Arab Journal of Gastroenterology最新文献

{"title":"Washed microbiota transplantation combined with biological agents promotes histological remission in refractory severe ulcerative colitis with recurrent intestinal infection: A case report.","authors":"Qiong Wu, Liu-Si Yang, Hong-Li Huang, Ying-Fei Li, Yong-Jian Zhou, Hao-Ming Xu","doi":"10.1016/j.ajg.2024.07.008","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.07.008","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a chronic non-specific colitis disease. In recent years, fecal microbiota transplantation (FMT), including improved washed microbiota transplantation (WMT), and biological agents have helped improve the prognosis of patients with UC. However, a significant number of patients with moderate to severe UC do not get relief from glucocorticoids, immunosuppressants, and TNF-α antagonists. Patients with severe UC are frequently burdened with opportunistic infections and subsequent surgical interventions. Combined treatment modalities are crucial for patients with severe UC and opportunistic infections. Herein, we reported a case of a 25-year-old female with refractory severe UC complicated with recurrent Clostridioides difficile infection and recurrent cytomegalovirus infection for six years. Surgical removal of the affected bowel segment was almost unavoidable. She showed endoscopic and histological recovery after comprehensive WMT and Vedolizumab treatment. The following are our learnings from the case: 1. A combination of WMT and biological agents can potentially obviate the necessity for surgical treatment in patients with refractory severe UC and promote histological remission. 2. Personalized comprehensive treatment and chronic disease management models for patients with UC should be emphasized. 3. WMT can help treat opportunistic infections, which may also strengthen the treatment with gut-targeted biological agents when traditional TNF-α antagonists show poor efficacy.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of diagnosis model for inflammatory bowel diseases based on a serologic biomarker panel: A decision tree model study.","authors":"Junxiang Zeng, Manxiu Huai, Wensong Ge, Zhigang Yang, Xiujun Pan","doi":"10.1016/j.ajg.2024.05.003","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.05.003","url":null,"abstract":"<p><strong>Background and study aims: </strong>Currently, an increasing amount of experimental data is available on newly discovered biomarkers in inflammatory bowel diseases (IBD), but the role of these biomarkers is often questionable due to their limited sensitivity. Therefore, this study aimed to build a diagnostic tool incorporating a panel of serum biomarkers into a computational algorithm to identify patients with IBD and differentiate those with Crohn's disease (CD) from those with ulcerative colitis (UC).</p><p><strong>Patients and methods: </strong>We studied sera from 192 CD patients, 118 UC patients, 60 non-IBD controls and 60 healthy controls. Indirect immunofluorescence (IIF) assays were utilized to determine several serum biomarkers previously associated with IBD, and the decision tree algorithm was used to construct the diagnosis model. Performances of models were evaluated by prediction accuracy, precision, AUC and Matthews's correlation coefficient (MCC). The \"Inflammatory Bowel Disease Multi-omics Database (IBDMDB)\" cohorts were used to validate the model as external validation set.</p><p><strong>Results: </strong>The prediction rates were determined and compared for decision tree models after each data was developed using C5.0, C&RT, QUEST and CHAID. The C5.0 and CHAID algorithms, which ranked top for the prediction rate in the IBD vs. non-IBD model and the CD vs. UC model, respectively, were utilized for final pattern analysis. The final decision tree model achieved higher classification accuracy than the approach based on conservative marker combinations (sensitivity 75.0% vs. 79.5%, specificity 93.8% vs. 78.3% for differentiating IBD from non-IBD; and sensitivity 84.3% vs. 73.4%, specificity 92.5% vs. 54.9% for differentiating CD from UC, respectively). The model prediction consistency was 93% (28/30) in the external validation set.</p><p><strong>Conclusion: </strong>The decision-tree-based approach used in this study, based on serum biomarkers, has shown to be a valid and useful approach to identifying IBD and differentiating CD from UC.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoscopic characterization of gastrointestinal manifestations in children with undifferentiated recurrent fever.","authors":"Nilüfer Ülkü Şahin, Nihal Şahin","doi":"10.1016/j.ajg.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.07.004","url":null,"abstract":"<p><strong>Background and study aims: </strong>Systemic autoinflammatory diseases are characterized by recurrent or chronic inflammation, and monogenic forms are increasingly defined. However, a group of patients without genetic diagnosis is called the syndrome of undifferentiated recurrent fever (SURF). This study analyzed the clinical and endoscopic features of patients with SURF presenting with gastrointestinal (GI) symptoms.</p><p><strong>Patients and methods: </strong>Between 2019 and 2022, GI endoscopy were performed in patients with SURF who presented with GI symptoms. Clinical, genetic, laboratory, and endoscopy findings were analyzed.</p><p><strong>Results: </strong>Fifteen patients were included in the study, eight (53.3 %) were girls. The mean age was 10.5 ± 5.80 years, and the median age at symptom onset was 4 (0.3-16) years. All patients experienced fever and abdominal pain. Thirteen patients (86.7 %) experienced diarrhea, 11 (73.3 %) reported myalgia, and 10 (66.7 %) had joint involvement. Lymphoid follicles in the terminal ileum mucosa were detected in 10 patients (66.7 %), and nodular lymphoid hyperplasia in the terminal ileum was the histopathological finding in 12 patients (80 %).</p><p><strong>Conclusions: </strong>The current study found that patients with SURF experiencing gastrointestinal symptoms have excessive lymph node formation in the terminal ileal mucosa due to an exaggerated inflammatory response. This may be the cause of their GI symptoms.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The silent epidemic: Inappropriate use of proton pump inhibitors among hospitalized patients.","authors":"Mohamed Ramadan Mohamed, Mira Itani, Mostafa Abohelwa, Mohamed Attia Ahmed, Lina Abdouni, George Doumat, Majduldeen Azzo, Fatema Dabdoub, Hanana Al-Tfaili, Moustafa Elziny, Georges Assaf","doi":"10.1016/j.ajg.2024.07.001","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.07.001","url":null,"abstract":"<p><strong>Background and study aims: </strong>There is an increasing trend to inappropriately prescribe proton pump inhibitors (PPIs) in different clinical settings despite the reported adverse outcomes. This study aimed to assess (1) the prevalence of potentially inappropriate use of PPIs and its associated risk factors among hospitalized patients, at pre-admission and discharge and (2) the prevalence of valid indications of PPIs use without prescription.</p><p><strong>Patients and methods: </strong>A retrospective observational study was performed at a single center, examining the records of patients aged ≥18 years who were admitted to the Family Medicine inpatient service over a one-year period. The appropriateness of PPIs use was assessed against a set of pre-approved indications.</p><p><strong>Results: </strong>A total of 289 patients were included in the analysis. Of these, 34.67 % were taking PPIs upon admission, increasing to 43.67 % at discharge (p < 0.001). Inappropriate PPI use was identified in 51.92 % at pre-admission and 57.25 % at discharge. Multivariate analysis identified significant factors contributing to inappropriate PPI use: polypharmacy at both admission and discharge (OR = 4.587, p = 0.031), and the presence of two or more comorbidities at discharge (OR = 5.421, p = 0.011; OR = 13.005, p = 0.037). Age ≤65 was associated with increased inappropriate use only at discharge (p < 0.003). Conversely, appropriate prescribing was noted in patients over 65 and those on antiplatelet therapy, aligning with clinical guidelines.</p><p><strong>Conclusions: </strong>This study reveals a high prevalence of inappropriate PPI use among hospitalized patients, notably increasing from admission to discharge. Key contributors to inappropriate PPI usage included polypharmacy and high comorbidity scores at discharge, particularly in patients under 65. This emphasizes the need for targeted interventions to optimize PPI prescribing practices in clinical settings.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adherent-invasive Escherichia coli LF82 disrupts the tight junctions of Caco-2 monolayers.","authors":"Sumei Sha, Huijun Gao, Hong Zeng, Fenrong Chen, Junxiu Kang, Yan Jing, Xin Liu, Bin Xu","doi":"10.1016/j.ajg.2024.07.011","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.07.011","url":null,"abstract":"<p><strong>Background and study aims: </strong>Adherent invasive Escherichia coli (AIEC) are enriched in IBD (inflammatory bowel disease) patients, but the role and mechanism of AIEC in the intestinal epithelial barrier is poorly defined. We evaluated the role of the AIEC strain E. coli LF82 in vitro and investigated the role of Th17 in this process.</p><p><strong>Material and methods: </strong>After coincubation with AIEC, the epithelial barrier integrity was monitored by epithelial resistance measurements. The permeability of the barrier was evaluated by TEER (trans-epithelial electrical resistance) and mucosal-to-serosal flux rate. The presence of interepithelial tight junction proteins ZO-1 and Claudin-1 were determined by immunofluorescence and western blot analysis. Cytokines in the cell culture supernatant were assayed by enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>AIEC infection decreased TEER and increased the mucosal-to-serosal flux rate of Lucifer yellow in the intestinal barrier model in a time- and dose-dependent manner. AIEC infection decreased the expression and changed the distribution of ZO-1 and claudin-1. It also induced the secretion of cytokines such as TNF-α and IL-17.</p><p><strong>Conclusion: </strong>AIEC strain E. coli LF82 increased the permeability and disrupted the tight junctions of the intestinal epithelial barrier, revealing that AIEC plays an aggravative role in the inflammatory response.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Education level and biologic therapy are the related factors of mucosal healing in Patients with Crohn's disease.","authors":"Limin Zhang, Shanbing Yang, Kangmei Jia, Shuwen Du, Yan Jia, Xiaojuan Lu, Jiheng Wang","doi":"10.1016/j.ajg.2024.06.003","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.06.003","url":null,"abstract":"<p><strong>Background and study aims: </strong>Mucosal healing (MH) is a crucial indicator of therapeutic effectiveness and prognosis in Crohn's disease (CD). Rapid achievement and long-term maintenance of MH can alleviate the financial and psychological burden on patients. This study aimed to investigate the factors associated with MH in CD patients and enhance clinicians' understanding.</p><p><strong>Patients and methods: </strong>Patients diagnosed with CD between January 2010 and December 2019 at our hospital were included and divided into two groups based on the attainment of MH during the follow-up period. Demographic data, symptoms, disease classification, laboratory examination results, and treatments were collected and compared between the two groups. Factors with a P-value <0.2 were subjected to multivariate logistic regression analysis to identify the related factors of MH.</p><p><strong>Results: </strong>Multivariate logistic regression analysis of CD patients revealed that educational level [odds ratio (OR) = 8.167, 95 % confidence interval (CI) 1.440-46.303, P = 0.018] and biological therapy (OR = 15.291, 95 % CI 1.404-166.543, P = 0.025) were associated with MH.</p><p><strong>Conclusion: </strong>Educational level and biological therapy are factors related to MH in CD patients. These findings suggest that the use of biological therapy and patients' better understanding of the disease contribute to achieving MH.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term effect of gluten-free diet on disease severity, quality of life, and inflammatory markers among patients with mild to moderate ulcerative colitis: A triple-blind randomized placebo-controlled trial.","authors":"Foroogh Alborzi Avanaki, Naser Ebrahimi Daryani, Najmeh Aletaha, Nazanin Hesabgar, Mohammad Saeid Rezaee-Zavareh, Reza Hadi","doi":"10.1016/j.ajg.2024.01.009","DOIUrl":"https://doi.org/10.1016/j.ajg.2024.01.009","url":null,"abstract":"<p><strong>Background and study aims: </strong>Diet is an important underlying factor in ulcerative colitis (UC) disease. The present study aimed to investigate the effect of a gluten-free diet (GFD) on disease severity, quality of life, and inflammatory markers in patients with UC.</p><p><strong>Patients and methods: </strong>In this triple-blind randomized placebo-controlled clinical trial, we evaluated the effect of a GFD on the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), fecal calprotectin, disease severity, and quality of life in patients with mild to moderate UC. Patients' quality of life and severity of symptoms were evaluated using the Inflammatory Bowel Disease Questionnaire (IBDQ) and Simple Clinical Colitis Activity Index (SCCAI), respectively. Patients received this regimen for six weeks and were evaluated before and after the intervention.</p><p><strong>Results: </strong>The mean age of patients (n = 26) was 39.31 years (standard deviation = 9.34). In both study groups, the mean ESR, CRP, IBDQ, and SCCAI showed no statistically significant improvement with the dietary intervention. Fecal calprotectin was increased in both groups without statistical significance.</p><p><strong>Conclusions: </strong>We could not find any significant effect of GFD on inflammatory markers, quality of life, and disease severity among patients with mild to moderate UC. It is too early to suggest the gluten-free diet as a safe and beneficial regimen for UC patients. There is a need for further investigations with larger sample sizes and longer follow-ups as clinical trials and cohort studies to obtain more reliable results.</p>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140877691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonographic features of Lemmel’s syndrome: Differential diagnosis from stones in the lower portion of the common bile duct","authors":"Jinxiu Ju ,&nbsp;Yating Su ,&nbsp;Qingjing Zeng ,&nbsp;Jiayi Liang ,&nbsp;Xiaodan Zhang ,&nbsp;Jie Ren ,&nbsp;Lili Wu ,&nbsp;Rongqin Zheng","doi":"10.1016/j.ajg.2024.01.003","DOIUrl":"10.1016/j.ajg.2024.01.003","url":null,"abstract":"<div><h3>Background and study aims</h3><p>Periampullary diverticula (PAD), a location-specific type of duodenal diverticula, can cause serious complications. PAD is difficult to differentially diagnose, mainly due to its nonspecific symptoms. This study aimed to identify sonographic features of PAD and to evaluate their value in the differential diagnosis of PAD from stones in the lower common bile duct (CBD).</p></div><div><h3>Patients and Methods</h3><p>A total of 30 patients with PAD and 60 patients with lower CBD stones were retrospectively enrolled, and sonographic features were analyzed. Measurements of sonographic features included echo shaped, posterior echo changes, location and relation to surrounding organs, and status of intrahepatic and extrahepatic bile duct dilation, and their diagnostic performance was assessed.</p></div><div><h3>Results</h3><p>Characteristic sonographic features of PAD were identified, including strong echoes (28/30, 93.3 %), strip shape (28/30, 93.3 %), multiple reflections in the posterior echo (27/30, 90.0 %), and location outside the CBD or near the biliary wall in connection with the duodenum (27/30, 90.0 %). Inter-observer agreement was good (<em>Kappa</em> values = 0.69–0.82). Comparative analysis of sonographic features revealed significant differences in echo shape, posterior echo changes (multiple reflections and acoustic shadowing), location and relation to surrounding organs, and intrahepatic and extrahepatic bile duct dilatation status between the dilatation status of the two groups. In particular, these characteristics achieved a sensitivity of 100 % and a specificity of 98 % for the differential diagnosis of PAD and lower CBD stones.</p></div><div><h3>Conclusions</h3><p>This study identified characteristic sonographic features of PAD, which could be used as potential diagnostic indicators to distinguish PAD from lower CBD stones.</p></div>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of low serum 25-Hydroxy vitamin D [25(OH) d] with hepatic encephalopathy in patients with decompensated liver cirrhosis","authors":"Abdul Mumit Sarkar ,&nbsp;Abdullah Al Mukit ,&nbsp;Tanzilul Bari ,&nbsp;Rofiqul Islam ,&nbsp;Shafiqul Islam ,&nbsp;Khalequzzaman Sarker ,&nbsp;Manzurul Chowdhury ,&nbsp;Mohd. Harun Or Rashid ,&nbsp;Abdul Alim","doi":"10.1016/j.ajg.2024.01.014","DOIUrl":"10.1016/j.ajg.2024.01.014","url":null,"abstract":"<div><h3>Background and study aims</h3><p>The mechanism of hepatic encephalopathy is complex and has not been conclusively established. Recent studies support lower serum 25-Hydroxy Vitamin D [25(OH) D] levels in patients with hepatic encephalopathy. This study aimed to evaluate the association between serum 25(OH) D and hepatic encephalopathy in patients with decompensated cirrhosis of liver.</p></div><div><h3>Patients and methods</h3><p>A total of 70 cirrhosis patients (35 cases of hepatic encephalopathy and 35 patients without encephalopathy as control, mean age 53.07 ± 12.99 years, 67 % male) were recruited for this study. Assessment of the severity of cirrhosis was done by using a model for end-stage liver disease(MELD) and Child Turcotte Pugh (CTP) scores, and assessment of the severity of hepatic encephalopathy was done according to West Haven criteria. Serum 25 (OH) D level was measured by Chemiluminescent Microparticle Immuno Assay(CMIA).</p></div><div><h3>Results</h3><p>The mean serum 25(OH) D level among hepatic encephalopathy patients was significantly lower in comparison to the control group without encephalopathy (18.76 ± 8.84 nmol/L vs 31.19 ± 13.9 nmol/L, <em>P</em>&lt;0.0001). 91.4 % of hepatic encephalopathy patients had moderate to severe 25(OH)D deficiency as compared to 51.4 % in the control group. There was a significant correlation observed between the severity of the 25 (OH) D deficiency and the severity of liver disease (r =  − 0.35, <em>P</em> = 0.002). No statistically significant difference in serum 25(OH) D levels was found among patients with different hepatic encephalopathy grades (<em>P</em> = 0.416).</p></div><div><h3>Conclusion</h3><p>A significant association was found between a low serum 25(OH) D leveland hepatic encephalopathy. It requires further large-scale multicenter studies to establish it as a risk factor and predictor of hepatic encephalopathy.</p></div>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of THBS1 and PDGFD in the immune microenvironment of Helicobacter pylori-associated gastric cancer","authors":"Yun Zhou ,&nbsp;Shixiong Liu ,&nbsp;Ya Zheng ,&nbsp;Yuping Wang ,&nbsp;Yongning Zhou","doi":"10.1016/j.ajg.2024.02.001","DOIUrl":"10.1016/j.ajg.2024.02.001","url":null,"abstract":"<div><h3>Background and study aims</h3><p>Immunotherapy has emerged as a hot topic in cancer treatment in recent years and has also shown potential in the treatment of Helicobacter pylori-associated gastric cancer. However, there is still a need to identify potential immunotherapy targets.</p></div><div><h3>Material and methods</h3><p>We used the GSE116312 dataset of Helicobacter pylori-associated gastric cancer to identify differentially expressed genes, which were then overlapped with immune genes from the ImmPort database. The identified immune genes were used to classify gastric cancer samples and evaluate the relationship between classification and tumor mutations, as well as immune infiltration. An immune gene-based prognostic model was constructed, and the expression levels of the genes involved in constructing the model were explored in the tumor immune microenvironment.</p></div><div><h3>Results</h3><p>We successfully identified 60 immune genes and classified gastric cancer samples into two subtypes, which showed differences in prognosis, tumor mutations, immune checkpoint expression, and immune cell infiltration. Subsequently, we constructed an immune prognostic model consisting of THBS1 and PDGFD, which showed significant associations with macrophages and fibroblasts.</p></div><div><h3>Conclusion</h3><p>We identified abnormal expression of THBS1 and PDGFD in cancer-associated fibroblasts (CAFs) within the tumor immune microenvironment, suggesting their potential as therapeutic targets.</p></div>","PeriodicalId":48674,"journal":{"name":"Arab Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}