Human Vaccines & Immunotherapeutics最新文献

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Formulation development of a stable influenza recombinant neuraminidase vaccine candidate. 稳定型流感重组神经氨酸酶候选疫苗的配方开发。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-03-18 DOI: 10.1080/21645515.2024.2304393
Bing Li, Irina V Ustyugova, Lisa Szymkowicz, Shaolong Zhu, Marin Ming, Karen Y Y Fung, Guadalupe Cortés, D Andrew James, Michael Hrynyk, Nausheen Rahman, Roger H Brookes, Salvador F Ausar
{"title":"Formulation development of a stable influenza recombinant neuraminidase vaccine candidate.","authors":"Bing Li, Irina V Ustyugova, Lisa Szymkowicz, Shaolong Zhu, Marin Ming, Karen Y Y Fung, Guadalupe Cortés, D Andrew James, Michael Hrynyk, Nausheen Rahman, Roger H Brookes, Salvador F Ausar","doi":"10.1080/21645515.2024.2304393","DOIUrl":"10.1080/21645515.2024.2304393","url":null,"abstract":"<p><p>Current influenza vaccines could be augmented by including recombinant neuraminidase (rNA) protein antigen to broaden protective immunity and improve efficacy. Toward this goal, we investigated formulation conditions to optimize rNA physicochemical stability. When rNA in sodium phosphate saline buffer (NaPBS) was frozen and thawed (F/T), the tetrameric structure transitioned from a \"closed\" to an \"open\" conformation, negatively impacting functional activity. Hydrogen deuterium exchange experiments identified differences in anchorage binding sites at the base of the open tetramer, offering a structural mechanistic explanation for the change in conformation and decreased functional activity. Change to the open configuration was triggered by the combined stresses of acidic pH and F/T. The desired closed conformation was preserved in a potassium phosphate buffer (KP), minimizing pH drop upon freezing and including 10% sucrose to control F/T stress. Stability was further evaluated in thermal stress studies where changes in conformation were readily detected by ELISA and size exclusion chromatography (SEC). Both tests were suitable indicators of stability and antigenicity and considered potential critical quality attributes (pCQAs). To understand longer-term stability, the pCQA profiles from thermally stressed rNA at 6 months were modeled to predict stability of at least 24-months at 5°C storage. In summary, a desired rNA closed tetramer was maintained by formulation selection and monitoring of pCQAs to produce a stable rNA vaccine candidate. The study highlights the importance of understanding and controlling vaccine protein structural and functional integrity.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10950269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human papillomavirus vaccination uptake and determinant factors among adolescent schoolgirls in sub-Saharan Africa: A systematic review and meta-analysis. 撒哈拉以南非洲女学生接种人类乳头瘤病毒疫苗的情况及其决定因素:系统回顾和荟萃分析。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-03-20 DOI: 10.1080/21645515.2024.2326295
Yordanos Sisay Asgedom, Tsegaye Melaku Kebede, Beminate Lemma Seifu, Kusse Urmale Mare, Zufan Alamrie Asmare, Hiwot Altaye Asebe, Bizunesh Fantahun Kase, Abdu Hailu Shibeshi, Tsion Mulat Tebeje, Kebede Gemeda Sabo, Bezawit Melak Fente, Afework Alemu Lombebo, Mengistu Meskele Koyira, Gizachew Ambaw Kassie
{"title":"Human papillomavirus vaccination uptake and determinant factors among adolescent schoolgirls in sub-Saharan Africa: A systematic review and meta-analysis.","authors":"Yordanos Sisay Asgedom, Tsegaye Melaku Kebede, Beminate Lemma Seifu, Kusse Urmale Mare, Zufan Alamrie Asmare, Hiwot Altaye Asebe, Bizunesh Fantahun Kase, Abdu Hailu Shibeshi, Tsion Mulat Tebeje, Kebede Gemeda Sabo, Bezawit Melak Fente, Afework Alemu Lombebo, Mengistu Meskele Koyira, Gizachew Ambaw Kassie","doi":"10.1080/21645515.2024.2326295","DOIUrl":"10.1080/21645515.2024.2326295","url":null,"abstract":"<p><p>Despite the ongoing global vaccination campaign aimed at preventing human papillomavirus (HPV) related health issues, the uptake of the HPV vaccine remains unacceptably low in developing regions, particularly in sub-Saharan Africa (SSA). Therefore, this systematic review and meta-analysis aimed at determining the pooled prevalence and associated factors of HPV vaccine uptake among adolescent school girls in SSA. Electronic bio-medical databases were explored. Pooled prevalence, publication bias, meta-regression, sub-group, and sensitivity analysis were performed. The estimated pooled prevalence of HPV vaccine uptake was 28.53% [95% CI: (5.25, 51.81)]. Having good knowledge and a positive attitude was significantly associated with HPV vaccine uptake in SSA. Subgroup analysis revealed the highest uptake was 62.52% from Kenya and the lowest was 3.77% in Nigeria. The HPV vaccine uptake is low. It underscores the need for community education, school-based immunization, and education programs that promote the uptake of the vaccine to increase coverage.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of COVID-19 vaccination status on hospitalization and disease severity: A descriptive study in Nagasaki Prefecture, Japan. COVID-19 疫苗接种情况对住院和疾病严重程度的影响:日本长崎县的一项描述性研究。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-03-22 DOI: 10.1080/21645515.2024.2322795
Guoxi Cai, Shiwen Liu, Yixiao Lu, Yumika Takaki, Fumiaki Matsumoto, Akira Yoshikawa, Toshitsugu Taguri, Jianfen Xie, Kazuhiko Arima, Satoshi Mizukami, Jiwen Wu, Taro Yamamoto, Maiko Hasegawa, Nguyen Tien Huy, Masaya Saito, Shouhei Takeuchi, Kouichi Morita, Kiyoshi Aoyagi, Fei He
{"title":"Impact of COVID-19 vaccination status on hospitalization and disease severity: A descriptive study in Nagasaki Prefecture, Japan.","authors":"Guoxi Cai, Shiwen Liu, Yixiao Lu, Yumika Takaki, Fumiaki Matsumoto, Akira Yoshikawa, Toshitsugu Taguri, Jianfen Xie, Kazuhiko Arima, Satoshi Mizukami, Jiwen Wu, Taro Yamamoto, Maiko Hasegawa, Nguyen Tien Huy, Masaya Saito, Shouhei Takeuchi, Kouichi Morita, Kiyoshi Aoyagi, Fei He","doi":"10.1080/21645515.2024.2322795","DOIUrl":"10.1080/21645515.2024.2322795","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19) was extraordinarily harmful, with high rates of infection and hospitalization. This study aimed to evaluate the impact of COVID-19 vaccination status and other factors on hospitalization and disease severity, using data from Nagasaki Prefecture, Japan. Confirmed cases of COVID-19 infection with vaccination status were included and the differences in characteristics between different vaccination statuses, hospitalization or not, and patients with varying levels of disease severity were analyzed. Furthermore, logistic regression was used to calculate odds ratio (ORs) and 95% confidence intervals (CI) to evaluate the association of various factors with hospitalization and disease severity. From March 14, 2020 to August 31, 2022, 23,139 patients were unvaccinated 13,668 vaccinated the primary program with one or two doses, and 4,575 completed the booster. Vaccination reduced the risk of hospitalization with an odd ratio of 0.759 (95% CI: 0.654-0.881) and the protective effect of completed booster vaccination was more pronounced (OR: 0.261, 95% CI: 0.207-0.328). Similarly, vaccination significantly reduced the risk of disease severity (vaccinated primary program: OR: 0.191, 95% CI: 0.160-0.228; completed booster vaccination: OR: 0.129, 95% CI: 0.099-0.169). Overall, unvaccinated, male, elderly, immunocompromised, obese, and patients with other severe illness factors were all risk factors for COVID-19-related hospitalization and disease severity. Vaccination was associated with a decreased risk of hospitalization and disease severity, and highlighted the benefits of completing booster.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Opportunities for HPV vaccine education in school-based immunization programs in British Columbia, Canada: A qualitative study. 加拿大不列颠哥伦比亚省校本免疫计划中的 HPV 疫苗教育机会:定性研究。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-03-22 DOI: 10.1080/21645515.2024.2326779
Isabella Brohman, Gabriel Blank, Hana Mitchell, Eve Dubé, Julie A Bettinger
{"title":"Opportunities for HPV vaccine education in school-based immunization programs in British Columbia, Canada: A qualitative study.","authors":"Isabella Brohman, Gabriel Blank, Hana Mitchell, Eve Dubé, Julie A Bettinger","doi":"10.1080/21645515.2024.2326779","DOIUrl":"10.1080/21645515.2024.2326779","url":null,"abstract":"<p><p>Despite the availability of school-based immunization programs (SBIPs) in Canada, human papillomavirus (HPV) vaccine uptake remains suboptimal. Vaccine education may improve vaccine uptake among adolescents. The objective of this qualitative study was to identify opportunities for HPV vaccine education in British Columbia, Canada, by exploring the perspectives of students, parents, school staff, and public health nurses on the current SBIP. Individual semi-structured interviews were conducted with adult participants and focus groups were conducted with grade 6 students between November 2019 and May 2020. The interviews and focus groups were transcribed and then analyzed using reflexive thematic analysis. Opportunities for HPV vaccine education were identified in three themes: 1) making SBIPs student-centered; 2) adopting a collaborative and interdisciplinary approach to vaccine education; and 3) actualizing parent education opportunities. Broad support existed for a formal, collaborative HPV grade 6 vaccine curriculum delivered by teachers and public health nurses to provide evidence-based health information. Participants voiced that the curriculum should integrate students' perspectives on topics of interest and address needle associated pain and anxiety. Parents were identified as the primary vaccine decisionmakers, therefore, participants stated it was crucial to also provide parent-directed vaccine education as part of SBIP. Our findings support the development of a collaborative HPV vaccine curriculum directed to and informed by students and parents to buttress current SBIPs in British Columbia.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
COVID-19 vaccination perspectives among patients with Long COVID: A qualitative study. 长COVID患者对COVID-19疫苗接种的看法:一项定性研究。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-03-26 DOI: 10.1080/21645515.2024.2327663
Sarah R MacEwan, Saurabh Rahurkar, Willi L Tarver, Alice A Gaughan, Laura J Rush, Andrew Schamess, Ann Scheck McAlearney
{"title":"COVID-19 vaccination perspectives among patients with Long COVID: A qualitative study.","authors":"Sarah R MacEwan, Saurabh Rahurkar, Willi L Tarver, Alice A Gaughan, Laura J Rush, Andrew Schamess, Ann Scheck McAlearney","doi":"10.1080/21645515.2024.2327663","DOIUrl":"10.1080/21645515.2024.2327663","url":null,"abstract":"<p><p>Individuals who have Long COVID may have unique perspectives about COVID-19 vaccination due to the significant impact that COVID-19 has had on their lives. However, little is known about the specific vaccination perspectives among this patient population. The goal of our study was to improve our understanding of perspectives about COVID-19 vaccines among individuals with Long COVID. Interviews were conducted with patients receiving care at a post-COVID recovery clinic. Deductive thematic analysis was used to characterize participant perspectives according to the vaccine acceptance continuum framework, which recognizes a spectrum from vaccine acceptance to refusal. From interviews with 21 patients, we identified perspectives across the continuum of vaccine acceptance. These perspectives included acceptance of vaccines to prevent future illness, concerns about vaccine side effects on Long COVID symptoms, and refusal of vaccines due to perceived natural immunity. A limitation of our study is that these perspectives are specific to individuals receiving care at one post-COVID recovery clinic. In conclusion, our study demonstrates that some patients with Long COVID are uncertain about COVID-19 vaccines and boosters but may also be amenable to conversations that impact future vaccination acceptance. Patient perspectives should be considered when communicating recommendations for COVID-19 vaccinations to this population.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10978020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced immunity against SARS-CoV-2 in returning Chinese individuals. 提高归国华侨对 SARS-CoV-2 的免疫力。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-01-08 DOI: 10.1080/21645515.2023.2300208
Runyu Yuan, Huimin Chen, Lina Yi, Xinxin Li, Ximing Hu, Xing Li, Huan Zhang, Pingping Zhou, Chumin Liang, Huifang Lin, Lilian Zeng, Xue Zhuang, QianQian Ruan, Yueling Chen, Yingyin Deng, Zhe Liu, Jing Lu, Jianpeng Xiao, Liang Chen, Xincai Xiao, Jing Li, Baisheng Li, Yan Li, Jianfeng He, Jiufeng Sun
{"title":"Enhanced immunity against SARS-CoV-2 in returning Chinese individuals.","authors":"Runyu Yuan, Huimin Chen, Lina Yi, Xinxin Li, Ximing Hu, Xing Li, Huan Zhang, Pingping Zhou, Chumin Liang, Huifang Lin, Lilian Zeng, Xue Zhuang, QianQian Ruan, Yueling Chen, Yingyin Deng, Zhe Liu, Jing Lu, Jianpeng Xiao, Liang Chen, Xincai Xiao, Jing Li, Baisheng Li, Yan Li, Jianfeng He, Jiufeng Sun","doi":"10.1080/21645515.2023.2300208","DOIUrl":"10.1080/21645515.2023.2300208","url":null,"abstract":"<p><p>Global COVID-19 vaccination programs effectively contained the fast spread of SARS-CoV-2. Characterizing the immunity status of returned populations will favor understanding the achievement of herd immunity and long-term management of COVID-19 in China. Individuals were recruited from 7 quarantine stations in Guangzhou, China. Blood and throat swab specimens were collected from participants, and their immunity status was determined through competitive ELISA, microneutralization assay and enzyme-linked FluoroSpot assay. A total of 272 subjects were involved in the questionnaire survey, of whom 235 (86.4%) were returning Chinese individuals and 37 (13.6%) were foreigners. Blood and throat swab specimens were collected from 108 returning Chinese individuals. Neutralizing antibodies against SARS-CoV-2 were detected in ~90% of returning Chinese individuals, either in the primary or the homologous and heterologous booster vaccination group. The serum NAb titers were significantly decreased against SARS-CoV-2 Omicron BA.5, BF.7, BQ.1 and XBB.1 compared with the prototype virus. However, memory T-cell responses, including specific IFN-γ and IL-2 responses, were not different in either group. Smoking, alcohol consumption, SARS-CoV-2 infection, COVID-19 vaccination, and the time interval between last vaccination and sampling were independent influencing factors for NAb titers against prototype SARS-CoV-2 and variants of concern. The vaccine dose was the unique common influencing factor for Omicron subvariants. Enhanced immunity against SARS-CoV-2 was established in returning Chinese individuals who were exposed to reinfection and vaccination. Domestic residents will benefit from booster homologous or heterologous COVID-19 vaccination after reopening of China, which is also useful against breakthrough infection.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10793704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative immunogenicity and neutralizing antibody responses post heterologous vaccination with CoronaVac (Sinovac) and Vaxzevria (AstraZeneca) in HIV-infected patients with varying CD4+ T lymphocyte counts. 在不同 CD4+ T 淋巴细胞数量的 HIV 感染者中接种异源疫苗 CoronaVac(希诺威)和 Vaxzevria(阿斯利康)后的免疫原性和中和抗体反应比较。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-01-31 DOI: 10.1080/21645515.2024.2309734
Sorawit Chittrakarn, Pisud Siripaitoon, Sarunyou Chusri, Siripen Kanchanasuwan, Boonsri Charoenmak, Thanaporn Hortiwakul, Phaiwon Kantikit, Narongdet Kositpantawong
{"title":"Comparative immunogenicity and neutralizing antibody responses post heterologous vaccination with CoronaVac (Sinovac) and Vaxzevria (AstraZeneca) in HIV-infected patients with varying CD4+ T lymphocyte counts.","authors":"Sorawit Chittrakarn, Pisud Siripaitoon, Sarunyou Chusri, Siripen Kanchanasuwan, Boonsri Charoenmak, Thanaporn Hortiwakul, Phaiwon Kantikit, Narongdet Kositpantawong","doi":"10.1080/21645515.2024.2309734","DOIUrl":"10.1080/21645515.2024.2309734","url":null,"abstract":"<p><p>The immune response to heterologous coronavirus disease (COVID-19) vaccination in people living with HIV (PLWH) is still unclear. Herein, our prospective cohort study aimed to compare the immune response of heterologous vaccination with CoronaVac (Sinovac) and Vaxzevria (AstraZeneca) between PLWH having CD4 counts ≤ 200 cells/µL (low CD4+) and > 200 cells/µL (high CD4+). Anti-receptor-binding domain (RBD) immunoglobulin G (IgG) levels and the percentage inhibition of neutralizing antibodies (nAbs) were analyzed at 2 and 12 weeks after immunization. Participants in the low and high CD4+ groups had mean CD4+ counts of 139 and 575 cell/µL, respectively. Two and 12 weeks after immunization, in the low CD4 group, the median anti-RBD-IgG levels were 159 IU/mL and 143 IU/mL, respectively, whereas the nAb level was 71% and decreased to 47.2%, respectively. Contrarily, the median anti-RBD-IgG levels in the high CD4+ group were 273 IU/mL and 294 IU/mL, respectively, whereas the nAb levels were 89.3% and relatively stable at 81.6%. However, although immune responses between the two study groups were not significantly different, a decline in nAb levels was observed at 12 weeks in the low CD4+ group. Therefore, a COVID-19 booster vaccine dose is suggested for immunoprotection.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10841008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139651994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ex vivo observation of Pythium insidiosum-antigen treated neutrophils on three Pythium insidiosum strains isolated from vascular pythiosis patients. 体内观察经脓疱疮抗原处理的中性粒细胞对从血管脓疱病患者体内分离出的三种脓疱疮菌株的影响。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-02-05 DOI: 10.1080/21645515.2024.2304372
Sadeep Medhasi, Apichaya Sriwarom, Nitipong Permpalung, Pattama Torvorapanit, Rongpong Plongla, Ariya Chindamporn, Navaporn Worasilchai
{"title":"<i>Ex vivo</i> observation of <i>Pythium insidiosum</i>-antigen treated neutrophils on three <i>Pythium insidiosum</i> strains isolated from vascular pythiosis patients.","authors":"Sadeep Medhasi, Apichaya Sriwarom, Nitipong Permpalung, Pattama Torvorapanit, Rongpong Plongla, Ariya Chindamporn, Navaporn Worasilchai","doi":"10.1080/21645515.2024.2304372","DOIUrl":"10.1080/21645515.2024.2304372","url":null,"abstract":"<p><p>The mechanisms of <i>Pythium insidiosum</i>-antigen (PIA) immunotherapy activating a patient's immune system are unknown. We evaluated the interleukin-8 (IL-8) serum levels during <i>P. insidiosum</i> infection and after vaccination with PIA in vascular pythiosis cases. Furthermore, we studied the anti-<i>P. insidiosum</i> activity of neutrophils stimulated with various concentrations of PIA <i>ex vivo</i> in 3 strains of <i>P. insidiosum</i> isolated from vascular pythiosis patients. IL-8 serum levels were evaluated using the ELISA technique. We assessed the effect of PIA-stimulated neutrophils on the viability of zoospores using MTT assay, visualized neutrophil extracellular trap (NET) formation via microscopy, and measured the levels of double-stranded DNA (dsDNA) using PicoGreen dsDNA quantitation assay in 3 strains of <i>P. insidiosum</i> isolated from vascular pythiosis patients. Serum levels of IL-8 gradually lowered from the early to the end phases of vaccination with PIA among the surviving group of vascular pythiosis cases. Neutrophils stimulated with 0.01 µg/ml PIA reduced zoospore viability significantly compared to PIA-unstimulated neutrophils for strain 1 and strain 3 (<i>p</i> < .05). Neutrophils stimulated with 0.01, 0.1, 1, and 10 µg/ml PIA exhibited significantly lower zoospore viability than PIA-unstimulated neutrophils for strain 2 (<i>p</i> < .05). IL-8 can be used as a biomarker for monitoring vascular pythiosis cases treated with the PIA vaccine. Also, anti-<i>P. insidiosum</i> activity of PIA-stimulated neutrophils was probably due to the disruption of cellular activity in zoospores rather than the mechanisms based on the formation of NETs.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139693331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cost-effectiveness analysis of vaccination strategies against meningococcal disease for children under nine years of age in China. 中国九岁以下儿童脑膜炎球菌病疫苗接种策略的成本效益分析。
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-02-13 DOI: 10.1080/21645515.2024.2313872
Haonan Zhang, Haijun Zhang, Hai Fang
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引用次数: 0
Reconsidering the inclusion of Ladapo's work in the meta-analysis: Validity concerns and implications. 重新考虑将拉达波的工作纳入荟萃分析:有效性问题及影响
IF 4.8 4区 医学
Human Vaccines & Immunotherapeutics Pub Date : 2024-12-31 Epub Date: 2024-02-15 DOI: 10.1080/21645515.2024.2315711
Borja Somovilla Del Saz
{"title":"Reconsidering the inclusion of Ladapo's work in the meta-analysis: Validity concerns and implications.","authors":"Borja Somovilla Del Saz","doi":"10.1080/21645515.2024.2315711","DOIUrl":"10.1080/21645515.2024.2315711","url":null,"abstract":"<p><p>This is a response to Marchand & Masoud's response letter regarding my criticism \"Response to Dr. Somovilla del Saz's letter to the editor regarding \"Risk of all-cause and cardiac-related mortality after vaccination against COVID-19: A meta-analysis of self-controlled case series studies.\"\" The response is a defense of the initial criticism to the paper regarding the validity of the inclusion of Ladapo´s paper.</p>","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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