Sofya M. Bruman, Valeria M. Zubareva, Tatiana E. Shugaeva, Anna S. Lapashina, Boris A. Feniouk
{"title":"Activation of Bacterial F-ATPase by LDAO: Deciphering the Molecular Mechanism","authors":"Sofya M. Bruman, Valeria M. Zubareva, Tatiana E. Shugaeva, Anna S. Lapashina, Boris A. Feniouk","doi":"10.1134/S0006297924602600","DOIUrl":"10.1134/S0006297924602600","url":null,"abstract":"<p>Proton F<sub>O</sub>F<sub>1</sub> ATP synthase catalyzes the formation of ATP from ADP and inorganic phosphate coupled with transmembrane proton transfer using the energy of the protonmotive force (<i>pmf</i>). As <i>pmf</i> decreases, the direction of the reaction is reversed and the enzyme generates <i>pmf</i>, transferring protons across the membrane using the energy of ATP hydrolysis. ATPase activity of the enzyme can be suppressed by ADP in a non-competitive manner (ADP-inhibition), and in a number of bacteria, it can be inhibited by conformational changes in the regulatory C-terminal domain of the ε subunit. Lauryldimethylamine oxide (LDAO), a zwitterionic detergent, is known to attenuate both of these inhibitory mechanisms, significantly increasing the ATPase activity of the enzyme. For this reason, LDAO is sometimes used for semi-quantitative estimation of the enzyme’s susceptibility to these regulatory mechanisms. However, the binding site of LDAO in ATP synthase remains unknown. The mechanism by which the detergent counteracts ADP-inhibition and the inhibition involving the ε subunit is also unclear. We performed molecular docking and predicted that LDAO binding might occur at the catalytic site of ATP synthase, whether empty or containing nucleotides. Molecular dynamics simulations showed that LDAO could affect the mobility of the loop in the β subunit (residues β404-415 in <i>Escherichia coli</i> ATP synthase) near the catalytic site. Mutagenesis of residue β409 in the <i>E. coli</i> enzyme and the corresponding β419 residue in the <i>Bacillus subtilis</i> ATP synthase revealed that the type of side chain of this residue indeed affects LDAO-dependent stimulation of ATPase activity. We also found that LDAO activates the enzyme more strongly in the presence of 100 mM sulfate compared to sulfate-free medium. This phenomenon is likely due to the enhancement of ADP-inhibition of the enzyme by sulfate.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 3","pages":"374 - 388"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alina O. Makarova, Matvey M. Titov, Daniel V. Kalinovsky, Irina V. Kholodenko, Alexey V. Kibardin, Sergey S. Larin, Elena V. Svirshchevskaya, Sergey M. Deyev, Roman V. Kholodenko
{"title":"Endocytosis Properties of GD2-Specific Antibodies in Tumor Cells","authors":"Alina O. Makarova, Matvey M. Titov, Daniel V. Kalinovsky, Irina V. Kholodenko, Alexey V. Kibardin, Sergey S. Larin, Elena V. Svirshchevskaya, Sergey M. Deyev, Roman V. Kholodenko","doi":"10.1134/S0006297925600395","DOIUrl":"10.1134/S0006297925600395","url":null,"abstract":"<p>Antibody–drug conjugates (ADCs) represent one of the most promising classes of monoclonal antibody-based (mAb) targeted cancer therapies. To date, 15 drugs of this class have received clinical approval, with numerous others under investigation in more than 100 clinical trials. Similarly to unconjugated antibodies, ADCs target various tumor markers including carbohydrate antigens such as glycosphingolipids. Among these targets, ganglioside GD2 is considered the most promising marker. Recent studies have demonstrated significant potential for the anti-GD2 ADCs in clinical application. Internalization of the antigen-antibody complexes and their subsequent transport to cellular lysosomes are critical factors that substantially influence ADC efficacy. However, internalization capacity of the GD2-specific antibodies and mechanisms of endocytosis of their complexes with GD2 have been insufficiently characterized. This study investigated internalization mechanisms of the ganglioside GD2 complexes with several of the most relevant GD2-specific antibody formats, namely full-length antibodies, minibodies, and scFv fragments. It was demonstrated that all used antibody variants successfully internalize into the GD2-positive tumor cells and enter their lysosomal compartments. Full-length antibodies and minibodies exhibited high endocytosis efficiency in the GD2-positive cells, occurring through several pathways, primarily macropinocytosis and caveolae-mediated endocytosis. These findings may be of interest for the development of more effective targeted therapeutics for GD2-positive tumors.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 3","pages":"424 - 435"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexander L. Ksenofontov, Maxim. V. Petoukhov, Georgy S. Peters, Alexander M. Arutyunyan, Lyudmila A. Baratova, Marina V. Arkhipenko, Nikolai A. Nikitin, Olga V. Karpova, Eleonora V. Shtykova
{"title":"Influence of in situ Limited Proteolysis of Potato Virus X on Change in the Structure of Virions According to the Small-Angle X-Ray Scattering Data and Tritium Labeling","authors":"Alexander L. Ksenofontov, Maxim. V. Petoukhov, Georgy S. Peters, Alexander M. Arutyunyan, Lyudmila A. Baratova, Marina V. Arkhipenko, Nikolai A. Nikitin, Olga V. Karpova, Eleonora V. Shtykova","doi":"10.1134/S0006297925600279","DOIUrl":"10.1134/S0006297925600279","url":null,"abstract":"<p>Capsids of the potexvirus family virions are characterized by the presence on the surface of virions of partially disordered N-terminal protein fragments of various lengths. The present study is devoted to studying the effect of <i>in situ</i> removal of the N-terminal domain of coat protein (CP) on structural organization and physicochemical properties of the potato virus X (PVX) virions. The work considers PVX virions containing an intact Ps-form CP, as well as virions including an <i>in situ</i> degraded Pf-form devoid of 19/21 amino acid residues from the N-end (PVXΔN). Synchrotron small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM), tritium bombardment, and several other physicochemical methods were used in the study. Analysis of the images obtained using TEM revealed similarities in the architecture of filamentous PVX and PVXΔN virions. SAXS results demonstrated differences in organization of the capsid of PVX and PVXΔN virions: the latter was characterized by the reduced size of the ordered regions, indicating partial disruption of the structure of the viral protein framework. In addition, based on the SAXS scattering curves, parameters of the spiral packing of virions in solution were calculated, and structural modeling of particles was performed. Modeling results also indicate changes in the structure of the capsid due to removal of the ΔN-peptide. Using information about the secondary structure of the PVX model (PDB ID: 6R7G) and data from our previous studies on tritium labeling of the surface sites of PVX and PVXΔN virions, comparative analysis of the label incorporation profiles into elements of the protein secondary structure was conducted. This approach made it possible to predict localization of the ΔN-peptide above the amino acid residues of neighboring helical subunits (122-129 and 143-153) and demonstrate stabilizing role of this peptide on the overall structure of the virion. Increase in the level of labelling in the C-terminal region after removal of the ΔN-peptide also indicates decrease in the compactness of the virion. In general, the gained knowledge could be useful when using virus-like nanoparticles in biotechnology.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 3","pages":"413 - 423"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilya G. Mikhailov, Milana S. Mikhailova, Anton N. Shuvaev, Yana V. Gorina, Olga S. Belozor
{"title":"RAGE-Mediated Effects of Formaldehyde in Alzheimer’s Disease","authors":"Ilya G. Mikhailov, Milana S. Mikhailova, Anton N. Shuvaev, Yana V. Gorina, Olga S. Belozor","doi":"10.1134/S0006297924604593","DOIUrl":"10.1134/S0006297924604593","url":null,"abstract":"<p>Alzheimer’s disease (AD) remains an incurable pathology with a huge socio-economic impact. One of the known mechanisms of AD pathogenesis is deposition of amyloid plaques as a result of beta-amyloid (Aβ) accumulation. The receptor for glycation end products (RAGE) plays an important role in the Aβ transport across the blood-brain barrier. Ligand interaction with RAGE regulates the expression of the amyloid precursor protein (APP), which plays a key role in the Aβ accumulation. In this review, we discuss the biochemical mechanisms underlying the toxic effects of exogenous formaldehyde in the hippocampus leading to the insulin resistance development, as well as molecular mechanisms of neuroinflammation contributing to the upregulation of RAGE expression. Accumulation of endogenous formaldehyde in the body can be a result of impaired metabolism. However, accumulation of exogenous formaldehyde has much more acute and dangerous consequences. Formaldehyde is one of the most important toxins; its maximum permissible concentration (MPC) is exceeded in many cities of Russia, as well as in the countries of East, South, and Southeast Asia, Central Africa, and North and South Americas. Formaldehyde plays an important role in the pathogenesis of neurodegenerative diseases, as its mechanism of action is closely linked to the increased Aβ accumulation. In people more susceptible to Aβ accumulation (due to age or genetic predisposition), exposure to exogenous formaldehyde may contribute to this process. The role of formaldehyde in neurodegenerative diseases has been already investigated. It was found that the level of air pollution correlates with the incidence of hyperglycemia, but the detailed mechanism of the following development of neurodegeneration remains unclear. This review highlights the importance of studying the relationship between environmental toxins and neurodegenerative diseases, which may lead to the development of therapeutic approaches for the protection of neurons from the effects of toxic substances in individuals susceptible to neurodegenerative diseases.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 3","pages":"334 - 348"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Svyatoslav S. Sokolov, Anna N. Zyrina, Sergey A. Akimov, Fedor F. Severin
{"title":"Interrelationship between the Non-Vesicular Transport of Sterols and Their Distribution between the Rafts and the Non-Raft Phase of the Plasma Membrane","authors":"Svyatoslav S. Sokolov, Anna N. Zyrina, Sergey A. Akimov, Fedor F. Severin","doi":"10.1134/S0006297924604313","DOIUrl":"10.1134/S0006297924604313","url":null,"abstract":"<p>Sterols significantly affect the barrier properties of the membrane, which might explains the fact that their concentration is maximal in the plasma membrane (PM). Together with sphingolipids, sterols form rafts, i.e., bilayer regions whose physicochemical properties differ from those of the surrounding PM. The presence of rafts allows membrane proteins to choose the lipid environment optimal for their functioning (in terms of thickness, rigidity, spontaneous curvature, and lateral pressure profile of the bilayer). The ratio between sterols and sphingolipids in the rafts is close to stoichiometric. Theoretically, excess sterol outside the rafts can critically reduce the degree of order of membrane phospholipids. Sterols are synthesized in the endoplasmic reticulum (ER). The active (against the concentration gradient) transport of sterols from the ER to the PM is driven by proteins of the Osh family, while Lam proteins provide passive reverse transport of sterols from the PM to the ER. Inactivation of Osh proteins does not reduce the total level of sterols in the PM but reduces the rate of their movement inside the PM (the mechanisms underlying this effect remains unclear). Therefore, the vesicular transport of sterols from the ER to the PM is probably more active than the non-vesicular transport carried out by Osh proteins. Since sterols are more rigidly anchored and less sterically accessible in the rafts than outside them, we suggested that Lam proteins transport excess sterols from the non-raft phase of the PM to the ER, and Osh proteins return them back to the PM. In this way, the mutual activity of the Osh and lam proteins provides the rotation of sterols between the non-raft fraction of the PM and rafts, with the enrichment of the latter. It is possible that with a decrease in the sterol concentration in the non-raft fraction of the membrane, the rate of the Lam-dependent transport decreases since the degree of order of phospholipids and, consequently, the strength of retention of sterol molecules in the membrane increases, which might represent a mechanisms maintaining the concentration and distribution of sterols in the PM.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 3","pages":"321 - 333"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dmitry B. Kazansky, Anastasiia A. Kalinina, Ludmila M. Khromykh
{"title":"Memory T Cells: Investigation of Original Models with Transgenic T Cell Receptors","authors":"Dmitry B. Kazansky, Anastasiia A. Kalinina, Ludmila M. Khromykh","doi":"10.1134/S0006297924603940","DOIUrl":"10.1134/S0006297924603940","url":null,"abstract":"<p>This review summarizes the research data on original mouse models developed in the laboratory of regulatory mechanisms in immunity of the Research Institute of Carcinogenesis, N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation. Transfer of the genes of individual α- and β-chains of T cell receptors (TCRs) of memory cells has resulted in production of transgenic animal lines valuable for studying T lymphocyte homeostasis and patterns of formation of their activation profile markers. Investigation of the transgenic models revealed new features of immune selection and tumor progression. In particular, the fundamental property of some TCRs, termed “chain-centricity”, has been confirmed; it involves dominance of one of the TCR chains during recognition of the MHC (major histocompatibility complex)/peptide complex. This property makes it possible to artificially generate a significant pool of immunocompetent T cells so it could be used in adoptive immunotherapy for oncological and infectious diseases. Transfer of the dominant active TCR α-chains provides the possibility for constructing organisms with innate specific immunological resistance to certain pathogens. The results of recent studies indicate that TCR, determining the T lymphocyte relationship with its MHC microenvironment, has an instructive role in formation of its functions and phenotype. One of these functions may be production of cyclophilin A by the cortisone-resistant memory cells localized in thymus. The evidence has been accumulated that expression of TCR with a certain structure and specificity is a sufficient condition for formation of the functional potential of memory cells in a T cell, regardless of its former interaction with antigenic MHC/peptide complexes.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 2","pages":"161 - 172"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yulia G. Levuschkina, Vera B. Dugina, Galina S. Shagieva, Sergey V. Boichuk, Ilya I. Eremin, Natalia V. Khromova, Pavel B. Kopnin
{"title":"Induction of Fibroblast-to-Myofibroblast Differentiation by Changing Cytoplasmic Actin Ratio","authors":"Yulia G. Levuschkina, Vera B. Dugina, Galina S. Shagieva, Sergey V. Boichuk, Ilya I. Eremin, Natalia V. Khromova, Pavel B. Kopnin","doi":"10.1134/S000629792460412X","DOIUrl":"10.1134/S000629792460412X","url":null,"abstract":"<p>Myofibroblasts, which play a crucial role in the tumour microenvironment, represent a promising avenue for research in the field of oncotherapy. This study investigates the potential for the induced differentiation of human fibroblasts into myofibroblasts through downregulation of the γ-cytoplasmic actin (γ-CYA) achieved by RNA interference. A decrease in the γ-CYA expression in human subcutaneous fibroblasts resulted in upregulation of myofibroblast markers, including α-smooth muscle actin (α-SMA), ED-A FN, and type III collagen. These changes were accompanied by notable alterations in cellular morphology, characterized by a significant increase in cell area and the formation of pronounced supermature focal adhesions. Downregulation of γ-CYA resulted in the compensatory increase in expression of the β-cytoplasmic actin and α-SMA, and formation of the characteristic α-SMA-positive stress fibers. In conclusion, our results demonstrate that a decrease in the γ-CYA expression leads to myofibroblastic trans-differentiation of human subcutaneous fibroblasts.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 2","pages":"289 - 298"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanisms of Glucocorticoid Resistance in Nonclassical T Helper Populations Th17.1/Ex-Th17","authors":"Elena M. Kuklina","doi":"10.1134/S0006297924604222","DOIUrl":"10.1134/S0006297924604222","url":null,"abstract":"<p>The nonclassical population of Th1-polarized Th17 lymphocytes (Th17.1/ex-Th17) is currently in the focus of researchers’ attention. These cells possess a unique proinflammatory potential and ability to penetrate blood-tissue barriers and play a key role in the pathogenesis of many inflammatory diseases, primarily autoimmune ones. Th1-polarized Th17 lymphocytes prevail in the autoimmune lesion foci and are considered to be a promising therapeutic target in these pathologies. At the same time, recent studies have shown another distinctive feature of Th1-polarized Th17 – their selective resistance to glucocorticoids. Since glucocorticoids are the first-line drugs for the treatment of the autoimmune disease exacerbation, understanding the causes of this phenomenon is crucial for predicting patients’ response to therapy and improving the treatment effectiveness. This review analyzes the mechanisms of drug resistance of Th1-polarized Th17 cells, compares these mechanisms with those typical of nonpathogenic classical Th17 cells, and discusses the role of glucocorticoid resistance in the body’s response to glucocorticoid therapy.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 2","pages":"188 - 199"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evgeny P. Kazakov, Igor I. Kireev, Sergei A. Golyshev
{"title":"Techniques for Selective Labeling of Molecules and Subcellular Structures for Cryo-Electron Tomography","authors":"Evgeny P. Kazakov, Igor I. Kireev, Sergei A. Golyshev","doi":"10.1134/S0006297924604015","DOIUrl":"10.1134/S0006297924604015","url":null,"abstract":"<p>Electron microscopy (EM) is one of the most efficient methods for studying the fine structure of cells with a resolution thousands of times higher than that of visible light microscopy. The most advanced implementation of electron microscopy in biology is EM tomography of samples stabilized by freezing without water crystallization (cryoET). By circumventing the drawbacks of chemical fixation and dehydration, this technique allows investigating cellular structures in three dimensions at the molecular level, down to resolving individual proteins and their subdomains. However, the problem of efficient identification and localization of objects of interest has not yet been solved, thus limiting the range of targets to easily recognizable or abundant subcellular components. Labeling techniques provide the only way for locating the subject of investigation in microscopic images. CryoET imposes conflicting demands on the labeling system, including the need to introduce into a living cell the particles composed of substances foreign to the cellular chemistry that have to bind to the molecule of interest without disrupting its vital functions and physiology of the cell. This review examines both established and prospective methods for selective labeling of proteins and subcellular structures aimed to enable their localization in cryoET images.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 2","pages":"173 - 187"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anastasia A. Vasilieva, Elena E. Timechko, Anastasia I. Paramonova, Alexey M. Yakimov, Kristina D. Lysova, Marina I.Severina, Diana V. Dmitrenko
{"title":"Differential Expression of hsa-miR-134, hsa-miR-155, hsa-miR-122 as Biomarkers of Epileptogenesis in Patients with Acute Cerebral Injury","authors":"Anastasia A. Vasilieva, Elena E. Timechko, Anastasia I. Paramonova, Alexey M. Yakimov, Kristina D. Lysova, Marina I.Severina, Diana V. Dmitrenko","doi":"10.1134/S0006297924603538","DOIUrl":"10.1134/S0006297924603538","url":null,"abstract":"<p>It is difficult to predict the process of occurrence of an epileptogenic focus in patients who have suffered acute cerebral damage. The aim of our study is to investigate and describe the possibility of using microRNAs as biomarkers of epileptogenesis. Objective of our work was to evaluate expression of hsa-miR-134-5p, hsa-miR-155-5p, and has-miR-122-5p in different groups of patients. Quantitative comparison of relative concentrations of the studied microRNAs in the blood plasma of three groups of patients was carried out: Group I – patients with temporal lobe epilepsy; Group II – patients with potential epileptogenic injuries; Group III – control group. The study showed increased expression of hsa-miR-134-5p in patients with temporal lobe epilepsy, which confirms the published data on its involvement in the mechanisms of epileptogenesis. Hsa-miR-155-5p was overexpressed in the Group II in comparison with other groups, the data indicate involvement in the mechanisms of inflammation, which could indirectly affect occurrence of epilepsy. Hsa-miR-122-5p was overexpressed in two study groups, but the levels were higher in the group of patients with acute cerebral injuries. The obtained results allow us to propose has-miR-122-5p as a potential biomarker of epileptogenesis.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 2","pages":"247 - 258"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}