Revista Brasileira De Parasitologia Veterinaria最新文献_第4页

Efficacy of selenium- and tellurium-based organocompounds against ruminant gastrointestinal nematodes in vitro. 硒和碲基有机化合物体外抗反刍动物胃肠道线虫的效果。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-06-15 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026017

Taynara Gabriele Ribeiro Piano, Irineu Romero Neto, Leandro Piovan, Leandro Bittencourt de Oliveira, Alda Lúcia Gomes Monteiro, Tay Zugman, Bernardo Ariel Schorr Zotz, Ursula Yaeko Yoshitani, Marcelo Beltrão Molento

{"title":"Efficacy of selenium- and tellurium-based organocompounds against ruminant gastrointestinal nematodes in vitro.","authors":"Taynara Gabriele Ribeiro Piano, Irineu Romero Neto, Leandro Piovan, Leandro Bittencourt de Oliveira, Alda Lúcia Gomes Monteiro, Tay Zugman, Bernardo Ariel Schorr Zotz, Ursula Yaeko Yoshitani, Marcelo Beltrão Molento","doi":"10.1590/S1984-29612026017","DOIUrl":"10.1590/S1984-29612026017","url":null,"abstract":"Gastrointestinal nematode (GIN) infections significantly impact the health of ruminants. The indiscriminate use of anthelmintics contributes to the selection of drug-resistant parasites. Compounds containing selenium (Se) and tellurium (Te) have emerged as promising alternatives. This study aimed to evaluate the anthelmintic activity of diphenyl diselenide (PhSe)2, phenylselenenyl chloride (4-Cl-PhSe)2, and ammonium trichloro (dioxoethylene-O,O') tellurate (AS-101) on eggs and larvae (L3) of GIN. The compounds were tested using egg-hatch and larval-migration inhibition tests. Additionally, the possible in vitro cytotoxic effects were evaluated using the AlamarBlue assay. (PhSe)2 exhibited the highest ovicidal effect, with a 50% inhibitory concentration (IC50) of 1.801 mmol L-1 for GIN isolated from sheep and 1.845 mmol L-1 for GIN from cattle. AS-101 showed the highest larvicidal activity, with IC50 values of 0.976 mmol L-1 for GIN of sheep and 1.035 mmol L-1 for GIN of cattle. Combinations of (PhSe)2 with ivermectin (IVM) showed a mild synergistic effect, with an additive interaction of 13.33%. Among the tested compounds, (PhSe)2 showed the lowest cytotoxicity in LLC-MK2 cells. Taken together, these data highlight the therapeutic potential of the novel molecules.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 2","pages":"e012825"},"PeriodicalIF":1.2,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localized demodicosis associated with Demodex canis and Demodex cornei infestation in a dog from Mexico. 墨西哥犬的局部蠕形螨病与犬蠕形螨和角蠕形螨感染有关。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-06-15 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026018

Elsa Carolina Landeros Gálvez, Lucía Teresa Fuentes Guardiola, Jerónimo Landeros Flores, Oscar Ángel Sánchez Flores

{"title":"Localized demodicosis associated with Demodex canis and Demodex cornei infestation in a dog from Mexico.","authors":"Elsa Carolina Landeros Gálvez, Lucía Teresa Fuentes Guardiola, Jerónimo Landeros Flores, Oscar Ángel Sánchez Flores","doi":"10.1590/S1984-29612026018","DOIUrl":"10.1590/S1984-29612026018","url":null,"abstract":"Canine demodicosis is a cutaneous parasitic disease caused by excessive proliferation of mites of the genus Demodex. This study reports a case of localized demodicosis associated with a concurrent infestation by Demodex canis and Demodex cornei in an adult mixed-breed female dog from the state of Coahuila, northern Mexico. The animal presented two focal alopecic lesions in the nasal and perioral regions, with no evidence of secondary infection. Deep skin scrapings were collected and examined by light microscopy. Species-level identification was based on diagnostic morphological characters, including opisthosomal shape and proportions, supracoxal spine morphology, and pharyngeal bulb structure, confirming the simultaneous presence of both species. This study represents the first confirmed record of D. canis and D. cornei in domestic dogs from the state of Coahuila and contributes to the knowledge of geographic distribution and diversity of demodecid mites in Mexico.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 3","pages":"e006726"},"PeriodicalIF":1.2,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ascaridia pavo n. sp. (Nematoda: Ascaridida), a new species of nematode in peafowl. 孔雀体内线虫一新种——帕氏蛔虫（线虫纲：蛔虫）。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026006

Raul Henrique da Silva Pinheiro, Roberto Rafael de Queiroz Pereira Rodrigues, Raimundo Nonato Moraes Benigno, Luis Augusto Araújo Dos Santos Ruffeil, Elane Guerreiro Giese

{"title":"Ascaridia pavo n. sp. (Nematoda: Ascaridida), a new species of nematode in peafowl.","authors":"Raul Henrique da Silva Pinheiro, Roberto Rafael de Queiroz Pereira Rodrigues, Raimundo Nonato Moraes Benigno, Luis Augusto Araújo Dos Santos Ruffeil, Elane Guerreiro Giese","doi":"10.1590/S1984-29612026006","DOIUrl":"10.1590/S1984-29612026006","url":null,"abstract":"A new Ascaridiidae has been found parasitizing Pavo cristatus, an exotic bird bred on a commercial farm in the Pará Amazon. The new species of Ascaridia Dujardin, 1845 is described on the basis of light and scanning microscopy findings. Morphologically, the new species shares characteristics compatible with the other species of Ascaridia that parasitize birds in Brazil, however, the new species differs from A. pintoi and A. ornata in the length and absence of a wing on the spicules, A. hermaphrodita, A. serrata, A. columbae, A. galli, A. lineata, A. magalhaesi, A. numidae, A. orthocerca, A. pterophora and A. sergiomeirai in the distribution and number of caudal papillae in males, A. amblymoria in the presence of a single papilla on the upper lip, associated with these characteristics most species differ in host order, with only A. columbae, A. galli and A. lineata, parasites of birds in the Phasianidae family. The new taxon adds morphological data to the parasitic biodiversity of commercially reared birds in Brazil.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 1","pages":"e011525"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of the emerging tick-borne pathogen, Tamdy virus, in Dermacentor marginatus ticks, southeastern Türkiye. <s:1>基耶省东南部边缘革蜱中新发蜱传病原体Tamdy病毒的检测。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026009

Ender Dincer, Mehmet Ozkan Timurkan

{"title":"Detection of the emerging tick-borne pathogen, Tamdy virus, in Dermacentor marginatus ticks, southeastern Türkiye.","authors":"Ender Dincer, Mehmet Ozkan Timurkan","doi":"10.1590/S1984-29612026009","DOIUrl":"10.1590/S1984-29612026009","url":null,"abstract":"Tamdy virus (TAMV) is an emerging tick-borne virus in the family Nairoviridae, identified as a human pathogen causing febrile illness after tick exposure. This study aimed to detect and characterise TAMV in ticks collected from non-endemic regions of Türkiye. A total of 529 ticks were sampled from six provinces and identified as Haemaphysalis parva (27%), Rhipicephalus bursa (26%), Haemaphysalis punctata (20.7%), Dermacentor marginatus (17.2%), and Ixodes ricinus (8.8%). Screening with a single-step generic PCR revealed one TAMV-positive pool out of 96. For characterisation, the S segment of TAMV was amplified using two primer sets. Phylogenetic analyses of the partial L gene and full S segment showed that the sequence from Osmaniye sample clustered closely with the TT1 isolate from Konya, Türkiye. Amino acid comparison indicated that Turkish isolates were more similar to each other than to those from Russia and China, forming a distinct clade within TAMV. Minor amino acid differences between the Turkish isolates suggest that TAMV diversity in Türkiye is shaped by internal evolution rather than external introductions. These findings highlight the importance of continuous surveillance of tick-borne zoonotic viruses and suggest that TAMV may have a wider vector range and geographic distribution than previously recognised.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 1","pages":"e016325"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal dynamics in the parasite communities of corocoro grunt Orthopristis rubra (Actinopterygii: Haemulidae) from Sepetiba Bay, southeastern Brazil: a comparative study from 1991 to 2024. 1991 - 2024年巴西东南部Sepetiba湾斑冠corcoro grunt Orthopristis rubra（放线翼虫科：Haemulidae）寄生虫群落的时间动态比较研究

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026012

Adriana Rodrigues Mainenti, Fabiano Paschoal, Thiago Dos Santos Cardoso, Raquel de Oliveira Simões, José Luis Luque

{"title":"Temporal dynamics in the parasite communities of corocoro grunt Orthopristis rubra (Actinopterygii: Haemulidae) from Sepetiba Bay, southeastern Brazil: a comparative study from 1991 to 2024.","authors":"Adriana Rodrigues Mainenti, Fabiano Paschoal, Thiago Dos Santos Cardoso, Raquel de Oliveira Simões, José Luis Luque","doi":"10.1590/S1984-29612026012","DOIUrl":"10.1590/S1984-29612026012","url":null,"abstract":"This study investigated the temporal dynamics of metazoan parasite communities of the corocoro grunt Orthopristis rubra (Haemulidae) from State of Rio de Janeiro, Sepetiba Bay, southeastern Brazil, comparing samples collected in 1991-1992, 2011-2012, and 2022-2024. A total of 180 fish were examined, revealing 26 parasite taxa belonging to seven higher groups. The helminth community showed aggregated distributions and a clear temporal pattern in abundance and diversity. Multivariate analyses (nMDS and PERMANOVA) indicated significant but moderate differences among periods, with increased species richness, evenness, and diversity in recent years. Hill diversity profiles revealed higher Shannon and Simpson indices in 2022-2024, suggesting greater balance and reduced dominance. These trends indicate gradual reorganization of parasite assemblages, likely associated with ecological recovery of Sepetiba Bay. The persistence of core digeneans and the appearance of new taxa reflect shifts in food-web structure and host-parasite interactions. The results reinforce the value of long-term parasitological data as bioindicators of ecosystem change in coastal environments under anthropogenic pressure.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 2","pages":"e016925"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of water temperature, sedimentation and fecal refrigeration times on the recovery of Dictyocaulus viviparus larvae by two diagnostic techniques. 水温、沉淀物和粪便冷藏次数对两种诊断技术下胎胎虫幼虫恢复的影响。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026015

Bruno de Oliveira Telles Ferreira, Lais Sperandio Cassani, Ygor Henrique da Silva, Barbara Rauta de Avelar, Manoella Dantas Medeiros, Alyne Mendonça Lima, Laritssa Andrade Pinheiro Magalhães, Thaís Ribeiro Correia

{"title":"Influence of water temperature, sedimentation and fecal refrigeration times on the recovery of Dictyocaulus viviparus larvae by two diagnostic techniques.","authors":"Bruno de Oliveira Telles Ferreira, Lais Sperandio Cassani, Ygor Henrique da Silva, Barbara Rauta de Avelar, Manoella Dantas Medeiros, Alyne Mendonça Lima, Laritssa Andrade Pinheiro Magalhães, Thaís Ribeiro Correia","doi":"10.1590/S1984-29612026015","DOIUrl":"10.1590/S1984-29612026015","url":null,"abstract":"Bovine dictyocaulosis, caused by Dictyocaulus viviparus, is a respiratory infection that leads to economic losses in livestock farming. The objective of this study was to compare two techniques for the diagnosis of D. viviparus. Residual fecal samples from routine diagnostics known to be positive were homogenized and subjected to the techniques proposed by modified Rugai, and Ueno techniques. We verified the effects of fecal refrigeration time (+24h and +48h), sedimentation time (2h and 12h), and water temperature (22°C and 46°C) on the recovery and sensitivity of first-stage larva (L1) diagnosis. Fecal refrigeration time (24h vs. 48h) did not significantly affect the number of recovered larvae, demonstrating that samples can be safely stored for up to two days. Room temperature water demonstrated a sensitivity below 75% in the recovery of D. viviparus during the first two hours in both techniques, increasing to 100% with 12 hours of sedimentation. However, heating the water to 46ºC proved to be the key factor: larval recovery became equal between the techniques, achieving 100% sensitivity in both after only two hours. We conclude that both techniques have similar sensitivity, making both valid for the recovery and diagnosis of D. viviparus.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 2","pages":"e002526"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ocimum micranthum essential oil as a sustainable phytotherapeutic alternative against Haemonchus contortus. 微红花精油作为抗弯血螨的可持续植物治疗替代品。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-296120260011

Andreza Pereira Braga, Matheus Luiggi Freitas Barbosa, Raphael Ferreira Oliveira, Karin Vitória Maia Mendonça Ferreira, Sara Stefane da Silva Cardoso, Rayane de Araújo Souza, Rita de Cássia Alves Pereira, Francisco Flávio da Silva Lopes, Gracielle Araújo Frota, Selene Maia de Morais, Letícia Oliveira da Rocha, Wesley Lyeverton Correia Ribeiro, Lorena Mayana Beserra de Oliveira

{"title":"Ocimum micranthum essential oil as a sustainable phytotherapeutic alternative against Haemonchus contortus.","authors":"Andreza Pereira Braga, Matheus Luiggi Freitas Barbosa, Raphael Ferreira Oliveira, Karin Vitória Maia Mendonça Ferreira, Sara Stefane da Silva Cardoso, Rayane de Araújo Souza, Rita de Cássia Alves Pereira, Francisco Flávio da Silva Lopes, Gracielle Araújo Frota, Selene Maia de Morais, Letícia Oliveira da Rocha, Wesley Lyeverton Correia Ribeiro, Lorena Mayana Beserra de Oliveira","doi":"10.1590/S1984-296120260011","DOIUrl":"10.1590/S1984-296120260011","url":null,"abstract":"This study is the first to evaluate the anthelmintic activity of Ocimum micranthum essential oil against Haemonchus contortus. The oil was chemically characterized using gas chromatography-mass spectrometry. Anthelmintic activity was assessed using the egg hatching test (EHT), larval development test (LDT), larval migration inhibition test (LMIT), and adult worm motility test (AWMT) against a multidrug-resistant H. contortus isolate. Following AWMT, oil-induced morphological and ultrastructural alterations were investigated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Eugenol was the major oil component (78.44%). The oil exhibited anthelmintic activity in all assays, with EC50 values of 0.19 (EHT), 0.031 (LDT), 0.63 (LMIT) and 0.046 (AWMT) mg/mL. At 0.25 mg/mL, complete inhibition of adult motility was observed at the first evaluation time point (3 h post-exposure). SEM revealed damage to the cuticle, buccal capsule, and vulvar flap, whereas TEM demonstrated alterations in internal tissues. Ocimum micranthum essential oil was effective against H. contortus eggs, larvae, and adults. Therefore, further studies evaluating its toxicological safety and efficacy in small ruminants are required to validate its potential as an anthelmintic.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 2","pages":"e001526"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy, toxicity and histopathological analysis of Astrocaryum aculeatum oil solid lipid nanoparticles on Colossoma macropomum infected by parasitic dactylogyrideans. 刺心豆油固体脂纳米颗粒对巨巨像寄生指线虫感染的疗效、毒性及组织病理学分析。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026016

Bruna David Brito, Marcela Nunes Videira, Abthyllane Amaral de Carvalho, Maiara Ferreira Martins, Victor Hugo Souza Marinho, Irlon Maciel Ferreira, Eliane Tie Oba Yoshioka, Marcos Tavares-Dias

{"title":"Efficacy, toxicity and histopathological analysis of Astrocaryum aculeatum oil solid lipid nanoparticles on Colossoma macropomum infected by parasitic dactylogyrideans.","authors":"Bruna David Brito, Marcela Nunes Videira, Abthyllane Amaral de Carvalho, Maiara Ferreira Martins, Victor Hugo Souza Marinho, Irlon Maciel Ferreira, Eliane Tie Oba Yoshioka, Marcos Tavares-Dias","doi":"10.1590/S1984-29612026016","DOIUrl":"10.1590/S1984-29612026016","url":null,"abstract":"This study investigated the efficacy of Astrocaryum aculeatum fixed oil solid lipid nanoparticles (SLNs) on dactylogyridean parasites of Colossoma macropomum gills and effects on hematology and gill histology. The main fatty acid component of A. aculeatum oil was oleic acid (68.0%). Among the concentrations of A. aculeatum fixed oil SLNs tested (250-1000 mg/L), the fish tolerated only 250 mg/L, which was the concentration used in the consecutive five baths of 1 h per day. Two control groups were also used: one with myristic acid + Tween 80 and another with only water from the cultivation tank. The baths have no efficacy against Anacanthorus spathulatus, Notozothecium janauachensis, and Mymarothecium boegeri from C. macropomum gills. Detachment of the gill lamellar epithelium, lamellar epithelial hyperplasia, lamellar hyperplasia with fusion, and aneurysm were damages caused by parasites and not by the treatments. Total number of lymphocytes decreased in the fish exposed to baths with 250 mg/L of A. aculeatum fixed oil SLNs, while other blood parameters were not affected. Although the treatments with A. aculeatum fixed oil SLNs were not effective against parasitic dactylogyrideans as expected, they were not toxic for C. macropomum, because they did not provoke alterations in gills and in physiology.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 2","pages":"e015725"},"PeriodicalIF":1.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Metarhizium spp. against gastrointestinal nematodes in goats and evidence of conidia infection through the integument. 绿僵菌对山羊胃肠道线虫的防治效果及通过被膜感染分生孢子的证据。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-296120260010

Ially de Almeida Moura, Antônio Wesley Oliveira da Silva, Mayara Macêdo Barrozo, Thaís Almeida Corrêa, Patrícia Silva Gôlo, Isabele da Costa Ângelo, Caio Márcio de Oliveira Monteiro, Éverton Kort Kamp Fernandes, Vânia Rita Elias Pinheiro Bittencourt, Alexandre Dias Munhoz, Wendell Marcelo de Souza Perinotto

{"title":"Efficacy of Metarhizium spp. against gastrointestinal nematodes in goats and evidence of conidia infection through the integument.","authors":"Ially de Almeida Moura, Antônio Wesley Oliveira da Silva, Mayara Macêdo Barrozo, Thaís Almeida Corrêa, Patrícia Silva Gôlo, Isabele da Costa Ângelo, Caio Márcio de Oliveira Monteiro, Éverton Kort Kamp Fernandes, Vânia Rita Elias Pinheiro Bittencourt, Alexandre Dias Munhoz, Wendell Marcelo de Souza Perinotto","doi":"10.1590/S1984-296120260010","DOIUrl":"10.1590/S1984-296120260010","url":null,"abstract":"The search for sustainable alternatives to manage gastrointestinal nematodes in small ruminants has intensified due to rising anthelmintic resistance. This study evaluated in vitro the efficacy of several Brazilian isolates of the genus Metarhizium spp. on gastrointestinal nematodes (GINs) of goats which were naturally infected with mixed populations predominantly composed of Haemonchus spp. (63%), Trichostrongylus spp. (35%), and Oesophagostomum spp. (2%). Biological assays were conducted using quantitative coproculture after 10 days of incubation, and SEM was performed after 72 h of fungus-larva interaction. Isolates LCM S05, LCM S10, LCM S11, LCM S13, LCM S14, and LCM S15 promoted larval reduction of: 50.24%, 43.29%, 44.99%, 55.45%, 52.52%, and 67.95%, respectively. Isolate LCM S15, which showed the greatest reduction in larvae, was tested at concentrations of 1.0 × 106, 1.0 × 107, and 1.0 × 108 conidia/mL, resulting in reductions in larvae of 36.90%, 50.17%, and 68.28%, respectively. In SEM, photomicrographs demonstrated that conidia adhered to and germinated on the nematode integument. This study demonstrates the potential of Brazilian isolates of Metarhizium spp. for the control of GINs in goats and highlights the route of fungal infection through the integument.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 1","pages":"e017125"},"PeriodicalIF":1.2,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147976763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and molecular characterization of Trypanosoma theileri in cattle from the municipality of Vassouras, Rio de Janeiro, Brazil. 巴西里约热内卢瓦苏拉斯市牛中牛氏锥虫的检测和分子特征。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/S1984-29612026008

Jônathan David Ribas Chagas, Matheus Dias Cordeiro, Aline Maria Andrade da Silva, Ana Paula Martinez de Abreu, Renata Fernandes Ferreira de Moraes, Erica Cristina Rocha Roier, Maristela Peckle, Bruna de Azevedo Baêta

{"title":"Detection and molecular characterization of Trypanosoma theileri in cattle from the municipality of Vassouras, Rio de Janeiro, Brazil.","authors":"Jônathan David Ribas Chagas, Matheus Dias Cordeiro, Aline Maria Andrade da Silva, Ana Paula Martinez de Abreu, Renata Fernandes Ferreira de Moraes, Erica Cristina Rocha Roier, Maristela Peckle, Bruna de Azevedo Baêta","doi":"10.1590/S1984-29612026008","DOIUrl":"10.1590/S1984-29612026008","url":null,"abstract":"This study aimed to detect, using parasitological and molecular methods, and to genetically characterize Trypanosoma spp. in cattle from the municipality of Vassouras, Rio de Janeiro, Brazil. A total of 226 blood samples were collected from dairy cattle across eight farms, and DNA was extracted for PCR amplification of the 18S rRNA gene of Trypanosoma spp. In parasitological examinations, including blood smears and the Woo's technique, no trypomastigote forms of Trypanosoma spp. were observed. Molecular detection via 18S rRNA identified the parasite in 0.88% (2/226) of the samples. The resulting amplicons were sequenced, aligned, and compared with GenBank sequences for phylogenetic analysis. The analyses confirmed 100% identity with T. theileri, prompting further molecular characterization using the 24Sα rRNA (257 pb) and CatL (295 pb) genes. Phylogenetic analysis of T. theileri indicated circulation of the TthI lineage, encompassing genotype IB, in the municipality of Vassouras, Rio de Janeiro. These findings expand knowledge on the geographic distribution of T. theileri in Brazil and underscore the importance of monitoring cattle herds, considering the economic and health significance of trypanosomatids.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"35 1","pages":"e015525"},"PeriodicalIF":1.2,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147976713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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