Response and Adaptation of Microbial Community in a CANON Reactor Exposed to an Extreme Alkaline Shock.

IF 2.3 4区 生物学 Q3 MICROBIOLOGY
Archaea-An International Microbiological Journal Pub Date : 2020-06-23 eCollection Date: 2020-01-01 DOI:10.1155/2020/8888615
Ruili Yang, Wenlong Mao, Xiaojun Wang, Zhaoji Zhang, Junbin Wu, Shaohua Chen
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引用次数: 10

Abstract

Responses of a microbial community in the completely autotrophic nitrogen removal over nitrite (CANON) process, which was shocked by a pH of 11.0 for 12 h, were investigated. During the recovery phase, the performance, anaerobic ammonia oxidation (anammox) activity, microbial community, and correlation of bacteria as well as the influencing factors were evaluated synchronously. The performance of the CANON process deteriorated rapidly with a nitrogen removal rate (NRR) of 0.13 kg·m-3·d-1, and Firmicutes, spore-forming bacteria, were the dominant phyla after alkaline shock. However, it could self-restore within 107 days after undergoing four stages, at which Planctomycetes became dominant with a relative abundance of 64.62%. Network analysis showed that anammox bacteria (Candidatus Jettenia, Kuenenia, and Brocadia) were positively related to some functional bacteria such as Nitrosomonas, SM1A02, and Calorithrix. Canonical correspondence analysis presented a strong correlation between the microbial community and influencing factors during the recovery phase. With the increase of nitrogen loading rate, the decrease of free nitrous acid and the synergistic effects, heme c content, specific anammox activity (SAA), NRR, and the abundance of dominant genus increased correspondingly. The increase of heme c content regulates the quorum sensing system, promotes the secretion of extracellular polymeric substances, and further improves SAA, NRR, and the relative abundance of the dominant genus. This study highlights some implications for the recovery of the CANON reactor after being exposed to an alkaline shock.

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CANON反应器中微生物群落对极端碱性冲击的响应与适应
研究了在pH为11.0的条件下,一个微生物群落在完全自养亚硝酸盐脱氮(CANON)过程中对12 h的反应。在回收阶段,同步评价其性能、厌氧氨氧化(anammox)活性、微生物群落、细菌相关性及影响因素。碱性冲击后,CANON工艺性能迅速恶化,氮去除率(NRR)为0.13 kg·m-3·d-1,产孢菌厚壁菌门为优势菌门。但经过4个阶段后,107 d内可自我恢复,在此阶段plantomycetes成为优势菌,相对丰度为64.62%。网络分析显示厌氧氨氧化菌(Candidatus Jettenia、Kuenenia和Brocadia)与部分功能菌如Nitrosomonas、SM1A02和Calorithrix呈正相关。典型对应分析表明,在恢复阶段,微生物群落与影响因素之间存在较强的相关性。随着氮负荷速率的增加、游离亚硝酸盐的减少和协同效应的增强,血红素c含量、特定厌氧氨氧化活性(SAA)、NRR和优势属丰度相应增加。血红素c含量的增加调节了群体感应系统,促进了胞外聚合物质的分泌,进一步提高了优势属的SAA、NRR和相对丰度。本研究强调了CANON反应器暴露于碱性冲击后恢复的一些含义。
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来源期刊
CiteScore
7.50
自引率
0.00%
发文量
1
审稿时长
>12 weeks
期刊介绍: Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.
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