Archaea-An International Microbiological Journal最新文献

{"title":"Thermophilic Solid-State Anaerobic Digestion of Corn Straw, Cattle Manure, and Vegetable Waste: Effect of Temperature, Total Solid Content, and C/N Ratio.","authors":"Lianghu Su, Xu Sun, Chenwei Liu, Rongting Ji, Guangyin Zhen, Mei Chen, Longjiang Zhang","doi":"10.1155/2020/8841490","DOIUrl":"10.1155/2020/8841490","url":null,"abstract":"<p><p>Thermophilic solid-state anaerobic digestion (SS-AD) of agricultural wastes, i.e., corn straw, cattle manure, and vegetable waste, was carried out in this study. The effects of temperature (40-60°C), initial solid content (ISC, 17.5-32.5%), and C/N ratio (15-32 : 1) on biogas production were evaluated using a Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). The results showed that optimization of process parameters is important to promote the SS-AD performance. All the factors, including interactive terms (except the ISC), were significant in the quadratic model for biogas production with SS-AD. Among the three operation parameters, the C/N ratio had the largest effect on biogas production, followed by temperature, and a maximum biogas yield of 241.4 mL gVS^-1 could be achieved at 47.3°C, ISC = 24.81%, and C/N = 22.35. After 20 d of SS-AD, the microbial community structure under different conditions was characterized by high-throughput sequencing, showing that <i>Firmicutes, Bacteroidetes</i>, <i>Chloroflexi</i>, <i>Synergistetes</i>, and <i>Proteobacteria</i> dominated the bacterial community, and that <i>Firmicutes</i> had a competitive advantage over <i>Bacteroidetes</i> at elevated temperatures. The biogas production values and relative abundance of <i>OPB54</i> and <i>Bacteroidia</i> after 20 d of SS-AD can be fitted well using a quadratic model, implying that <i>OPB54</i> and <i>Bacteroidia</i> play important roles in the methanogenic metabolism for agricultural waste thermophilic SS-AD.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38629858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of a Newly Isolated Chitinase-Producing Strain <i>Bacillus licheniformis</i> SSCL-10 for Chitin Degradation.","authors":"Abirami Sasi,&nbsp;Nagarajan Duraipandiyan,&nbsp;Kannan Marikani,&nbsp;Sugapriya Dhanasekaran,&nbsp;Noura Al-Dayan,&nbsp;Divya Venugopal","doi":"10.1155/2020/8844811","DOIUrl":"https://doi.org/10.1155/2020/8844811","url":null,"abstract":"<p><p>Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel <i>Bacillus licheniformis</i>. A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV. There were 16 mutant strains exhibiting chitinase activity. Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as <i>Bacillus licheniformis</i> (SSCL-10) with the highest specific activity of 3.4 U/mL. The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain. The partially purified chitinase has a molecular weight of 66 kDa. The wild strain (SSCL-10) was identified as <i>Bacillus licheniformis</i> using 16S rRNA sequence analysis. This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8844811","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38629859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Current Sensing Biosensor for BOD Rapid Measurement.","authors":"Yiman Liu,&nbsp;Jie Li,&nbsp;Nianxin Wan,&nbsp;Tianyu Fu,&nbsp;Lili Wang,&nbsp;Cong Li,&nbsp;Zhonghui Qie,&nbsp;Ao Zhu","doi":"10.1155/2020/8894925","DOIUrl":"https://doi.org/10.1155/2020/8894925","url":null,"abstract":"<p><p>In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, <i>Bacillus subtilis</i> was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms, which could increase the content of microorganisms and prolong the service life of the biological element. The conductivity (COND) probe is used as a sensing element, so that the testing value can be read every second. In the program, the moving average method is used to process the collected data so that the value can be read every minute. National standard samples were detected to test the accuracy and stability of the method. The results showed that relative error and analytical standard deviations were less than 5%. Different polluted water was tested to evaluate its application range. The results showed that relative error was less than 5%. The results of the method are consistent with the results of the wastewater sample obtained by the BOD₅ standard method. The proposed rapid BOD current sensing biosensor method should be promising in practical application of wastewater monitoring.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8894925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38605151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions <i>In Vitro</i>.","authors":"Mubarik Mahmood,&nbsp;Ratchaneewan Khiaosa-Ard,&nbsp;Qendrim Zebeli,&nbsp;Renée M Petri","doi":"10.1155/2020/8875773","DOIUrl":"https://doi.org/10.1155/2020/8875773","url":null,"abstract":"<p><p>Rumen archaea play an important role in scavenging ruminal hydrogen (H₂) and thus facilitate rumen fermentation. They require optimum temperature and osmolality for their growth and metabolism; however, a number of external factors may put archaea under heat and osmotic stress. Betaine is an osmolyte, molecular chaperone, and antioxidant; therefore, it bears potential to combat against these stressors. In this <i>in vitro</i> study, three betaine levels, namely, 0 (control), 51 (low), and 286 (high) ppm, were used. Each of these was subjected to two temperatures (39.5 and 42°C) and two osmolality conditions (295 and 420 mOsmol kg^-1) with <i>n</i> = 6 per treatment. Sequencing analyses of the solid phase (which use solid materials containing primarily fibrous materials of low-density feed particles) and the liquid phase (rumen fermenter liquid) using 16S rRNA revealed that more than 99.8% of the ruminal archaea in fermenters belong to the phylum <i>Euryarchaeota</i>. At the genus level, <i>Methanobrevibacter</i> was the most prevalent in both phases, and <i>Methanosaeta</i> was only detected in the liquid phase. The genera <i>Methanobrevibacter</i> and <i>Methanobacterium</i> both showed a positive correlation with methane (CH₄) formation in the liquid and solid phases, respectively (<i>P</i> < 0.05). Heat stress increased the relative abundance of genus <i>Methanimicrococcus</i> at the expense of candidate archaeal genus Vadin CA11 (<i>P</i> < 0.05). In the solid phase, osmotic stress significantly reduced the Shannon and Simpson indices of diversity, and relative abundance was higher for <i>Methanobrevibacter</i> at the expense of <i>Methanimicrococcus</i>. In the liquid phase, osmotic stress increased not only the abundance-based coverage estimator (ACE) and singles parameters of diversity but also the relative abundances of <i>Methanosphaera</i> and <i>Methanobacterium</i>. The overall decrease in all gas parameters and estimated metabolic hydrogen ([2H]) utilization was observed during osmotic stress conditions (<i>P</i> < 0.05). Betaine enhanced the diversity of solid phase archaea as indicated by the increase in ACE and singles during heat stress, and only a high dose improved all diversity parameters in the liquid phase during osmotic stress (<i>P</i> < 0.05). Thus, betaine alleviates the effects of heat stress and osmotic stress on the archaea community.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8875773","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38562818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Sludge Retention Time on the Performance of Anaerobic Ceramic Membrane Bioreactor Treating High-Strength Phenol Wastewater.","authors":"Chunhua He,&nbsp;Chuanhe Yang,&nbsp;Shoujun Yuan,&nbsp;Zhenhu Hu,&nbsp;Wei Wang","doi":"10.1155/2020/8895321","DOIUrl":"https://doi.org/10.1155/2020/8895321","url":null,"abstract":"<p><p>Anaerobic ceramic membrane bioreactor (AnCMBR) is an attractive alternative for the treatment of high-strength phenol wastewater, but the effects of sludge retention time (SRT) on the performance and membrane fouling are still unclear. The results indicated that the AnCMBR was successfully employed to treat high-strength wastewater containing 5 g phenol L^-1. The removal efficiencies of phenol and chemical oxygen demand (COD) reached over 99.5% and 99%, respectively, with long SRT and short SRT. SRT had no obvious effect on the performance of the AnCMBR treating high-strength phenol wastewater with long time operation. The strong performance robustness of AnCMBR benefited from the enrichment of hydrogenotrophic methanogens and syntrophic phenol-degrading bacteria. However, the decline of SRT led to a more severe membrane fouling in the AnCMBR, which was caused by the small size of sludge flocs and high concentration of protein in the biopolymers. Therefore, this work presented a comprehensive insight to the feasibility and robustness of the AnCMBR for treating high-strength phenol wastewater.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8895321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38295273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation of a Novel Tubular Microalgae Photobioreactor with Aerated Tangent Inner Tubes: Improvements in Mixing Performance and Flashing-Light Effects.","authors":"Xuyang Cui,&nbsp;Junhong Yang,&nbsp;Yuanzheng Feng,&nbsp;Wenwen Zhang","doi":"10.1155/2020/8815263","DOIUrl":"https://doi.org/10.1155/2020/8815263","url":null,"abstract":"<p><p>At present, large-scale and high-efficiency microalgal cultivation is the key to realizing the technology for carbon capture and storage (CCS) and bioresource recovery. Meanwhile, tubular photobioreactors (PBRs) have great potential for microalgal cultivation due to their high productivity. To improve the mixing performance and flashing-light effect, a novel tube PBR with the inner tube tangential to the outer tube was developed, whose radial aeration pores are situated along the length of the inner tube. The direction of aeration, aeration rate, light/dark cycle period (L/D), light-time ratio, average turbulent kinetic energy (TKE), and degree of synergy between the velocity and direction of the light field in the PBR were optimized by a computational fluid dynamics (CFD) simulation and field synergy theory. The results show that a downwards aeration direction of 30° and an aeration rate of 0.7 vvm are the most conducive to reducing the dead zone and improving the light/dark cycle frequency. Compared to the concentric double-tube PBR, the light/dark cycle frequency and light time of the tangent double-tube PBR increased by 78.2% and 36.2% to 1.8 Hz and 47.8%, respectively, and the TKE was enhanced by 48.1% from 54 to 80 cm²·s^-2. Meanwhile, field synergy theory can be extended and applied to the design of tubular microalgae PBRs, and the average synergy of the light and velocity gradients across the cross-section increased by 38% to 0.69. The tangential inner tube aeration structure generated symmetrical vertical vortices between the light and dark areas in the PBR, which significantly improved the mixing performance and flashing-light effect. This novel design can provide a more suitable microenvironment for microalgal cultivation and is promising for bioresource recovery applications and improving the yield of microalgae.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8815263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38234665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response and Adaptation of Microbial Community in a CANON Reactor Exposed to an Extreme Alkaline Shock.","authors":"Ruili Yang,&nbsp;Wenlong Mao,&nbsp;Xiaojun Wang,&nbsp;Zhaoji Zhang,&nbsp;Junbin Wu,&nbsp;Shaohua Chen","doi":"10.1155/2020/8888615","DOIUrl":"https://doi.org/10.1155/2020/8888615","url":null,"abstract":"<p><p>Responses of a microbial community in the completely autotrophic nitrogen removal over nitrite (CANON) process, which was shocked by a pH of 11.0 for 12 h, were investigated. During the recovery phase, the performance, anaerobic ammonia oxidation (anammox) activity, microbial community, and correlation of bacteria as well as the influencing factors were evaluated synchronously. The performance of the CANON process deteriorated rapidly with a nitrogen removal rate (NRR) of 0.13 kg·m^-3·d^-1, and Firmicutes, spore-forming bacteria, were the dominant phyla after alkaline shock. However, it could self-restore within 107 days after undergoing four stages, at which Planctomycetes became dominant with a relative abundance of 64.62%. Network analysis showed that anammox bacteria (<i>Candidatus Jettenia</i>, <i>Kuenenia</i>, and <i>Brocadia</i>) were positively related to some functional bacteria such as <i>Nitrosomonas</i>, <i>SM1A02</i>, and <i>Calorithrix</i>. Canonical correspondence analysis presented a strong correlation between the microbial community and influencing factors during the recovery phase. With the increase of nitrogen loading rate, the decrease of free nitrous acid and the synergistic effects, heme c content, specific anammox activity (SAA), NRR, and the abundance of dominant genus increased correspondingly. The increase of heme c content regulates the quorum sensing system, promotes the secretion of extracellular polymeric substances, and further improves SAA, NRR, and the relative abundance of the dominant genus. This study highlights some implications for the recovery of the CANON reactor after being exposed to an alkaline shock.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8888615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosorption Mechanism of Aqueous Pb²⁺, Cd²⁺, and Ni²⁺ Ions on Extracellular Polymeric Substances (EPS).","authors":"Di Cui,&nbsp;Chong Tan,&nbsp;Hongna Deng,&nbsp;Xunxue Gu,&nbsp;Shanshan Pi,&nbsp;Ting Chen,&nbsp;Lu Zhou,&nbsp;Ang Li","doi":"10.1155/2020/8891543","DOIUrl":"https://doi.org/10.1155/2020/8891543","url":null,"abstract":"<p><p>Heavy metal pollution has been a focus with increasing attention, especially Pb²⁺, Cd²⁺, and Ni²⁺ in an aqueous environment. The adsorption capacity and mechanism of extracellular polymeric substances (EPS) from <i>Agrobacterium tumefaciens</i> F2 for three heavy metals were investigated in this study. The adsorption efficiency of 94.67%, 94.41%, and 77.95% were achieved for Pb²⁺, Cd²⁺, and Ni²⁺ adsorption on EPS, respectively. The experimental data of adsorption could be well fitted by Langmuir, Freundlich, Dubinin-Radushkevich isotherm models, and pseudo-second-order kinetic model. Model parameters analysis demonstrated the great adsorption efficiency of EPS, especially for Pb²⁺, and chemisorption was the rate-limiting step during the adsorption process. The functional groups of C=O of carboxyl and C-O-C from sugar derivatives in EPS played the major role in the adsorption process judged by FTIR. In addition, 3D-EEM spectra indicated that tyrosine also assisted EPS adsorption for three heavy metals. But EPS from strain F2 used the almost identical adsorption mechanism for three kinds of divalent ions of heavy metals, so the adsorption efficiency difference of Pb²⁺, Cd²⁺, and Ni²⁺ on EPS could be correlated to the inherent characteristics of each heavy metal. This study gave the evidence that EPS has a great application potential as a bioadsorbent in the treatment of heavy metals pollution.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8891543","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of Marine Macroalgae (<i>Chaetomorpha maxima</i>) with a Moving Bed Bioreactor for Nutrient Removal from Maricultural Wastewater.","authors":"Xian Li,&nbsp;Yale Deng,&nbsp;Xueying Li,&nbsp;Xiaona Ma,&nbsp;Jinxia Wang,&nbsp;Jun Li","doi":"10.1155/2020/8848120","DOIUrl":"https://doi.org/10.1155/2020/8848120","url":null,"abstract":"<p><p>Rather than direct nutrient removal from wastewaters, an alternative approach aimed at nutrient recovery from aquacultural wastewaters could enable sustainable management for aquaculture production. This study demonstrated the feasibility of cultivating marine macroalgae (<i>Chaetomorpha maxima</i>) with a moving bed bioreactor (MBBR-MA), to remove nitrogen and phosphorus in aquaculture wastewater as well as to produce macroalgae biomass. MBBR-MA significantly increased the simultaneous removal of nitrate and phosphate in comparison with only MBBR, resulting in an average total nitrogen (TN) and total phosphorus (TP) removal efficiency of 42.8 ± 5.5% and 83.7 ± 7.7%, respectively, in MBBR-MA while MBBR had no capacity for TN and TP removal. No chemical oxygen demand (COD) removal was detected in both reactors. Phosphorus could be a limiting factor for nitrogen uptake when N : P ratio increased. The recovered nitrogen and phosphorus resulted in a specific growth rate of 3.86%-10.35%/day for <i>C. maxima</i> with an uptake N : P ratio of 6. The presence of macroalgae changed the microbial community in both the biofilter and water by decreasing the relative abundance of Proteobacteria and Nitrospirae and increasing the abundance of Bacteroidetes. These findings indicate that the integration of the macroalgae <i>C. maxima</i> with MBBR could represent an effective wastewater treatment option, especially for marine recirculating aquaculture systems.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8848120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Biohydrogen Production and Performance of Hydrogen-Producing Acetogen by Increasing Normal Molasses Wastewater Proportion in Anaerobic Baffled Reactor.","authors":"Xuejia Gu,&nbsp;Yufeng Wang,&nbsp;Huaibo Li,&nbsp;Ji Li,&nbsp;Shuo Wang","doi":"10.1155/2020/8885662","DOIUrl":"https://doi.org/10.1155/2020/8885662","url":null,"abstract":"<p><p>The biohydrogen production efficiency and performance of hydrogen-producing acetogen in a four-compartment anaerobic baffled reactor (ABR) were studied by gradually increasing the influent normal molasses wastewater (NMWW) proportion. When the influent NMWW proportion increased to 55%, ABR could develop microbial community with methanogenic function in 63 days and reach a stable operation. When the influent NMWW proportion increased to 80% and reached a stable state, ethanol fermentation was established from butyric acid fermentation in the first three compartments, whereas butyric acid fermentation in the fourth compartment was strengthened. The average biohydrogen production yield and biohydrogen production capacity by COD removal increased to as high as 12.85 L/day and 360.22 L/kg COD when the influent NMWW proportion increased from 55% to 80%, respectively. Although the biogas yield and the specific biogas production rate reached 61.54 L/day and 232 L/kg MLVSS·day, the biohydrogen production yield and specific biohydrogen production rate were only 12.85 L/day and 48 L/kg MLVSS·day, which results in hydrogen consumption by homoacetogenesis and methanogenesis.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2020-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8885662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38114027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}