IF 1.5 4区生物学

Genesis Pub Date : 2000-01-01 DOI: 10.1002/(sici)1526-968x(200001)26:1<67::aid-gene9>3.3.co;2-u

J Wang, F Tie, E Jane, A Schumacher, P J Harte, T Magnuson

引用次数: 0

LMX1B, a LIM homeodomain class transcription factor, is necessary for normal development of multiple tissues in the anterior segment of the murine eye. LMX1B是一种LIM同源结构域类转录因子，是小鼠眼前段多个组织正常发育所必需的。

IF 1.5 4区生物学

Genesis Pub Date : 2000-01-01

C L Pressman, H Chen, R L Johnson

{"title":"LMX1B, a LIM homeodomain class transcription factor, is necessary for normal development of multiple tissues in the anterior segment of the murine eye.","authors":"C L Pressman, H Chen, R L Johnson","doi":"","DOIUrl":"","url":null,"abstract":"Proper development of the anterior segment of the mammalian eye is critical for normal ocular function. Indeed, several congenital syndromes associated with anterior segment anomalies can lead to impaired vision and glaucoma. One such syndrome is nail patella syndrome (NPS), caused by haploinsufficiency for the LIM-homeodomain transcription factor LMX1B. Although mutations in LMX1B cosegregate with NPS, whether these mutations cause the glaucoma associated with NPS is not known. Here, we provide evidence that the LIM-homeodomain transcription factor lmx1b is an essential regulator of murine anterior segment development. Mice that are homozygous for a targeted mutation of lmx1b display iris and ciliary body hypoplasia, and cornea stromal defects. In addition, two cDNAs normally downregulated in presumptive cornea, mf1 and mfh1, exhibit persistent expression, while keratocan, a keratin sulfate proteoglycan expressed by keratocytes, is not detected in mutant corneas. Moreover, ultrastructural examination of homozygous mutants indicates that corneal collagen fibrillogenesis is perturbed. Taken together, our studies suggest a developmental etiology for glaucoma in NPS patients and highlight lmx1b as an essential regulator of anterior segment morphogenesis and patterning. genesis 26:15-25, 2000.","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"26 1","pages":"15-25"},"PeriodicalIF":1.5,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21515407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac enhancer activity of the homeobox gene tinman depends on CREB consensus binding sites in Drosophila. 同源盒基因tinman的心脏增强子活性取决于果蝇的CREB共识结合位点。

IF 1.5 4区生物学

Genesis Pub Date : 2000-01-01

T V Venkatesh, M Park, K Ocorr, J Nemaceck, K Golden, M Wemple, R Bodmer

{"title":"Cardiac enhancer activity of the homeobox gene tinman depends on CREB consensus binding sites in Drosophila.","authors":"T V Venkatesh, M Park, K Ocorr, J Nemaceck, K Golden, M Wemple, R Bodmer","doi":"","DOIUrl":"","url":null,"abstract":"The Drosophila homeobox gene tinman plays a critical role in subdividing the early mesoderm. In particular, tinman is absolutely required for formation of the heart and visceral mesoderm. tinman expression is initiated throughout the mesoderm of the trunk region under the control of the bHLH transcription factor encoded by the twist gene, a determinant of all mesoderm. Later, tinman expression is restricted to the dorsal portion of the mesoderm, a process that is directed by decapentaplegic (dpp) whose product (a TGF-beta-related protein) is secreted by the overlaying ectoderm. Further restriction of tinman expression to the cardiac progenitors, in which it will persist throughout development, involves the secreted segmentation gene product encoded by wingless (wg, a Drosophila Wnt gene). Here, we show that strong early expression depends on the synergistic action of an enhancer element at the 5' end of the gene in conjunction with an element in the first intron. Moreover, two distinct enhancer regions are responsible for tinman expression in the heart: one region confers expression in the heart-tube-associated pericardial cells, the other element drives expression in the contractile, myocardial cells. The latter element contains two CREB consensus binding sites that are essential for cardiac-specific expression. genesis 26:55-66, 2000.","PeriodicalId":48923,"journal":{"name":"Genesis","volume":"26 1","pages":"55-66"},"PeriodicalIF":1.5,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21515333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneous populations of ES cells in the generation of a floxed Presenilin-1 allele. 早老素-1等位基因产生的胚胎干细胞异质群体。

IF 1.5 4区生物学

Genesis Pub Date : 2000-01-01

H Yu, J Kessler, J Shen

引用次数: 0

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