Biomass Conversion and Biorefinery最新文献

{"title":"Comparative analysis of N2 and H2 atmospheres in fast pyrolysis of mixed palm waste: Optimizing deoxygenation and hydrocarbon yield","authors":"Ali Reza Aghamiri,&nbsp;Pooya Lahijani,&nbsp;Abdul Rahman Mohamed,&nbsp;Keat Teong Lee,&nbsp;Farzad Ismail","doi":"10.1007/s13399-024-06250-5","DOIUrl":"10.1007/s13399-024-06250-5","url":null,"abstract":"<div><p>The utilization of biofuel derived from inexpensive, non-edible oils is a promising alternative to conventional fuels. This study focuses on the use of palm waste (MPW), including Palm Fiber (PF), Empty Fruit Bunch (EFB), and Palm Kernel Shell (PKS), as feedstock for biofuel production through fast pyrolysis. It examines the effects of temperature and gas flow in hydrogen and nitrogen atmospheres, aiming to optimize bio-oil yield, hydrocarbon content, and deoxygenation to produce bio-oil with properties close to conventional fuels, making it more suitable for biofuel applications. Bio-oil samples were characterized using gas chromatography-mass spectrometry (GC-MS) to assess their chemical composition. The results show that although N₂ (36.6%) at the optimum temperature of 600 °C and a flow rate of 300 ml/min can produce a higher liquid yield compared to H₂ (34.1) during fast pyrolysis, fast pyrolysis in H₂ can produce a liquid with a higher hydrocarbon, ester, and alcohol content, and improved deoxygenation. Pyrolysis in a hydrogen environment showed better performance in producing less oxygenated bio-oil with better comparative components. To optimize bio-oil quality, this study employed four indices using the Design Expert Response Surface Methodology (RSM): bio-oil yield, hydrocarbon yield, molecular chains with more than five carbons, and deoxygenation. The optimal conditions for H₂ (473^oC and 500 ml/min) were identified to maximize these indices. The experimental results showed a bio-oil yield of 27.45%, a hydrocarbon yield of 9.21%, 91.39% molecular chains with more than five carbons, and a deoxygenation rate of 69.46%, which aligned well with the predictions from the Central Composite Design (CCD) method.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"15161 - 15179"},"PeriodicalIF":3.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring chemically processed Symplocos racemosa sustainable material feasibility for sorptive amputation of methylene blue dye from waste water by green technology","authors":"Muhammad Rashid,&nbsp;Rabia Rehman,&nbsp;Mehwish Akram,&nbsp;Ayman A. Ghfar,&nbsp;Liviu Mitu","doi":"10.1007/s13399-024-06219-4","DOIUrl":"10.1007/s13399-024-06219-4","url":null,"abstract":"<div><p>In this work, <i>Symplocos racemosa</i> (Lodh) biowaste (SR) was chemically fabricated to make it a sustainable material for detoxifying cationic pollutants from waste-water by green technology using methylene blue (MB) dye as a test case. Morphological changes that occurred in SR were observed by SEM, EDX, XRD, and FTIR methods. Batch adsorption experiments were conducted to optimize tailored adsorptive detoxification of the MB dye. The equilibrium data of experiments was found to be in best fit with Langmuir isotherm, showing that the maximum dye removal capacity of SR (q_max) was 10 mg/g for MB. Kinetic modeling of equilibrium data followed pseudo-second-order kinetics, indicating enhanced porosity after chemical processing of SR. The results confirmed the prospective application of <i>Symplocos racemosa</i> lignocellulosic waste as a natural, cheap, and sustainable product that can be used for adsorptive removal of cationic pollutants.\u0000</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13609 - 13626"},"PeriodicalIF":3.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new green catalyst and antimicrobial agent derived from eco-friendly products of camel bones: synthesis and physicochemical characterization","authors":"Amr Mohammad Nassar,&nbsp;Wael A. A. Arafa,&nbsp;Khulaif Ashammari,&nbsp;Shaima M. N. Moustafa,&nbsp;Alaa Muqbil Alsirhani,&nbsp;M. F. Hasaneen","doi":"10.1007/s13399-024-06234-5","DOIUrl":"10.1007/s13399-024-06234-5","url":null,"abstract":"<div><p>The circular economy, which aims to produce sustainable materials from waste, plays an important role in environmental research. This work’s primary goal is to reuse food waste to synthesize usable materials. We describe here a sustainable, green, easy, fast, and cheap method for recycling waste camel bone as a renewable material for effective applications. The recycled camel bone (RCB) material has been characterized using thermal gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR, ultraviolet–visible absorption spectra (UV–Vis), energy band gap, X-ray diffraction (XRD), scanning electron microscopy (SEM)), and Raman spectroscopy were used to analyze the produced material. Particle sizes in the range of 100–120 nm were seen in SEM images. RCB has been utilized as a robust heterogeneous catalyst to promote Knoevenagel condensation reactions in an aqueous medium. The applied sustainable conditions promoted the reactions to afford superior yields (≈98%) after 8 min for all derivatives with six cycles without remarkable alterations in activity. The antimicrobial activity of camel bones, extracted organic species, and extracted hydroxyapatite was assessed against pathogenic microbes: <i>Bacillus cereus</i>, <i>Enterococcus faecium</i>, <i>Escherichia coli</i>, and <i>Candida albicans</i>. Hydroxyapatite showed the best antimicrobial activity against the studied microbial strains.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13589 - 13607"},"PeriodicalIF":3.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of laccase production by Pleurotus pulmonarius through solid substrate fermentation of tender coconut fiber: enhanced laccase production and biomass delignification","authors":"Geethu Suresh,&nbsp;Ragunathan R,&nbsp;Jesteena Johney","doi":"10.1007/s13399-024-06263-0","DOIUrl":"10.1007/s13399-024-06263-0","url":null,"abstract":"<div><p>Tender coconut fiber, an abundant agro-waste, requires chemical or biological pretreatment to enhance its potential for value-added applications due to its complex lignocellulosic composition. Pretreatment of tender coconut fiber with laccase-producing white rot fungi presents a sustainable strategy for effective delignification and waste management. This study investigated the biological pretreatment of tender coconut fiber using the white rot fungus, <i>Pleurotus pulmonarius</i>, for delignification through enhanced laccase production via solid-state fermentation. This research involved exploring the influence of different factors on laccase production and understanding the relationships between these factors through response surface methodology (RSM) to obtain the maximum laccase production. A central composite design was employed to optimize the process parameters, including pH, temperature, incubation time, and concentration of corn steep liquor (CSL). The optimized process parameters were a pH of 5.6, a temperature of 27 °C, an incubation time of 21 days, and a CSL concentration of 3%. This optimization resulted in a remarkable increase in enzyme activity, reaching 663.76 ± 10.67 IU/ml, indicating a substantial 13.16-fold increase after the optimization process. Scanning electron microscopy (SEM) and FTIR analysis were performed to observe the changes in the surface structures of the raw and delignified fiber. The enhanced laccase activity suggests the potential for tender coconut fiber to be used to produce laccase enzymes, which has not been explored before. This approach offers a solution for managing tender coconut fiber and unlocks the potential for valorization due to the enhanced properties of the modified fiber.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13769 - 13781"},"PeriodicalIF":3.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green technologies for production of microbial bioplastics from agricultural biowaste: a review","authors":"Purnima Kumari,&nbsp;Sheetal Mane,&nbsp;Anupama Singh,&nbsp;Komal Chauhan,&nbsp;Neetu K. Taneja","doi":"10.1007/s13399-024-06249-y","DOIUrl":"10.1007/s13399-024-06249-y","url":null,"abstract":"<div><p>The substantial increase in greenhouse gas (GHG) emissions from industrialization and urbanization is the primary driver of climate change, presenting significant environmental and health hazards on a global scale. One major contributor to GHG emissions is the production and disposal of plastics, which are ubiquitous in modern life. Plastics, primarily used in packaging, pose significant environmental and health risks owing to their non-renewable nature. To mitigate these impacts, polyhydroxybutyrate (PHB), a bioplastic derived from microorganisms, has gained attention as a sustainable alternative. One effective method for PHB production is the utilization of agro bio-waste as a carbon source through bacterial fermentation. Additionally, there are various factors, such as nutrient availability, bacterial strains, and fermentation conditions, which influence the efficiency of PHB production. To further enhance the PHB production efficiency, novel technologies such as microwave and ultrasonic-assisted treatments are being explored for their ability to breakdown complex sugars in bio-waste into fermentable sugars. These interventions offer advantages over traditional methods, such as reduced energy consumption and minimal use of harmful chemicals and expensive enzymes. By utilizing agro bio-waste as feedstock for PHB production, not only can environmental concerns associated with traditional plastics be addressed, but the sustainability of the packaging industry can also be promoted. This review aims to provide an overview of microbial and technological interventions in PHB production from agro bio-waste, emphasizing their potential to promote sustainability and reduce plastic pollution.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13143 - 13163"},"PeriodicalIF":3.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on fatigue, creep, and tribological performance of coconut shell, seashell, and eggshell filler-based bio-fiber-reinforced epoxy hybrid composites","authors":"Bodhisatwa Seal,&nbsp;Vijay Chaudhary,&nbsp;Susmita Dey Sadhu","doi":"10.1007/s13399-024-06266-x","DOIUrl":"10.1007/s13399-024-06266-x","url":null,"abstract":"<div><p>Environmental awareness against synthetic plastics focused on the bio resources (natural fibers, fillers, and bio-resins) for the development of biodegradable composite materials. In the present study, bio nano fillers (seashell, eggshell, and coconut) and bio-fibers (pineapple, sisal, and kenaf) were reinforced with epoxy polymer matrix to develop the bio composite materials. Tribological testing was performed with input parameters (applied load, sliding speed, and sliding distance) and out parameters were recorded in terms of frictional force, coefficient of friction (COF), and specific wear rate (SWR). Fatigue and creep analysis of all prepared composites were performed to analyze the strength of developed composite specimens during fluctuating and static load conditions. Experimental finding of fatigue test reveals that KES composite achieved maximum number of fatigue cycles of 4460, 3250, and 2210 at 25, 50, and 75% ultimate tensile strength (UTS). Creep strain was maximum in SE composite from time 0 to 15,000 s. Frictional force was maximum by PEC and KEC composite of 7.68 N and 1.14 N at 30 N applied load and sliding distance of 1.83. Maximum and minimum COF was achieved by KEC composite and hybrid SE composite of 0.25 and 0.07 at 500 RPM and 10 N load. At 500 RPM and 10 N load, PEE composite achieved maximum SWR of 82 mm³/N-mm and minimum was 5.38 mm³/N-mm by SES composite. Hybrid PSKE composite archived maximum interfacial temperature of 26 °C and minimum was 17 °C by SEE composite at 30 N applied load and 500 RPM.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"14881 - 14895"},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fuel characterization and gasification of sunflower waste in sub- and supercritical water with influence of catalysts on optimum state","authors":"Hamit Türkmen,&nbsp;İbrahim Diker,&nbsp;Ender Fakı,&nbsp;Hüseyin Akilli","doi":"10.1007/s13399-024-06254-1","DOIUrl":"10.1007/s13399-024-06254-1","url":null,"abstract":"<div><p>The amounts of H₂ production through subcritical water gasification (Sub-CWG) and supercritical water gasification (Super-CWG) have been revealed in the current study. Sunflower waste (SW) was selected as the biomass used in the gasification processes. To determine the fuel characteristics of SW, proximate, ultimate, and higher heating value (HHV) analyses were conducted. The response surface methodology (RSM) was applied to design the experimental runs, to determine and optimize the process parameters, and to explore the interactions between them, which included reaction temperature, feed concentration, and residence time. Furthermore, various catalyst additives (K₂CO₃, Na₂CO₃, and NaOH) were used at the optimum level of process parameters to investigate the effect of catalyst on H₂ production. According to the results of gasification processes, the amount of H₂ production in Sub-CWG was lower when compared to Super-CWG and the main gas yield was CO₂ and CH₄ due to low reaction temperature and residence time. On the other hand, it has been revealed that Super-CWG is much more efficient in H₂ production. Elevated temperatures and extended residence times enhance H₂ production by facilitating key reactions such as the water–gas shift and steam reforming. Higher feed concentrations were found to reduce H₂ production due to dilution effects and reduced water availability. The RSM explored that the created model matched the experimental data in H₂ production since the correlation coefficient values were high enough (<i>R</i>² = 99.83%, <i>R</i>²_Adj = 99.52%). In the second part of the study, catalytic Super-CWG experiments were conducted using the optimum process parameters determined by RSM. According to the results, NaOH significantly improves H₂ production by enhancing C–C bond cleavage and promoting the water–gas shift reaction, and the mechanistic basis for catalytic activity lies in the reduction of CO and CO₂ formation, thus maximizing H₂ production.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"15327 - 15340"},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of progress on torrefaction, pyrolysis and briquetting of banana plant wastes for biofuels","authors":"Emmanuel Menya,&nbsp;Collins Okello,&nbsp;Henning Storz,&nbsp;Joel Wakatuntu,&nbsp;Medard Turyasingura,&nbsp;David K. Okot,&nbsp;Simon Kizito,&nbsp;Allan John Komakech,&nbsp;Isa Kabenge,&nbsp;Samson Rwahwire,&nbsp;Peter Wilberforce Olupot","doi":"10.1007/s13399-024-06204-x","DOIUrl":"10.1007/s13399-024-06204-x","url":null,"abstract":"<div><p>The banana value chain produces over 4 tonnes of waste biomass for every tonne of bananas harvested, including leaves, pseudostems, peels, rejected fruits, rhizomes, and empty fruit bunches. With rising fossil fuel costs and environmental concerns, these wastes present opportunities for alternative biofuel production through thermochemical processing and densification. This review examines the properties of various banana plant wastes, their pretreatments, and suitability for processes like pyrolysis, torrefaction, and hydrothermal carbonization, as well as briquetting. Banana plant wastes vary in physico-chemical properties depending on the biomass type. Their high volatile matter content (70.5–89.1%db) makes them better suited for bio-oil and gas production rather than biochar. Pretreatment methods such as water-washing, alkaline treatment, drying, pressing, chopping, grinding, and milling may be needed before thermochemical conversion of the wastes. Among conversion routes, pyrolysis is the most studied, followed by hydrothermal carbonization and dry torrefaction. The hydraulic press is the most commonly used technology for briquetting banana plant wastes. Depending on factors such as binder-to-biomass ratio, dwell time, and compaction pressure, this method can produce briquettes with compressive strength ranging from 1.33 to 38.39 MPa, which exceeds the minimum acceptable level of 0.38 MPa. However, these briquettes can have ash content as high as &gt; 20%db, which can reduce their calorific value, increase the risk of ash slagging and fouling in combustion systems, as well as lead to increased emission of particulate matter during combustion. While thermochemical conversion and briquetting of banana plant wastes may incur significant costs, these could be offset by the low cost of the raw materials, improved fuel properties, and better handling, transportation, and storage. Research efforts should focus on ascertaining the emission potential of thermochemical conversion and briquetting of banana plant wastes, which could encourage wider acceptance of these technologies, especially considering growing awareness about the need for environmental protection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 9","pages":"13227 - 13269"},"PeriodicalIF":3.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobiocatalysts for efficient conversion of microwave aided ionic liquid pretreated rice straw biomass to biofuel","authors":"Arpana Thakur,&nbsp;Surbhi Sharma,&nbsp;Taniya Khajuria,&nbsp;Muskaan Chib,&nbsp;Ridhika Bangotra,&nbsp;Nisha Kapoor,&nbsp;Ritu Mahajan,&nbsp;Bijender Kumar Bajaj","doi":"10.1007/s13399-024-06236-3","DOIUrl":"10.1007/s13399-024-06236-3","url":null,"abstract":"<div><p>Growing energy demand, devastating effects of excess fossil fuel usage and its declining reserves, and grave environmental/climate change issues are the major motivational points for developing renewable, sustainable, safer, and green energy alternatives. The current research examined the rice straw biomass (RSB) as the potential feedstock for producing biofuel-ethanol. RSB was subjected to a sequential pretreatment with ionic liquid tris (2-hydroxyethyl) methylammonium methyl sulphate ([TMA][MeSO₄]) and microwave irradiation, and the pretreated RSB was hydrolyzed with either free cellulase/xylanase enzyme preparation, or the one immobilized on magnetic nanoparticles (MNPs), i.e., nanobiocatalysts, to achieve a sugar content, respectively, of 159.77 mg/g biomass and 157.03 mg/g biomass. Sugar hydrolysate obtained from free or nanobiocatalysts mediated hydrolysis was fermented to bioethanol content of 78.48 mg/g and 78.05 mg/g biomass, respectively. An ionic liquid stable cellulase/xylanase enzyme preparation was <i>in-house</i> developed by submerged fermentation from <i>Aspergillus niger</i> B4. Nanobiocatalysts exhibited significant reusability potential for several successive hydrolysis cycles. The structural alterations in the pretreated biomass were elucidated by XRD, FTIR, and SEM analyses. MNPs and nanobiocatalysts were examined by VSM, SEM, DLS, TG–DTA, and FTIR analysis for structural/functional/operational characteristics. The current study is the first ever report of combined pretreatment of RSB using IL ([TMA][MeSO₄]) and microwave irradiation followed by saccharification with IL-stable, <i>in-house</i> developed cellulase/xylanase enzymes from <i>Aspergillus niger</i> B4. The study unravels new avenues for economic and eco-benign bioconversion of rice straw biomass into biofuel-ethanol.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"15123 - 15140"},"PeriodicalIF":3.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid extract with high amino nitrogen obtained by autolysis of brewing yeast can be used as supplement for bioethanol production","authors":"Rodrigo J. Leonardi,&nbsp;Sofía Racca,&nbsp;Raúl N. Comelli,&nbsp;Lisandro G. Seluy","doi":"10.1007/s13399-024-06241-6","DOIUrl":"10.1007/s13399-024-06241-6","url":null,"abstract":"<div><p>Brewer’s spent yeast (BSY) is a commonly underutilized by-product of the brewing industry with a highly negative environmental impact. One of the methods to add value and reduce environmental footprint is the generation of liquid yeast extracts intended for formulating culture media for the propagation of microorganisms of biotechnological and agro-industrial interest. Autolysis is the most commonly employed non-mechanical process for the industrial production of yeast extracts. Prior to the scale-up valorisation of BSY, optimization of the physicochemical operational conditions of autolysis is required, as these factors can significantly impact the yields and composition of the autolysate. In the present study, we investigated the effects of temperature (40–50 °C), initial pH (3–8), and different pH control systems using autolysis buffers, on the release of solids and assimilable nitrogen content of the yeast <i>Saccharomyces cerevisiae</i> SafAle™ S-04, widely used by industrial and craft breweries in Argentina and worldwide. Our results allowed to analyse and compare a large number of experimental variables and find optimal conditions for autolysis performance. Autolysis carried out at 40 °C and pH 4 effectively facilitates the recovery of a high percentage of free amino nitrogen and autolysate was able to successfully support the fermentation process of <i>S. cerevisiae</i> var. Ethanol Red®, in the presence of a high initial concentration of sugar, exhibiting highly similar ethanol yield compared to a commercial yeast extract. Liquid autolysates offer adequate nutritional quality for designing culture media for microorganism cultivation and facilitating various biotechnological processes.</p></div>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":"15 10","pages":"15141 - 15159"},"PeriodicalIF":3.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}