Science in China. Series C, Life Sciences / Chinese Academy of Sciences最新文献_第6页

Efficient expression of codon-adapted human acetaldehyde dehydrogenase 2 cDNA with 6xHis tag in Pichia pastoris. 带6xHis标签的密码子适应性人乙醛脱氢酶2 cDNA在毕赤酵母中的高效表达

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0134-0

YuFeng Zhao, MingKe Lei, YuanXin Wu, ZiSheng Zhang, CunWen Wang

{"title":"Efficient expression of codon-adapted human acetaldehyde dehydrogenase 2 cDNA with 6xHis tag in Pichia pastoris.","authors":"YuFeng Zhao, MingKe Lei, YuanXin Wu, ZiSheng Zhang, CunWen Wang","doi":"10.1007/s11427-009-0134-0","DOIUrl":"https://doi.org/10.1007/s11427-009-0134-0","url":null,"abstract":"Human mitochondrial acetaldehyde dehydrogenase 2 (ALDH2) catalyzes the oxidation of acetaldehyde to acetic acid. Therefore, ALDH2 has therapeutic potential in detoxification of acetaldehyde. Furthermore, ALDH2 catalyzes nitroglycerin to nitrate and 1, 2-glyceryldinitrate during therapy for angina pectoris, myocardial infarction, and heart failure. Large quantities of ALDH2 will be needed for potential clinical practice. In this study, Pichia pastoris was used as a platform for expression of human ALDH2. Based on the ALDH2*1 cDNA sequence, we designed ALDH2 cDNA by choosing the P. pastoris preferred codons and by decreasing the G + C content level. The sequence was synthesized using the overlap extension PCR method. The cDNA and 6xHis tags were subcloned into the plasmid pPIC9K. The recombinant protein was expressed in P. pastoris GS115 and purified using Ni(2+)-Sepharose affinity chromatography. The amount of secreted protein in the culture was 80 mg/L in shake-flask cultivation and 260 mg/L in high-density bioreactor fermentation. Secreted ALDH2 was easily purified from the culture supernatant by using Ni(2+)-Sepharose affinity chromatography. After purification of the fermentation supernatant, the enzyme had a specific activity of 1.2 U/mg protein. The yield was about 16 mg/L in a shake flask culture of P. pastoris GS115 which contained the original human ALDH2*1 cDNA.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"935-41"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0134-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Threat and management strategies of potentially invasive insects in China. 中国潜在入侵昆虫的威胁与管理策略。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0126-0

RunZhi Zhang, YaPing Zhang, YouXu Jiang

{"title":"Threat and management strategies of potentially invasive insects in China.","authors":"RunZhi Zhang, YaPing Zhang, YouXu Jiang","doi":"10.1007/s11427-009-0126-0","DOIUrl":"https://doi.org/10.1007/s11427-009-0126-0","url":null,"abstract":"The Global Invasive Species Database, GISD, comprises 27 species of the most significant invasive alien insects in the world (through November, 2005), 6 of which are originally native to China, 11 are established in China, and 10 have a potential invasion threat to China. This paper discusses these species in terms of distribution, harmfulness and dispersal ways, and finds that: (i) Information regarding invasive insects in the GISD remains inadequate. Such harmful invasive species as Opogona sacchari (Bojer), Oracella acuta (Lobdell), and Dendroctonus valens LeConte are not included. (ii) Ten species of invasive insects, particularly Lasius neglectus Van Loon and Linepithema humile (Mayr) which become established in areas near China, have the potential to become established in China. (iii) Special attention should be paid to species from Asia and the Americas because of their greater likelihood of becoming established in China. Finally, some management strategies including legislation, quarantine, early warning, prevention and control are suggested.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"903-10"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0126-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

豌豆质膜内源CDPK对植物转脂蛋白CaMBP-10的磷酸化及CaMBP-10对激酶自磷酸化的影响豌豆质膜内源CDPK对植物转脂蛋白CaMBP-10的磷酸化及CaMBP-10对激酶自磷酸化的影响

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-20 DOI: 10.1360/ZC2009-39-9-862

赵玉龙, 谢万钦, 胡文全, 张映熠, 张蕊, 李翠凤

{"title":"豌豆质膜内源CDPK对植物转脂蛋白CaMBP-10的磷酸化及CaMBP-10对激酶自磷酸化的影响","authors":"赵玉龙, 谢万钦, 胡文全, 张映熠, 张蕊, 李翠凤","doi":"10.1360/ZC2009-39-9-862","DOIUrl":"https://doi.org/10.1360/ZC2009-39-9-862","url":null,"abstract":"植物转脂蛋白(LTPs)是多基因编码的蛋白家族, 广泛分布于高等植物. 虽然LTPs的确切功能至今仍不完全清楚, 但它参与植物生物、非生物胁迫反应以及它的抗性功能已成为近年来的研究热点. 关于LTPs功能的调节机制目前几乎一无所知. 最近, 从白菜中分离的钙调素结合蛋白-10(CaMBP-10)被鉴定为植物转脂蛋白家族成员, 并且, 体外实验证明钙调素(CaM)调节其脂质结合活性. 为了深入了解转脂蛋白功能的调节机制, 本文研究了CaMBP-10的磷酸化作用, 发现 CaMBP-10 可被豌豆质膜内源性蛋白激酶磷酸化, 钙离子(Ca2+)能刺激磷酸化, 钙螯合剂EGTA以及CaM拮抗剂W-7和TFP 均能显著抑制磷酸化. 免疫印迹分析最终确定该激酶为CDPK家族成员. 构建突变体进一步研究了CaMBP-10的磷酸化位点, 发现其位于蛋白的C-末端区域, 并与已确定的CaM结合位点重合. 同时, 分析结果表明CaM能抑制CaMBP-10的磷酸化. 反之, CaMBP-10的磷酸化又能阻断其与CaM的结合, 显示出两种调节方式相互竞争的特点. 为深入研究磷酸化作用对CaMBP-10脂质结合活性的影响, 构建突变体(Ser83Asp, Ser85Asp)以模拟磷酸化状态. 实验结果显示, 磷酸化作用能显著增强CaMBP-10的脂质结合活性, 而且突变体的脂质结合活性不受CaM的影响. 采用胶内磷酸化测定法(in-gel kinase assay)研究了激酶的自磷酸化特点以及CaMBP-10对激酶自磷酸化的影响, 发现CaMBP-10能激活激酶的自磷酸化, 激酶的自磷酸化又能促进其对底物的磷酸化作用. 这样, 激酶的自磷酸化与底物的磷酸化形成一种“正反馈环”的调节模式. 综合研究结果, 本文首次证明了LTP受CaM结合和CDPK磷酸化的双重调节. 而且, CaM结合位点与磷酸化位点的重合预示可能存在特殊的调节机制, 以协同应答胞内的Ca2+信号.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"6 1","pages":"862-872"},"PeriodicalIF":0.0,"publicationDate":"2009-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89519104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caveolin-1 基因沉默促进乳腺上皮细胞ERα36 介导的PI3K/AKT信号通路的激活 Caveolin-1 基因沉默促进乳腺上皮细胞ERα36 介导的PI3K/AKT信号通路的激活

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-20 DOI: 10.1360/ZC2009-39-9-830

封爽, 王洋, 王曦, 王兆一, 崔玉影, 刘晶, 赵春晖, 金梅, 邹伟

引用次数: 0

Characterization of SoxB2 and SoxC genes in amphioxus (Branchiostoma belcheri): implications for their evolutionary conservation. 文昌鱼SoxB2和SoxC基因的特征及其进化保护意义

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0111-7

YuShuang Lin, DongYan Chen, QiuSheng Fan, HongWei Zhang

{"title":"Characterization of SoxB2 and SoxC genes in amphioxus (Branchiostoma belcheri): implications for their evolutionary conservation.","authors":"YuShuang Lin, DongYan Chen, QiuSheng Fan, HongWei Zhang","doi":"10.1007/s11427-009-0111-7","DOIUrl":"https://doi.org/10.1007/s11427-009-0111-7","url":null,"abstract":"Most Sox genes directly affect cell fate determination and differentiation. In this study, we isolated two Sox genes: SoxB2 and SoxC from amphioxus (Branchiostoma belcheri), the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches, suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation, they are expressed in the neural ectoderm and archenterons. In adults, they are expressed not only in the nerve cord, but also in the gut, midgut diverticulum, gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 9","pages":"813-22"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0111-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40054515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter. 高效的LEC2激活OLEOSIN表达需要两个相邻的RY元件在其启动子上。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0119-z

NanYing Che, Yang Yang, YanDong Li, LiLi Wang, Ping Huang, Yin Gao, ChengCai An

{"title":"Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter.","authors":"NanYing Che, Yang Yang, YanDong Li, LiLi Wang, Ping Huang, Yin Gao, ChengCai An","doi":"10.1007/s11427-009-0119-z","DOIUrl":"https://doi.org/10.1007/s11427-009-0119-z","url":null,"abstract":"As the main structural protein of oil body, OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here, we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN, responsible for seed specificity and LEC2 activation, was determined by 5'-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays, mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity, while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore, we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 9","pages":"854-63"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0119-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40054521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

HIV-specific CD8+ T cell responses to HXB2 Gag and Nef peptide pools in Chinese HIV/AIDS patients. HIV特异性CD8+ T细胞对中国HIV/AIDS患者HXB2 Gag和Nef肽库的应答

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0117-1

HongWei Zhang, ZhiFeng Qiu, Yang Jiao, AiXia Wang, TaiSheng Li

{"title":"HIV-specific CD8+ T cell responses to HXB2 Gag and Nef peptide pools in Chinese HIV/AIDS patients.","authors":"HongWei Zhang, ZhiFeng Qiu, Yang Jiao, AiXia Wang, TaiSheng Li","doi":"10.1007/s11427-009-0117-1","DOIUrl":"https://doi.org/10.1007/s11427-009-0117-1","url":null,"abstract":"HXB2 is primarily used as a template strain in developing HIV vaccines in Europe and the US. However, it is not yet known whether the strain can induce strong HIV-specific CD8+ T cell responses in Chinese HIV/AIDS patients. In the present study, two groups of subjects were investigated: 9 AIDS patients and 7 long-term nonprogressors (LTNPs). HIV-specific CD8+ T cell responses were examined in all patients through the ELISPOT assay. CD4+ T cell counts, CD8+ T cell counts, viral load and HIV subtype of each patient were also measured. Thailand B virus strain was identified among all the patients. The breadth and magnitude of HIV-specific CD8+ T cell responses in the LTNPs group are greater than those in the AIDS group (P<0.01). There is a positive correlation between magnitude of HIV-specific CD8+ T cell responses and CD4+ T cells, and a negative correlation between HIV-specific CD8+ T cell responses and mean viral load. In summary, the HIV-specific CD8+ T cell responses to the HXB2 Gag and Nef peptide pools are considerable in Chinese HIV/AIDS patients infected with Thailand B virus strain. HIV-1 vaccines based on HXB2 strain that can induce extensive immunity may be helpful for Chinese.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 9","pages":"841-6"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0117-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40054519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Expression pattern of antibacterial genes in the Musca domestica. 家蝇抗菌基因的表达模式。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0121-5

Yan Wang, XiaoBao Jin, JiaYong Zhu, AiHua Zeng, FuJiang Chu, XiaoRong Yang, Yan Ma

引用次数: 8

Protective effect of liver ischemic preconditioning on rat hepatocytes. 肝缺血预处理对大鼠肝细胞的保护作用。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0113-5

JianZhu Fu, Yu Wang, LiJun Zhang, ZeLi Yu

引用次数: 1

Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.). 一个水稻卷叶突变体的遗传分析与基因定位。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-09-01 Epub Date: 2009-10-06 DOI: 10.1007/s11427-009-0109-1

YongFeng Shi, Jie Chen, WenQiang Liu, QiNa Huang, Bo Shen, Hei Leung, JianLi Wu

{"title":"Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.).","authors":"YongFeng Shi, Jie Chen, WenQiang Liu, QiNa Huang, Bo Shen, Hei Leung, JianLi Wu","doi":"10.1007/s11427-009-0109-1","DOIUrl":"https://doi.org/10.1007/s11427-009-0109-1","url":null,"abstract":"To understand the development of rice leaf blades, we identified a new rolled-leaf mutant, w32, from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene, tentatively named rl11(t), is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers, the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 9","pages":"885-90"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0109-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40054466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25