机械拉伸作用下成骨细胞机械生长因子的表达及亚细胞定位。

BingBing Zhang, ChengYu Xian, YanFeng Luo, YuanLiang Wang
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引用次数: 7

摘要

机械生长因子(Mechano-growth factor, MGF)是肌细胞中的一种拉伸敏感因子,其它机械细胞在机械刺激下也可能产生。本研究检测了MGF在拉伸成骨细胞中mRNA和蛋白的表达。定量分析表明,循环拉伸刺激使MGF mRNA和蛋白表达从低基础水平快速急剧升高;mRNA和蛋白水平分别在6和12 h达到峰值,为基础水平的5和5.2倍,24 h恢复正常。免疫荧光分析显示MGF蛋白的亚细胞分布局限于细胞核。我们的结论是,循环拉伸刺激可以以脉冲方式诱导成骨细胞中MGF的表达;MGF的核分布提示MGF可能作为自分泌生长因子在机械细胞中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression and subcellular localization of mechano-growth factor in osteoblasts under mechanical stretch.

Mechano-growth factor (MGF) is a stretch sensitive factor in myocytes, and it might also be produced by other mechanocytes under mechanical stimulation. In this study, both the mRNA and protein expression of MGF were detected in stretched osteoblasts. Quantitative analysis showed that a cyclic stretching stimulation caused a quick and sharp increase of MGF mRNA and protein expression from a low basal level under no stretch; the mRNA and protein levels respectively peaked in 6 and 12 h to 5 and 5.2 fold over the basal level and returned to normal by 24 h. The subcellular distribution of MGF protein was revealed by immunofluorescence analysis to be restricted to the nucleus. We concluded that cyclic stretching stimulation could induce MGF expression in osteoblasts in a pulsing fashion; and the nuclear distribution of MGF suggested that MGF might act in mechanocytes as an autocrine growth factor.

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