Biochemistry (Moscow)最新文献_第6页

FOS Promoter is Overactive Outside of Genome Context and Weakly Regulated by Changes in the Na+i/K+i Ratio FOS启动子在基因组外过度活跃，受Na+i/K+i比值变化的调控较弱。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600371

Andrey M. Gorbunov, Dmitrii A. Fedorov, Olga E. Kvitko, Olga D. Lopina, Elizaveta A. Klimanova

引用次数: 0

Accelerated Proteomic Sample Preparation for Accurate Ultrafast Mass Spectrometry-Based Quantitative Analysis of Cell and Tissue Proteomes 加速蛋白质组学样品制备，用于细胞和组织蛋白质组的精确超快质谱定量分析。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600930

Daria D. Emekeeva, Tomiris Kusainova, Leyla A. Garibova, Andrey A. Shelepchikov, Alexey S. Kononikhin, Alexey V. Tretyakov, Olga I. Lavrukhina, Evgeny N. Nikolaev, Mikhail V. Gorshkov, Irina A. Tarasova

{"title":"Accelerated Proteomic Sample Preparation for Accurate Ultrafast Mass Spectrometry-Based Quantitative Analysis of Cell and Tissue Proteomes","authors":"Daria D. Emekeeva, Tomiris Kusainova, Leyla A. Garibova, Andrey A. Shelepchikov, Alexey S. Kononikhin, Alexey V. Tretyakov, Olga I. Lavrukhina, Evgeny N. Nikolaev, Mikhail V. Gorshkov, Irina A. Tarasova","doi":"10.1134/S0006297925600930","DOIUrl":"10.1134/S0006297925600930","url":null,"abstract":"Advances in liquid chromatography/mass spectrometry (LC-MS) have enabled proteome-wide quantitation in minutes, achieving rate of 1000 analyses per day. This necessitates revisiting the rapid sample preparation approaches to match this data acquisition speed. Despite the fact that these approaches have been developed decades ago, their application in quantitative ultrafast proteomics and comprehensive comparison of their performance under different conditions have not been explored. In this study, the ultrasound, microwave irradiation, and elevated temperature-assisted approaches for accelerated protein reduction, alkylation, and trypsin digestion were compared. Validation was carried out with label-free quantitative LC-MS/MS and fragmentation-free DirectMS1 methods of shotgun proteome analyses of Saccharomyces cerevisiae, human cell lines, and winter wheat shoots. These data acquisition methods were applied in ultrafast implementations employing 5 to 16 min LC gradients. Human–yeast proteome mixtures were used as standards to evaluate quantitation accuracy of the sample preparation workflows. Our findings indicate that the reduced time of sample preparation insignificantly decreased efficiency of reduction, alkylation, and digestion, yet, preserved reproducible peptide and protein identification. We also found that the 30-min microwave-assisted and overnight trypsin digestion yielded comparable quantitation accuracy in ultrafast analyses using DirectMS1 method.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 5","pages":"607 - 621"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Circular RNAs in the Frontal Cortex of the Rat Brain in Ischemia–Reperfusion 缺血-再灌注大鼠脑额叶皮层环状rna的差异表达。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600280

Ivan V. Mozgovoy, Yana Yu. Shpetko, Alina E. Denisova, Vasily V. Stavchansky, Margarita A. Vinogradina, Leonid V. Gubsky, Lyudmila V. Dergunova, Svetlana A. Limborska, Ivan B. Filippenkov

{"title":"Differential Expression of Circular RNAs in the Frontal Cortex of the Rat Brain in Ischemia–Reperfusion","authors":"Ivan V. Mozgovoy, Yana Yu. Shpetko, Alina E. Denisova, Vasily V. Stavchansky, Margarita A. Vinogradina, Leonid V. Gubsky, Lyudmila V. Dergunova, Svetlana A. Limborska, Ivan B. Filippenkov","doi":"10.1134/S0006297925600280","DOIUrl":"10.1134/S0006297925600280","url":null,"abstract":"Circular RNAs (circRNAs) are covalently closed non-coding RNAs with an increased metabolic stability, capable of regulating gene expression. CircRNAs can be potentially used as biomarkers and therapeutic targets in various diseases, including ischemic stroke. Transient middle cerebral artery occlusion (tMCAO) is commonly used as a model in stroke transcriptomics. Here, we used genome-wide RNA sequencing to investigate expression profiles of circRNAs in the frontal cortex of rats 24 h after tMCAO. Sixty-four differentially expressed circRNAs (fold change > 1.5; Padj < 0.05) were identified; most of them were upregulated compared to sham-operated animals. According to on the MRI data, the analyzed region of the frontal cortex included the penumbra, an area containing damaged but viable cells, whose survival is crucial for the poststroke recovery. Based on bioinformatics analysis of identified circRNAs and previously obtained data on differential mRNA expression in this brain region, we predicted regulatory circRNA–microRNA–mRNA networks involved in ischemic stroke. Functional analysis of these networks revealed that genes whose expression in ischemia was presumably regulated by circRNAs, are involved in synaptic signaling and inflammatory response. Our data indicate a significant role of circRNA-mediated transcriptome regulation in the penumbra region in ischemia and suggest circRNA as potential targets in the development of new strategies in the therapy of stroke and poststroke complications.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 5","pages":"568 - 581"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex Immune Profiling of Mouse Blood Cells with Highly Sensitive Detection of β-Galactosidase LacZ Reporter 用高灵敏度检测β-半乳糖苷酶LacZ报告蛋白的小鼠血细胞多重免疫谱分析。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600796

Veronika S. Mihailovskaya, Daria A. Bogdanova, Oleg N. Demidov, Stanislav A. Rybtsov

{"title":"Multiplex Immune Profiling of Mouse Blood Cells with Highly Sensitive Detection of β-Galactosidase LacZ Reporter","authors":"Veronika S. Mihailovskaya, Daria A. Bogdanova, Oleg N. Demidov, Stanislav A. Rybtsov","doi":"10.1134/S0006297925600796","DOIUrl":"10.1134/S0006297925600796","url":null,"abstract":"Bacterial β-galactosidase (LacZ) has been widely used as a reporter for the development of mice models to study gene expression or for control of conditional gene deletion. However, high level of endogenous β-galactosidase expression, and low sensitivity of existing substrates for detection of transgenic LacZ activity limited this reporter application for live cell analysis. To overcome this limitation, we evaluated performance of the intracellularly immobilizable fluorescent probe SPiDER-βGal to detect LacZ in major blood cell populations of the reporter mice using multicolor flow cytometry. SPiDER-βGal allows highly sensitive detection of LacZ but also detects endogenous β-galactosidase. Therefore, high background expression of the endogenous β-galactosidase in the myeloid cells impeded advantages of the SPiDER-βGal probe. Application of the proton pump inhibitor – Bafilomycin A1 elevates lysosomal pH and increased specificity of the LacZ detection in the leukocyte populations by suppressing background endogenous β-galactosidase activity. Extending incubation with SPiDER-βGal to 60 minutes also increased sensitivity of the assay tenfold. Thus, lysosomal proton transport inhibitors increase resolution of the LacZ analysis in the cells of reporter animals with high endogenous β-galactosidase activity ex vivo and also enable the use of multicolor FACS analysis and sorting of live LacZ-positive leukocytes for further genetic and functional studies.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 5","pages":"590 - 597"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Antioxidant Activity of Chlorella sorokiniana during Flask-Level Cultivation under Varying Photoheterotrophic Growth Conditions 不同光异养生长条件下小球藻瓶级培养抗氧化活性的评价。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600504

Karla Valeria Gonzalez-Arteaga, Estefanía Morales-Calderón, Julio César Jacuinde-Ruíz, Jesús Alberto Coronado-Reyes, Juan Carlos González-Hernández

{"title":"Evaluation of the Antioxidant Activity of Chlorella sorokiniana during Flask-Level Cultivation under Varying Photoheterotrophic Growth Conditions","authors":"Karla Valeria Gonzalez-Arteaga, Estefanía Morales-Calderón, Julio César Jacuinde-Ruíz, Jesús Alberto Coronado-Reyes, Juan Carlos González-Hernández","doi":"10.1134/S0006297925600504","DOIUrl":"10.1134/S0006297925600504","url":null,"abstract":"The increasing demand for natural antioxidants in the food industry, driven by their health benefits and effectiveness in extending shelf-life of food products, has highlighted potential of the Chlorella sorokiniana as a sustainable source. This study evaluated the effects of environmental growth factors on antioxidant activity of the intracellular extracts of C. sorokiniana. Specifically, the lipid-soluble antioxidants were assessed via the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay, whereas water-soluble and lipid-soluble antioxidants were measured via the 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS•+) assay. The results indicated that the elevated temperatures, low nitrate concentrations, and long photoperiods significantly increase production of antioxidants. For example, at 37°C under a 16 h light/8 h dark cycle and nitrate concentration of 2.524 g L−1, the ABTS•+ assay showed antioxidant activity of up to 13.540 µmol Trolox equivalents mL−1, and the DPPH• assay 2.533 µmol Trolox equivalents mL−1. In particular, reduction in the nitrogen concentration correlated with the increase in antioxidant synthesis, as it was statistically the factor with the greatest effect on antioxidant activity. These findings highlight importance of optimizing growth conditions not only to maximize biomass production but also to increase synthesis of both lipid-soluble and water-soluble antioxidants. These findings position C. sorokiniana as a promising candidate for biotechnological applications, particularly in the development of functional foods and natural preservatives for the food industry.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 5","pages":"635 - 648"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol Kinase Overexpression Suppresses Lipid Synthesis but Increases Mitochondrial Membrane Potential and Thermogenesis Activity in Adipocytes 甘油激酶过表达抑制脂质合成，但增加线粒体膜电位和脂肪细胞的产热活性。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-06-19 DOI: 10.1134/S0006297925600644

Svetlana S. Michurina, Irina B. Beloglazova, Margarita Yu. Agareva, R. Mohammad, Natalia V. Alekseeva, Yelena V. Parfyonova, Iurii S. Stafeev

{"title":"Glycerol Kinase Overexpression Suppresses Lipid Synthesis but Increases Mitochondrial Membrane Potential and Thermogenesis Activity in Adipocytes","authors":"Svetlana S. Michurina, Irina B. Beloglazova, Margarita Yu. Agareva, R. Mohammad, Natalia V. Alekseeva, Yelena V. Parfyonova, Iurii S. Stafeev","doi":"10.1134/S0006297925600644","DOIUrl":"10.1134/S0006297925600644","url":null,"abstract":"Obesity and type 2 diabetes mellitus are among the main factors contributing to the increase in mortality and disability in the modern world. Therefore, it is a priority to develop new methods, including genetic and cellular engineering, to create ectopic thermogenic fat depots capable of dissipating excess energy. In this study, we overexpressed glycerol kinase (GK), a key enzyme of the futile triacylglycerol cycle (TAG cycle) to generate thermogenic adipocytes. The protein-coding sequence of GK was amplified from the mouse liver mRNA and delivered to adipocytes by lentiviral transduction. Adipocyte metabolism was analyzed by radioisotope monitoring of [3H]- and [14C]-labelled glucose analogues. Mitochondrial membrane potential, thermogenesis, and lipid droplet morphology were assessed using fluorescent probes JC-1, ERthermAC, and BODIPY493/503, respectively. Lentiviral delivery of the GK gene increases mRNA expression 130-fold and protein levels by 30% in adipocytes. GK overexpression enhances glucose uptake by adipocytes and suppresses fatty acids synthesis and re-esterification without altering lipid droplet morphology. Increase in the glucose uptake upon GK overexpression is associated with increase in the mitochondrial potential and stimulation of thermogenesis. GK overexpression improves metabolic profile of the adipocytes, which could contribute to elimination of metabolic disorders associated with obesity by increasing utilization of the glucose excess during thermogenesis. Nevertheless, the detailed mechanisms underlying stimulation of these processes require further investigation.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 5","pages":"622 - 634"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depot-Specific Changes in Gene Expression in the Inguinal and Epididymal Adipose Tissues in Response to the High-Intensity Interval Training and Moderate-Intensity Continuous Training in Mice 小鼠高强度间歇训练和中强度连续训练后腹股沟和附睾脂肪组织基因表达的仓库特异性变化。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-05-29 DOI: 10.1134/S0006297925600012

Yisheng Luan, Lingfeng Yuan, Mingyue Wang, Bing Zhang, Yao Liu, Yingzhe Xiong, Yaxin Wang

{"title":"Depot-Specific Changes in Gene Expression in the Inguinal and Epididymal Adipose Tissues in Response to the High-Intensity Interval Training and Moderate-Intensity Continuous Training in Mice","authors":"Yisheng Luan, Lingfeng Yuan, Mingyue Wang, Bing Zhang, Yao Liu, Yingzhe Xiong, Yaxin Wang","doi":"10.1134/S0006297925600012","DOIUrl":"10.1134/S0006297925600012","url":null,"abstract":"Different exercise modes have different mechanisms of impact on white adipose tissue (WAT) and metabolic health. However, specific effects of particular exercise modalities on different adipose tissue depots remain unclear. This study aimed to compare the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on gene expression in the inguinal (subcutaneous) and epididymal (visceral) WAT in mice. The animals were subjected to either HIIT or MICT for 8 weeks, after which their adipose tissues were collected for gene expression analysis. Our results demonstrated depot-specific responses including significantly increased expression of thermogenic and mitochondrial biogenesis markers (UCP-1 and PGC-1α) in the inguinal WAT after HIIT and upregulated expression of lipid metabolism-related genes in the epididymal WAT after MICT. Despite being limited to the endpoint gene expression analysis, these findings provide novel evidence on the exercise modality-specific responses in different adipose depots in vivo. Our study establishes a critical phenotypic foundation for future mechanistic studies, including cell-based approaches, aimed to explore signaling pathways underlying these depot-specific responses. These results have important implications for designing targeted exercise strategies for obesity prevention and metabolic health management.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 4","pages":"544 - 552"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restriction–Modification Systems Specific toward GGATC, GATGC, and GATGG. Part 1. Evolution and Ecology 针对GGATC、GATGC和GATGG的限制修改系统。第1部分。进化与生态学。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-05-29 DOI: 10.1134/S0006297925600115

Sergey Spirin, Ivan Rusinov, Olga Makarikova, Andrei Alexeevski, Anna Karyagina

{"title":"Restriction–Modification Systems Specific toward GGATC, GATGC, and GATGG. Part 1. Evolution and Ecology","authors":"Sergey Spirin, Ivan Rusinov, Olga Makarikova, Andrei Alexeevski, Anna Karyagina","doi":"10.1134/S0006297925600115","DOIUrl":"10.1134/S0006297925600115","url":null,"abstract":"The article presents the results of studies on the evolution of proteins from restriction–modification systems consisting of restriction endonucleases with the REase_AlwI family domain and either two DNA methyltransferases, each with the MethyltransfD12 family domain, or a single DNA methyltransferase with two domains of this family. It was found that all such systems recognized one of the three DNA sequences, namely GGATC, GATGC or GATGG. Based on the sequence similarity, restriction endonucleases of these systems could be attributed to three clades that unambiguously corresponded to the RM system specificity. The DNA methyltransferase domains of these systems were classified into two groups based on sequence similarity, with the two domains of each system belonging to different groups. Within each group, the domains were attributed to three clades according to their specificity. An evidence of multiple interspecific horizontal transfer of entire restriction-modification systems has been found, as well as the transfer of individual genes between the systems (including the transfer of one of DNA methyltransferases accompanied by changes in its specificity). Evolutionary relationships of DNA methyltransferases from the studied systems with other DNA methyltransferases, including orphan DNA methyltransferases, have been revealed.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 4","pages":"502 - 512"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Mesophyll and Bundle Sheath Chloroplasts from Maize Plants Subjected to Salt Stress 盐胁迫下玉米叶肉和束鞘叶绿体的比较分析。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-05-29 DOI: 10.1134/S0006297924603162

Nahida Kh. Aliyeva, Durna R. Aliyeva, Saftar Y. Suleymanov, Fuad H. Rzayev, Eldar K. Gasimov, Irada M. Huseynova

{"title":"Comparative Analysis of Mesophyll and Bundle Sheath Chloroplasts from Maize Plants Subjected to Salt Stress","authors":"Nahida Kh. Aliyeva, Durna R. Aliyeva, Saftar Y. Suleymanov, Fuad H. Rzayev, Eldar K. Gasimov, Irada M. Huseynova","doi":"10.1134/S0006297924603162","DOIUrl":"10.1134/S0006297924603162","url":null,"abstract":"The effect of salt stress was studied in mesophyll (M) and bundle sheath (BS) chloroplasts of maize plants treated with NaCl for 5 days. Pigment content, chlorophyll fluorescence at 77 K, activity of photosystems (PS) I and II, polypeptide composition, and ultrastructure of the thylakoid membranes were investigated in plants grown under different salinity conditions (0, 100, 200, and 250 mM NaCl). Salt treatment caused a decrease in the fluorescence, photochemical activity of PSII and PSI, and protein content of the thylakoids. At high salt concentrations, the F735/F686 fluorescence ratio was reduced in M chloroplasts and increased in BS chloroplasts. The photochemical activity of PSII was reduced in both types of chloroplasts, while no statistically significant difference was observed in the activity of PSI compared to the control. Analysis of the thylakoid membrane protein composition in the M and BS chloroplasts revealed that proteins of the PSII core antenna (47 and 43 kDa) and LHCII (28-24 kDa) were present in both types of membranes, but their content in the BS thylakoids was much lower. The synthesis of the PSI core 68-kDa apoprotein was suppressed in the M membranes. No noticeable changes were observed in the membrane system of BS thylakoids. Salt stress had a greater impact on the ultrastructure of M chloroplasts than on BS chloroplasts and caused formation of granal stacks in BS chloroplasts. These results indicate different response of the M and BS chloroplasts to salt stress.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 4","pages":"522 - 533"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyruvate Kinase PKM2: Basic and Translational Aspects in Molecular Diagnosis of Human Malignancies 丙酮酸激酶PKM2：人类恶性肿瘤分子诊断的基础和转化方面。

IF 2.2 4区生物学

Biochemistry (Moscow) Pub Date : 2025-05-29 DOI: 10.1134/S0006297923601582

Pavel V. Belousov

{"title":"Pyruvate Kinase PKM2: Basic and Translational Aspects in Molecular Diagnosis of Human Malignancies","authors":"Pavel V. Belousov","doi":"10.1134/S0006297923601582","DOIUrl":"10.1134/S0006297923601582","url":null,"abstract":"The M2 isoform of the glycolytic enzyme pyruvate kinase PKM (PKM2) is at a central focus of current research on cancer metabolism. It functions as a highly adaptive metabolic master regulator, orchestrating a switch between modes of (1) energy generation via highly efficient conversion of phosphoenolpyruvate (PEP) to pyruvate yielding ATP, and (2) accumulation of glycolytic intermediates, which are funneled into various biosynthetic pathways (i.e., biosynthesis of amino acids, nucleotides, and fatty acids). Characterized by exceptional functional and regulatory plasticity, PKM2 represents an ideal tool for metabolic adaptation in the PKM2-expressing cells, particularly in malignant tumors exhibiting severely abnormal metabolic demands. Molecular fingerprints of the PKM2-dependent metabolic adaptation, including PKM2 overexpression, shift of the PKM2 tetramer-dimer equilibrium toward the latter, release of the PKM2 dimer into circulation, and spontaneous tumor-associated immune responses against PKM2, may serve as biomarkers in a wide spectrum of human malignancies. This review provides a comprehensive analysis of the potential of PKM2-dependent metabolic fingerprinting in the diagnosis of human malignancies in the context of the fundamental aspects of PKM2 biology and historical and modern notions on the PKM2-driven metabolic adaptation.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 4","pages":"457 - 475"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0