Lianghu Su, Xu Sun, Chenwei Liu, Rongting Ji, Guangyin Zhen, Mei Chen, Longjiang Zhang
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Among the three operation parameters, the C/N ratio had the largest effect on biogas production, followed by temperature, and a maximum biogas yield of 241.4 mL gVS<sup>-1</sup> could be achieved at 47.3°C, ISC = 24.81%, and C/N = 22.35. After 20 d of SS-AD, the microbial community structure under different conditions was characterized by high-throughput sequencing, showing that <i>Firmicutes, Bacteroidetes</i>, <i>Chloroflexi</i>, <i>Synergistetes</i>, and <i>Proteobacteria</i> dominated the bacterial community, and that <i>Firmicutes</i> had a competitive advantage over <i>Bacteroidetes</i> at elevated temperatures. 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引用次数: 0
摘要
本研究对农业废弃物(即玉米秸秆、牛粪和蔬菜废弃物)进行了嗜热固态厌氧消化(SS-AD)。采用箱-贝肯实验设计(BBD)结合响应面方法(RSM),评估了温度(40-60°C)、初始固体含量(ISC,17.5-32.5%)和碳/氮比(15-32:1)对沼气产量的影响。结果表明,工艺参数的优化对提高 SS-AD 的性能非常重要。在使用 SS-AD 生产沼气的二次方模型中,包括交互项(ISC 除外)在内的所有因素都很重要。在三个运行参数中,C/N 比对沼气产量的影响最大,其次是温度,在 47.3°C、ISC = 24.81%、C/N = 22.35 时,沼气产量最大,为 241.4 mL gVS-1。在 SS-AD 20 d 后,通过高通量测序对不同条件下的微生物群落结构进行了表征,结果表明,固氮菌、类杆菌、绿僵菌、协同菌和变形菌在细菌群落中占主导地位,在高温条件下,固氮菌比类杆菌更具竞争优势。使用二次模型可以很好地拟合嗜热 SS-AD 20 d 后 OPB54 和类杆菌的沼气产值和相对丰度,这意味着 OPB54 和类杆菌在农业废弃物嗜热 SS-AD 的产甲烷代谢过程中发挥了重要作用。
Thermophilic Solid-State Anaerobic Digestion of Corn Straw, Cattle Manure, and Vegetable Waste: Effect of Temperature, Total Solid Content, and C/N Ratio.
Thermophilic solid-state anaerobic digestion (SS-AD) of agricultural wastes, i.e., corn straw, cattle manure, and vegetable waste, was carried out in this study. The effects of temperature (40-60°C), initial solid content (ISC, 17.5-32.5%), and C/N ratio (15-32 : 1) on biogas production were evaluated using a Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). The results showed that optimization of process parameters is important to promote the SS-AD performance. All the factors, including interactive terms (except the ISC), were significant in the quadratic model for biogas production with SS-AD. Among the three operation parameters, the C/N ratio had the largest effect on biogas production, followed by temperature, and a maximum biogas yield of 241.4 mL gVS-1 could be achieved at 47.3°C, ISC = 24.81%, and C/N = 22.35. After 20 d of SS-AD, the microbial community structure under different conditions was characterized by high-throughput sequencing, showing that Firmicutes, Bacteroidetes, Chloroflexi, Synergistetes, and Proteobacteria dominated the bacterial community, and that Firmicutes had a competitive advantage over Bacteroidetes at elevated temperatures. The biogas production values and relative abundance of OPB54 and Bacteroidia after 20 d of SS-AD can be fitted well using a quadratic model, implying that OPB54 and Bacteroidia play important roles in the methanogenic metabolism for agricultural waste thermophilic SS-AD.
期刊介绍:
Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.