Biometals最新文献

{"title":"Publisher Correction: Global threat posed by metals and metalloids in the changing environment: a One Health approach to mechanisms of toxicity","authors":"Wing-Kee Lee, Frank Thévenod, Elmar J. Prenner","doi":"10.1007/s10534-024-00617-x","DOIUrl":"10.1007/s10534-024-00617-x","url":null,"abstract":"","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1305 - 1305"},"PeriodicalIF":4.1,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel tetraaza macrocyclic Schiff base complexes of bivalent zinc: microwave-assisted green synthesis, spectroscopic characterization, density functional theory calculations, molecular docking studies, in vitro antimicrobial and anticancer activities","authors":"Mamta,&nbsp;Ashu Chaudhary","doi":"10.1007/s10534-024-00616-y","DOIUrl":"10.1007/s10534-024-00616-y","url":null,"abstract":"<div><p>In the present manuscript, novel macrocyclic Schiff base complexes [Zn(N₄MacL₁)Cl₂–Zn(N₄MacL₃)Cl₂] were synthesized by the reaction of ZnCl₂ and macrocyclic ligands (N₄MacL₁–N₄MacL₃) derived from diketone and diamines under microwave irradiation method and conventional method. The structures of the obtained complexes were identified by various spectrometric methods such as Fourier transformation infra-red (FT-IR), nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HR-MS), powder X-ray diffraction, molar conductivity, and UV–vis. The structures of the synthesized compounds were optimized by using the def2–TZV/J and def2–SVP/J Coulomb fitting basis sets at B3LYP level in density functional theory (DFT) calculations. The macrocyclic Schiff base complexes exhibited higher activities against Gram-positive bacteria (<i>Staphylococcus aureus</i> and <i>Bacillus cereus</i>), Gram-negative bacteria (<i>Escherichia coli</i> and <i>Xanthomonas campestris</i>), and fungal strains (<i>Fusarium oxysporum</i> and <i>Candida albicans</i>) in comparison to macrocyclic Schiff base ligands. Furthermore, the newly synthesized macrocyclic compounds were assessed for their anticancer activity against three cell lines: A549 (human alveolar adenocarcinoma epithelial cell line), HT-29 (human colorectal adenocarcinoma cell line), and MCF-7 (human breast adenocarcinoma cell line) using the MTT assay. The obtained results showed that the macrocyclic complex [Zn(N₄MacL₃)Cl₂] displayed the highest cytotoxic activity (2.23 ± 0.25 µM, 6.53 ± 0.28 µM, and 7.40 ± 0.45 µM for A549, HT-29, and MCF-7 cancer cell lines, respectively). Additionally, molecular docking investigations were conducted to elucidate potential molecular interactions between the synthesized macrocyclic compounds and target proteins. The results revealed a consistent agreement between the docking calculations and the experimental data.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 6","pages":"1431 - 1456"},"PeriodicalIF":4.1,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Functional characterization of SLC39 family members ZIP5 and ZIP10 in overexpressing HEK293 cells reveals selective copper transport activity","authors":"Marcello Polesel,&nbsp;Alvaro Ingles-Prieto,&nbsp;Eirini Christodoulaki,&nbsp;Evandro Ferrada,&nbsp;Cédric Doucerain,&nbsp;Patrick Altermatt,&nbsp;Michelle Knecht,&nbsp;Michael Kuhn,&nbsp;Anna-Lena Steck,&nbsp;Maria Wilhelm,&nbsp;Vania Manolova","doi":"10.1007/s10534-024-00615-z","DOIUrl":"10.1007/s10534-024-00615-z","url":null,"abstract":"","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 4","pages":"1037 - 1038"},"PeriodicalIF":4.1,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11254955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141441911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the antifungal mechanisms of CTP, a new copper(II)-theophylline/1,10-phenanthroline complex, on drug-resistant non-albicans Candida species","authors":"Heloisa F. Frota,&nbsp;Pedro F. Barbosa,&nbsp;Carolline M. A. Lorentino,&nbsp;Lorena R. F. Affonso,&nbsp;Lívia S. Ramos,&nbsp;Simone S. C. Oliveira,&nbsp;Lucieri O. P. Souza,&nbsp;Olufunso O. Abosede,&nbsp;Adeniyi S. Ogunlaja,&nbsp;Marta H. Branquinha,&nbsp;André L. S. Santos","doi":"10.1007/s10534-024-00605-1","DOIUrl":"10.1007/s10534-024-00605-1","url":null,"abstract":"<div><p><i>Candida</i> species undeniably rank as the most prevalent opportunistic human fungal pathogens worldwide, with <i>Candida albicans</i> as the predominant representative. However, the emergence of non-<i>albicans Candida</i> species (NACs) has marked a significant shift, accompanied by rising incidence rates and concerning trends of antifungal resistance. The search for new strategies to combat antifungal-resistant <i>Candida</i> strains is of paramount importance. Recently, our research group reported the anti-<i>Candida</i> activity of a coordination compound containing copper(II) complexed with theophylline (theo) and 1,10-phenanthroline (phen), known as “CTP” – Cu(theo)₂phen(H₂O).5H₂O. In the present work, we investigated the mechanisms of action of CTP against six medically relevant, antifungal-resistant NACs, including <i>C. auris</i>, <i>C. glabrata</i>, <i>C. haemulonii</i>, <i>C. krusei</i>, <i>C. parapsilosis</i> and <i>C. tropicalis</i>. CTP demonstrated significant efficacy in inhibiting mitochondrial dehydrogenases, leading to heightened intracellular reactive oxygen species production. CTP treatment resulted in substantial damage to the plasma membrane, as evidenced by the passive incorporation of propidium iodide, and induced DNA fragmentation as revealed by the TUNEL assay. Scanning electron microscopy images of post-CTP treatment NACs further illustrated profound alterations in the fungal surface morphology, including invaginations, cavitations and lysis. These surface modifications significantly impacted the ability of <i>Candida</i> cells to adhere to a polystyrene surface and to form robust biofilm structures. Moreover, CTP was effective in disassembling mature biofilms formed by these NACs. In conclusion, CTP represents a promising avenue for the development of novel antifungals with innovative mechanisms of action against clinically relevant NACs that are resistant to antifungals commonly used in clinical settings.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1237 - 1253"},"PeriodicalIF":4.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of ferroptosis in DM-induced liver injury","authors":"Keping Wu,&nbsp;Jiasi Chen,&nbsp;Jiawen Lin,&nbsp;Enyi Zhu,&nbsp;Xiaochang Xu,&nbsp;Xiuhong Yan,&nbsp;Lang Ju,&nbsp;Mingcheng Huang,&nbsp;Yimin Zhang","doi":"10.1007/s10534-024-00600-6","DOIUrl":"10.1007/s10534-024-00600-6","url":null,"abstract":"<div><p>The liver damage caused by Diabetes Mellitus (DM) has attracted increasing attention in recent years. Liver injury in DM can be caused by ferroptosis, a form of cell death caused by iron overload. However, the role of iron transporters in this context is still not clear. Herein, we attempted to shed light on the pathophysiological mechanism of ferroptosis. DM was induced in 8-week-old male rats by streptozotocin (STZ) before assessment of the degree of liver injury. Together with histopathological changes, variations in glutathione peroxidase 4 (GPX4), glutathione (GSH), superoxide dismutase (SOD), transferrin receptor 1 (TFR1), ferritin heavy chain (FTH), ferritin light chain (FTL), ferroportin and Prussian blue staining, were monitored in rat livers before and after treatment with Fer-1. In the liver of STZ-treated rats, GSH and SOD levels decreased, whereas those of malondialdehyde (MDA) increased. Expression of TFR1, FTH and FTL increased whereas that of glutathione peroxidase 4 (GPX4) and ferroportin did not change significantly. Prussian blue staining showed that iron levels increased. Histopathology showed liver fibrosis and decreased glycogen content. Fer-1 treatment reduced iron and MDA levels but GSH and SOD levels were unchanged. Expression of FTH and FTL was reduced whereas that of ferroportin showed a mild decrease. Fer-1 treatment alleviated liver fibrosis, increased glycogen content and mildly improved liver function. Our study demonstrates that ferroptosis is involved in DM-induced liver injury. Regulating the levels of iron transporters may become a new therapeutic strategy in ferroptosis-induced liver injury.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1191 - 1200"},"PeriodicalIF":4.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of biologically active cefpodoxime and vanillin-based schiff base metal complexes with the detailed biological evaluations","authors":"Naeem Razaq,&nbsp;Amina Asghar,&nbsp;Amna Mumtaz,&nbsp;Samiah H. Al-Mijalli,&nbsp;Mehr un Nisa,&nbsp;Tauheeda Riaz,&nbsp;Munawar Iqbal,&nbsp;Bilal Shahid","doi":"10.1007/s10534-024-00601-5","DOIUrl":"10.1007/s10534-024-00601-5","url":null,"abstract":"<div><p>Schiff bases of existing antimicrobial drugs are an area, which is still to be comprehensively explored to improve drug efficiency against consistently resisting bacterial species. In this study, we have targeted a new and eco-friendly method of condensation reaction that allows the \"green synthesis\" as well as improved biological efficacy. The transition metal complexes of cefpodoxime with well-enhanced biological activities were synthesized. The condensation reaction product of cefpodoxime and vanillin was further reacted with suitable metal salts of [Mn (II), Cu (II), Fe (II), Zn (II), and Ni (II)] with 1:2 molar ratio (metal: ligand). The characterization of all the products were carried out by using UV–Visible, elemental analyzer, FTIR, ¹H-NMR, ICP-OES, and LC–MS. Electronic data obtained by UV–Visible proved the octahedral geometry of metal complexes. The biological activities Schiff base ligand and its transition metal complexes were tested by using <i>in-vitro</i> anti-bacterial analysis against various Gram-negative, as well as Gram-positive bacterial strains. Proteinase and protein denaturation inhibition assays were utilized to evaluate the products <i>in-vitro</i> anti-inflammatory activities. The in vitro antioxidant activity of the ligand and its complexes was evaluated by utilizing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) <i>in-vitro</i> method. The final results proved metal complexes to be more effective against bacterial microorganisms as compared to respective parent drug as well as their free ligands. <i>Patch Dock</i>, a molecular docking tool, was used to dock complexes 1a-5e with the crystal structure of GlcN-6-P synthase (ID: 1MOQ). According to the docking results, complex <b>2b</b> exhibited a highest score (8,882; ACE = –580.43 kcal/mol) that is well correlated with a high inhibition as compared to other complexes which corresponds to the antibacterial screening outcomes.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1201 - 1224"},"PeriodicalIF":4.1,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arsenite tolerance and removal potential of the indigenous halophilic bacterium, Halomonas elongata SEK2","authors":"Nazanin Tavoosi,&nbsp;Abbas Akhavan Sepahi,&nbsp;Vahid Kiarostami,&nbsp;Mohammad Ali Amoozegar","doi":"10.1007/s10534-024-00612-2","DOIUrl":"10.1007/s10534-024-00612-2","url":null,"abstract":"<div><p>The indigenous halophilic arsenite-resistant bacterium <i>Halomonas elongata</i> strain SEK2 isolated from the high saline soil of Malek Mohammad hole, Lut Desert, Iran, could tolerate high concentrations of arsenate (As⁵⁺) and arsenite (As³⁺) up to 800 and 40 mM in the SW-10 agar medium, respectively. The isolated strain was able to tolerate considerable concentrations of other toxic heavy metals and oxyanions, including Cadmium (Cd²⁺), Chromate (Cr⁶⁺), lead (Pb²⁺), and selenite (Se⁴⁺), regarding the high salinity of the culture media (with a total salt concentration of 10% (w/v)), the tolerance potential of the isolate SEK2 was unprecedented. The bioremoval potential of the isolate SEK2 was examined through the Silver diethyldithiocarbamate (SDDC) method and demonstrated that the strain SEK2 could remove 60% of arsenite from arsenite-containing growth medium after 48 h of incubation without converting it to arsenate. The arsenite adsorption or uptake by the halophilic bacterium was investigated and substantiated through Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), and Energy Dispersive X-ray (EDX) analyses. Furthermore, Transmission electron microscope (TEM) analysis revealed ultra-structural alterations in the presence of arsenite that could be attributed to intracellular accumulation of arsenite by the bacterial cell. Genome sequencing analysis revealed the presence of arsenite resistance as well as other heavy metals/oxyanion resistance genes in the genome of this bacterial strain. Therefore, <i>Halomonas elongata</i> strain SEK2 was identified as an arsenite-resistant halophilic bacterium for the first time that could be used for arsenite bioremediation in saline arsenite-polluted environments.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 6","pages":"1393 - 1409"},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141185845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Threshold and combined effects of heavy metals on the risk of phenotypic age acceleration among U.S. adults","authors":"Yalan Liu,&nbsp;Zhaofeng Jin,&nbsp;Shihao Fu","doi":"10.1007/s10534-024-00609-x","DOIUrl":"10.1007/s10534-024-00609-x","url":null,"abstract":"<div><p>Accumulation of heavy metals in the body has been shown to affect the phenotypic age (PhenoAge). However, the combined and threshold effects of blood heavy metals on the risk of PhenoAge acceleration (PhenoAgeAccel) are not well understood. A cross-sectional study was conducted using blood heavy metal data (<i>N</i> = 7763, age ≥18 years) from the 2015-2018 National Health and Nutrition Examination Survey. PhenoAgeAccel was calculated from actual age and nine biomarkers. Multiple regression equations were used to describe the relationship between heavy metals and PhenoAgeAccel. Least Absolute Shrinkage and Selection Operator (LASSO) regression modeling was used to explore the relationship between the combined effects of heavy metals and PhenoAgeAccel. Threshold effect and multiple regression analyses were performed to explore the linear and nonlinear relationships between heavy metals and PhenoAgeAccel. Threshold effect analysis showed that blood mercury (Hg) concentration was linearly associated with PhenoAgeAccel. In contrast, lead (Pb), cadmium (Cd), manganese (Mn), and combined exposure were nonlinearly associated with PhenoAgeAccel. In addition, the combination of Pb, Cd, Hg, and Mn significantly affected PhenoAgeAccel. The risk of PhenoAgeAccel was increased by 207% (<i>P</i> &lt; 0.0001). Meanwhile, a threshold relationship was found between blood Pb, Cd, Mn, and the occurrence of PhenoAgeAccel. Overall, our results indicate that combined exposure to heavy metals may increase the risk of PhenoAgeAccel. This study underscores the need to reduce heavy metal pollution in the environment and provides a reference threshold for future studies.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1279 - 1288"},"PeriodicalIF":4.1,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141178365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Gentianella acuta (Michx.) Hulten against the arsenic-induced development hindrance of mouse oocytes","authors":"Chunyu Wang,&nbsp;Biao Wang,&nbsp;Ying Wei,&nbsp;Shubin Li,&nbsp;Jingyu Ren,&nbsp;Yanfeng Dai,&nbsp;Gang Liu","doi":"10.1007/s10534-024-00613-1","DOIUrl":"10.1007/s10534-024-00613-1","url":null,"abstract":"<div><p>The current study was designed to investigate the alleviative effect of <i>Gentianella acuta</i> (Michx.) Hulten (<i>G. acuta</i>) against the sodium arsenite (NaAsO₂)-induced development hindrance of mouse oocytes. For this purpose, the in vitro maturation (IVM) of mouse cumulus-oocyte complexes (COCs) was conducted in the presence of NaAsO₂ and <i>G. acuta</i>, followed by the assessments of IVM efficiency including oocyte maturation, spindle organization, chromosome alignment, cytoskeleton assembly, cortical granule (CGs) dynamics, redox regulation, epigenetic modification, DNA damage, and apoptosis. Subsequently, the alleviative effect of <i>G. acuta</i> intervention on the fertilization impairments of NaAsO₂-exposed oocytes was confirmed by the assessment of in vitro fertilization (IVF). The results showed that the <i>G. acuta</i> intervention effectively ameliorated the decreased maturation potentials and fertilization deficiency of NaAsO₂-exposed oocytes but also significantly inhibited the DNA damages, apoptosis, and altered H3K27me3 expression level in the NaAsO₂-exposed oocytes. The effective effects of <i>G. acuta</i> intervention against redox dysregulation including mitochondrial dysfunctions, accumulated reactive oxygen species (ROS) generation, glutathione (GSH) deficiency, and decreased adenosine triphosphate (ATP) further confirmed that the ameliorative effects of <i>G. acuta</i> intervention against the development hindrance of mouse oocytes were positively related to the antioxidant capacity of <i>G. acuta</i>. Evidenced by these abovementioned results, the present study provided fundamental bases for the ameliorative effect of <i>G. acuta</i> intervention against the meiotic defects caused by the NaAsO₂ exposure, benefiting the future application potentials of <i>G. acuta</i> intervention in these nutritional and therapeutic research for attenuating the outcomes of arseniasis.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 6","pages":"1411 - 1430"},"PeriodicalIF":4.1,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, theoretical studies, antibacterial, and antibiofilm activities of novel azo-azomethine chelates against the pathogenic bacterium Proteus mirabilis","authors":"Fatma I. Abouzayed,&nbsp;Rasha Mohammad Fathy,&nbsp;Shimaa G. Hussien,&nbsp;Gharieb S. El-Sayyad,&nbsp;Saeyda A. Abouel-Enein","doi":"10.1007/s10534-024-00608-y","DOIUrl":"10.1007/s10534-024-00608-y","url":null,"abstract":"<div><p>2-((1-(4-((2,4,6-trioxohexahydropyrimidin-5-yl)diazenyl) phenyl) ethylidene) amino) benzoic acid (H₃L), and its V(IV), Co(II), Ni(II), Cu(II), Pd(II) and Ag(I) chelates were synthesized. They were defined using multiple spectral and analytical techniques. With the exception of Ag(I) chelate, all chelates possessed non-electrolytic character. Square pyramidal shape was proposed for V(IV) chelate and Square planar for the other chelates. The analysis of functional group bands of H₃L and its coordination compounds alludes that H₃L chelated as neutral tetradentate via nitrogen atoms of azo and azomethine groups, oxygen atom of carbonyl of barbituric acid and OH of the carboxylic group. TG/DTG predicted the thermal behaviors of all compounds. The antibacterial activity of H₃L and its coordination compounds was conducted against <i>Proteus mirabilis</i> at concentrations of 250, 500, and 1000 µg/mL. Ag(I) at 1000 µg/mL, showed the most inhibiting potency against <i>P. mirabilis</i> and registered zone of inhibition of 28.33 ± 0.84 mm and highest biofilm inhibition of 70.31%. At 50 Gy of gamma irradiation, the reducing effect of Ag(I) chelate was improved. The protein interruption of <i>P. mirabilis</i> was greatly interrupted by increasing the concentration of the chaletes. Also, Ag(I) showed the highest cytotoxicity with IC₅₀ value of 11.5 µg/ mL. The novelty of this study is the synthesis of a new azo-Schiff base and this is almost the first publication of the effect of azo-Schiff ligands against that bacterial strain <i>P. mirabilis</i>.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"37 5","pages":"1255 - 1278"},"PeriodicalIF":4.1,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}