Journal of Integrative Medicine-Jim最新文献

{"title":"Alisol B 23-acetate promotes white adipose tissue browning to mitigate high-fat diet-induced obesity by regulating mTOR-SREBP1 signaling","authors":"Lu-lu Han , Xin Zhang , Hui Zhang , Ting Li , Yi-chen Zhao , Ming-hui Tian , Feng-lei Sun , Bo Feng","doi":"10.1016/j.joim.2024.01.003","DOIUrl":"10.1016/j.joim.2024.01.003","url":null,"abstract":"<div><h3>Objective</h3><p>Obesity is a global health concern with management strategies encompassing bariatric surgery and anti-obesity drugs; however, concerns regarding complexities and side effects persist, driving research for more effective, low-risk strategies. The promotion of white adipose tissue (WAT) browning has emerged as a promising approach. Moreover, alisol B 23-acetate (AB23A) has demonstrated efficacy in addressing metabolic disorders, suggesting its potential as a therapeutic agent in obesity management. Therefore, in this study, we aimed to investigate the therapeutic potential of AB23A for mitigating obesity by regulating metabolic phenotypes and lipid distribution in mice fed a high-fat diet (HFD).</p></div><div><h3>Methods</h3><p>An obesity mouse model was established by administration of an HFD. Glucose and insulin metabolism were assessed via glucose and insulin tolerance tests. Adipocyte size was determined using hematoxylin and eosin staining. The expression of browning markers in WAT was evaluated using Western blotting and quantitative real-time polymerase chain reaction. Metabolic cage monitoring involved the assessment of various parameters, including food and water intake, energy metabolism, respiratory exchange rates, and physical activity. Moreover, oil red O staining was used to evaluate intracellular lipid accumulation. A bioinformatic analysis tool for identifying the molecular mechanisms of traditional Chinese medicine was used to examine AB23A targets and associated signaling pathways.</p></div><div><h3>Results</h3><p>AB23A administration significantly reduced the weight of obese mice, decreased the mass of inguinal WAT, epididymal WAT, and perirenal adipose tissue, improved glucose and insulin metabolism, and reduced adipocyte size. Moreover, treatment with AB23A promoted the expression of browning markers in WAT, enhanced overall energy metabolism in mice, and had no discernible effect on food intake, water consumption, or physical activity. In 3T3-L1 cells, AB23A inhibited lipid accumulation, and both AB23A and rapamycin inhibited the mammalian target of rapamycin-sterol regulatory element-binding protein-1 (mTOR-SREBP1) signaling pathway. Furthermore, 3-isobutyl-1-methylxanthine, dexamethasone and insulin, at concentrations of 0.25 mmol/L, 0.25 μmol/L and 1 μg/mL, respectively, induced activation of the mTOR-SREBP1 signaling pathway, which was further strengthened by an mTOR activator MHY1485. Notably, MHY1485 reversed the beneficial effects of AB23A in 3T3-L1 cells.</p></div><div><h3>Conclusion</h3><p>AB23A promoted WAT browning by inhibiting the mTOR-SREBP1 signaling pathway, offering a potential strategy to prevent obesity.</p><p>Please cite this article as: Han LL, Zhang X, Zhang H, Li T, Zhao YC, Tian MH, Sun FL, Feng B. Alisol B 23-acetate promotes white adipose tissue browning to mitigate high-fat diet-induced obesity by regulating mTOR-SREBP1 signaling. <em>J Integr Med</em>. 2024; 22(1): 83–92.</","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"22 1","pages":"Pages 83-92"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095496424000037/pdfft?md5=aa5eacc46ad058398ada8236b7b97dab&pid=1-s2.0-S2095496424000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139554766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency","authors":"Jing-shun Meng ,&nbsp;Yun He ,&nbsp;Heng-bin Yang ,&nbsp;Li-ping Zhou ,&nbsp;Si-yuan Wang ,&nbsp;Xi-lin Feng ,&nbsp;Omar Yahya Al-shargi ,&nbsp;Xiao-min Yu ,&nbsp;Li-qing Zhu ,&nbsp;Chang-quan Ling","doi":"10.1016/j.joim.2024.01.001","DOIUrl":"10.1016/j.joim.2024.01.001","url":null,"abstract":"<div><h3>Objective</h3><p><span><span>Melittin<span> and its derivative have been developed to support effective gene delivery systems. Their ability to facilitate endosomal release enhances the delivery of nanoparticle-based gene therapy. Nevertheless, its potential application in the context of viral vectors has not received much attention. Therefore, we would like to optimize the </span></span>rAAV vector<span> by Melittin analog to improve the transduction efficiency of rAAV in liver </span></span>cancer cells and explore the mechanism of Melittin analog on rAAV.</p></div><div><h3>Methods</h3><p><span>Various melittin-derived peptides were inserted into loop VIII of the capsid protein in recombinant adeno-associated virus vectors. These vectors carrying either </span><span><em>gfp</em></span> or <em>fluc</em><span><span> genes were subjected to quantitative polymerase chain reaction assays and transduction assays in human embryonic kidney 293 (HEK293T) cells to investigate the efficiency of vector production and gene delivery. In addition, the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo </span>tail vein<span> administration to C57BL/6 mice. Finally, the intricate details of the vector-mediated transduction mechanisms were explored by using pharmacological inhibitors of every stage of the rAAV2 intracellular life cycle.</span></span></p></div><div><h3>Results</h3><p>A total of 76 melittin-related peptides were identified from existing literature. Among them, CMA-3, p5RHH and aAR3 were found to significantly inhibit transduction of rAAV2 vector crude lysate. The p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to rAAV. Mechanistically, bafilomycin A1<span>, a vacuolar endosome acidification inhibitor, completely inhibited the transgene expression mediated by the p5RHH-rAAV2 vectors. Most importantly, p5RHH-rAAV8 vectors also increased hepatic transduction in vivo in C57BL/6 mice.</span></p></div><div><h3>Conclusion</h3><p>The incorporation of melittin analogs into the rAAV capsids results in a significant improvement in rAAV-mediated transgene expression. While further modifications remain an area of interest, our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.</p><p>Please cite this article as: Meng J, He Y, Yang H, Zhou L, Wang S, Feng X, Al-shargi OY, Yu X, Zhu L, Ling, C. Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency. <em>J Integr Med.</em> 2024; 22(1): 72–82.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"22 1","pages":"Pages 72-82"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139499619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting whole person health: Exploring the role of traditional Chinese medicine in Polish healthcare","authors":"Monika Rybicka ,&nbsp;Jing Zhao ,&nbsp;Karolina Piotrowicz ,&nbsp;Sara Ptasnik ,&nbsp;Kamila Mitka ,&nbsp;Magdalena Kocot-Kępska ,&nbsp;Ka-Kit Hui","doi":"10.1016/j.joim.2023.10.001","DOIUrl":"10.1016/j.joim.2023.10.001","url":null,"abstract":"<div><p>Poland has a unique history of traditional Chinese medicine (TCM) dating back to the 17th century when Polish missionary Michael (Michał) Boym was a pioneer in the field. In the 20th century, his successor, Professor Zbigniew Garnuszewski, reintroduced acupuncture to medical practice in Poland. However, other methods of TCM and its holistic approach to patient care have not found their place in modern medicine in Poland. At present, the legal status of TCM in Poland remains unregulated, with TCM included in the broad spectrum of complementary and alternative medicine (CAM) practices. Few reports are available on the use of TCM methods among the Polish population. Integrative medicine combines conventional medicine with evidence-based CAM interventions and considers all aspects of a patient’s health, including physical, emotional, mental, social, and environmental factors. An integrative healthcare model that incorporates TCM modalities and lifestyle recommendations as well as a whole person approach may provide a more sustainable solution for the constantly underfinanced Polish healthcare system, which faces challenges of multimorbidity in an aging society and limited access to care. The coronavirus disease 2019 pandemic, war in Ukraine, and ongoing climate crisis have underscored the need to strengthen the resilience of the Polish healthcare system and search for new solutions. A model of care that blends the best of biomedicine and TCM healing approaches may be a better option for both patients and the healthcare system in Poland.</p><p>Please cite this article as: Rybicka M, Zhao J, Piotrowicz K, Ptasnik S, Mitka K, Kocot-Kępska M, Hui KK. Promoting whole person health: Exploring the role of traditional Chinese medicine in Polish healthcare. <em>J Integr Med</em>. 2023; 21(6): 509–517.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 509-517"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S209549642300078X/pdfft?md5=086c053321e44bbff6189eac9f60a87a&pid=1-s2.0-S209549642300078X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71482501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiaotan Sanjie recipe, a compound Chinese herbal medicine, inhibits gastric cancer metastasis by regulating GnT-V-mediated E-cadherin glycosylation","authors":"Nian Huang ,&nbsp;Hai-wei He ,&nbsp;Yu-yu He ,&nbsp;Wei Gu ,&nbsp;Ming-juan Xu ,&nbsp;Long Liu","doi":"10.1016/j.joim.2023.11.001","DOIUrl":"10.1016/j.joim.2023.11.001","url":null,"abstract":"<div><h3>Objective</h3><p>Xiaotan Sanjie recipe (XTSJ), a Chinese herbal compound medicine, exerts a significant inhibitory effect on gastric cancer (GC) metastasis. This work investigated the mechanism underlying the XTSJ-mediated inhibition of GC metastasis.</p></div><div><h3>Methods</h3><p>The effect of XTSJ on GC metastasis and the associated mechanism were investigated <em>in vitro</em><span>, using GC cell lines, and </span><em>in vivo</em>, using a GC mouse model, by focusing on the expression of Glc-N-Ac-transferase V (GnT-V; encoded by <em>MGAT5</em>).</p></div><div><h3>Results</h3><p><span>The migration and invasion ability of GC cells decreased significantly after XTSJ administration, which confirmed the efficacy of XTSJ in treating GC </span><em>in vitro</em>. XTSJ increased the accumulation of E-cadherin at junctions between GC cells, which was reversed by <em>MGAT5</em> overexpression. XTSJ administration and <em>MGAT5</em> knockdown alleviated the structural abnormality of the cell–cell junctions, while <em>MGAT5</em> overexpression had the opposite effect. <em>MGAT5</em><span><span> knockdown and XTSJ treatment also significantly increased the accumulation of proteins associated with the E-cadherin-mediated </span>adherens junction complex. Furthermore, the expression of </span><em>MGAT5</em> was significantly lower in the lungs of BGC-823-<em>MGAT5</em> + XTSJ mice than in those of BGC-823-<em>MGAT5</em> + solvent mice, indicating that the ability of gastric tumors to metastasize to the lung was decreased <em>in vivo</em> following XTSJ treatment.</p></div><div><h3>Conclusion</h3><p>XTSJ prevented GC metastasis by inhibiting the GnT-V-mediated E-cadherin glycosylation and promoting the E-cadherin accumulation at cell–cell junctions.</p><p><span><span>Please cite this article as: Huang N, He HW, He YY, Gu W, Xu MJ, Liu L. Xiaotan Sanjie recipe, a compound Chinese herbal medicine, inhibits gastric </span>cancer metastasis by regulating GnT-V-mediated E-cadherin glycosylation. </span><em>J Integr Med</em>. 2023; 21(6): 561–574.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 561-574"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135510098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of Kangxian Huanji Granule as adjunctive treatment in acute exacerbation of idiopathic pulmonary fibrosis: An exploratory randomized controlled trial","authors":"Jian-sheng Li ,&nbsp;Hai-long Zhang ,&nbsp;Wen Guo ,&nbsp;Lu Wang ,&nbsp;Dong Zhang ,&nbsp;Li-min Zhao ,&nbsp;Miao Zhou","doi":"10.1016/j.joim.2023.11.003","DOIUrl":"10.1016/j.joim.2023.11.003","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;Acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is an important occurrence in the natural history of idiopathic pulmonary fibrosis (IPF), associated with high hospitalization rates, high mortality and poor prognosis. At present, there is no effective treatment for AE-IPF. Chinese herbal medicine has some advantages in treating IPF, but its utility in AE-IPF is unclear.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;p&gt;The treatment of AE-IPF with Kangxian Huanji Granule (KXHJ), a compound Chinese herbal medicine, lacks an evidence-based justification. This study explores the efficacy and safety of KXHJ in patients with AE-IPF.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Design, setting, participants and interventions&lt;/h3&gt;&lt;p&gt;We designed a randomized, double-blind, placebo-controlled, exploratory clinical trial. A total of 80 participants diagnosed with AE-IPF were randomly assigned to receive KXHJ or a matching placebo; the treatment included a 10 g dose, administered twice daily for 4 weeks, in addition to conventional treatment. Participants were followed up for 12 weeks after the treatment.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Main outcome measures&lt;/h3&gt;&lt;p&gt;The primary endpoints were treatment failure rate and all-cause mortality. Secondary endpoints included the length of hospitalization, overall survival, acute exacerbation rate, intubation rate, the modified British Medical Research Council (mMRC) score, and the St George’s Respiratory Questionnaire for IPF (SGRQ-I) score.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;The rate of treatment failure at 4 weeks was lower in the intervention group compared to the control group (risk ratio [RR]: 0.22; 95% confidence interval [CI]: 0.051 to 0.965, &lt;em&gt;P&lt;/em&gt; = 0.023). There was no significant difference in all-cause mortality at 16 weeks (RR: 0.75; 95% CI: 0.179 to 3.138; &lt;em&gt;P&lt;/em&gt; &gt; 0.999) or in the acute exacerbation rate during the 12-week follow-up period (RR: 0.69; 95% CI: 0.334 to 1.434; &lt;em&gt;P&lt;/em&gt; = 0.317). The intervention group had a shorter length of hospitalization than the control group (mean difference [MD]: –3.30 days; 95% CI, –6.300 to –0.300; &lt;em&gt;P&lt;/em&gt; = 0.032). Significant differences in the mean change from baseline in the mMRC (between-group difference: –0.67; 95% CI: –0.89 to –0.44; &lt;em&gt;P&lt;/em&gt; &lt; 0.001) and SGRQ-I score (between-group difference: –10.36; 95% CI: –16.483 to –4.228; &lt;em&gt;P&lt;/em&gt; = 0.001) were observed after 4 weeks, and also in the mMRC (between-group difference: –0.67; 95% CI: –0.91 to –0.43; &lt;em&gt;P&lt;/em&gt; &lt; 0.001) and SGRQ-I (between-group difference: –10.28; 95% CI, –15.838 to –4.718; &lt;em&gt;P&lt;/em&gt; &lt; 0.001) at 16 weeks. The difference in the adverse events was not significant.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;KXHJ appears to be effective and safe for AE-IPF and can be considered a complementary treatment in patients with AE-IPF. As a preliminary exploratory study, our results provide a basis for further clinical research.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Trial registration&lt;/h3&gt;&lt;p&gt;Chinese Clinical Tria","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 543-549"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095496423000845/pdfft?md5=8b69c50f6ff985032d54df985795edab&pid=1-s2.0-S2095496423000845-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135515567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal combinations against COVID-19: A network pharmacology, molecular docking and dynamics study","authors":"Jian-bo Song ,&nbsp;Li-qin Zhao ,&nbsp;Hong-ping Wen ,&nbsp;Yuan-ping Li","doi":"10.1016/j.joim.2023.09.001","DOIUrl":"10.1016/j.joim.2023.09.001","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of this study is to identify molecules from traditional Chinese medicine<span> (TCM) with potential activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants.</span></p></div><div><h3>Methods</h3><p><span><span>We applied the Apriori algorithm to identify important combinations of herbs in the TCM prescriptions for the treatment of </span>coronavirus disease 2019 (COVID-19). Then, we explored the active components and core targets using network pharmacology. In addition, the </span>molecular docking approach was performed to investigate the interaction of these components with the main structural and non-structural proteins, as well as the mutants. Furthermore, their stability in the binding pockets was further evaluated with the molecular dynamics approach.</p></div><div><h3>Results</h3><p>A combination of <em>Amygdalus Communis</em> Vas., <span><em>Ephedra</em><em> Herba</em></span> and <span><em>Scutellaria baicalensis</em></span><span><span><span> Georgi was selected as the important herbal combination, and 11 main components and 20 core targets against COVID-19 were obtained. These components, including luteolin, </span>naringenin, </span>stigmasterol<span><span>, baicalein, and so on, were the potentially active compounds against COVID-19. The </span>binding affinity of these compounds with the potential targets was as high as the positive controls. Among them, baicalein could interfere with multiple targets simultaneously, and it also interfered with the interaction between spike protein and angiotensin-converting enzyme 2 receptor. Additionally, almost all the systems reached stability during dynamics simulation.</span></span></p></div><div><h3>Conclusion</h3><p>The combination of <em>A</em>. c<em>ommunis</em>, <em>Ephedra Herba</em> and <em>S</em>. <em>baicalensis</em> was the most important herbal combination for the treatment of COVID-19. Baicalein may be a potential candidate against SARS-CoV-2 and its variants.</p><p>Please cite this article as: Song JB, Zhao LQ, Wen HP, Li YP. Herbal combinations against COVID-19: A network pharmacology, molecular docking and dynamics study. <em>J Integr Med</em>. 2023;21(6):593–604.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 593-604"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41140082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory effects of acupuncture in the treatment of chronic obstructive pulmonary disease","authors":"Lin-hong Jiang ,&nbsp;Pei-jun Li ,&nbsp;Ying-qi Wang ,&nbsp;Mei-ling Jiang ,&nbsp;Xiao-yu Han ,&nbsp;Yi-die Bao ,&nbsp;Xin-liao Deng ,&nbsp;Wei-bing Wu ,&nbsp;Xiao-dan Liu","doi":"10.1016/j.joim.2023.11.005","DOIUrl":"10.1016/j.joim.2023.11.005","url":null,"abstract":"<div><p>Numerous randomised controlled trials have suggested the positive effects of acupuncture on chronic obstructive pulmonary disease (COPD). However, the underlying therapeutic mechanisms of acupuncture for COPD have not been clearly summarized yet. Inflammation is central to the development of COPD. In this review, we elucidate the effects and underlying mechanisms of acupuncture from an anti-inflammatory perspective based on animal studies. Cigarette smoke combined with lipopolysaccharide is often used to establish animal models of COPD. Electroacupuncture can be an effective intervention to improve inflammation in COPD, and Feishu (BL13) and Zusanli (ST36) can be used as basic acupoints in COPD animal models. Different acupuncture types can regulate different types of inflammatory cytokines; meanwhile, different acupuncture types and acupoint options have similar effects on modulating the level of inflammatory cytokines. In particular, acupuncture exerts anti-inflammatory effects by inhibiting the release of inflammatory cells, inflammasomes and inflammatory cytokines. The main underlying mechanism through which acupuncture improves inflammation in COPD is the modulation of relevant signalling pathways: nuclear factor-κB (NF-κB) (e.g., myeloid differentiation primary response 88/NF-κB, toll-like receptor-4/NF-κB, silent information regulator transcript-1/NF-κB), mitogen-activated protein kinase signalling pathways (extracellular signal-regulated kinase 1/2, p38 and c-Jun NH2-terminal kinase), cholinergic anti-inflammatory pathway, and dopamine D2 receptor pathway. The current synthesis will be beneficial for further research on the effect of acupuncture on COPD inflammation.</p><p>Please cite this article as: Jiang LH, Li PJ, Wang YQ, Jiang ML, Han XY, Bao YD, Deng XL, Wu WB, Liu XD. Anti-inflammatory effects of acupuncture in the treatment of chronic obstructive pulmonary disease. <em>J Integr Med</em>. 2023; 21(6): 518–527.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 518-527"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095496423000869/pdfft?md5=c9881d77e4efbcbcf80515e2673382b8&pid=1-s2.0-S2095496423000869-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9","authors":"Ya Zhong ,&nbsp;Bo-wen Zhang ,&nbsp;Jin-tao Li ,&nbsp;Xin Zeng ,&nbsp;Jun-xia Pei ,&nbsp;Ya-mei Zhang ,&nbsp;Yi-xi Yang ,&nbsp;Fu-lun Li ,&nbsp;Yu Deng ,&nbsp;Qi Zhao","doi":"10.1016/j.joim.2023.11.004","DOIUrl":"10.1016/j.joim.2023.11.004","url":null,"abstract":"<div><h3>Objective</h3><p>To explore whether the ethanol extract of <em>Herpetospermum caudigerum</em> Wall (EHC), a Xizang medicinal plant traditionally used for treating liver diseases, can improve imiquimod-induced psoriasis-like skin inflammation.</p></div><div><h3>Methods</h3><p><span>Immunohistochemistry<span> and immunofluorescence staining were used to determine the effects of topical EHC use </span></span><em>in vivo</em><span><span> on the skin pathology of imiquimod-induced </span>psoriasis in mice. The protein levels of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-17A (IL-17A) in mouse skin samples were examined using immunohistochemical staining. </span><em>In vitro</em><span><span>, IFN-γ-induced HaCaT<span> cells with or without EHC treatment<span><span> were used to evaluate the expression of keratinocyte-derived intercellular cell adhesion molecule-1 (ICAM-1) and chemokine CXC ligand 9 (CXCL9) using </span>Western blotting and reverse transcription-quantitative polymerase chain reaction. The </span></span></span>protein synthesis inhibitor<span> cycloheximide<span><span> and proteasome inhibitor </span>MG132 were utilized to validate the EHC-mediated mechanism underlying degradation of ICAM-1 and CXCL9.</span></span></span></p></div><div><h3>Results</h3><p>EHC improved inflammation in the imiquimod-induced psoriasis mouse model and reduced the levels of IFN-γ, TNF-α, and IL-17A in psoriatic lesions. Treatment with EHC also suppressed ICAM-1 and CXCL9 in epidermal keratinocytes. Further mechanistic studies revealed that EHC suppressed keratinocyte-derived ICAM-1 and CXCL9 by promoting ubiquitin–proteasome-mediated protein degradation rather than transcriptional repression. Seven primary compounds including ehletianol C, dehydrodiconiferyl alcohol, herpetrione, herpetin, herpetotriol, herpetetrone and herpetetrol were identified from the EHC using ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry.</p></div><div><h3>Conclusion</h3><p><span>Topical application of EHC ameliorates psoriasis-like </span>skin symptoms and improves the inflammation at the lesion sites.</p><p>Please cite this article as: Zhong Y, Zhang BW, Li JT, Zeng X, Pei JX, Zhang YM, Yang YX, Li FL, Deng Y, Zhao Q. Ethanol extract of <em>Herpetospermum caudigerum</em> Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9. <em>J Integr Med</em>. 2023; 21(6): 584–592.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 584-592"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135670377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical pathway for integrative medicine in the treatment of functional constipation in Hong Kong, China","authors":"Dong-jue Wei ,&nbsp;Hui-juan Li ,&nbsp;Zi-pan Lyu ,&nbsp;Ai-ping Lyu ,&nbsp;Zhao-xiang Bian ,&nbsp;Linda LD Zhong","doi":"10.1016/j.joim.2023.11.002","DOIUrl":"10.1016/j.joim.2023.11.002","url":null,"abstract":"<div><h3>Objective</h3><p><span>Functional constipation (FC) is a common </span>intestinal disease<span><span> worldwide. Despite the presence of criteria such as Roman IV, there is no standardized diagnosis and treatment algorithm in Hong Kong that combines both Western and </span>Chinese medicine approaches. This study integrates current effective and safe diagnosis and treatment methods for FC and provides a clear and scientific pathway for clinical professionals and patients.</span></p></div><div><h3>Methods</h3><p>A systematic search of the PubMed, Cochrane Library, and China National Knowledge Infrastructure databases was performed from their inception to June 30th, 2022, collecting the current evidence about the efficacious integrative management for FC. We organized a meeting of professionals in fields relevant to treatment and management of FC to develop a consensus agreement on clinical pathway process.</p></div><div><h3>Results</h3><p>We developed a clinical pathway for the treatment of FC based on the most recent published guidelines and consultation with experts. This pathway includes a hierarchy of recommendations for every step of the clinical process, including clinical intake, diagnostic examination, recommended labs, diagnostic flowchart, and guidance for selection of therapeutic drugs.</p></div><div><h3>Conclusion</h3><p>This pathway establishes clinical standards for the diagnosis and treatment of FC using Chinese medicine and Western medicine; it will help to provide high-quality medical services in Hong Kong for patients with FC.</p><p><span>Please cite this article as: Wei DJ, Li HJ, Lyu ZP, Lyu AP, Bian ZX, Zhong LL. A clinical pathway for integrative medicine in the treatment of functional constipation in Hong Kong, China. </span><em>J Integr Med</em>. 2023; 21(6): 550–560.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 550-560"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135510212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aconite aqueous extract inhibits the growth of hepatocellular carcinoma through CCL2-dependent enhancement of natural killer cell infiltration","authors":"Kang-di Yang,&nbsp;Xu Zhang,&nbsp;Ming-cong Shao,&nbsp;Li-na Wang","doi":"10.1016/j.joim.2023.10.002","DOIUrl":"10.1016/j.joim.2023.10.002","url":null,"abstract":"<div><h3>Objective</h3><p>Aconite is a traditional Chinese herbal medicine that has been found to inhibit the development of liver cancer; however, its exact molecular mechanisms in this process remain unclear. This study explores how aconite aqueous extract (AAE) inhibits hepatocellular carcinoma (HCC).</p></div><div><h3>Methods</h3><p>An <em>in vivo</em><span><span><span> mouse model of subcutaneous liver cancer was established. After AAE treatment, </span>immunohistochemistry (IHC) was used to determine the effect of AAE on natural killer (NK) cells. Subsequently, C57BL/6 mice were used to establish the subcutaneous tumor model, and a group of these mice were treated with anti-PK163 antibody to remove NK cells, which was verified by flow cytometry and IHC. The effect of AAE on the proliferation of </span>HCC cells </span><em>in vitro</em><span> was determined using cell counting kit-8. The effect of AAE on chemokine<span> production in HCC cells was measured using real-time quantitative polymerase chain reaction and an enzyme-linked immunosorbent assay. The effect of AAE on the migration of NK cells was determined using a transwell assay. Finally, the molecular mechanism was investigated using the Western blotting method.</span></span></p></div><div><h3>Results</h3><p>We demonstrated that the ability of AAE to induce overexpression of the cytokine C–C motif chemokine ligand 2 (CCL2) in HCC cells is fundamental to the infiltration of NK cells into the tumor bed. Mechanistically, we found that the upregulation of CCL2 was achieved by the activation of c-Jun N-terminal kinase but not extracellular regulated protein kinase or p38.</p></div><div><h3>Conclusion</h3><p>Our findings suggest that AAE can be used as an effective immune adjuvant to enhance antitumor immunity by increasing NK cell infiltration into tumors, which could help to improve the efficacy of HCC treatments.</p><p>Please cite this article as: Yang KD, Zhang X, Shao MC, Wang LN. Aconite aqueous extract inhibits the growth of hepatocellular carcinoma through CCL2-dependent enhancement of natural killer cell infiltration. <em>J Integr Med</em>. 2023; 21(6): 575–583.</p></div>","PeriodicalId":48599,"journal":{"name":"Journal of Integrative Medicine-Jim","volume":"21 6","pages":"Pages 575-583"},"PeriodicalIF":4.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}