Genesis最新文献 - Book学术

Efficient Gene Expression System in Medaka Embryos Enables Functional Characterization of nt5c1a Paralogs Involved in Inosine Monophosphate Metabolism. Medaka胚胎中高效的基因表达系统使参与肌苷单磷酸代谢的nt5c1a同源物的功能表征成为可能。

IF 1.5 4区生物学

Genesis Pub Date : 2026-06-01 DOI: 10.1002/dvg.70056

Yu Murakami, Tomohisa Horibe, Masashi Ando, Toru Kobayashi

{"title":"Efficient Gene Expression System in Medaka Embryos Enables Functional Characterization of nt5c1a Paralogs Involved in Inosine Monophosphate Metabolism.","authors":"Yu Murakami, Tomohisa Horibe, Masashi Ando, Toru Kobayashi","doi":"10.1002/dvg.70056","DOIUrl":"10.1002/dvg.70056","url":null,"abstract":"Optimizing transient expression systems in fish embryos is crucial for rapid gene function analysis. Here, we established an efficient system in medaka (Oryzias latipes) embryos by evaluating nucleic acid type and injection site. Our results revealed that injecting the elongation factor 1αA (ef1αA) promoter-driven plasmid into the yolk yielded the highest expression on day 1 post-fertilization. Using this optimized system, we investigated cytosolic 5'-nucleotidase 1a (nt5c1a), which is involved in the metabolism of inosine monophosphate (IMP), an umami flavor compound. In silico analysis revealed that medaka had two nt5c1a paralogs: nt5c1aa and nt5c1ab. While nt5c1ab retains conserved substrate-recognition sequences and exhibits significant IMP degradation activity, nt5c1aa has lost these functions. Structural analysis using AlphaFold revealed that the Nt5c1aa L305P mutation causes local conformational changes near the substrate-binding site, potentially altering substrate orientation without disrupting the overall protein fold. Our expression system demonstrated that this single L305P substitution partially restored IMP-degrading activity in Nt5c1aa, confirming that residue 305 is a key determinant of its functional divergence. Our findings provide a robust foundation for molecular breeding to enhance umami flavor in farmed fish. Specifically, the targeted manipulation of these nt5c1a paralogs could facilitate developing breeds with maximized IMP accumulation in muscle tissues.","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"64 3","pages":"e70056"},"PeriodicalIF":1.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Comparison and Splice Site Conservation of the Animal SMNDC1 Gene Family. 动物SMNDC1基因家族的系统发育比较及剪接位点保护。

IF 1.5 4区生物学

Genesis Pub Date : 2026-06-01 DOI: 10.1002/dvg.70054

Jing-Wan Zhang, Hong-Mei Wang, Guojun Ouyang, Chao Sun, Bao-Xing Huang

{"title":"Phylogenetic Comparison and Splice Site Conservation of the Animal SMNDC1 Gene Family.","authors":"Jing-Wan Zhang, Hong-Mei Wang, Guojun Ouyang, Chao Sun, Bao-Xing Huang","doi":"10.1002/dvg.70054","DOIUrl":"10.1002/dvg.70054","url":null,"abstract":"Alternative splicing is a key post-transcriptional process that generates multiple mRNAs from a single pre-mRNA through the coordinated action of the spliceosome and associated splicing factors. SMNDC1 (survival motor neuron domain containing 1) has been identified as an integral component of the spliceosome complex. Previous studies have shown that SMNDC1 is essential for splicing catalysis in vitro and plays a regulatory role in intron retention in cancer. However, the phylogenetic relationships and expression patterns of SMNDC1 across the animal kingdom have not been systematically investigated. In this study, we conducted a comprehensive phylogenetic analysis of SMNDC1 genes in animals. A total of 72 SMNDC1 genes were identified from 66 animal species. Bioinformatic analyses revealed that the gene structure and protein domains of SMNDC1 are highly conserved, with only a few species possessing gene duplications. Notably, the human SMNDC1 gene exhibits elevated expression levels in multiple cancer types, including breast, colon, and rectal cancers, suggesting its potential role in tumor development and its value as a diagnostic or therapeutic target. Overall, our findings provide a systematic overview of the SMNDC1 gene family in animals and establish a foundation for future studies exploring its molecular functions and evolutionary significance.","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"64 3","pages":"e70054"},"PeriodicalIF":1.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147876279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pvr and Pvf2 Are Essential for Valve Cell Differentiation in the Larval Drosophila Heart. Pvr和Pvf2对果蝇幼体心脏瓣膜细胞分化至关重要。

IF 1.5 4区生物学

Genesis Pub Date : 2026-06-01 DOI: 10.1002/dvg.70053

Heiko Meyer, Christian Meyer, Bettina Priess, Achim Paululat

引用次数: 0

Ectopic Expression of T-Box Transcription Factors Alters Mouse Forelimb Formation. T-Box转录因子的异位表达改变小鼠前肢形成。

IF 1.5 4区生物学

Genesis Pub Date : 2026-04-01 DOI: 10.1002/dvg.70050

Mariah H Denhart, Deborah L Chapman

{"title":"Ectopic Expression of T-Box Transcription Factors Alters Mouse Forelimb Formation.","authors":"Mariah H Denhart, Deborah L Chapman","doi":"10.1002/dvg.70050","DOIUrl":"10.1002/dvg.70050","url":null,"abstract":"The developing vertebrate forelimb expresses seven T-box transcription factors, with several in overlapping expression domains. All T-box transcription family members share similarity within their DNA binding domain, the T-domain. Outside of the T-domain, these factors share little similarity, allowing family members to have different transcriptional properties and binding partners. Several human T-box genes show haploinsufficiency in the limb, including Tbx5 and Tbx3 that, when mutated, cause Holt-Oram and ulnar-mammary syndrome, respectively. This dosage sensitivity combined with the shared T-domain leads to our hypothesis that when co-expressed a competition between T-box factors at target genes can occur. To test this, we ectopically expressed two exogenous T-box factors, T and Tbx6, in the developing forelimb mesenchyme to examine how artificially changing the relative levels of T-box proteins affects forelimb formation. Skeletal, apoptotic, and gene expression assays were used to characterize the resulting phenotypes. While ectopic T and Tbx6 both affected the size and shape of the forearm bones and ossification, they differentially affected digit formation: T caused loss of digits and Tbx6 led to phalange bone duplications and extra digit formation. These dissimilar phenotypes suggest that these transcriptional activators differentially affect pathways critical for regulating forelimb development.","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"64 2","pages":"e70050"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13080222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bibliographie : études génétiques, éditions, manuscrits 参考书目:遗传学研究，版本，手稿

4区生物学

Genesis Pub Date : 2023-10-01 DOI: 10.4000/genesis.8501

Giovanni Zuccarino

引用次数: 0

Kathryn Sutherland, Why Modern Manuscripts Matter, Oxford, Oxford University Press, 2022, 273 p. 凯瑟琳·萨瑟兰，为什么现代手稿很重要，牛津，牛津大学出版社，2022年，273页。

4区生物学

Genesis Pub Date : 2023-10-01 DOI: 10.4000/genesis.8567

Flavie Épié

引用次数: 0

Violente splendeur de « Rêves » “梦想”的暴力辉煌