the Indian Journal of Pharmacy最新文献

{"title":"Comments on the efficacy of paracetamol in the management of hemodynamically significant patent ductus arteriosus in preterm newborns.","authors":"Bhagyashree Sharma, Saniya Shah, Suresh Dange, Bhagyashree Dange","doi":"10.4103/ijp.ijp_716_24","DOIUrl":"10.4103/ijp.ijp_716_24","url":null,"abstract":"","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"446"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-derived stem cells exosomal circHIPK3 protects ovarian function by regulating MAPK signaling.","authors":"Wei Zhao, Da Erhan, Shujun Liu, Liyan Zhang, Caizhu Hai, Yanan Zhang, Haiyan Li, Hongwu Wang, Caisheng Wang","doi":"10.4103/ijp.ijp_499_24","DOIUrl":"10.4103/ijp.ijp_499_24","url":null,"abstract":"<p><strong>Background: </strong>Exosomes derived from adipose-derived stem cells (ADSCs) have garnered significant attention for their therapeutic potential in various diseases. These vesicles are capable of transporting bioactive molecules such as noncoding RNAs and proteins. Among these noncoding RNAs, circular RNAs (circRNAs) are characterized as end-to-end circular structures, which are notably enriched within exosomes.</p><p><strong>Objective: </strong>This study aims to investigate the impact of the circHIPK3 delivered via ADSC-derived exosomes on ovarian aging.</p><p><strong>Materials and methods: </strong>ADSCs were isolated, and exosomes were obtained from a cell culture medium. The exosomes were labeled with PKH26, and uptake by primary granulosa cells (pGCs) was detected. ADSCs were transfected with circHIPK3 siRNAs, and the exosomes were isolated for the treatment of aging female mice. Ovary weight was recorded, and HE staining, Masson's trichrome, and TUNEL staining were performed to detect tissue morphology and apoptosis in ovary tissues. In addition, the senescence and apoptosis of pGCs were evaluated using the S-β-gal staining kit and Annexin V/PI detection kit. Further experiments included immunoprecipitation and RNA pulldown, determined the ubiquitination of p38 protein under circHIPK3 alteration.</p><p><strong>Results: </strong>Results showed that ADSC-derived exosomes effectively delivered circHIPK3 to pGCs. Treatment with these exosomes significantly increased ovary weight and enhanced follicular development in aged mice. Conversely, the depletion of circHIPK3 reversed these effects, promoting cell apoptosis. ADSC-derived exosomes also mitigated senescence and apoptosis in pGCs, while circHIPK3 depletion hindered these benefits.</p><p><strong>Conclusion: </strong>Exosomal circHIPK3 modulated the ubiquitination of p38 in pGCs to improve ovarian function in aging mice and to promote pGC cell viability.</p>","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"411-419"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the anti-bacterial activities of potentized medicines on the clinical isolates from the urine samples.","authors":"Normi Gajjar, Gitanjali Talele, Gaurang B Shah, Rajesh Shah","doi":"10.4103/ijp.ijp_517_24","DOIUrl":"10.4103/ijp.ijp_517_24","url":null,"abstract":"<p><strong>Background: </strong>Our study explores nosodes (potentized medicines derived from microbes), as alternative antimicrobial measures against infections. Different nosodes were tested for efficacy against pathogens in clinical urine samples, comparing them with Gentamicin.</p><p><strong>Objective: </strong>Evaluating the efficacy of potentized medicines against microbes found in urine samples.</p><p><strong>Materials and methods: </strong>The test samples were added to the sterile 96 well plates with 150 μL of sterile media followed by bacteria-positive clinical urine samples (10 μL), and the plate was incubated at 37°C. After 72 h, optical density was measured. Reduction in bacterial growth was calculated as the antibacterial potential of the drugs.</p><p><strong>Results: </strong>Escherichia coli poly-nosode 100C exhibited the highest efficacy at 93.79%, followed by Neisseria gonorrhoeae 100C (89.27%), E. coli 200C (79.61%), and Syphilinum 30C (78.76%) against Klebsiella pneumoniae. The average efficacy against Pseudomonas aeruginosa for the 27 test samples studied was 72.32%, (verses Gentamicin 74.25%). The highest efficacy at 92.79% was observed with E. coli poly-nosode followed by methicillin-resistant Staphyloccus aureus (MRSA) 30C (92.29%), N. gonorrhoeae 50C (92.29%), Cantharis 30C (90.95%), and Salmonella typhi 30C poly-nosode (81.35%). Cantharis 30C at 86.53% and E. coli 200C at 78.02% were effective against the mixed infection of E. coli + Candida albicans. The average efficacy of nosodes against this mixed infection was 63.53%, while Gentamicin demonstrated 61.97% efficacy. MRSA 100C showed the highest efficacy at 52.37% against E. coli, and an average efficacy of 44.5% was noted with test samples.</p><p><strong>Conclusions: </strong>Our study unveils the therapeutic potential of homeopathic medicines against antibiotic-resistant bacterial strains.</p>","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"405-410"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mirabegron-induced acute urticaria: An uncommon cutaneous adverse drug reaction.","authors":"Sambit Chatterjee, Anupam Das","doi":"10.4103/ijp.ijp_393_24","DOIUrl":"10.4103/ijp.ijp_393_24","url":null,"abstract":"","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"444-445"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulse methylprednisolone-induced sinus bradycardia: A rare but must know association.","authors":"Ashna Kumar, Pradeep Kumar Gunasekaran, Sarbesh Tiwari, Daisy Khera, Lokesh Saini","doi":"10.4103/ijp.ijp_261_24","DOIUrl":"10.4103/ijp.ijp_261_24","url":null,"abstract":"","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"440-441"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ensuring medication and patient safety for better quality healthcare.","authors":"Lalit Sharma, Ajay Prakash, Bikash Medhi","doi":"10.4103/ijp.ijp_109_25","DOIUrl":"10.4103/ijp.ijp_109_25","url":null,"abstract":"","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"375-378"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimethyl fumarate improves nuclear factor erythroid-related factor 2-mediated antioxidant response to ameliorate functional and molecular deficits in experimental diabetic neuropathy.","authors":"Amrutha Jindam, Anil Kumar Kalvala, Vijay Kumar Arruri, Sourav Das, Ashutosh Kumar","doi":"10.4103/ijp.ijp_540_24","DOIUrl":"10.4103/ijp.ijp_540_24","url":null,"abstract":"<p><strong>Objectives: </strong>In the present study, we tested the functional/pharmacological significance of dimethyl fumarate (DMF) in streptozotocin-induced diabetic neuropathy (DN) in rats and high glucose-exposed Neuro2a (N2a) cells.</p><p><strong>Materials and methods: </strong>To evaluate the pharmacological effects of DMF on diabetic neuropathy, we assessed behavioral and functional parameters of peripheral neuropathy, oxidative stress markers, and target protein expression using immunohistochemistry/immunocytochemistry, and Western blotting in diabetic rats and hyperglycemic N2a cells.</p><p><strong>Results: </strong>Diabetic rats exhibited hyperalgesia, allodynia, and compromised sensory and motor nerve conduction velocities in comparison to normal rats. Dorsal root ganglias of diabetic rats showed decreased antioxidant levels and increased pro-inflammatory transcription factors such as nuclear factor erythroid-related factor 2 and nuclear factor-kappa B, alongside reduced expression of the heat shock protein (HSP) 90. Administering DMF to diabetic rats for 2 weeks reversed these effects in a dose-dependent manner. We observed significant compromise in mitochondrial function, indicated by reduced mitochondrial membrane potential, increased free radical levels, and compromised mitochondrial complex activities in N2a cells exposed to elevated glucose levels. Conversely, DMF treatment restored mitochondrial function and augmented mitochondrial biogenesis through the upregulation of PGC-1α and improved chaperone activity by increasing the expression of HSP 60 and HSP 70.</p><p><strong>Conclusions: </strong>Overall, DMF alleviated neurobehavioral deficits in DN rats and enhanced mitochondrial function and chaperone activity under hyperglycemic conditions in both diabetic rats and N2a cells.</p>","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"386-395"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of sunflower seed oil against Alzheimer's disease through experimental and network pharmacology studies.","authors":"Yashodhan Desai, Deepak Karunakaran, Juhi Singh, Alric Raul Noronha, Gagan Poojary, Bijaya Chettri, Rekha Shenoy, Madhavan Nampoothiri, Sree Lalitha Bojja","doi":"10.4103/ijp.ijp_761_23","DOIUrl":"10.4103/ijp.ijp_761_23","url":null,"abstract":"<p><strong>Objectives: </strong>With the prevalence of Alzheimer's disease (AD) increasing exponentially, there has been a shift in the focus of drug discovery for AD from treating the symptoms to preventing the development of the disease. Several natural compounds are extensively studied as neuroprotectives in preventing disease progression. Helianthus annuus seed oil (HA) is widely used as cooking oil and is abundant in antioxidant activity. Therefore, we evaluated the effect of HA in mice model of scopolamine-induced amnesia and explored the potential underlying mechanisms.</p><p><strong>Methods: </strong>Twenty-four male mice were administered orally with either distilled water (control and scopolamine groups) or treatment groups (HA 100 and HA 200 mg/kg) for 8 consecutive days. All groups, except the control group, received an intraperitoneal injection of scopolamine at a dose of 1 mg/kg. Subsequently, novel object recognition task for cognition assessment and open field tests for locomotory activity were performed. In addition, network analysis was performed to identify the key bioactives and targets of HA against AD. Further, the binding affinity of HA bioactives to the key targets was verified by molecular docking analysis.</p><p><strong>Results: </strong>HA (100 mg/kg and 200 mg/kg) significantly ameliorated recognition memory compared to the scopolamine group, suggesting the protective effect of HA against cognitive impairment. Network analysis indicated that the key bioactives of HA, chlorogenic acid, and oleic acid act through multiple targets and pathways, particularly the mitogen-activated protein kinase (MAPK) pathway, to ameliorate AD symptoms. Importantly, chlorogenic acid showed good binding affinity with MAPKs, TP53, and EP300.</p><p><strong>Conclusion: </strong>HA has therapeutic benefits in AD acting through the MAPK pathway. However, further studies need to be done to confirm the results derived and translate the potential use of HA as a dietary supplement for preventing AD.</p>","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"396-404"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using the Physitrack telehealth platform for enhancing pharmacology education in pain management.","authors":"Ankit K Badge, Rashi Lahoti, Nandkishor J Bankar, Gulshan Bandre","doi":"10.4103/ijp.ijp_717_24","DOIUrl":"10.4103/ijp.ijp_717_24","url":null,"abstract":"","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"442-443"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putrescine can inhibit germinal center B cell differentiation by inducing reactive oxygen species generation.","authors":"Yuqiang Zhang, Peijia Cong, Bin Wang, Haifeng Lian, Yuming Zhou","doi":"10.4103/ijp.ijp_531_24","DOIUrl":"10.4103/ijp.ijp_531_24","url":null,"abstract":"<p><strong>Abstract: </strong>Polyamine synthesis and abnormal regulation of B cell differentiation occur concurrently in various diseases. We investigated whether putrescine could suppress germinal center B cell (GCB) differentiation by inducing reactive oxygen species (ROS) generation. The results of flow cytometry analysis revealed that putrescine did not affect B cell apoptosis and cell cycle. The results of RT-qPCR and western blotting revealed that putrescine could inhibit CD79a phosphorylation rather than total expression. Using an O2K high-resolution respirometer, we illustrated that putrescine increased the oxygen consumption rate in the basal mitochondrial respiration stage, ATP-coupled respiration stage, and maximum respiration stage. Similarly, it also elevated ROS generation across stages in B cells and reduced the proportion of GCB cells. Meanwhile, ROS scavenging by SOD could reverse such inhibitory effects on GCB cells. We concluded that putrescine could inhibit the differentiation of GCB cells by reducing CD79a phosphorylation and increasing ROS levels in GCB cells.</p>","PeriodicalId":49189,"journal":{"name":"the Indian Journal of Pharmacy","volume":"56 6","pages":"430-436"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}