Journal of Oral & Facial Pain and Headache最新文献

{"title":"Targeted online health information was associated with more severe temporomandibular disorders.","authors":"Yijun Li, Li Zhang, Zhiwei Cao, Yanyu Sun, Ziang Xu, Carolina Marpaung, Jun Wang, Xin Xiong","doi":"10.22514/jofph.2025.081","DOIUrl":"10.22514/jofph.2025.081","url":null,"abstract":"<p><strong>Background: </strong>Temporomandibular disorders (TMDs) are typical biopsychosocial conditions often accompanied by anxiety, somatization and even cyberchondria. Targeted online health information is increasingly prominent in digital era yet its psychological impact on TMDs remains underexplored. To examine the association between targeted online health information and TMDs severity, and to explore whether anxiety, cyberchondria and somatization mediate this relationship.</p><p><strong>Methods: </strong>Participants were evaluated using questionnaires including the five TMDs symptoms (TMDs-5T) scale, 7-item Generalised Anxiety Disorder scale (GAD-7), short-form version of the cyberchondria severity scale (CSS-12) and somatic symptom scale-8 (SSS-8). Data on targeted online health information, search frequency and perceived impact were also collected. Analyses were conducted using SPSS and Mplus.</p><p><strong>Results: </strong>A total of 588 valid responses were analyzed. Greater TMDs severity was significantly associated with targeted online health information delivery (β = 1.373, <i>p</i> < 0.001), anxiety (β = 1.714, <i>p</i> < 0.001) and cyberchondria (β = 1.641, <i>p</i> < 0.001). The chain mediation model revealed that both the total effect and direct effect (Targeted online health information → TMDs-5T) were significant (β = 2.261, <i>p</i> < 0.001; β = 1.003, <i>p</i> < 0.001). A significant indirect pathway was also identified, in which targeted online health information influenced TMD severity through somatization, anxiety, and cyberchondria (β = 0.210, <i>p</i> = 0.026).</p><p><strong>Conclusions: </strong>Exposure to targeted online health information was associated with greater TMD symptom severity, mediated by psychological factors such as somatization, anxiety, and cyberchondria. These findings underscore the importance of algorithmic ethics, policy oversight, and user education to mitigate psychological risks in digital health environments.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"242-251"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of the clinical efficacy of GON block at the C2 level and GON block at the classical distal occipital level in the treatment of cluster headache.","authors":"Anıl Kılınç, Mustafa Karaoğlan","doi":"10.22514/jofph.2025.080","DOIUrl":"10.22514/jofph.2025.080","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to compare the clinical efficacy of C2-level and distal greater occipital nerve (GON) blockade in the management of cluster headache (CH), focusing on pain relief onset and overall treatment outcomes.</p><p><strong>Methods: </strong>In this retrospective study, 48 patients diagnosed with CH were divided into two equal groups: one receiving bilateral C2 GON blocks and the other undergoing bilateral distal GON blocks. Both groups were treated with dexamethasone as the steroid and bupivacaine as the local anaesthetic. Pain relief and treatment duration were assessed, and adverse events were monitored.</p><p><strong>Results: </strong>Both GON blockade techniques were effective in reducing CH symptoms. Patients treated with C2 GON blocks achieved complete pain relief by the third week, allowing for treatment cessation. In contrast, the distal GON block group required up to six injections for similar levels of relief. Rapid pain control was more pronounced in the C2 GON group, with no significant adverse effects observed in either group.</p><p><strong>Conclusions: </strong>C2 GON blockade demonstrated greater efficacy for early pain relief in CH patients compared with distal GON blockade. These findings suggest that C2 GON blockade may be a more suitable option for both transitional and preventive therapy in CH. Further studies are recommended to confirm these results and to explore the long-term benefits of C2 GON blockade in CH management.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"235-241"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of intraarticular drug injection in patients with temporomandibular joint disorders with limited mouth opening: a system review and network meta-analysis.","authors":"Siyu Zou, Yue Yue, Xinyu Wang, Qinglan Liu","doi":"10.22514/jofph.2025.041","DOIUrl":"10.22514/jofph.2025.041","url":null,"abstract":"<p><strong>Background: </strong>This network meta-analysis (NMA) aims to evaluate the comparative efficacy of 13 intra-articular pharmacologic interventions in improving maximal mouth opening (MMO), pain relief, and functional recovery in patients with temporomandibular disorders (TMD) and restricted jaw mobility.</p><p><strong>Methods: </strong>A comprehensive literature search was conducted in PubMed, Embase, Cochrane Library, and Web of Science to identify randomized controlled trials (RCTS) evaluating the effects of 13 intra-articular drug injections in patients with limited mouth opening TMD. The methodological quality of the included studies was assessed using the risk of bias (ROB) tool, and data were independently extracted by two researchers. Primary outcomes included maximum mouth opening (MMO) and pain intensity; secondary outcomes included joint lateral and protrusive movements.</p><p><strong>Results: </strong>A total of 38 RCTs involving 1533 TMD patients were included. Thirteen different agents (including HA: hyaluronic acid, PRF: platelet-rich fibrin, PDGF: platelet-derived growth factor, PRP: platelet-rich plasma, MOR: morphine, LA: local anesthetic, MA: micro-fragment fat, TRA: tramadol, SAL: Saline, GC: glucocorticoid, GLU: glucose, NS: non-steroidal, and ARTH: arthrocentesis only) were evaluated. NMA results showed that PRF injection after arthrocentesis significantly improved the maximum temporomandibular joint opening (surface under the cumulative ranking curve (SUCRA): 99.1%) compared with arthrocentesis alone. MA injection following arthrocentesis was most effective for pain reduction (SUCRA: 84.7%), followed by PRF (SUCRA: 78.2%). PRF also led to significant improvements in lateral jaw movement (SUCRA: 95.5%) and protrusive movement (SUCRA: 63.5%) compared to arthrocentesis alone.</p><p><strong>Conclusions: </strong>Based on the network ranking chart, PRF injection after arthrocentesis offers the greatest benefits for functional recovery in TMD patients. However, additional rigorous literature is required to validate this assertion.</p><p><strong>Clinical trial registration: </strong>Registration number is INPLASY202450107.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"85-99"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic factors identification using machine learning in temporomandibular disorder treatment responders.","authors":"Chollada Chamnanmanoontham, Thanaphon Tangchoopong, Jarin Paphangkorakit, Supanigar Ruangsri, Teekayu P Jorns","doi":"10.22514/jofph.2025.076","DOIUrl":"10.22514/jofph.2025.076","url":null,"abstract":"<p><strong>Background: </strong>Temporomandibular disorders (TMD) are complex chronic conditions that significantly impair quality of life and impose a considerable social and economic burden. Although various treatment strategies have been developed, the prognostic factors influencing therapeutic outcomes remain poorly defined. This study aimed to identify relevant prognostic factors for treatment response using machine learning methods, with patient readiness for discharge serving as the primary outcome.</p><p><strong>Methods: </strong>A total of 1050 medical records from patients diagnosed with TMD and treated at the Orofacial Pain and Dental Sleep Medicine clinic, between January 2018 and June 2023, were retrospectively analyzed with a follow-up period of one year. Twenty-six clinical and demographic variables were initially extracted and preprocessed using one-hot encoding. After the removal of highly correlated variables, the final dataset comprised 36 features derived from encoded categorical variables. Seven machine learning algorithms, namely Decision Tree, Random Forest, Logistic Regression, K-Nearest Neighbors (KNN), Support Vector Classifier (SVC), Gradient Boosting, and Extreme Gradient Boosting (XGBoost), were trained to predict discharge readiness. Model performance was evaluated using accuracy, precision, recall, F1 score, and the receiver operating characteristic-area under the curve (ROC-AUC). Feature importance was assessed using information gain and Shapley Additive exPlanations (SHAP) to interpret model predictions.</p><p><strong>Results: </strong>The Random Forest model demonstrated superior performance in predicting readiness for discharge, with an accuracy of 0.7901, precision of 0.8611, recall of 0.8943, F1 score of 0.7901, and ROC-AUC of 0.6442. SHAP analysis identified onset duration, pain severity, anxiety level, presence of neck pain, and body mass index (BMI) as the most influential predictors.</p><p><strong>Conclusions: </strong>The identified prognostic factors highlight the multidimensional nature of TMD and support their relevance in guiding patient-specific management strategies. The integration of machine learning approaches may enhance clinical decision-making and contribute to the development of more personalized and effective treatment pathways for TMD.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"190-206"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synovial chondromatosis of the temporomandibular joint: a case report with an associated systematic review update of the literature.","authors":"Ovidiu Ionut Saracutu, Matteo Val, Luca Guarda-Nardini, Marco Ferrari, Matteo Pollis, Daniele Manfredini","doi":"10.22514/jofph.2025.068","DOIUrl":"10.22514/jofph.2025.068","url":null,"abstract":"<p><strong>Background: </strong>This study aims to report a case of synovial chondromatosis (SC) of the left temporomandibular joint (TMJ) and provide an update of a previous review published in 2010 by summarizing the last 15 years of literature on the topic.</p><p><strong>Methods: </strong>On 07 July 2024, two of the authors independently performed a systematic search in the most relevant medical databases to identify all the English-language papers reporting cases of TMJ SC. After selecting the articles, the authors discussed their potential inclusion in the systematic review, and in case of disagreement, a third author was consulted.</p><p><strong>Results: </strong>From the assessment of all four databases, 466 citations were retrieved. After the removal of the duplicates, 136 articles remained for evaluation. In the end, a total of 108 articles were selected for inclusion, reporting a total of 1046 cases of SC of the TMJ. The most frequently reported signs and symptoms were pain, reduced interincisal mouth opening, swelling, crepitus, and clicking.</p><p><strong>Conclusions: </strong>Despite the different surgical treatment options that were adopted, the recurrence rate remains low. From the present review, no evidence arises for a specific risk factor for TMJ SC. THE PROSPERO REGISTRATION: register number CRD42024568102.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"70-84"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between parafunctional behaviors, clinical diagnoses, psychosocial factors and pain widespreadness in Finnish TMD pain patients in tertiary care.","authors":"Arvid Iljin, Ilona Assila, Ritva Näpänkangas, Tuija Teerijoki-Oksa, Mimmi Tolvanen, Kirsi Sipilä","doi":"10.22514/jofph.2025.074","DOIUrl":"10.22514/jofph.2025.074","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the association of oral parafunctions with clinical DC/TMD (Diagnostic Criteria for Temporomandibular Disorders) Axis I diagnoses, Axis II biopsychosocial assessment and pain widespreadness in TMD pain patients in tertiary care.</p><p><strong>Methods: </strong>197 TMD pain patients were clinically examined and responded to DC/TMD OBC (Oral Behaviour Checklist) and Axis II comprehensive instruments. Patients were divided into Pain Drawing (PD) profile subgroups: PD-1 = head/face pain; PD-2 = head and neck/shoulder regional pain; PD-3 = widespread pain. Using the Graded Chronic Pain Scale 2.0 assessing pain-related intensity/interference, the patients were classified into TMD subtypes 1-3. Associations of frequent sleep bruxism (4-7 times per week) and daytime clenching (most/all of the time) with explanatory variables were evaluated with Independent Samples Kruskal-Wallis and chi-square tests and pairwise comparisons were made with Mann-Whitney U-test with Bonferroni correction.</p><p><strong>Results: </strong>Frequent sleep bruxism was reported by 46.2% and daytime clenching by 67.5% of the participants. Sleep bruxism and daytime clenching associated significantly with muscle-related TMD diagnoses. Sleep bruxism and daytime clenching were significantly associated with anxiety (GAD-7, General Anxiety Disorder-7) subgroups, the highest prevalence being in the most severe subgroups. Frequent sleep bruxism was reported more by participants in TMD subtype 2 as well as those in PD-2 and PD-3 profile subgroups, with significant differences between PD-1 <i>vs.</i> PD-2 and between PD-1 <i>vs.</i> PD-3.</p><p><strong>Conclusions: </strong>Oral parafunctions are associated with muscle-related TMD diagnoses, anxiety symptoms and wider body pain, which should be considered in the assessment, treatment planning and personalized care of TMD pain patients.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"173-183"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research trends in MRI of temporomandibular disorders: a bibliometric study and visualization analysis via CiteSpace.","authors":"Yu Luo, Mengqi Liu, Yujiao Jiang, Kangkang Ma, Zhiye Chen","doi":"10.22514/jofph.2025.072","DOIUrl":"10.22514/jofph.2025.072","url":null,"abstract":"<p><strong>Background: </strong>To conduct a bibliometric analysis mapping the intellectual landscape and emerging trends in Magnetic resonance imaging (MRI) research of temporomandibular disorders (TMD), identifying knowledge gaps and future directions.</p><p><strong>Methods: </strong>A total of 1017 articles were retrieved from the Web of Science Core Collection (WOSCC) database from 1995 to 2024 using the search formula: TS (Topic Search) = (\"Temporomandibular Disorders\" OR \"Temporomandibular Joint Disease\" OR TMD) AND TS = (\"Magnetic Resonance Imaging\" OR MRI). Author/country collaboration network, co-citation analysis, keyword clustering, and burst detection were conducted via CiteSpace 6.4.R1 (Parameters: Time slice: 1 year; g-index <i>k</i> = 25; Log-Likelihood Ratio clustering).</p><p><strong>Results: </strong>Annual publications exhibited triphasic growth, peaking at 75 articles in 2022. The United States (160 articles, centrality = 0.22) dominated global collaborations, while Shanghai Jiao Tong University emerged as the most productive institution (30 articles). Key clusters revealed 15 clusters (<i>Q</i> = 0.4235, <i>S</i> = 0.7521), with core clusters including \"juvenile idiopathic arthritis\" and \"deep learning\". Burst strength identified four major research frontier directions: analysis of pathological characteristics of diseases (morphology with a burst strength of 4.49; anterior disc displacement with a burst strength of 3.69); innovation in imaging examination methods (ultrasonography with a burst strength of 3.61; cone-beam computed tomography with a burst strength of 3.51); development of intelligent diagnostic technologies (diagnostic criteria with a burst strength of 12.09; deep learning with a burst strength of 5.81); and support for clinical management applications (management with a burst strength of 4.62).</p><p><strong>Conclusions: </strong>This study delineates an evolving research paradigm integrating Artificial Intelligence (AI)-driven diagnostics with multimodal imaging.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"150-164"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olfactory abnormalities in patients with migraine: a narrative review of a symptom commonly overlooked by neurologists.","authors":"Dong-Xue Ren, Mao-Mei Song, Ying-Jie Gao, Chang-Xin Li, Sui-Yi Xu","doi":"10.22514/jofph.2025.065","DOIUrl":"10.22514/jofph.2025.065","url":null,"abstract":"<p><p>Nausea, vomiting, photophobia, and phonophobia are common concomitant symptoms in patients with migraine and provide valuable information for headache specialists during consultations. Clinical observations have identified various olfactory abnormalities in patients with migraine, such as osmophobia, olfactory hallucinations, hyperosmia and hyposmia, which are often overlooked by neurologists. These olfactory abnormalities may interact with the trigeminal vascular system, parasympathetic nervous system, and cortical spreading depression. This review aimed to examine the mechanisms underlying olfactory abnormalities in patients with migraine and the clinical correlations between these abnormalities and migraine. Additionally, olfactory training is highlighted as a promising non-pharmacological treatment for migraine.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"31-37"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimensionality and reliability of the Epworth Sleepiness Scale in dental patients referred for oral appliance therapy.","authors":"Ksenija Rener-Sitar, Mike T John, Dennis P Haley, Anthony J DiAngelis, Michael J Howell, Snigdha S Pusalavidyasagar","doi":"10.22514/jofph.2025.071","DOIUrl":"10.22514/jofph.2025.071","url":null,"abstract":"<p><strong>Background: </strong>There is a limited amount of published research on the dimensionality and reliability of the Epworth Sleepiness Scale (ESS) questionnaire for adult patients referred for oral appliance therapy. This information is crucial for dentists, who often lack objective measures of sleep-disordered breathing (SDB) during titration process of an oral appliance. This study investigated the dimensionality and reliability of ESS scores in adult dental patients with SDB undergoing oral appliance treatment.</p><p><strong>Methods: </strong>In 103 dental patients with SDB referred by a physician (mean age: 52.3 ± 13.0 years; 35% female), the dimensionality of the ESS was investigated using exploratory (EFA) and confirmatory factor analyses (CFA) to determine how many scores are needed to characterize the construct. ESS questionnaires were administered twice before treatment. Internal consistency and test-retest reliability were assessed.</p><p><strong>Results: </strong>Horn's parallel analysis suggested a one-factor model. Extracting one factor and standardizing loadings led to strong loadings for all items, ranging from 0.53 to 0.82. The fit indices indicated a good model fit (Comparative Fit Index = 0.999, Root Mean Square Error of Approximation = 0.020, and Standardized Root Mean Square Residual = 0.064). Cronbach's alpha with 95% confidence interval (CI) was 0.85 (0.82-0.88), indicating strong internal consistency. The intraclass correlation coefficient type 2,1 (95% CI) was 0.86 (0.79-0.90), and the weighted kappa ranged from 0.50 to 0.81.</p><p><strong>Conclusions: </strong>In this patient population, the ESS reliably characterizes excessive daytime sleepiness with a single score and appears suitable for individual assessment in dental patients undergoing oral appliance treatment for SDB.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"138-149"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> effects of dual wavelength photobiomodulation on monocytic response in painful temporomandibular disorder.","authors":"Maria Gabriela Rodriguez, Mathew Warchol, Selenia Rubio, Patricia Cabrera, Rory Reever, Sherif Hosney, Cesar A Migliorati, Frank C Gibson Iii, Richard Ohrbach, Roger B Fillingim, Shannon Wallet, Margarete C Ribeiro Dasilva","doi":"10.22514/jofph.2025.082","DOIUrl":"10.22514/jofph.2025.082","url":null,"abstract":"<p><p>Temporomandibular disorders (TMD) are a prevalent source of chronic pain and disability, yet current therapies often provide limited relief with notable side effects. Photobiomodulation (PBM) has emerged as a promising non-pharmacologic approach to modulate inflammation and pain. This study investigated the effects of a dual-wavelength PBM protocol (laser + light-emitting diode (LED)) on the lipopolysaccharide (LPS)-stimulated monocyte response from individuals with painful TMD. Monocytes were isolated from 16 individuals with TMD enrolled in a randomized, placebo-controlled clinical trial (NCT05916235). Cells were plated into 96-well plates and divided into Control, LPS, and LPS + PBM treatment groups. Monocytes received four alternating-day treatments with two PBM probes: a laser probe (810/660 nm, 180 J/cm²) and an LED probe (660/850 nm). Cytokine and chemokine levels in culture supernatants were measured via multiplexed immunoassays. To verify cell viability after PBM treatment, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used. Statistical analysis was conducted using one-way analysis of variance (ANOVA) with Bonferroni correction, and <i>p</i> < 0.05 was considered significant. PBM significantly reduced levels of pro-inflammatory mediators interleukin-1 beta (IL-1β) (<i>p</i> = 0.005), C-X-C motif chemokine ligand 9 (CXCL9) (<i>p</i> = 0.0042), interferon gamma-induced protein 10 (IP-10) (<i>p</i> = 0.001), and tumor necrosis factor-alpha (TNF-α) (<i>p</i> = 0.0003), while increasing expression of regulatory mediators interleukin-10 (IL-10) (<i>p</i> = 0.0009), interleukin-1 receptor antagonist (IL-1RA) (<i>p</i> = 0.004), and CC motif chemokine ligand 17 (CCL17) (<i>p</i> = 0.003).These findings suggest that the dual-wavelength PBM protocol was able to shift the monocyte immune response from a more pro-inflammatory (M1) phenotype to a more anti-inflammatory, tissue-repair (M2) cytokine profile. These results provide additional evidence for PBM as a benign and non-invasive technique to control and potentially modify TMD-related inflammation. ClinicalTrials.gov ID: NCT05916235.</p>","PeriodicalId":48800,"journal":{"name":"Journal of Oral & Facial Pain and Headache","volume":"39 4","pages":"252-257"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145821615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}