Science in China. Series C, Life Sciences / Chinese Academy of Sciences最新文献_第5页

Expression and subcellular localization of mechano-growth factor in osteoblasts under mechanical stretch. 机械拉伸作用下成骨细胞机械生长因子的表达及亚细胞定位。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0122-4

BingBing Zhang, ChengYu Xian, YanFeng Luo, YuanLiang Wang

引用次数: 7

The conflict adaptation is affected by attentional strategies: evidence from the arrow flanker task. 注意策略对冲突适应的影响:来自箭侧任务的证据。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0123-3

Jia Chen, Chang Li, ChunHong He, AnTao Chen

引用次数: 6

Identification of in vivo induced protein antigens of Salmonella enterica serovar Typhi during human infection. 人感染伤寒沙门氏菌血清型体内诱导蛋白抗原的鉴定。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0127-z

Yong Hu, YanGuang Cong, Shu Li, XianCai Rao, Gang Wang, FuQuan Hu

{"title":"Identification of in vivo induced protein antigens of Salmonella enterica serovar Typhi during human infection.","authors":"Yong Hu, YanGuang Cong, Shu Li, XianCai Rao, Gang Wang, FuQuan Hu","doi":"10.1007/s11427-009-0127-z","DOIUrl":"https://doi.org/10.1007/s11427-009-0127-z","url":null,"abstract":"During infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. Hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. We used in vivo induced antigen technology (IVIAT) to identify antigens which are expressed during infection with Salmonella enterica serovar Typhi. We identified 7 in vivo induced (IVI) antigens, which included BcfD (a fimbrial structural subunit), GrxC (a glutaredoxin 3), SapB (an ABC-type transport system), T3663 (an ABC-type uncharacterized transport system), T3816 (a putative rhodanese-related sulfurtransferase), T1497 (a probable TonB-dependent receptor) and T3689 (unknown function). Of the 7 identified antigens, 5 antigens had no cross-immunoreactivity in adsorbed control sera from healthy subjects. These 5 included BcfD, GrxC, SapB, T3663 and T3689. Antigens identified in this study are potential targets for drug and vaccine development and may be utilized as diagnostic agents.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"942-8"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0127-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Expression of rice dwarf phytoreovirus Pns6 and the specificity analysis of its monoclonal antibodies. 水稻矮化植物呼肠孤病毒Pns6的表达及其单克隆抗体的特异性分析。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0129-x

Xu Ji, ChunHong Wei, Yi Li

{"title":"Expression of rice dwarf phytoreovirus Pns6 and the specificity analysis of its monoclonal antibodies.","authors":"Xu Ji, ChunHong Wei, Yi Li","doi":"10.1007/s11427-009-0129-x","DOIUrl":"https://doi.org/10.1007/s11427-009-0129-x","url":null,"abstract":"The genome of rice dwarf phytoreovirus (RDV) is composed of 12 double-stranded RNA segments, of which segment S6 encodes a non-structural protein Pns6 identified as the movement protein. In this report, Pns6 with a 6-histidine tag at the N-terminal was expressed in E. coli after induction under low temperature (18 degrees C) and low concentration (0.4 mmol/L and 0.2 mmol/L) of IPTG, and then purified by Ni-chelated affinity chromatography. Stability analysis indicated that the expressed HisPns6 protein was stable at 37 degrees C after 24 h treatment. This recombinant protein was then used to make monoclonal antibody. Total 18 hybridoma clones were obtained. The specificity of antibodies was tested by Western blot using native Pns6 extracted from RDV-infected rice leaves, and 15 positive clones were confirmed. Mapping of the antigenic sites of Pns6 using antibodies showed that the most sensitive antigen determinant is located in the C-terminal region (the 296th-509th amino acids) of Pns6, which is confirms bioinformatics analysis.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"958-64"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0129-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization and function analysis of a Halo-alkaline-adaptable Trk K+ uptake system in Alkalimonas amylolytica strain N10. 溶淀粉碱单胞菌N10耐碱性Trk - K+吸收系统的特性及功能分析

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0132-2

YongHao Guo, YanFen Xue, Jun Liu, QuanHui Wang, YanHe Ma

{"title":"Characterization and function analysis of a Halo-alkaline-adaptable Trk K+ uptake system in Alkalimonas amylolytica strain N10.","authors":"YongHao Guo, YanFen Xue, Jun Liu, QuanHui Wang, YanHe Ma","doi":"10.1007/s11427-009-0132-2","DOIUrl":"https://doi.org/10.1007/s11427-009-0132-2","url":null,"abstract":"By functional complementation of Escherichia coli mutants defective in potassium (K(+)) uptake, two genes that are required for K(+) uptake in halo-alkaliphilic Alkalimonas amylolytica strain N10 were cloned. These two genes, Aa-trkA (1337 bp) and Aa-trkH (1452 bp), were adjacent on the A. amylolytica N10 chromosome and transcribed in opposite directions. Complementation experiments revealed that Aa-TrkA and Aa-TrkH from A. amylolytica strain N10 restored the ability to grow at low K(+) concentration in E. coli DeltatrkA and DeltatrkG DeltatrkH strains, respectively. In addition, Aa-TrkAH supported the growth of an E. coli DeltasapD strain, indicating that the ATP-binding protein TrkE was dispensable for the Trk system of A. amylolytica strain N10. The net K(+) uptake was detected at different pH levels and the critical NaCl concentration indicated that Aa-TrkAH is an alkaline-adaptable and partially halo-adaptable K(+) transporter. Kinetics determined by heterogeneous K(+) transport experiments with an E. coli DeltatrkA strain revealed that Aa-TrkAH has an alkaline pH optimum close to 8.5 or higher. Site-directed mutagenesis of Aa-TrkH showed that Phe103 and Ser229 play certain key roles in K(+) selection and transportation. The molecular chaperones groES-groEL and tig promoted Aa-TrkH and Aa-TrkA overexpression in vitro.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"949-57"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0132-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.). 太空飞行对甘草（Glycyrrhiza uralensis Fisch.）DNA 变异和次生代谢物的影响。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0120-6

WenYuan Gao, KeFeng Li, Shuo Yan, XiuMei Gao, LiMin Hu

{"title":"Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.).","authors":"WenYuan Gao, KeFeng Li, Shuo Yan, XiuMei Gao, LiMin Hu","doi":"10.1007/s11427-009-0120-6","DOIUrl":"10.1007/s11427-009-0120-6","url":null,"abstract":"Licorice (Glycyrrhiza uralensis Fisch.) seeds were flown on a recoverable satellite for 18 days(the average radiation dose in the flight recovery module was 0.102 mGy/d, the distance from flight apogee to earth was 350 km, gravity 10(-6)). After returning to earth, the seeds were germinated and grown to maturity. The parallel ground-based seeds were also planted under the same conditions. The leaves of licorice were used for inter-simple sequence repeat (ISSR) analysis and the two main secondary metabolites in one-year-old roots were analyzed by high performance liquid chromatography (HPLC). Among 22 random primers used in this experiment, 6 primers generated different DNA band types. Analysis of HPLC showed that the content of glycyrrhizic acid (GA) and liquiritin (LQ) in the roots from seeds flown in space was respectively 2.19, 1.18 times higher than that of the control group. The results demonstrated that the extraterrestrial environment induced mutagenic effects on licorice and affected its secondary metabolites. These changes indicated that extraterrestrial orbit is possible means of breeding of licorice so as to preserve this endangered medicinal plant.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"977-81"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PCA-based method for ancestral informative markers selection in structured populations. 基于pca的结构化群体祖先信息标记选择方法。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0128-y

Feng Zhang, Lei Zhang, Hong-Wen Deng

{"title":"A PCA-based method for ancestral informative markers selection in structured populations.","authors":"Feng Zhang, Lei Zhang, Hong-Wen Deng","doi":"10.1007/s11427-009-0128-y","DOIUrl":"https://doi.org/10.1007/s11427-009-0128-y","url":null,"abstract":"Identification of population structure can help trace population histories and identify disease genes. Structured association (SA) is a commonly used approach for population structure identification and association mapping. A major issue with SA is that its performance greatly depends on the informativeness and the numbers of ancestral informative markers (AIMs). Present major AIM selection methods mostly require prior individual ancestry information, which is usually not available or uncertain in practice. To address this potential weakness, we herein develop a novel approach for AIM selection based on principle component analysis (PCA), which does not require prior ancestry information of study subjects. Our simulation and real genetic data analysis results suggest that, with equivalent AIMs, PCA-based selected AIMs can significantly increase the accuracy of inferred individual ancestries compared with traditionally randomly selected AIMs. Our method can easily be applied to whole genome data to select a set of highly informative AIMs in population structure, which can then be used to identify potential population structure and correct possible statistical biases caused by population stratification.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"972-6"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0128-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Leptospirosis distribution related to freshwater habitats in the Vojvodina region (Republic of Serbia). 与伏伊伏丁那地区(塞尔维亚共和国)淡水生境有关的钩端螺旋体病分布。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0124-2

Zorica Svircev, Slobodan B Marković, Jovan Vukadinov, Sandra Stefan-Mikić, Maja Ruzić, Radoslava Doder, Milotka Fabri, Grozdana Canak, Vesna Turkulov, Dejan B Stojanović, Mirko Draganić

{"title":"Leptospirosis distribution related to freshwater habitats in the Vojvodina region (Republic of Serbia).","authors":"Zorica Svircev, Slobodan B Marković, Jovan Vukadinov, Sandra Stefan-Mikić, Maja Ruzić, Radoslava Doder, Milotka Fabri, Grozdana Canak, Vesna Turkulov, Dejan B Stojanović, Mirko Draganić","doi":"10.1007/s11427-009-0124-2","DOIUrl":"https://doi.org/10.1007/s11427-009-0124-2","url":null,"abstract":"The retrospective study (2002-2007) for human leptospirosis in Vojvodina was undertaken in order to describe the distribution of the disease in relation with some environmental factors. Regarding the presented results, the major detected number of leptospirosis cases concurs with stagnant waters, wetlands, fish pond areas and protected regions, which comprised the basis for mapping of the region in three risk zones: very high risk (incidence rate higher than 5.0), high risk (2.5-5.0) and medium risk of leptospirosis infection (1.0-2.5). During the investigated period, 97 cases were registered with an average of 13.85 cases per year: 2002, 32 cases; 2003, 7; 2004, 22; 2005, 16; 2006, 4 and 2007, 16. Out of these 97 cases only 5 were women. Serovars from 11 presumptive serogroups caused infection, with a predominance of Icterohaemorrhagiae and Bratislava, accounting for 72.72% of cases together. Icterohaemorrhagiae was the commonest infecting serogroup mostly connected with fish ponds. Case fatality ratio was 9.4%.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 10","pages":"965-71"},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0124-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28506560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

A quantification model for apoptosis in mouse embryos in the early stage of fetation. 胚胎早期小鼠胚胎细胞凋亡定量模型的建立。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0130-4

PengFei Wang, JianHua Fu, WanYun Ma, DieYan Chen, DanYu Lü, WenJia Bai

引用次数: 1

APOBEC deaminases-mutases with defensive roles for immunity. 具有免疫防御作用的 APOBEC 脱氨酶--突变体。

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-10-01 Epub Date: 2009-11-13 DOI: 10.1007/s11427-009-0133-1

Courtney Prochnow, Ronda Bransteitter, XiaoJiang S Chen

引用次数: 0