Journal of Periodontal and Implant Science最新文献

{"title":"Metformin enhances the osteogenic activity of rat bone marrow mesenchymal stem cells by inhibiting oxidative stress induced by diabetes mellitus: an <i>in vitro</i> and <i>in vivo</i> study.","authors":"Kai Dong,&nbsp;Wen-Juan Zhou,&nbsp;Zhong-Hao Liu","doi":"10.5051/jpis.2106240312","DOIUrl":"https://doi.org/10.5051/jpis.2106240312","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to determine whether metformin (MF) could alleviate the expresssion of reactive oxygen species (ROS) and improve the osteogenic ability of bone marrow mesenchymal stem cells derived from diabetic rats (drBMSCs) <i>in vitro</i>, and to evaluate the effect of MF on the ectopic osteogenesis of drBMSCs in a nude mouse model <i>in vivo</i>.</p><p><strong>Methods: </strong>BMSCs were extracted from normal and diabetic rats. <i>In vitro</i>, a cell viability assay (Cell Counting Kit-8), tests of alkaline phosphatase (ALP) activity, and western blot analysis were first used to determine the cell proliferation and osteogenic differentiation of drBMSCs that were subjected to treatment with different concentrations of MF (0, 50, 100, 200, 500 μM). The cells were then divided into 5 groups: (1) normal rat BMSCs (the BMSCs derived from normal rats group), (2) the drBMSCs group, (3) the drBMSCs + Mito-TEMPO (10 μM, ROS scavenger) group, (4) the drBMSCs + MF (200 μM) group, and (5) the drBMSCs + MF (200 μM) + H₂O₂ (50 μM, ROS activator) group. Intracellular ROS detection, a senescence-associated β-galactosidase assay, ALP staining, alizarin red staining, western blotting, and immunofluorescence assays were performed to determine the effects of MF on oxidative stress and osteogenic differentiation in drBMSCs. <i>In vivo</i>, the effect of MF on the ectopic osteogenesis of drBMSCs was evaluated in a nude mouse model.</p><p><strong>Results: </strong>MF effectively reduced ROS levels in drBMSCs. The cell proliferation, ALP activity, mineral deposition, and osteogenic-related protein expression of drBMSCs were demonstrably higher in the MF-treated group than in the non-MF-treated group. H₂O₂ inhibited the effects of MF. In addition, ectopic osteogenesis was significantly increased in drBMSCs treated with MF.</p><p><strong>Conclusions: </strong>MF promoted the proliferation and osteogenic differentiation of drBMSCs by inhibiting the oxidative stress induced by diabetes and enhenced the ectopic bone formation of drBMSCs in nude mice.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/74/e2/jpis-53-54.PMC9943706.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10750476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial profiling of peri-implantitis compared to the periodontal microbiota in health and disease using 16S rRNA sequencing.","authors":"Hyun-Joo Kim,&nbsp;Dae-Hee Ahn,&nbsp;Yeuni Yu,&nbsp;Hyejung Han,&nbsp;Si Yeong Kim,&nbsp;Ji-Young Joo,&nbsp;Jin Chung,&nbsp;Hee Sam Na,&nbsp;Ju-Youn Lee","doi":"10.5051/jpis.2202080104","DOIUrl":"https://doi.org/10.5051/jpis.2202080104","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of this study was to analyze the microbial profile of individuals with peri-implantitis (PI) compared to those of periodontally healthy (PH) subjects and periodontitis (PT) subjects using Illumina sequencing.</p><p><strong>Methods: </strong>Buccal, supragingival, and subgingival plaque samples were collected from 109 subjects (PH: 30, PT: 49, and PI: 30). The V3-V4 region of 16S rRNA was sequenced and analyzed to profile the plaque microbiota.</p><p><strong>Results: </strong>Microbial community diversity in the PI group was higher than in the other groups, and the 3 groups showed significantly separated clusters in the buccal samples. The PI group showed different patterns of relative abundance from those in the PH and PT groups depending on the sampling site at both genus and phylum levels. In all samples, some bacterial species presented considerably higher relative abundances in the PI group than in the PH and PT groups, including <i>Anaerotignum lactatifermentans</i>, <i>Bacteroides vulgatus</i>, <i>Faecalibacterium prausnitzii</i>, <i>Olsenella uli</i>, <i>Parasutterella excrementihominis</i>, <i>Prevotella buccae</i>, <i>Pseudoramibacter alactolyticus</i>, <i>Treponema parvum</i>, and <i>Slackia exigua</i>. Network analysis identified that several well-known periodontal pathogens and newly recognized bacteria were closely correlated with each other.</p><p><strong>Conclusions: </strong>The composition of the microbiota was considerably different in PI subjects compared to PH and PT subjects, and these results could shed light on the mechanisms involved in the development of PI.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/94/e6/jpis-53-69.PMC9943702.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential anti-inflammatory and osteogenic effects of a dual drug delivery scaffold loaded with parthenolide and naringin in periodontitis.","authors":"Rui Chen,&nbsp;Mengting Wang,&nbsp;Qiaoling Qi,&nbsp;Yanli Tang,&nbsp;Zhenzhao Guo,&nbsp;Shuai Wu,&nbsp;Qiyan Li","doi":"10.5051/jpis.2105700285","DOIUrl":"https://doi.org/10.5051/jpis.2105700285","url":null,"abstract":"<p><strong>Purpose: </strong>Our pilot study showed that a 3-dimensional dual drug delivery scaffold (DDDS) loaded with Chinese herbs significantly increased the regenerated bone volume fraction. This study aimed to confirm the synergistic anti-inflammatory and osteogenic preclinical effects of this system.</p><p><strong>Methods: </strong>The targets and pathways of parthenolide and naringin were predicted. Three cell models were used to assess the anti-inflammatory effects of parthenolide and the osteogenic effects of naringin. First, the distance between the cementoenamel junction and alveolar bone crest (CEJ-ABC) and the bone mineral density (BMD) of surgical defects were measured in a rat model of periodontitis with periodontal fenestration defects. Additionally, the mRNA expression levels of matrix metallopeptidase 9 (MMP9) and alkaline phosphatase (ALP) were measured. Furthermore, the number of inflammatory cells and osteoclasts, as well as the protein expression levels of tumor necrosis factor-alpha (TNF-α) and levels of ALP were determined.</p><p><strong>Results: </strong>Target prediction suggested prostaglandin peroxidase synthase (PTGS2) as a potential target of parthenolide, while cytochrome P450 family 19 subfamily A1 (CYP19A1) and taste 2 receptor member 31 (TAS2R31) were potential targets of naringin. Parthenolide mainly targeted inflammation-related pathways, while naringin participated in steroid hormone synthesis and taste transduction. <i>In vitro</i> experiments revealed significant anti-inflammatory effects of parthenolide on RAW264.7 cells, and significant osteogenic effects of naringin on bone marrow mesenchymal stem cells and MC3T3-E1 cells. DDDS loaded with parthenolide and naringin decreased the CEJ-ABC distance and increased BMD and ALP levels in a time-dependent manner. Inflammation was significantly alleviated after 14 days of DDDS treatment. Additionally, after 56 days, the DDDS group exhibited the highest BMD and ALP levels.</p><p><strong>Conclusions: </strong>DDDS loaded with parthenolide and naringin in a rat model achieved significant synergistic anti-inflammatory and osteogenic effects, providing powerful preclinical evidence.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/01/c5/jpis-53-20.PMC9943701.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the stage and grade of periodontitis according to the current classification of periodontal and peri-implant diseases and conditions (2018) using machine learning algorithms.","authors":"Kübra Ertaş, Ihsan Pence, Melike Siseci Cesmeli, Zuhal Yetkin Ay","doi":"10.5051/jpis.2201060053","DOIUrl":"10.5051/jpis.2201060053","url":null,"abstract":"<p><strong>Purpose: </strong>The current Classification of Periodontal and Peri-Implant Diseases and Conditions, published and disseminated in 2018, involves some difficulties and causes diagnostic conflicts due to its criteria, especially for inexperienced clinicians. The aim of this study was to design a decision system based on machine learning algorithms by using clinical measurements and radiographic images in order to determine and facilitate the staging and grading of periodontitis.</p><p><strong>Methods: </strong>In the first part of this study, machine learning models were created using the Python programming language based on clinical data from 144 individuals who presented to the Department of Periodontology, Faculty of Dentistry, Süleyman Demirel University. In the second part, panoramic radiographic images were processed and classification was carried out with deep learning algorithms.</p><p><strong>Results: </strong>Using clinical data, the accuracy of staging with the tree algorithm reached 97.2%, while the random forest and k-nearest neighbor algorithms reached 98.6% accuracy. The best staging accuracy for processing panoramic radiographic images was provided by a hybrid network model algorithm combining the proposed ResNet50 architecture and the support vector machine algorithm. For this, the images were preprocessed, and high success was obtained, with a classification accuracy of 88.2% for staging. However, in general, it was observed that the radiographic images provided a low level of success, in terms of accuracy, for modeling the grading of periodontitis.</p><p><strong>Conclusions: </strong>The machine learning-based decision system presented herein can facilitate periodontal diagnoses despite its current limitations. Further studies are planned to optimize the algorithm and improve the results.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/1d/jpis-53-38.PMC9943704.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10750477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a simplified drilling protocol at 50 rpm on heat generation under water-free conditions: an <i>in vitro</i> study.","authors":"Hyeon-Ji Jang,&nbsp;Jin-Un Yoon,&nbsp;Ji-Young Joo,&nbsp;Ju-Youn Lee,&nbsp;Hyun-Joo Kim","doi":"10.5051/jpis.2201400070","DOIUrl":"https://doi.org/10.5051/jpis.2201400070","url":null,"abstract":"<p><strong>Purpose: </strong>In recent years, guided implant surgery has been widely used for the convenience of patients and surgeons. Further streamlining the surgical procedure would make implant surgery more convenient. Low-speed water-free conditions are often used in guided implant surgery. Therefore, in this study, we attempted to confirm once again whether drilling was safe at a low speed without water. The main purpose of this study was to evaluate whether a simplified drilling protocol that omits some intermediate steps in the drilling process was safe from the viewpoint of heat generation.</p><p><strong>Methods: </strong>D1 density artificial bone blocks were drilled under 50 rpm, 10 N·cm water-free conditions, and the surface temperature was measured using a digital infrared camera. First, drilling was performed with the sequential drilling method, which is the most widely used technique. Second, for each drill diameter, the temperature change was measured while performing simplified drilling with omission of the previous 1, 2, or 3 steps.</p><p><strong>Results: </strong>In sequential drilling, the heat generated during drilling at all diameters was less than the critical temperature of osteonecrosis (47°C) except for the ⌀2 drill. Statistical significance was observed in all groups when comparing sequential and simplified drilling in the ⌀3.2, ⌀3.8, and ⌀4.3 drills (<i>P</i><0.001). However, in the simplified drilling procedures, the temperature was below the osteonecrosis threshold temperature (47°C) except for the ⌀4.3 drill with the omission of the previous 3 steps (⌀3.0, ⌀3.2, and ⌀3.8).</p><p><strong>Conclusions: </strong>In general, drilling under low-speed, water-free conditions has shown stable results in terms of heat generation. Simplified drilling showed statistically significantly greater heat generation than sequential drilling. However, most of the diameters and omitted steps seem to be clinically acceptable, so it will be useful if an appropriate selection is made according to the patient's clinical condition.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/d7/jpis-53-85.PMC9943703.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do platelet concentrates accelerate orthodontic tooth movement?: a systematic review.","authors":"Sergio Herrero-Llorente,&nbsp;Angel-Orión Salgado-Peralvo,&nbsp;Jan G J H Schols","doi":"10.5051/jpis.2201600080","DOIUrl":"https://doi.org/10.5051/jpis.2201600080","url":null,"abstract":"<p><strong>Purpose: </strong>Surgical techniques in orthodontics have received widespread attention in recent years. Meanwhile, biomaterials with high molecular content have been introduced, such as platelet concentrates (PCs), which may accelerate orthodontic tooth movement (OTM) and reduce periodontal damage. The present systematic review aimed to answer the following PICO question: \"In patients in whom orthodontic surgical techniques are performed (P), what is the effectiveness of using PCs over the surgical site (I) when compared to not placing PCs (C) to achieve faster tooth movement (O)?\"</p><p><strong>Methods: </strong>A search was performed in 6 databases. The criteria employed were those described in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses declaration. The present review included studies with a control group that provided information about the influence of PCs on the rate of OTM.</p><p><strong>Results: </strong>The electronic search identified 10 studies that met the established criteria.</p><p><strong>Conclusions: </strong>The included studies were very diverse, making it difficult to draw convincing conclusions. However, a tendency was observed for OTM to be accelerated when PCs were used as an adjuvant for canine distalization after premolar extraction when distalization was started in the same session. Likewise, studies seem to indicate an association between PC injection and the amount of canine retraction. However, it is not possible to affirm that the use of PCs in corticotomy shortens the overall treatment time, as this question has not been studied adequately.</p><p><strong>Trial registration: </strong>PROSPERO Identifier: CRD42021278542.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/b3/jpis-53-2.PMC9943705.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term effect of implant-abutment connection type on marginal bone loss and survival of dental implants.","authors":"Young-Min Kim,&nbsp;Jong-Bin Lee,&nbsp;Heung-Sik Um,&nbsp;Beom-Seok Chang,&nbsp;Jae-Kwan Lee","doi":"10.5051/jpis.2200960048","DOIUrl":"https://doi.org/10.5051/jpis.2200960048","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to compare the long-term survival rate and peri-implant marginal bone loss between different types of dental implant-abutment connections.</p><p><strong>Methods: </strong>Implants with external or internal abutment connections, which were fitted at Gangneung-Wonju National University Dental Hospital from November 2011 to December 2015 and followed up for >5 years, were retrospectively investigated. Cumulative survival rates were evaluated for >5 years, and peri-implant marginal bone loss was evaluated at 1- and 5-year follow-up examinations after functional loading.</p><p><strong>Results: </strong>The 8-year cumulative survival rates were 93.3% and 90.7% in the external and internal connection types, respectively (<i>P</i>=0.353). The mean values of marginal bone loss were 1.23 mm (external) and 0.72 mm (internal) (<i>P</i><0.001) after 1 year of loading, and 1.20 mm and 1.00 mm for external and internal abutment connections, respectively (<i>P=</i>0.137) after 5 years. Implant length (longer, <i>P</i>=0.018), smoking status (heavy, <i>P</i>=0.001), and prosthetic type (bridge, <i>P</i>=0.004) were associated with significantly greater marginal bone loss, and the use of screw-cement-retained prosthesis was significantly associated (<i>P</i>=0.027) with less marginal bone loss.</p><p><strong>Conclusions: </strong>There was no significant difference in the cumulative survival rate between implants with external and internal abutment connections. After 1 year of loading, marginal bone loss was greater around the implants with an external abutment connection. However, no significant difference between the external and internal connection groups was found after 5 years. Both types of abutment connections are viable treatment options for the reconstruction of partially edentulous ridges.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/de/jpis-52-496.PMC9807847.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10854493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and patient-reported outcomes after recession coverage using modified vestibular incision subperiosteal tunnel access with a volume-stable collagen matrix as compared to a coronally advanced flap with a subepithelial connective tissue graft.","authors":"Chun-Teh Lee,&nbsp;Marlena Lange,&nbsp;Alain Jureidini,&nbsp;Nurit Bittner,&nbsp;Ulrike Schulze-Späte","doi":"10.5051/jpis.2105760288","DOIUrl":"https://doi.org/10.5051/jpis.2105760288","url":null,"abstract":"<p><strong>Purpose: </strong>Coronally advanced split-or full-thickness (CAST or CAFT) flaps in combination with subepithelial connective tissue grafts (SCTGs) are commonly used in root-coverage procedures despite postoperative pain and bleeding from the graft donor site. Therefore, the modified vestibular incision subperiosteal tunnel access procedure (VISTAX) uses a novel collagen matrix (VCMX) instead of autogenous tissue to address the limitations associated with autogenous tissue grafting. This retrospective study compared the clinical outcomes of VISTAX to the results obtained after using a CAST or CAFT flap in combination with SCTG for root coverage.</p><p><strong>Methods: </strong>Patients with single or multiple adjacent recession I/II defects were included, with 10 subjects each in the VISTAX, CAFT, and CAST groups. Defect coverage, keratinized tissue width, esthetic scores, and patients' perceived pain and dentinal hypersensitivity (visual analogue scale [VAS]) were assessed at baseline, 3 months, and 6 months.</p><p><strong>Results: </strong>All surgical techniques significantly reduced gingival recession (<i>P</i><0.0001). Defect coverage, esthetic appearance, and the reduction in dentinal hypersensitivity were comparable. However, the VAS scores for pain were significantly lower in the VISTAX group than in the CAFT and CAST groups, which had similar scores (<i>P</i><0.05). Furthermore, the clinical results of VISTAX and CAFT/CAST generally remained stable at 6 months.</p><p><strong>Conclusions: </strong>The clinical outcomes of VISTAX, CAFT, and CAST were comparable. However, patients perceived significantly less pain after VISTAX, indicating a potentially higher patient acceptance of the procedure. A prospective trial with a longer follow-up period and a larger sample size should therefore evaluate VISTAX further.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/ab/jpis-52-466.PMC9807852.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10854491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rodent peri-implantitis models: a systematic review and meta-analysis of morphological changes.","authors":"Ren Jie Jacob Chew,&nbsp;Jacinta Xiaotong Lu,&nbsp;Yu Fan Sim,&nbsp;Alvin Boon Keng Yeo","doi":"10.5051/jpis.2200900045","DOIUrl":"https://doi.org/10.5051/jpis.2200900045","url":null,"abstract":"<p><strong>Purpose: </strong>Rodent models have emerged as an alternative to established larger animal models for peri-implantitis research. However, the construct validity of rodent models is controversial due to a lack of consensus regarding their histological, morphological, and biochemical characteristics. This systematic review sought to validate rodent models by characterizing their morphological changes, particularly marginal bone loss (MBL), a hallmark of peri-implantitis.</p><p><strong>Methods: </strong>This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A literature search was performed electronically using MEDLINE (PubMed), and Embase, identifying pre-clinical studies reporting MBL after experimental peri-implantitis induction in rodents. Each study's risk of bias was assessed using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk of bias tool. A meta-analysis was performed for the difference in MBL, comparing healthy implants to those with experimental peri-implantitis.</p><p><strong>Results: </strong>Of the 1,014 unique records retrieved, 23 studies that met the eligibility criteria were included. Peri-implantitis was induced using 4 methods: ligatures, lipopolysaccharide, microbial infection, and titanium particles. Studies presented high to unclear risks of bias. During the osseointegration phase, 11.6% and 6.4%-11.3% of implants inserted in mice and rats, respectively, had failed to osseointegrate. Twelve studies were included in the meta-analysis of the linear MBL measured using micro-computed tomography. Following experimental peri-implantitis, the MBL was estimated to be 0.25 mm (95% confidence interval [CI], 0.14-0.36 mm) in mice and 0.26 mm (95% CI, 0.19-0.34 mm) in rats. The resulting peri-implant MBL was circumferential, consisting of supra- and infrabony components.</p><p><strong>Conclusions: </strong>Experimental peri-implantitis in rodent models results in circumferential MBL, with morphology consistent with the clinical presentation of peri-implantitis. While rodent models are promising, there is still a need to further characterize their healing potentials, standardize experiment protocols, and improve the reporting of results and methodology.</p><p><strong>Trial registration: </strong>PROSPERO Identifier: CRD42020209776.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/ae/jpis-52-479.PMC9807853.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10854492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival crevicular fluid CSF-1 and IL-34 levels in patients with stage III grade C periodontitis and uncontrolled type 2 diabetes mellitus.","authors":"Ahu Dikilitaş, Fatih Karaaslan, Şehrazat Evirgen, Abdullah Seçkin Ertuğrul","doi":"10.5051/jpis.2106260313","DOIUrl":"10.5051/jpis.2106260313","url":null,"abstract":"<p><strong>Purpose: </strong>Periodontal diseases are inflammatory conditions that alter the host's response to microbial pathogens. Type 2 diabetes mellitus (T2DM) is a complex disease that affects the incidence and severity of periodontal diseases. This study investigated the gingival crevicular fluid (GCF) levels of colony-stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) in patients with stage III grade C periodontitis (SIII-GC-P) and stage III grade C periodontitis with uncontrolled type 2 diabetes (SIII-GC-PD).</p><p><strong>Methods: </strong>In total, 72 individuals, including 24 periodontally healthy (PH), 24 SIII-GC-P, and 24 SIII-GC-PD patients, were recruited for this study. Periodontitis patients (stage III) had interdental attachment loss (AL) of 5 mm or more, probing depth (PD) of 6 mm or more, radiographic bone loss advancing to the middle or apical part of the root, and tooth loss (<5) due to periodontal disease. Radiographic bone loss in the teeth was also evaluated; grade C periodontitis was defined as a ratio of the percentage of root bone loss to age greater than 1.0. The plaque index (PI), gingival index (GI), presence of bleeding on probing (BOP), PD, and clinical AL were used for clinical periodontal assessments. GCF samples were obtained and analyzed using an enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>All clinical parameters-PD, AL, GI, BOP, and PI-were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups for both the full mouth and each sampling site (<i>P</i><0.05). The total IL-34 and CSF-1 levels were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups (<i>P</i><0.05), and there were significant differences between the periodontitis groups (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>These findings suggest that IL-34 and CSF-1 expression increases in patients with SIII-GC-PD. CSF-1 was associated with the inflammatory status of periodontal tissues and T2DM, while IL-34 was associated only with T2DM.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov Identifier: NCT04891627.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/ec/jpis-52-455.PMC9807851.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10838074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}