquitinated membrane receptor trafficking and degradation, multivesicular bodies (MVB) formation and cytokinesis. Dysfunctions in ESCRT pathway have been associated to several human pathologies, such as cancers and neurodegenerative diseases. The ESCRT machinery is also hijacked by many enveloped viruses to bud away from the plasma membrane of infected cells. Human tumor susceptibility gene 101 (Tsg101) protein is an important ESCRT-I complex component. The structure of the N-terminal ubiquitin E2 variant (UEV) domain of Tsg101 (Tsg101-UEV) comprises an ubiquitin binding pocket next to a late domain [P(S/T)AP] binding groove. These two binding sites have been shown to be involved both in the physiological roles of ESCRT-I and in the release of the viral particles, and thus are attractive targets for antivirals. The structure of the Tsg101-UEV domain has been characterized, using X-ray crystallography or NMR spectroscopy, either in its apo-state or bound to ubiquitin or late domains. In this study, we report the backbone NMR resonance assignments, including the proline signals, of the apo human Tsg101-UEV domain, that so far was not publicly available. These data, that are in good agreement with the crystallographic structure of Tsg101-UEV domain, can therefore be used for further NMR studies, including protein-protein interaction studies and drug discovery.</p></div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"17 1","pages":"49 - 54"},"PeriodicalIF":0.9,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4246206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical shift assignments of calmodulin bound to the GluN1 C0 domain (residues 841–865) of the NMDA receptor NMDA受体glun1c0结构域(残基841-865)上钙调蛋白的化学移位配位

IF 0.9 4区生物学

Biomolecular NMR Assignments Pub Date : 2023-02-05 DOI: 10.1007/s12104-023-10121-x

Aritra Bej, James B. Ames

引用次数: 2