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Widespread recombinase expression using FLPeR (flipper) mice. 重组酶在FLPeR (flipper)小鼠中的广泛表达。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
F W Farley, P Soriano, L S Steffen, S M Dymecki
{"title":"Widespread recombinase expression using FLPeR (flipper) mice.","authors":"F W Farley,&nbsp;P Soriano,&nbsp;L S Steffen,&nbsp;S M Dymecki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As conditional genetic strategies advance, the need for multiple site-specific recombinase systems has emerged. To meet this need in part, we have targeted the constitutive ROSA26 locus to create a mouse strain with generalized expression of the enhanced version of the site-specific recombinase FLP (FLPe). This strain is designated FLPeR (\"flipper\"). Using this strain, extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. FLPeR mice therefore serve two important functions: as a source of many different FLPe-expressing primary cell lines and as a deleter strain. Moreover, because the FLPeR mouse is a 129-derived strain, a 129 genetic background can be preserved when crossed to most ES cell-derived mice. This enables conditional genetic alterations to be maintained on a standard background, a feature important for obtaining reproducible results and genetically defined controls.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"106-10"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA chips and development: prospects and problems. DNA芯片及其发展:前景与问题。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
D G Schwarz
{"title":"DNA chips and development: prospects and problems.","authors":"D G Schwarz","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"134"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Related function of mouse SOX3, SOX9, and SRY HMG domains assayed by male sex determination. 小鼠SOX3、SOX9和SRY HMG结构域的相关功能
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
D E Bergstrom, M Young, K H Albrecht, E M Eicher
{"title":"Related function of mouse SOX3, SOX9, and SRY HMG domains assayed by male sex determination.","authors":"D E Bergstrom, M Young, K H Albrecht, E M Eicher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sox genes encode proteins related to each other, and to the sex determining gene Sry, by the presence of a DNA binding motif known as the HMG domain. Although HMG domains can bind to related DNA sequences, Sox gene products may achieve target gene specificity by binding to preferred target sequences or by interacting with specific partner proteins. To assess their functional similarities, we replaced the HMG box of Sry with the HMG box of Sox3 or Sox9 and tested whether these constructs caused sex reversal in XX mice. Our results indicate that such chimeric transgenes can functionally replace Sry and elicit development of testis cords, male patterns of gene expression, and elaboration of male secondary sexual characteristics. This implies that chimeric SRY proteins with SOX HMG domains can bind to and regulate SRY target genes and that potential SRY partner factor interactions are not disrupted by HMG domain substitutions. genesis 28:111-124, 2000.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"111-24"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700755/pdf/nihms-72362.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developmental effects of a chimeric ultraspiracle gene derived from Drosophila and Chironomus. 果蝇和手蝇嵌合超囊基因的发育效应。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01 DOI: 10.1002/1526-968x(200011/12)28:3/4<125::aid-gene50>3.3.co;2-j
V C Henrich, M E Vogtli, C Antoniewski, M Spindler-Barth, S Przibilla, M Noureddine, M Lezzi
{"title":"Developmental effects of a chimeric ultraspiracle gene derived from Drosophila and Chironomus.","authors":"V C Henrich,&nbsp;M E Vogtli,&nbsp;C Antoniewski,&nbsp;M Spindler-Barth,&nbsp;S Przibilla,&nbsp;M Noureddine,&nbsp;M Lezzi","doi":"10.1002/1526-968x(200011/12)28:3/4<125::aid-gene50>3.3.co;2-j","DOIUrl":"https://doi.org/10.1002/1526-968x(200011/12)28:3/4<125::aid-gene50>3.3.co;2-j","url":null,"abstract":"<p><p>The ultraspiracle (usp) gene encodes a nuclear receptor that forms a heterodimer with the ecdysone receptor (EcR) to mediate transcriptional responses to the insect steroid hormone, 20-hydroxyecdysone (20HE). The responses ultimately elicit changes associated with molting and metamorphosis. Although Ultraspiracle (USP) is required at several developmental times, it is unclear whether USP plays stage-specific roles in Drosophila. A chimeric transgene (d/cusp), produced by replacing the ligand-binding domain (LBD) of Drosophila USP with the equivalent domain from another Diptera, Chironomus tentans, was tested for its ability to rescue Drosophila usp mutants from early larval lethality. A single copy of the d/cusp was sufficient to rescue transformants from several lines through larval development but they died suddenly during the late third instar. Additional doses of d/cusp were required to allow survival through the adult stage, but they did not restore a normal prepupal contraction. Thus, the arrest at the onset of metamorphosis apparently is caused by the impaired ability of the chimeric USP to mediate a stage-specific function associated with the LBD.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"125-33"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Z/EG是一种双报告小鼠系，在cre介导的切除中表达增强的绿色荧光蛋白。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
A Novak, C Guo, W Yang, A Nagy, C G Lobe
{"title":"Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision.","authors":"A Novak,&nbsp;C Guo,&nbsp;W Yang,&nbsp;A Nagy,&nbsp;C G Lobe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Cre/loxP system has become an important tool in designing postintegrational switch mechanisms for transgenes in mice. The power and spectrum of application of this system depends on transgenic mouse lines that provide Cre recombinase activity with a defined cell type-, tissue-, or developmental stage-specificity. We have developed a novel mouse line that acts as a Cre reporter. The mice, designated Z/EG (lacZ/EGFP), express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to indicate the occurrence of Cre excision from early embryonic to adult lineages. The advantage of the Z/EG line is that Cre-mediated excision can be monitored in live samples and that live cells with Cre-mediated excision can be isolated using a single-step FACS. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"147-55"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts. 从神经细胞衍生克隆囊胚建立小鼠胚胎干细胞系。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
E Kawase, Y Yamazaki, T Yagi, R Yanagimachi, R A Pedersen
{"title":"Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts.","authors":"E Kawase,&nbsp;Y Yamazaki,&nbsp;T Yagi,&nbsp;R Yanagimachi,&nbsp;R A Pedersen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have established mouse embryonic stem (ES) cell lines from blastocysts derived by transfer of nuclei of fetal neuronal cells. These neuronal cell-derived embryonic cell lines had properties that characterize them as ES cells, including typical cell markers and alkaline phosphatase activity. Moreover, the cells had a normal karyotype and were pluripotent, as they were capable of differentiating into all three germ layers. Although they were derived from neuronal donor nuclei, the cells no longer expressed neuronal markers; however, they were capable of differentiating into cells with neuronal characteristics. These results suggest that the clone-derived cells have fully acquired an ES cell character. Thus, ES cells can be derived from embryos resulting from nuclear transfer, which results in reprogramming of the genetic information and acquisition of pluripotency. ES cells established from somatic cell-derived blastocysts could be useful not only as research tools for studying reprogramming but also as models for cell-based transplantation therapy.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"156-63"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21930488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Function of Rx, but not Pax6, is essential for the formation of retinal progenitor cells in mice. 小鼠视网膜祖细胞的形成离不开 Rx 而非 Pax6 的功能。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
L Zhang, P H Mathers, M Jamrich
{"title":"Function of Rx, but not Pax6, is essential for the formation of retinal progenitor cells in mice.","authors":"L Zhang, P H Mathers, M Jamrich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rx plays a critical role in eye formation. Targeted elimination of Rx results in embryos that do not develop eyes. In this study, we have investigated the expression of Otx2, Six3, and Pax6 in Rx deficient embryos. We find that these genes show normal activation in the anterior neural plate in Rx-/- embryos, but they are not upregulated in the area of the neural plate that would form the primordium of the optic vesicle. In contrast, in homozygous Small eye embryos that lack Pax6 function, Rx shows normal activation in the anterior neural plate and normal upregulation in the optic vesicle/retinal progenitor cells. This suggests that neither Rx expression nor the formation of retinal progenitor cells is dependent on a functional copy of the Pax6 gene, but that Pax6 expression and the formation of the progenitor cells of the optic cup is dependent on a functional copy of the Rx gene.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"135-42"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cre recombinase expression in cerebellar Purkinje cells. Cre重组酶在小脑浦肯野细胞中的表达。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
J J Barski, K Dethleffsen, M Meyer
{"title":"Cre recombinase expression in cerebellar Purkinje cells.","authors":"J J Barski,&nbsp;K Dethleffsen,&nbsp;M Meyer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cerebellar cortex and its sole output, the Purkinje cell, have been implicated in motor coordination, learning and cognitive functions. Therefore, the ability to generate Purkinje cell-specific mutations in physiologically relevant genes is of particular neurobiological interest. A suitable approach is the Cre/loxP strategy that allows temporally and spatially controlled gene inactivation. Here, we present the characterization of transgenic mouse strains expressing Cre recombinase controlled by the L7/pcp-2 gene. Endogenous L7/pcp-2 protein is expressed exclusively in Purkinje cells and retinal bipolar neurones. Recombination was detected by beta-galactosidase histochemistry in tissues from crosses of the L7/pcp-2:Cre transgenic lines with two different indicator strains, GtROSA26 and ACZL. Purkinje cells in all folia of the cerebellum displayed intense beta-galactosidase staining, whereas only few blue cells were observed in the retina and other parts of the CNS. Thus, these transgenic lines are potentially of great importance for genetic manipulations in cerebellar Purkinje cells.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 3-4","pages":"93-8"},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution and development at the National Science Foundation, U.S.A. 美国国家科学基金会的进化与发展
IF 1.5 4区 生物学
Genesis Pub Date : 2000-10-01
J Plesset, S Scheiner, S Singer
{"title":"Evolution and development at the National Science Foundation, U.S.A.","authors":"J Plesset,&nbsp;S Scheiner,&nbsp;S Singer","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"28 2","pages":"45-6"},"PeriodicalIF":1.5,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21892709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic analysis of the exed region in mouse. 小鼠外显区遗传分析。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-08-01 DOI: 10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w
S K Kendall, S J Strong, R T Litman, G W Litman, T Magnuson
{"title":"Genetic analysis of the exed region in mouse.","authors":"S K Kendall,&nbsp;S J Strong,&nbsp;R T Litman,&nbsp;G W Litman,&nbsp;T Magnuson","doi":"10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w","DOIUrl":"https://doi.org/10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w","url":null,"abstract":"<p><p>The extraembryonic ectoderm development (exed) mutant phenotype was described in mice homozygous for the c(6H) deletion, a radiation-induced deletion in the tyrosinase region of mouse Chromosome 7. These mutants fail to gastrulate and die around embryonic day 8.0. Several genes including, for example, embryonic ectoderm development (eed), are deleted in the c(6H) mutants; however, the portion of the chromosome responsible for the more severe exed phenotype is localized to a 20-kb region called the \"exed-critical region.\" To understand the genetics behind the exed phenotype, we analyzed this region in two ways. First, to determine whether the 20-kb exed-critical region alone causes the mutant phenotype, we removed it from a wild-type chromosome. The resulting mice homozygous for this deletion were viable and fertile, indicating that the 20-kb exed-critical region by itself is not sufficient to cause the phenotype when deleted. We then sequenced the 20-kb exed-critical region and no expressed exons were found. Several short matches to GenBank Expressed Sequence Tag (EST) databases were identified; however, none of these ESTs mapped to the region. Taken together, these results indicate that the exed phenotype may either be a position effect on a distal gene caused by the c(6H) breakpoint or the result of composite effects of nullizygosity of multiple genes in the deletion homozygotes.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"27 4","pages":"174-9"},"PeriodicalIF":1.5,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21827170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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