Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"Effect of Ultra-Low Concentrations of Doxorubicin in Biological Nanoparticles in Breast Tumor Models","authors":"A. D. Yudaeva,&nbsp;N. I. Ponomareva,&nbsp;S. A. Brezgin,&nbsp;A. S. Frolova,&nbsp;A. P. Kostyusheva,&nbsp;P. A. Demina,&nbsp;D. V. Sokolova,&nbsp;G. Babayeva,&nbsp;I. I. Khan,&nbsp;E. V. Khaydukov,&nbsp;A. Parodi,&nbsp;V. S. Pokrovsky,&nbsp;A. A. Zamyatnin, Jr.,&nbsp;V. P. Chulanov,&nbsp;D. S. Kostyushev","doi":"10.1134/S1990750825600293","DOIUrl":"10.1134/S1990750825600293","url":null,"abstract":"<p>To date, chemotherapeutic agents remain the primary method for treating many oncological diseases, including breast cancer (BC). However, numerous side effects caused by the indiscriminate destruction of actively dividing cells limit the safe use of chemotherapeutic agents in clinical practice. Due to the combination of unique properties of nanoparticles, including high biocompatibility and the ability to overcome biological barriers, biological nanoparticles (BNP) are a promising tool for reducing the unwanted toxicity of modern chemotherapeutic agents. This study examined the efficacy of delivering ultra-low concentrations of doxorubicin (0.0544 mg/kg), a dose that is 100 times less than the single therapeutic dose used in a mouse model, using orthotopic and functionalized exosome-like nanoparticles in BC models in vitro and in vivo. It was shown that doxorubicin loaded in BNP exhibited increased cytotoxic activity compared to the free chemotherapeutic agent in vitro. Moreover, a more pronounced activity of functionalized (targeted) nanoparticles towards breast cancer cell receptors was demonstrated compared to orthotopic particles obtained from the tumor. In an in vivo experiment on mice with BC, doxorubicin in ultra-low doses incorporated in BNP did not show an anti-tumor effect; however, there was a trend towards a reduction in tumor nodule size with the administration of orthotopic and functionalized BNP with doxorubicin. Therefore, (1) BNP enhance anti-tumor activity, allowing for a reduced dose of the administered chemotherapeutic agent, (2) the creation of targeted nanoparticles ensures enhanced accumulation of the drug in tumor cells, but (3) ultra-low doses of the chemotherapeutic agent do not have a pronounced effect on BC growth in vivo. The results of the study indicate the potential for using BNP as a strategy to reduce off-target toxicity of modern chemotherapeutic agents.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 4","pages":"395 - 402"},"PeriodicalIF":0.4,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147335741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Hydration and Catalytic Activity of Endonucleases: The Case of Cas9 and Its Evolutionary Variants","authors":"Ariana Delgado,&nbsp;Ysaías J. Alvarado,&nbsp;Alejandro Vivas,&nbsp;Anibal Méndez,&nbsp;Patricia Rodríguez-Lugo,&nbsp;María Elena Troconis,&nbsp;Lenin González-Paz,&nbsp;Yasmir Quiróz,&nbsp;José Luis Paz,&nbsp;Yovani Marrero-Ponce,&nbsp;Felix Martinez-Rios,&nbsp;Joan Vera-Villalobos","doi":"10.1134/S1990750825600967","DOIUrl":"10.1134/S1990750825600967","url":null,"abstract":"<p>This study examined the structures of <i>Sp</i>Cas9 endonuclease of <i>Streptococcus pyogenes</i> and their evolutionary variants using different computational biophysical models to investigate the behavior of hydration in these endonucleases. Although the mechanism of <i>Sp</i>Cas9 is well understood from an evolutionary perspective, its hydration has not been thoroughly explored. The study found that all endonucleases tended to compact together and expose less surface area to water as a solvent, resulting in a significant loss of water molecules from the hydration layer, as occurs in the folding of many globular proteins. A comparative analysis revealed that the distribution of water molecules in the hydration shell and PI domain, which is responsible for the biological recognition function of ligand, differed between each endonuclease. All endonucleases have a higher density in their hydration shell in relation to the density of water as a solvent, with <i>Sp</i>Cas9 having the highest density in the hydration shell (19%) and the lowest being the primitive endonuclease SCA (4%) in relation to the bulk water. The previously reported catalytic activity of these endonucleases toward the OCA2 and TYR genes increased nonlinearly with both maximum of probability density of the number of water molecules <span>({{P}_{{left( {{{n}_{w}}} right)}}})</span> and the degree of hydration <span>(~{{P}_{{left( {{{delta }_{h}}} right)}}})</span> in the evolutionary direction from the oldest to the current. These findings suggest that water molecules in the hydration shell play an important role in the conformational changes, biological recognition, and activity of this endonuclease of great biotechnological interest.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 4","pages":"352 - 363"},"PeriodicalIF":0.4,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147335759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full Transcriptome Analysis of the Liver of Rats Receiving the Complex of L-Carnitine and Resveratrol in the Composition of a Standard or Hypercaloric Diet","authors":"Nikita V. Trusov,&nbsp;Vladimir A. Shipelin,&nbsp;Ivan V. Gmoshinski","doi":"10.1134/S1990750824601322","DOIUrl":"10.1134/S1990750824601322","url":null,"abstract":"<p>The use of resveratrol and L-carnitine (RC) in the complex as components of dietary products and food supplements is considered a potential approach to the dietary therapy of obesity and associated pathologies. However, the interactions between these bioactive substances upon ingestion, particularly their combined effects on global gene expression patterns, remain poorly understood. We investigated the hepatic transcriptome in male Wistar rats fed a control diet (CD) or a high-fat/high-carbohydrate diet (HFCD), supplemented with low (RCl) or high (RCh) dose RC for 64 days. HFCD altered the expression of 757 genes, while HFCD with RC induced more modest changes (179–243 genes). A key finding was a significant negative correlation between gene expression changes induced by HFCD and RC within the HFCD, indicating a compensatory effect of the supplement. Only two genes, <i>Asns</i> and <i>RT1-CE10</i>, responded to both RC doses in both diets. Bioinformatics analysis revealed that RC targeted four metabolic pathways (KEGGs) also disrupted by HFCD: drug and xenobiotic metabolism by cytochrome P450, retinol metabolism, and steroid hormone biosynthesis. The PPAR signaling pathway was altered by both RC doses in CD-fed rats and by RCh in HFCD-fed rats. These findings identify xenobiotic detoxification, retinoid/steroid metabolism, and fatty acid oxidation as key transcriptomic targets through which the RC supplement may counteract diet-induced obesity. The interaction between resveratrol and L-carnitine at the gene expression level should be considered when developing combined dietary supplements.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 4","pages":"369 - 384"},"PeriodicalIF":0.4,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147335828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential for Bioengineering Intervention in Tooth Regeneration","authors":"A. B. Kazumova","doi":"10.1134/S1990750825600475","DOIUrl":"10.1134/S1990750825600475","url":null,"abstract":"<p>In recent decades, bioengineering and regenerative medicine have shown significant progress in restoring lost teeth and tissue. The review is devoted to modern possibilities of bioengineering intervention in tooth regeneration, including advances in the field of stem cells, 3D printing, biomaterials, gene therapy, and tissue engineering. Embryonic mechanisms of tooth formation and key signaling pathways, such as BMP (Bone Morphogenetic Proteins), FGF (Fibroblast Growth Factors), Wnt, and Shh (Sonic Hedgehog), are the basis for the development of new therapeutic strategies. One of the main directions is the use of different types of stem cells, including dental pulp cells (DPSCs), deciduous teeth (SHED), and periodontal cells (PDLSCs), as well as alternative sources, such as mesenchymal stem cells from bone marrow and adipose tissue. An important aspect is the use of innovative materials, such as biodegradable polymers, natural matrices, and nanostructured coatings, to create 3D scaffolds that support tissue growth. Particular attention is paid to molecular methods of intervention, including the use of growth factors (BMP-2, FGF-2, VEGF, TGF-β), gene therapy (e.g., using CRISPR/Cas9), and controlled gene expression, to activate odontogenesis. Prospects for creating teeth are being considered de novo and with dental organoids as are problems associated with the integration of regenerated tissues into the body, including issues of vascularization, innervation, and immunocompatibility. Important issues remain control over directed cell differentiation, the durability of regenerated teeth, and the ethical and legal aspects of using genetic engineering. Translation of these methods into clinical practice requires the integration of bioengineering, bioinformatics, materials science, and clinical dentistry as well as the development of clinical trials and appropriate legal regulation. In the coming decades, dental regeneration may become an important element of personalized medicine, which will require further improvement of technologies, testing of new biomaterials and more precise control of cellular processes, which will ultimately ensure the successful implementation of this approach in clinical practice.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"230 - 243"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myoblast Communication and Antibacterial Potential of Poly(lactic acid) Nanoscaffolds: Advancing Musculoskeletal Tissue Engineering Implants","authors":"Touseef Amna","doi":"10.1134/S199075082560027X","DOIUrl":"10.1134/S199075082560027X","url":null,"abstract":"<p>Myogenic bioengineering has emerged as a promising field aimed at repairing or regenerating damaged muscle tissues triggered by trauma, diseases, or congenital defects. Poly(lactic acid) (PLA) nanofibrous scaffolding has appealed to progressive attraction as an innovative support for prospective therapy in renewal/or repair medicine. Herein, high aspect ratio PLA nanofibers were fabricated using electrospinning and characterized by means of various sophisticated techniques such as XRD, SEM, TGA and FTIR spectroscopy. There is no information regarding the interaction of muscle cells with PLA. Thus, muscle precursor cells were exposed to various doses of electrospun PLA nanoscaffolds to test proliferation and in vitro biocompatibility. Cell viability was assessed by CCK-8 at standard time periods. The structure of treated myoblasts was scanned by a phase contrast microscope and to check the growing profile Bio-SEM was used. Furthermore, antimicrobial activity was explored against Gram-positive <i>Staphylococcus aureus</i>. The findings specify that PLA nanoscaffolds exhibit mild antimicrobial efficiency. Besides, the myoblasts demonstrated proliferative behavior on nanoscaffolds. Our investigation highlights that PLA nanofibers hold significant promise for developing biomimetic cellular scaffolds. Moreover, the PLA nanoscaffolds developed in this study show great potential for tissue engineering, particularly in muscle regeneration. Its high biocompatibility and ability to support myoblast attachment, proliferation and differentiation make it a promising biomaterial implant for regenerative medicine.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"305 - 315"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anemia of Chronic Renal Failure and ESRD with Hepatitis C Patients: Role of Erythropoietin","authors":"Marwa Adnan Jabbar,&nbsp; Jawad Kadhum Muraih","doi":"10.1134/S1990750824600390","DOIUrl":"10.1134/S1990750824600390","url":null,"abstract":"<p>Chronic kidney disease (CKD) is a serious global public health concern, affecting around 13% of the population. Anemia represents a prevalent consequence of CKD. Anemia improves the probability of cardiovascular disease, accelerates the course of kidney failure, and reduces life quality. Patients with CKD can develop anemia for a variety of reasons, which include but are not limited to lack of renal function. These individuals need a thorough assessment to determine and treat causes of anemia besides erythropoietin insufficiency. However, there is evidence of a lack of circulating erythropoietin (EPO). In this study, we evaluated serum EPO levels in 41 severely anemic individuals with HGB below 7 g/dL, including 184 ESRD patients with and without hepatitis C. The ESRD group with severe anemia showed serum EPO levels of 1.3–1.7 pg/mL, whereas the ESRD group with HCV had an EPO level of 1.0–1.2 pg/mL. Consider using recombinant human erythropoietin (rHuEPO) to treat the condition.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"288 - 295"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KMT2A Gene Rearrangements Assessment in Molecular Diagnostics of Acute Leukemia and Lymphoma","authors":"E. Zerkalenkova,&nbsp;E. Mikhailova,&nbsp;S. Lebedeva,&nbsp;A. Popov,&nbsp;N. Lomov,&nbsp;M. Rubtsov","doi":"10.1134/S1990750825600165","DOIUrl":"10.1134/S1990750825600165","url":null,"abstract":"<p><i>KMT2A</i> gene rearrangements are common genetic events in acute leukemia and lymphoma, particularly in younger patients, and are associated with poor outcome. Due to the wide range of partner genes and breakpoint locations, accurate identification of <i>KMT2A</i> rearrangements can be challenging in clinical molecular diagnostics. To achieve a rapid and cost-effective data on <i>KMT2A</i> translocation partner and fusion gene sequence, we follow a structured approach that includes standard GTG-banded karyotyping, fluorescence in situ hybridization (FISH) with 11q23.3 break-apart or translocation probes, DNA-based next-generation sequencing (NGS) for the entire <i>KMT2A</i> gene, and subsequent confirmation of fusion gene expression using real-time PCR with reverse transcription. Here, we illustrate our diagnostic workflow with a case of pediatric T-cell lymphoma harboring a <i>KMT2A</i> gene rearrangement.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"296 - 304"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Determination, Engineering Demonstration, and Preliminary Docking Studies of Potential Antimicrobial Peptides from Cocosin-1 against Methicillin-Resistant Staphylococcus aureus (MRSA) Muramyl E (MurE) Ligase","authors":"Driyah Nicole E. Dario,&nbsp;Kuven A. Malig-on,&nbsp;Anna Fatima M. Perea,&nbsp;Nedrick T. Distor","doi":"10.1134/S1990750825600499","DOIUrl":"10.1134/S1990750825600499","url":null,"abstract":"<p>The alarming rise of antibiotic resistance triggered the search for antimicrobial alternatives such as plant-based antimicrobial peptides (AMPs) since they possess a wide range of bioactivities. Herein, we evaluated the antimicrobial probability of peptides from enzyme-digested cocosin-1, improved the antimicrobial probability of selected peptides through engineering, and investigated possible peptide-enzyme interactions. Cocosin-1 was individually digested using pepsin, trypsin, and chymotrypsin, generating AMPs which were analyzed for their antimicrobial probabilities. This approach yielded 9 (CAMPR3) and 25 AMPs (Deep-AmPEP30). The top three peptides with the highest antimicrobial probabilities produced per enzyme were subjected to site-specific amino acid (histidine) substitution while maintaining the conserved sequences intact. The physicochemical properties of the top five engineered peptides were assessed to evaluate their potential efficacy. To understand their mechanisms of action, the original and engineered AMPs with the highest antimicrobial probabilities were modeled and docked against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) MurE ligase, a bacterial enzyme critical for cell wall biosynthesis. Upon histidine substitution, peptide C1DP-C1 (¹²⁹QRSEREEGERHRW¹⁴¹) displayed the highest antimicrobial probability (82.45%) while peptide CIDP-P2 (³³QSPRRSVSSRNECRIERL⁵⁰) showed the most significant antimicrobial probability improvement (31.70%). Docking studies revealed essential binding interactions, including salt bridges and hydrogen bonding, which may be crucial in AMP-based enzyme inhibition. Overall, this study provides a foundational idea that site-specific changes in the amino acid composition may affect and improve the antimicrobial activity of selected peptides for potential antibacterial therapeutics.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"244 - 257"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Schiff Base Clubbed Methylene Derivatives: Design, Synthesis, Biological Screening, Molecular Docking and Pharmacokinetic Studies","authors":"Jigisha Modi,&nbsp;Rahul Tailor,&nbsp;Savan Patel,&nbsp;Rucha Wani,&nbsp;Merwyn Dcosta,&nbsp;Arvind Singh","doi":"10.1134/S1990750824600432","DOIUrl":"10.1134/S1990750824600432","url":null,"abstract":"<p>A novel series of 4,4'-methylene bis(<i>N</i>-substituted benzylidene-2-fluoro aniline) derivatives (SE₁ to SE₁₀) was synthesized through the condensation reaction of a 4,4'-methylene bis(2-fluoro aniline) with arious substituted aldehydes. The structural characterization of these compounds was carried out using FTIR, ¹H-NMR, and mass spectrometry techniques. The antimicrobial and antifungal potential of the synthesized compounds was evaluated using the serial dilution method. Among them, SE₃ and SE₉ demonstrated notable antibacterial activity when compared to standard reference drugs, while SE₂ and SE₅ showed good antifungal effects. To investigate the interaction of the synthesized molecules with biological targets, molecular docking studies were performed on all ten compounds. Lanosterol 14-alpha demethylase (PDB ID: 5V5Z) and carbonic anhydrase II (PDB ID: 7SEV) were selected as the target proteins for these studies. Compounds SE₁₀, SE₁, SE₂, and SE₅ exhibited strong binding affinities toward lanosterol 14-alpha demethylase, with docking scores of –11.608, –11.53, –11.485 and –11.386 kcal/mol, respectively. Meanwhile, SE₁ and SE₃ showed the highest binding affinity for Carbonic anhydrase II, with a docking score of –9.504 and –9.37 kcal/mol respectively. Additionally, the physicochemical properties of all synthesized compounds were assessed and reported.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"267 - 278"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bile Acid Profiling for Differential Diagnosis of Hepatobiliary Cancers and Cholelitiasis","authors":"G. S. Stupnikova,&nbsp;A. D. Dolbnya,&nbsp;A. A. Kuzin,&nbsp;I. A. Popov,&nbsp;S. I. Pekov","doi":"10.1134/S1990750823600449","DOIUrl":"10.1134/S1990750823600449","url":null,"abstract":"<p>Cholangiocarcinoma (CCA) and pancreatic ductal adenocarcinoma (PDAC) are malignant tumors that can be characterized by low patient survival rates. They have similar anatomical proximity, morphological similarity, and an overlapping immunohistochemical profile. Even with the aid of modern imaging techniques, it is challenging to differentiate PDAC from distal CCA, as no specific biomarkers have been discovered. Bile is a promising material for research due to the possibility of relatively minimally invasive collection of biomaterial as well as direct contact with the tumor. The purpose of this work is to identify the predominant bile acids for each disease, which will help with the differential diagnosis of PDAC and CCA, while using bile from patients with cholelithiasis as a nontumor control. Bile acids were isolated from the bile of patients with three selected pathologies and studied by tandem chromatography−mass spectrometry. In the bile of patients with cholelithiasis, there are found a variety of isomers of deoxycholic acid as well as an increased proportion of glycolithocholic, glycodeoxycholic, and taurodeoxycholic acids. A predominance of taurocholic acid isomers and an increase in the proportion of glycoursodeoxycholic acid in the bile of patients with CCA is observed. Glycocholic acid is the predominant bile acid in patients with PDAC. Based on the obtained data, a set of bile acids was chosen, making it possible to compile a panel of biomarkers for the differentiation of cholelithiasis, PDAC and CCA.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 3","pages":"258 - 266"},"PeriodicalIF":0.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145442819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}