Progress in Molecular Biology and Translational Science最新文献_第3页

Visualizing and mapping cell death: Biochemical tools, techniques and assays. 可视化和绘制细胞死亡：生化工具、技术和分析。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-07-05 DOI: 10.1016/bs.pmbts.2025.06.018

Sadhna Soni, Ashwini Nath Tiwari, Amrendra Singh, Arun Upadhyay

{"title":"Visualizing and mapping cell death: Biochemical tools, techniques and assays.","authors":"Sadhna Soni, Ashwini Nath Tiwari, Amrendra Singh, Arun Upadhyay","doi":"10.1016/bs.pmbts.2025.06.018","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2025.06.018","url":null,"abstract":"Cell death is a fundamental process that plays a role in the development of multicellular organisms, tissue homeostasis, and fighting infections. Dysfunctional cell death signaling is associated with many diseases, including cancer. Most studied among multiple possible cell death pathways is apoptosis that is essential for various biological functions, including embryogenesis, aging, and the development of numerous diseases. This regulated cell death enables the removal of cells in a controlled manner, maintaining tissue homeostasis and aiding organismal development. Understanding cell death pathways can help develop new therapeutic strategies for treating diseases like cancer. For example, tumor cells often avoid apoptosis, which can lead to treatment resistance. Various groups of researchers believe that studying other cell death pathways, like necroptosis, pyroptosis, ferroptosis, and cuproptosis, may have high potential for cancer therapy. Numerous biochemical methods exist to detect, quantify, and analyze cell death pathways, each with unique principles, advantages, and limitations. A comprehensive understanding of these methods enables researchers to select appropriate techniques for their experimental contexts. This chapter systematically discusses the molecular and cellular changes related to various cell death pathways and the conventional and non-conventional methods used to investigate these processes.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"217 ","pages":"81-107"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 前言。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 DOI: 10.1016/S1877-1173(25)00027-4

Tanveer A Dar, Laishram R Singh, Vladimir N Uversky

引用次数: 0

COVID-19 related complications. COVID-19相关并发症。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-03-06 DOI: 10.1016/bs.pmbts.2025.02.002

Muhamed Adilović

引用次数: 0

Neutrophils and COVID-19. 中性粒细胞与COVID-19。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-03-05 DOI: 10.1016/bs.pmbts.2025.02.003

Jasmin Šutković

{"title":"Neutrophils and COVID-19.","authors":"Jasmin Šutković","doi":"10.1016/bs.pmbts.2025.02.003","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2025.02.003","url":null,"abstract":"Neutrophils are the first line of defense against pathogens, most effectively by forming Neutrophil Extracellular Traps (NETs). Neutrophiles are further classified into several subpopulations during their development, eliminating pathogens through various mechanisms. However, due to the chaotic and uncontrolled immune response, NETs are often severely resulting in tissue damage and lung infections. The uncontrolled and poorly acknowledged host response regarding the cytokine storm is one of the major causes of severe COVID-19 conditions. Specifically, the increased formation of low-density neutrophils (LDNs), together with neutrophil extracellular traps (NETs) is closely linked with the severity and poor prognosis in patients with COVID-19. In this review, we discuss in detail the ontogeny of neutrophils at different stages and their recruitment and activation after infections, focusing on SARS-CoV-2. In addition, this chapter summarized the research progress on potential targeted drugs (NETs and Cytokine inhibitors) for neutrophil medical therapy and hoped to provide reference for the development of related therapeutic drugs for critically ill COVID-19 patients.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"213 ","pages":"347-384"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current approaches in CRISPR-Cas system for metabolic disorder. CRISPR-Cas系统治疗代谢紊乱的最新方法。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2024-08-16 DOI: 10.1016/bs.pmbts.2024.07.016

Yajushii Arora, Priya, Manishankar Kumar, Dhruv Kumar

{"title":"Current approaches in CRISPR-Cas system for metabolic disorder.","authors":"Yajushii Arora, Priya, Manishankar Kumar, Dhruv Kumar","doi":"10.1016/bs.pmbts.2024.07.016","DOIUrl":"10.1016/bs.pmbts.2024.07.016","url":null,"abstract":"A new era in genomic medicine has been brought by the development of CRISPR-Cas technology, which presents hitherto unheard-of possibilities for the treatment of metabolic illnesses. The treatment approaches used in CRISPR/Cas9-mediated gene therapy, emphasize distribution techniques such as viral vectors and their use in preclinical models of metabolic diseases like hypercholesterolemia, glycogen storage diseases, and phenylketonuria. The relevance of high-throughput CRISPR screens for target identification in discovering new genes and pathways associated with metabolic dysfunctions is an important aspect of the discovery of new approaches. With cutting-edge options for genetic correction and cellular regeneration, the combination of CRISPR-Cas technology with stem cell therapy has opened new avenues for the treatment of metabolic illnesses. The integration of stem cell therapy and CRISPR-Cas technology is an important advance in the treatment of metabolic diseases, which are difficult to treat because of their intricate genetic foundations. This chapter addresses the most recent developments in the application of stem cell therapy and CRISPR-Cas systems to treat a variety of metabolic disorders, providing fresh hope for effective and maybe curative therapies. This chapter examines techniques and developments that have been made recently to address a variety of metabolic disorders using CRISPR-Cas systems. Our chapter focuses on the foundational workings of CRISPR-Cas technology and its potential uses in gene editing, gene knockout, and activation/repression-based gene modification.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"210 ","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in CRISPR/Cas9 system for eye disorders. CRISPR/Cas9系统治疗眼部疾病的最新进展。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2024-08-17 DOI: 10.1016/bs.pmbts.2024.07.018

D A Ayush Gowda, Girish Birappa, Sripriya Rajkumar, C Bindu Ajaykumar, Bhavana Srikanth, Sammy L Kim, Vijai Singh, Aparna Jayachandran, Junwon Lee, Suresh Ramakrishna

{"title":"Recent progress in CRISPR/Cas9 system for eye disorders.","authors":"D A Ayush Gowda, Girish Birappa, Sripriya Rajkumar, C Bindu Ajaykumar, Bhavana Srikanth, Sammy L Kim, Vijai Singh, Aparna Jayachandran, Junwon Lee, Suresh Ramakrishna","doi":"10.1016/bs.pmbts.2024.07.018","DOIUrl":"10.1016/bs.pmbts.2024.07.018","url":null,"abstract":"Ocular disorders encompass a broad spectrum of phenotypic and clinical symptoms resulting from several genetic variants and environmental factors. The unique anatomy and physiology of the eye facilitate validation of cutting-edge gene editing treatments. Genome editing developments have allowed researchers to treat a variety of diseases, including ocular disorders. The clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system holds considerable promise for therapeutic applications in the field of ophthalmology, including repair of aberrant genes and treatment of retinal illnesses related to the genome or epigenome. Application of CRISPR/Cas9 systems to the study of ocular disease and visual sciences have yielded innovations including correction of harmful mutations in patient-derived cells and gene modifications in several mammalian models of eye development and disease. In this study, we discuss the generation of several ocular disease models in mammalian cell lines and in vivo systems using a CRISPR/Cas9 system. We also provide an overview of current uses of CRISPR/Cas9 technologies for the treatment of ocular pathologies, as well as future challenges.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"210 ","pages":"21-46"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell death mechanisms during Mycobacterium tuberculosis infection: A perspective from a host-pathogen interface. 结核分枝杆菌感染期间的细胞死亡机制：从宿主-病原体界面的视角。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-07-08 DOI: 10.1016/bs.pmbts.2025.06.020

Prachi Nangpal, Nupur Angrish, Garima Khare

{"title":"Cell death mechanisms during Mycobacterium tuberculosis infection: A perspective from a host-pathogen interface.","authors":"Prachi Nangpal, Nupur Angrish, Garima Khare","doi":"10.1016/bs.pmbts.2025.06.020","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2025.06.020","url":null,"abstract":"Tuberculosis, caused by Mycobacterium tuberculosis (Mtb), is a life threatening disease, which accounts for millions of lives annually. Mtb is an intracellular bacterium, has coevolved with humans to premeditate its machinery to surpass the immunity mounted against it in order to persist for long durations in the system. Cell death is a fundamental process required not only for tissue homeostasis but also for providing protection against intracellular pathogens. Various forms of cell death processes are known including apoptosis, pyroptosis, necroptosis, autophagy etc., that have been shown to play important roles in anti-TB immunity against Mtb. Moreover, inhibition of these pathways by Mtb is considered as one of the virulence mechanisms by which the pathogen is able to survive and replicate inside the host. Apart from identification of newer drug targets and development of anti-TB drugs that solely target the pathogen, recent advancements have been made in developing host-directed therapies against TB, which are aimed at modulating the host responses to reduce excessive inflammation and tissue damage. Thus, understanding the proteins and signaling cascades associated with cell death modalities and their relation with Mtb infection will give us new insights into the area of host-pathogen interactions and help us design better host-directed therapies.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"217 ","pages":"355-406"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current findings and potential applications of cell death research in ovarian cancer. 卵巢癌细胞死亡研究的最新发现及潜在应用

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-07-05 DOI: 10.1016/bs.pmbts.2025.06.019

Anh-Dao Ngo, Tung-Duong Luu, Hai-Quang Pham, Ha-Le Dang, Dinh-Toi Chu

{"title":"Current findings and potential applications of cell death research in ovarian cancer.","authors":"Anh-Dao Ngo, Tung-Duong Luu, Hai-Quang Pham, Ha-Le Dang, Dinh-Toi Chu","doi":"10.1016/bs.pmbts.2025.06.019","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2025.06.019","url":null,"abstract":"One of the top gynecological health problems for women is ovarian cancer (OC) and it causes a huge disease burden not only for women but for all of humanity. The disease's immense burden comes from it often detected at advanced stages, current treatments such as chemotherapy or radiotherapy are not very effective, leading to a low 5-year survival rate. Therefore, discovering new therapeutic approaches that target the processes contributing to ovarian tumor formation and metastasis is urgently needed. Among them, regulating cell death processes is emerging as a promising therapy for eliminating cancer including ovarian cancer. Many scientists have been researching and clinically testing methods and drug molecules to control cell death processes consisting of apoptosis, necroptosis, ferroptosis, cuproptosis, or pyroptosis to destroy tumors. In this work, we displayed an overview about the importance of cell death in the control of ovarian carcinoma cells. According to the current knowledge of the mechanisms of cell death, targeted drugs and approaches have been studied to modulate the activation/inhibition of this process in the treatment of ovarian tumor. Thanks to that, new hopes are explored to help improve the effectiveness of cancer diagnosis and treatment.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"217 ","pages":"197-209"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 前言。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 DOI: 10.1016/S1877-1173(25)00011-0

Vijai Singh

引用次数: 0

Molecular crowding and amyloidogenic self-assembly: Emergent perspectives from modern computations. 分子拥挤和淀粉样蛋白自组装：从现代计算的新兴观点。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2025-01-01 Epub Date: 2025-01-21 DOI: 10.1016/bs.pmbts.2024.10.001

Hindol Chatterjee, Neelanjana Sengupta

{"title":"Molecular crowding and amyloidogenic self-assembly: Emergent perspectives from modern computations.","authors":"Hindol Chatterjee, Neelanjana Sengupta","doi":"10.1016/bs.pmbts.2024.10.001","DOIUrl":"10.1016/bs.pmbts.2024.10.001","url":null,"abstract":"In recent decades, the conventional protein folding paradigm has been challenged by intriguing properties of disordered peptide sequences that do not adopt stably folded conformations. Such intrinsically disordered proteins and protein regions (IDPs and IDRs) are poised uniquely in biology due to their propensity for self-aggregation, amyloidogenesis, and correlations with a cluster of debilitating diseases. Complexities underlying their structural and functional manifestations are enhanced in the presence of molecular crowding via non-specific protein-protein and protein-solvent contacts. Enabled by technological advances, physics-based algorithms, and data science, modern computer simulations provide unprecedented insights into the structure, function, dynamics, and thermodynamics of complex macromolecular systems. These characteristics are frequently correlated and manifest into unique observables. This chapter presents an overview of how such methodologies can lend insights and drive investigations into the molecular trifecta of crowding, protein self-aggregation, and amyloidogenesis. It begins with a general overview of disordered proteins in relation to biological function and of a suite of relevant experimental methods. Specific examples are showcased in the biological context. This is followed by a description of the computational approaches that supplant experimental efforts, with an elaboration on enhanced molecular simulation methods. The chapter concludes by alluding to expanded possibilities in disease amelioration.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"211 ","pages":"209-247"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0