Cell and Bioscience最新文献_第7页

Mechanism and application of lactylation in cancers. 乳酸化在癌症中的作用机制及应用。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-06-04 DOI: 10.1186/s13578-025-01415-9

Jiewen Wang, Mingjing Peng, Linda Oyang, Mengzhou Shen, Shizhen Li, Xianjie Jiang, Zongyao Ren, Qiu Peng, Xuemeng Xu, Shiming Tan, Longzheng Xia, Wenjuan Yang, Haofan Li, Nayiyuan Wu, Yanyan Tang, Jinguan Lin, Qianjin Liao, Yaqian Han, Yujuan Zhou

引用次数: 0

Linear ubiquitination of p31^comet by HOIP couples cytokine response with mitotic regulation. HOIP对p31comet的线性泛素化将细胞因子反应与有丝分裂调节结合起来。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-06-03 DOI: 10.1186/s13578-025-01416-8

Yifeng Gao, Qing Yin, Yaser Gamallat, Michael G Grant, Aidan H Snell, Xingxing Shi, Lara N Ulstad, Arshita Singh, Tingan Chen, Joseph O Johnson, Dorina Avram, Lixin Wan

{"title":"Linear ubiquitination of p31comet by HOIP couples cytokine response with mitotic regulation.","authors":"Yifeng Gao, Qing Yin, Yaser Gamallat, Michael G Grant, Aidan H Snell, Xingxing Shi, Lara N Ulstad, Arshita Singh, Tingan Chen, Joseph O Johnson, Dorina Avram, Lixin Wan","doi":"10.1186/s13578-025-01416-8","DOIUrl":"10.1186/s13578-025-01416-8","url":null,"abstract":"Background: Inflammation and genomic instability are among the hallmarks of human cancer. Proinflammatory cytokines induce DNA damage through the accumulation of reactive oxygen and nitrogen species (RONS), which often leads to base alternations. The link between proinflammatory cytokines and chromosomal instability remains largely elusive.Results: Here, we report that the mitotic checkpoint protein p31comet (MAD2L1BP) is modified by linear ubiquitination via the E3 ubiquitin ligase HOIP after cytokine stimulation. HOIP-mediated polyubiquitination of p31comet occurs on its C-terminal lysine residues. Ubiquitinated p31comet displays reduced binding to PLK1, which phosphorylates and inactivates p31comet. Thus HOIP positively regulates p31comet function. Consistent with this notion, HOIP-deficient cells exhibit prolonged mitotic duration similar to p31comet knockout. Mitotic defects are also more prevalent in cells without HOIP or p31comet. Moreover, compared with the cells expressing wild-type p31comet, cells expressing a ubiquitination-deficient p31comet mutant take more time to complete the M phase.Conclusions: Our results together uncover a mechanistic link between the proinflammatory cytokines and the mitotic checkpoint pathways. This molecular switch could be explored as a potential therapeutic target in inflammation-driving or p31comet overexpressed cancer types.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"75"},"PeriodicalIF":6.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12135451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel roles for CREG1 in hematopoiesis revealed by single-cell RNA sequencing. 单细胞RNA测序揭示了CREG1在造血中的新作用。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-06-01 DOI: 10.1186/s13578-025-01407-9

Xiao Han, Keliang Pang, Xiaohui Liu, Jun Zhou, Jun Zhu, Hao Yuan

{"title":"Novel roles for CREG1 in hematopoiesis revealed by single-cell RNA sequencing.","authors":"Xiao Han, Keliang Pang, Xiaohui Liu, Jun Zhou, Jun Zhu, Hao Yuan","doi":"10.1186/s13578-025-01407-9","DOIUrl":"10.1186/s13578-025-01407-9","url":null,"abstract":"Background: Hematopoiesis, the process of generating diverse blood cell lineages, is essential for maintaining organismal homeostasis and survival. CREG1 has been implicated in various cellular processes, including proliferation, differentiation, and senescence. However, its role in adult hematopoiesis and the development of specific blood cell lineages remains largely unknown.Results: In this study, we utilized single-cell RNA sequencing (scRNA-seq) to investigate the function of CREG1 in hematopoietic development in adult zebrafish. Our analysis revealed significant alterations in cellular composition and gene expression profiles in kidney marrow of creg1-deficient zebrafish, particularly affecting B cell, T/NK cell, and erythroid cell development. The loss of CREG1 led to impaired endocytosis and lysosomal activity in lymphocytes, and inhibited differentiation of classical erythroid cells while promoting the development of immune-associated erythroid cells. These findings highlight a critical role for CREG1 in regulating hematopoietic lineage development, potentially through modulation of cell survival, endocytosis, and lysosomal function.Conclusions: Our study expands the current understanding of CREG1's role in hematopoiesis and provides a foundation for future investigations into the specific molecular mechanisms by which CREG1 regulates hematopoietic lineage development and function.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"74"},"PeriodicalIF":6.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency. 化学定义和无xeno培养基能够从丢弃的囊胚中高效地衍生出人类扩展多能干细胞系。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-30 DOI: 10.1186/s13578-025-01410-0

Zhuran Zhao, Xi Chen, Shan Wang, Min Fu, Huan Shen, Jiayu Li, Jun Xu, Jiong Qin, Cheng Shi

引用次数: 0

Hippocampal CA1 neuron, a crucial regulator for chronic stress exacerbating Alzheimer's disease progression. 海马CA1神经元，慢性应激加剧阿尔茨海默病进展的关键调节因子。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-30 DOI: 10.1186/s13578-025-01420-y

Qing-Lin Gao, Hai-Wei Zha, Zi-Jie Liu, Miao-Miao Wang, Yu-Qing Zhang, Jia-Rui Bi, Tian-Yang Wu, Zhen-Jiang Liu, Hui Wu, Dong Sun

{"title":"Hippocampal CA1 neuron, a crucial regulator for chronic stress exacerbating Alzheimer's disease progression.","authors":"Qing-Lin Gao, Hai-Wei Zha, Zi-Jie Liu, Miao-Miao Wang, Yu-Qing Zhang, Jia-Rui Bi, Tian-Yang Wu, Zhen-Jiang Liu, Hui Wu, Dong Sun","doi":"10.1186/s13578-025-01420-y","DOIUrl":"10.1186/s13578-025-01420-y","url":null,"abstract":"Chronic stress, a common risk factor for psychiatric disorders, is also implicated in the pathogenesis of Alzheimer's disease (AD). However, its underlying mechanisms remain elusive. Here, we provide evidence for chronic restraint stress (CRS), a widely used stress model in rodents, to regulate AD pathology. CRS not only induces prolonged depressive-like behaviors and cognitive deficits in young adult wild type (WT) mice, but also exacerbates a series of AD-related phenotypes in APP/PS1 mice, including impaired spatial learning and memory, increased β-amyloid plaques, promoted glial cells (astrocyte and microglial cell) activation and decreased dendritic spines in CA1 neurons. Single-nucleus RNA-sequencing analysis in hippocampus shows remarkable transcriptional changes in many cell type(s), and identifies oxidative phosphorylation pathway, a major source for adenosine triphosphate (ATP) production, is significantly downregulated in CA1 neurons by CRS stimuli. Furthermore, dysfunctional mitochondria and reduced ATP levels are also observed in CA1 neurons of CRS exposed WT and APP/PS1 mice. Interestingly, infusion of ATP in CA1 region abolishes the deficits in cognition, dendritic spines and glial activation in CRS exposed APP/PS1 mice. Taken together, these results uncover an unrecognized function of CA1 neurons in regulating CRS induced AD pathologies, and suggest ATP as a promising therapeutic strategy to improve brain health under stress condition.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"73"},"PeriodicalIF":6.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shedding light on the cell biology and diverse physiological functions of the migrasome. 揭示迁移体的细胞生物学和多种生理功能。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-28 DOI: 10.1186/s13578-025-01417-7

Yuxing Huang, Yi Huang, Jian Gao

引用次数: 0

Emerging roles of ribosome translation in stem cells and stem cell therapy - a review. 核糖体翻译在干细胞和干细胞治疗中的新作用综述。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-28 DOI: 10.1186/s13578-025-01412-y

Yanyan Gao, Linlin Guo, Gaoxiang Shi, Ruifang Wang, Xu'an Wang, Jizhong Lou

引用次数: 0

Rab11a-dependent recycling of Glut3 inhibits seizure-induced neuronal disulfidptosis by alleviating glucose deficiency. 依赖rab11a的Glut3循环通过减轻葡萄糖缺乏抑制癫痫诱导的神经元二亢。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-28 DOI: 10.1186/s13578-025-01396-9

Sijun Li, Junrui He, Huimin Kuang, Xiaojuan Wang, Muhua Zhou, Dongmei Li, Baoren Kang, Honghu He, Lina He, Wei Lin, Yuan Lv

{"title":"Rab11a-dependent recycling of Glut3 inhibits seizure-induced neuronal disulfidptosis by alleviating glucose deficiency.","authors":"Sijun Li, Junrui He, Huimin Kuang, Xiaojuan Wang, Muhua Zhou, Dongmei Li, Baoren Kang, Honghu He, Lina He, Wei Lin, Yuan Lv","doi":"10.1186/s13578-025-01396-9","DOIUrl":"10.1186/s13578-025-01396-9","url":null,"abstract":"Seizures can trigger neuronal glucose deficiency, thereby inducing disulfidptosis. Disulfidptosis is a novel cell death mechanism characterized by the abnormal accumulation of disulfide caused by glucose deficiency. However, the mechanism underlying disulfidptosis caused by glucose deficiency in seizures remains elusive. Rab11a-dependent recycling of glucose transporter 3 (Glut3) is closely related to glucose metabolism in neurons, which may contribute to neuronal disulfidptosis after seizures by abnormal glucose metabolism. So here we introduced a well-established in vitro model of seizures to evaluate cell survival, glucose levels, disulfidptosis biomarkers, Glut3 and Rab11a expression, the recycling ratio of Glut3, and the protein complex of Glut3-Rab11a. Cell survival rates and glucose levels were lower in the in vitro model of seizures, accompanied by elevated levels of disulfidptosis markers. Moreover, the surface expression and the recycling ratio of Glut3, as well as the protein complex of Glut3-Rab11a, were positively correlated with Rab11a expression. Lastly, Rab11 overexpression improved cell survival rates, increased glucose levels, and decreased the levels of disulfidptosis biomarkers in the in vitro model of seizure. Rab11a-dependent recycling of Glut3 inhibited seizure-induced neuronal disulfidptosis by alleviating glucose deficiency.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"69"},"PeriodicalIF":6.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of the critical role of the epithelial mesenchymal transition subtype - TAGLN-positive fibroblasts in colorectal cancer progression and immunosuppression. 综合分析上皮间充质转化亚型- tagln阳性成纤维细胞在结直肠癌进展和免疫抑制中的关键作用。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-24 DOI: 10.1186/s13578-025-01405-x

Junli Zhang, Xinxin Jin, Yachao Hou, Biao Gu, Hongwei Li, Li Yi, Wenjuan Wu, Shangshang Hu

{"title":"Comprehensive analysis of the critical role of the epithelial mesenchymal transition subtype - TAGLN-positive fibroblasts in colorectal cancer progression and immunosuppression.","authors":"Junli Zhang, Xinxin Jin, Yachao Hou, Biao Gu, Hongwei Li, Li Yi, Wenjuan Wu, Shangshang Hu","doi":"10.1186/s13578-025-01405-x","DOIUrl":"10.1186/s13578-025-01405-x","url":null,"abstract":"Epithelial-mesenchymal transition (EMT) plays a pivotal role in tumor metastasis and immune suppression in colorectal cancer (CRC). However, the specific mechanisms of EMT and its relationship with the clinical prognosis and immunotherapy response in CRC patients remain unclear. In this study, we identified TAGLN-positive fibroblasts (TAGLN⁺Fib) as a cancer-associated fibroblast (CAF) subtype within the tumor microenvironment (TME) that promotes tumor metastasis and immune evasion. High EMT scores, strongly associated with TAGLN expression, were correlated with advanced tumor stages, poor prognosis, and resistance to immunotherapy. Functional experiments demonstrated that TAGLN knockdown significantly reduced CRC cell proliferation, migration, and EMT phenotypes in vitro and suppressed tumor growth in vivo. Furthermore, TAGLN⁺Fib closely interacted with MMP7-positive tumor epithelial cells and SPP1-positive macrophages, forming a pro-metastatic and immunosuppressive network. An EMT-TME risk model constructed using TAGLN⁺Fib exhibited robust predictive power for CRC prognosis and immunotherapy response. This study reveals the association of EMT scores with CRC prognosis and immunotherapy response, highlights TAGLN⁺Fib's critical role in tumor progression, and develops an EMT-TME risk model, offering insights for personalized CRC treatment and precision medicine.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"66"},"PeriodicalIF":6.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia-ischemia and sexual dimorphism: modeling mitochondrial dysfunction using brain organoids. 缺氧缺血和两性二态性：用脑类器官模拟线粒体功能障碍。

IF 6.1 2区生物学

Cell and Bioscience Pub Date : 2025-05-24 DOI: 10.1186/s13578-025-01402-0

Romane Gaston-Breton, Clémence Disdier, Henrik Hagberg, Aloïse Mabondzo

{"title":"Hypoxia-ischemia and sexual dimorphism: modeling mitochondrial dysfunction using brain organoids.","authors":"Romane Gaston-Breton, Clémence Disdier, Henrik Hagberg, Aloïse Mabondzo","doi":"10.1186/s13578-025-01402-0","DOIUrl":"10.1186/s13578-025-01402-0","url":null,"abstract":"Hypoxic-ischemic encephalopathy (HIE) is a leading cause of neurodevelopmental morbidities in full-term infants. There is strong evidence of sexual differences in hypoxic-ischemic (HI) injury where male neonates are at higher risk as they are subject to more pronounced neurological deficits and death than females. The cellular and molecular mechanisms underlying these sexual discrepancies in HI injury are poorly understood. Mitochondrial dysregulation has been increasingly explored in brain diseases and represents a major target during HI events. In this review, we discuss (1) different mitochondrial functions in the central nervous system (2), mitochondrial dysregulation in the context of HI injury (3), sex-dependent mitochondrial pathways in HIE and (4) modeling of mitochondrial dysfunction using human brain organoids. Gaining insight into these novel aspects of mitochondrial function will offer valuable understanding of brain development and neurological disorders such as HI injury, paving the way for the discovery and creation of new treatment approaches.","PeriodicalId":49095,"journal":{"name":"Cell and Bioscience","volume":"15 1","pages":"67"},"PeriodicalIF":6.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0